##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424182
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91190809605311	31.0	31.0	33.0	28.0	34.0
2	31.118458586172917	31.0	31.0	34.0	28.0	34.0
3	31.09738272722558	31.0	31.0	34.0	28.0	34.0
4	35.107137973794266	35.0	35.0	37.0	32.0	37.0
5	34.71414864374255	35.0	35.0	37.0	32.0	37.0
6	34.760161911632274	35.0	35.0	37.0	32.0	37.0
7	34.561796587313935	35.0	35.0	37.0	31.0	37.0
8	34.984365201729446	35.0	35.0	37.0	32.0	37.0
9	35.44523812891636	38.0	35.0	39.0	31.0	39.0
10	35.4329085156843	37.0	35.0	39.0	30.0	39.0
11	35.855531352108294	37.0	35.0	39.0	31.0	39.0
12	35.346240528829604	37.0	35.0	39.0	30.0	39.0
13	35.03408442602468	37.0	34.0	39.0	29.0	39.0
14	35.877059846952484	38.0	34.0	40.0	29.0	41.0
15	36.14043735943534	38.0	34.0	40.0	30.0	41.0
16	36.13415467888784	38.0	34.0	40.0	31.0	41.0
17	36.070389596918304	38.0	34.0	40.0	30.0	41.0
18	35.84050714080277	37.0	34.0	40.0	30.0	41.0
19	35.74267885011622	37.0	34.0	40.0	29.0	41.0
20	35.76822448854501	37.0	34.0	40.0	30.0	41.0
21	35.823365913687994	37.0	34.0	40.0	30.0	41.0
22	35.79114153830196	37.0	34.0	40.0	30.0	41.0
23	35.723543196080925	37.0	34.0	40.0	30.0	41.0
24	35.340523643153176	37.0	34.0	40.0	29.0	41.0
25	35.43938922443668	37.0	34.0	40.0	29.0	41.0
26	35.325466427146836	37.0	34.0	40.0	29.0	41.0
27	35.15131240835302	37.0	34.0	40.0	27.0	41.0
28	35.035531917903164	37.0	34.0	40.0	27.0	41.0
29	34.76988886845741	36.0	33.0	40.0	26.0	41.0
30	34.59337737103413	36.0	33.0	40.0	25.0	41.0
31	34.3989018864544	36.0	33.0	39.0	25.0	41.0
32	34.34657293331636	36.0	33.0	39.0	25.0	41.0
33	34.180464989084875	36.0	33.0	39.0	24.0	40.0
34	34.14459359425907	36.0	33.0	39.0	24.0	41.0
35	33.93591194345823	36.0	33.0	39.0	23.0	41.0
36	33.620580316939424	35.0	32.0	39.0	23.0	40.0
37	33.315209037630076	35.0	32.0	39.0	22.0	40.0
38	33.3586856585145	35.0	32.0	39.0	22.0	40.0
39	33.27877420541183	35.0	32.0	39.0	22.0	40.0
40	33.106796139393	35.0	32.0	39.0	21.0	40.0
41	33.10815876204082	35.0	32.0	39.0	21.0	40.0
42	33.16102522030638	35.0	32.0	39.0	21.0	40.0
43	32.99332597800001	35.0	32.0	39.0	21.0	40.0
44	32.944450731054125	35.0	32.0	39.0	21.0	40.0
45	32.708033344177736	35.0	31.0	39.0	20.0	40.0
46	32.446598394085555	35.0	31.0	39.0	20.0	40.0
47	32.40422271572108	35.0	31.0	38.0	19.0	40.0
48	32.33247285363358	35.0	31.0	38.0	19.0	40.0
49	32.17959743694924	35.0	31.0	38.0	18.0	40.0
50	31.980675747674347	35.0	31.0	38.0	17.0	40.0
51	29.9431682626797	34.0	26.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	8.0
10	16.0
11	21.0
12	9.0
13	16.0
14	30.0
15	49.0
16	112.0
17	282.0
18	587.0
19	1103.0
20	1784.0
21	2747.0
22	3558.0
23	4620.0
24	5297.0
25	5941.0
26	6774.0
27	7988.0
28	10079.0
29	12370.0
30	15240.0
31	19814.0
32	25123.0
33	33363.0
34	50925.0
35	52548.0
36	42235.0
37	48274.0
38	47673.0
39	25590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.777317283618824	40.19571787581746	18.340476493580585	22.686488346983136
2	18.19384132282841	40.93431593042609	18.68372538202941	22.18811736471609
3	17.699713802094383	31.864152651456212	27.411582764002247	23.024550782447157
4	22.99201757736066	32.69092040680651	17.698770810642603	26.618291205190225
5	16.200357393760225	41.46333413487607	18.358393331164454	23.977915140199254
