SRR3548725_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518953
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	7472	1.4398221033503997	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	6838	1.3176530437245764	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT	5840	1.125342757436608	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	3498	0.6740494803961052	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2956	0.5696084231134612	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1475	0.2842261245238008	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1256	0.24202577111992798	No Hit
AACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	1176	0.22661011690846763	TruSeq Adapter, Index 15 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	997	0.19211759061032502	TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTAT	896	0.17265532716835627	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	736	0.14182401874543554	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.13141845215269976	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	671	0.12929879969862396	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	640	0.12332523369168306	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	590	0.11369044980952034	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTAC	20	7.029705E-4	45.000004	30
GTCGACA	20	7.029705E-4	45.000004	27
ACGTTCC	20	7.029705E-4	45.000004	21
GGGTCGA	20	7.029705E-4	45.000004	7
TTTCGCG	20	7.029705E-4	45.000004	1
GACGTAA	20	7.029705E-4	45.000004	31
TAGAACG	40	6.8030204E-9	45.000004	1
TTCGTCG	20	7.029705E-4	45.000004	18
CTAAGTA	20	7.029705E-4	45.000004	33
ATAGACG	20	7.029705E-4	45.000004	1
TGAGTCG	20	7.029705E-4	45.000004	26
CGACACG	20	7.029705E-4	45.000004	1
TAGCCGT	20	7.029705E-4	45.000004	44
GCGCAAC	20	7.029705E-4	45.000004	8
ACCTCCG	20	7.029705E-4	45.000004	29
TTGAGTA	30	2.1631913E-6	45.000004	13
AACGGTA	20	7.029705E-4	45.000004	4
AAACGGT	20	7.029705E-4	45.000004	3
AATGCGG	40	6.8030204E-9	45.000004	2
TCAATCA	30	2.1631913E-6	45.000004	29