6	20.047055273443945	35.50999335191026	24.329650951714125	20.113300422931665
7	67.31662352480775	15.929483099235705	9.674856547425398	7.079036828531149
8	69.78160317976717	8.99778868504557	7.162963067739792	14.057645067447464
9	65.98983455214979	13.441871649433498	13.274019171016215	7.294274627400503
10	42.86226195359539	25.800717616494808	13.5467794484443	17.7902409814655
11	34.97060224149068	22.236209928756995	24.22380016125154	18.569387668500788
12	30.574847589006605	23.327722534195228	30.72831944778421	15.36911042901396
13	23.184152085661346	37.06097854222952	22.020500634161753	17.73436873794739
14	12.92534808171964	36.602213200937335	34.6115582462245	15.860880471118529
15	11.851988061728221	27.490322550226082	36.801184397263434	23.85650499078226
16	13.286042312026442	25.3216779589893	37.82409437458449	23.568185354399763
17	13.390242867448407	25.866491270256635	28.467968937861578	32.27529692443338
18	19.679288607248775	25.99450235983611	27.935414515467414	26.390794517447702
19	21.525430121975944	31.472103955377644	27.371741375164433	19.630724547481975
20	22.874850889476686	31.960573527400975	23.40834830332263	21.75622727979971
21	16.50447213696008	32.82011023570071	25.362933835004785	25.312483792334422
22	16.62258181629584	33.729153995219036	23.784601892583844	25.863662295901285
23	20.572065764223847	30.945207481694176	19.507664163024362	28.97506259105761
24	14.92755468171681	33.891820020651515	26.874549132212117	24.30607616541956
25	17.942769848791322	27.165697742949963	26.998788255984458	27.892744152274258
26	22.195425548467405	33.41608083322725	20.5961120462443	23.79238157206105
27	16.20059314162317	31.71798897642993	25.07084223281516	27.01057564913174
28	23.70020415764931	28.734364022990132	26.635029303459362	20.930402515901196
29	22.322022150869202	30.613745986392633	25.74979607809855	21.314435784639613
30	24.139873922042895	30.844071648490505	22.827937064750508	22.18811736471609
31	29.415439599040035	35.034254164486	15.616881432969809	19.933424803504156
32	28.550480689892545	26.637386782088818	17.33477611025456	27.47735641776407
33	28.065783083676347	28.194029921118762	22.92152896633992	20.818658028864967
34	22.920114479162244	30.28322748254287	19.75967862851323	27.036979409781654
35	28.38404269865294	27.497630733977395	23.481665888698718	20.63666067867095
36	27.541244088622335	30.22075429886228	25.148167531861326	17.08983408065406
37	21.891546553130496	34.35082110980664	19.431517603292924	24.326114733769938
38	24.278965161180814	32.76659547081206	22.996732534619575	19.957706833387554
39	21.19019666086727	27.297952294062455	25.834665308758975	25.677185736311298
40	25.9546609709983	27.16734797799058	22.854105077537472	24.02388597347365
41	20.85614193907332	24.140581165631733	28.65161652309622	26.351660372198726
42	30.525576285650967	22.429523176372406	21.546411681778103	25.49848885619852
43	23.567713858673873	23.576200781739914	27.088843939629687	25.767241419956527
44	22.130123390431468	24.704490053797663	23.897996614660688	29.267389941110185
45	25.58618706121429	29.832241820727894	22.91893573984752	21.662635378210297
46	19.77877420541183	24.362891400389454	32.628447223125924	23.229887171072793
47	22.36704999269182	26.11874148360845	25.016855972200613	26.49735255149912
48	22.21923608262491	20.332545935471096	34.738862092215136	22.709355889688858
49	25.0147342414341	18.231325233036763	29.262674983851273	27.49126554167787
50	20.426373584923454	22.72986595376513	24.873992767255565	31.96976769405585
51	18.871852176660017	22.355734095270428	32.38774865505844	26.384665073011114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5059.0
1	4054.0
2	3049.0
3	2159.5
4	1270.0
5	1221.5
6	1173.0
7	1279.0
8	1385.0
9	1511.0
10	1637.0
11	1726.5
12	1816.0
13	1852.5
14	1889.0
15	1944.0
16	1999.0
17	1873.5
18	1748.0
19	1681.5
20	1615.0
21	1565.0
22	1515.0
23	1505.5
24	1496.0
25	1391.5
26	1653.0
27	2019.0
28	1842.5
29	1666.0
30	1961.0
31	2256.0
32	3047.0
33	3838.0
34	4368.0
35	4898.0
36	5101.5
37	5305.0
38	6160.5
39	7016.0
40	9469.0
41	11922.0
42	18869.5
43	25817.0
44	42816.5
45	59816.0
46	57378.5
47	54941.0
48	50993.0
49	47045.0
50	42691.0
51	38337.0
52	34006.0
53	29675.0
54	26103.0
55	22531.0
56	19828.0
57	17125.0
58	15534.0
59	13943.0
60	13140.5
61	12338.0
62	10970.0
63	9602.0
64	8775.0
65	7948.0
66	6856.5
67	5765.0
68	4882.5
69	4000.0
70	3471.0
71	2942.0
72	2529.0
73	2116.0
74	1863.5
75	1421.0
76	1231.0
77	918.5
78	606.0
79	520.0
80	434.0
81	327.5
82	221.0
83	184.5
84	148.0
85	107.5
86	67.0
87	52.0
88	37.0
89	25.0
90	13.0
91	7.5
92	2.0
93	5.5
94	9.0
95	5.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	424182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.34220845508793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53279178905312	26.0460833951175
2	8.885510318559923	5.747540539053995
3	3.3693616712502594	3.26917792596478
4	1.6333458744935179	2.113040510085089
5	0.9433159432093253	1.5254460437141941
6	0.6130307527403905	1.1896061036705505
7	0.39521338330963435	0.8947451539068533
8	0.2773160445148479	0.7175210655711725
9	0.2070568143694488	0.6027007187144843
>10	2.08000836266185	15.932796482326273
>50	0.7674731239392699	17.852985311482378
>100	0.2824066976554416	12.323573910950627
>500	0.005121364983388836	1.273269545800298
>1k	0.005121364983388836	3.251439663416074
>5k	0.0029264942762221922	7.2600736302257465
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	9075	2.1394118562315234	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	8636	2.035918544398395	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGT	7762	1.8298749121839213	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	5209	1.2280106180837471	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4331	1.0210239944174906	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	1907	0.44957117463730195	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	1835	0.43259732850521715	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	1690	0.3984138883781019	No Hit
AACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1539	0.3628159610733129	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	1437	0.3387696790528594	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1002	0.23621935867151364	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	972	0.229146922783145	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTAT	948	0.22348897407245003	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	873	0.2058078843515284	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	763	0.1798756194275099	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	746	0.1758679057574343	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	570	0.13437628187900474	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	509	0.1199956622393218	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	474	0.11174448703622501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.714957258912448E-4	0.0	0.0	1.6247742714212297	0.0
2	4.714957258912448E-4	0.0	0.0	2.013993993144452	0.0
3	4.714957258912448E-4	0.0	0.0	2.8442979664389343	0.0
4	4.714957258912448E-4	0.0	0.0	7.2935673838116655	0.0
5	4.714957258912448E-4	0.0	0.0	7.92772913513539	0.0
6	4.714957258912448E-4	0.0	0.0	10.429249708851389	0.0
7	4.714957258912448E-4	0.0	0.0	12.543200795884784	0.0
8	4.714957258912448E-4	0.0	0.0	13.995407631629819	0.0
9	4.714957258912448E-4	0.0	0.0	17.57453168687026	0.0
10	4.714957258912448E-4	0.0	0.0	20.209957046739373	0.0
11	4.714957258912448E-4	0.0	0.0	24.807276122041955	0.0
12	4.714957258912448E-4	0.0	0.0	26.205260949309494	0.0
13	4.714957258912448E-4	0.0	0.0	26.97073426029393	0.0
14	4.714957258912448E-4	0.0	0.0	27.736443319141312	0.0
15	4.714957258912448E-4	0.0	0.0	28.143815626311348	0.0
16	4.714957258912448E-4	0.0	0.0	28.852002206599998	0.0
17	4.714957258912448E-4	0.0	0.0	29.60521662871126	0.0
18	4.714957258912448E-4	0.0	0.0	30.949215195364253	0.0
19	4.714957258912448E-4	0.0	0.0	31.359652224752583	0.0
20	4.714957258912448E-4	0.0	0.0	31.81110938229345	0.0
21	4.714957258912448E-4	0.0	0.0	32.205515557001476	0.0
22	4.714957258912448E-4	0.0	0.0	32.55677987279045	0.0
23	4.714957258912448E-4	0.0	0.0	32.908279936442376	0.0
24	4.714957258912448E-4	0.0	0.0	33.163830619875434	0.0
25	4.714957258912448E-4	0.0	0.0	33.38024715805951	0.0
26	4.714957258912448E-4	0.0	0.0	33.58511205095926	0.0
27	4.714957258912448E-4	0.0	0.0	33.860937050605635	0.0
28	4.714957258912448E-4	0.0	0.0	34.079239571693286	0.0
29	4.714957258912448E-4	0.0	0.0	34.287640682537216	0.0
30	4.714957258912448E-4	0.0	0.0	34.79992078871805	0.0
31	4.714957258912448E-4	0.0	0.0	35.03307542517127	0.0
32	4.714957258912448E-4	0.0	0.0	35.23228236936032	0.0
33	4.714957258912448E-4	0.0	0.0	35.46944471948362	0.0
34	4.714957258912448E-4	0.0	0.0	35.69010471920072	0.0
35	4.714957258912448E-4	0.0	0.0	35.942590680415485	0.0
36	4.714957258912448E-4	0.0	0.0	36.12364503915772	0.0
37	4.714957258912448E-4	0.0	0.0	36.304935145762904	0.0
38	4.714957258912448E-4	0.0	0.0	36.49754114978948	0.0
39	4.714957258912448E-4	0.0	0.0	36.71490067942534	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	30	2.162251E-6	45.000004	39
TCACGAG	20	7.028247E-4	45.000004	1
AAGTATA	20	7.028247E-4	45.000004	32
TTCTAAC	40	6.7975634E-9	45.000004	20
AGCGTAC	20	7.028247E-4	45.000004	34
ACTATCG	30	2.162251E-6	45.000004	1
GTACCGA	20	7.028247E-4	45.000004	8
AGATCTC	20	7.028247E-4	45.000004	44
ACCGGCA	20	7.028247E-4	45.000004	3
CTCGTTA	20	7.028247E-4	45.000004	15
CGAGTGA	20	7.028247E-4	45.000004	17
CGAGTAT	20	7.028247E-4	45.000004	32
TAAAGCC	20	7.028247E-4	45.000004	26
ACGCATT	20	7.028247E-4	45.000004	44
ACGTTGC	20	7.028247E-4	45.000004	25
ACTGACA	20	7.028247E-4	45.000004	26
ACGTTAG	20	7.028247E-4	45.000004	1
GACACGT	30	2.162251E-6	45.000004	19
TAGACCG	20	7.028247E-4	45.000004	1
GTTTAGC	20	7.028247E-4	45.000004	35
>>END_MODULE