##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518953
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146263727158335	31.0	31.0	34.0	28.0	34.0
2	31.35456004686359	31.0	31.0	34.0	28.0	34.0
3	31.279131250806913	31.0	31.0	34.0	28.0	34.0
4	35.28461536979264	37.0	35.0	37.0	33.0	37.0
5	34.94061311910713	35.0	35.0	37.0	32.0	37.0
6	34.983879079608364	36.0	35.0	37.0	32.0	37.0
7	34.87616990363289	36.0	35.0	37.0	32.0	37.0
8	35.24172709281958	37.0	35.0	37.0	32.0	37.0
9	35.73216649677331	39.0	35.0	39.0	32.0	39.0
10	35.63761843558087	37.0	35.0	39.0	30.0	39.0
11	36.0628187909117	37.0	35.0	39.0	31.0	39.0
12	35.74255664771184	37.0	35.0	39.0	31.0	39.0
13	35.553077060928445	37.0	35.0	39.0	30.0	39.0
14	36.522885502155304	38.0	35.0	40.0	31.0	41.0
15	36.65403996122963	38.0	35.0	40.0	31.0	41.0
16	36.59480145600854	38.0	35.0	40.0	31.0	41.0
17	36.562627058712444	38.0	35.0	40.0	31.0	41.0
18	36.33376047541878	38.0	35.0	40.0	30.0	41.0
19	36.31999236925117	38.0	35.0	40.0	30.0	41.0
20	36.36045075372914	38.0	35.0	40.0	30.0	41.0
21	36.4409262495833	38.0	35.0	40.0	31.0	41.0
22	36.41142068742256	38.0	35.0	40.0	31.0	41.0
23	36.308848778213054	38.0	35.0	40.0	31.0	41.0
24	35.92984528464042	38.0	34.0	40.0	30.0	41.0
25	36.05195653556295	38.0	34.0	40.0	30.0	41.0
26	35.92183299836401	38.0	34.0	40.0	30.0	41.0
27	35.85350311107172	38.0	34.0	40.0	30.0	41.0
28	35.666644185504275	38.0	34.0	40.0	29.0	41.0
29	35.281620878962066	37.0	34.0	40.0	27.0	41.0
30	35.42322907854854	37.0	34.0	40.0	28.0	41.0
31	35.224182151370165	37.0	34.0	40.0	27.0	41.0
32	35.0692047256688	37.0	34.0	40.0	27.0	41.0
33	35.077650577219906	37.0	34.0	40.0	27.0	41.0
34	34.7391825463963	37.0	33.0	40.0	25.0	41.0
35	34.729445633804986	37.0	33.0	40.0	25.0	41.0
36	34.41591242366842	37.0	33.0	40.0	24.0	41.0
37	34.27724283316601	37.0	33.0	40.0	24.0	41.0
38	34.19768456873744	37.0	33.0	40.0	24.0	41.0
39	33.875032999134795	36.0	33.0	40.0	23.0	41.0
40	33.7793364717036	36.0	33.0	40.0	23.0	41.0
41	33.74420612271246	36.0	33.0	40.0	23.0	41.0
42	33.774993111129525	36.0	33.0	40.0	23.0	41.0
43	33.70120222833282	36.0	33.0	40.0	22.0	41.0
44	33.67313802984085	36.0	33.0	40.0	22.0	41.0
45	33.32597749699877	35.0	32.0	39.0	21.0	41.0
46	33.04306170308294	35.0	31.0	39.0	20.0	41.0
47	32.89655517937077	35.0	31.0	39.0	20.0	40.0
48	32.76667636568244	35.0	31.0	39.0	19.0	40.0
49	32.64268633190289	35.0	31.0	39.0	18.0	40.0
50	32.48555456852547	35.0	31.0	39.0	18.0	40.0
51	30.595398812609233	34.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	4.0
11	4.0
12	7.0
13	15.0
14	31.0
15	60.0
16	115.0
17	235.0
18	483.0
19	947.0
20	1691.0
21	2550.0
22	3607.0
23	4739.0
24	5729.0
25	6730.0
26	7989.0
27	9562.0
28	11408.0
29	13727.0
30	16740.0
31	21229.0
32	26291.0
33	34890.0
34	53488.0
35	55433.0
36	50923.0
37	63933.0
38	74479.0
39	51904.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.420448479920147	37.580859923731055	18.68011168641476	24.31857990993404
2	20.73097178357192	37.44154094879498	20.042662823030216	21.784824444602883
3	20.09970074361262	29.668197312666077	26.29467408416562	23.937427859555683
4	23.081088268109056	31.724067497441965	17.815486180829478	27.3793580536195
5	17.042198426447097	38.584033621541835	18.196060144174904	26.177707807836164
6	20.416492437658132	35.47469616709027	23.624297383385393	20.4845140118662
7	75.0293379169212	11.768888512061785	7.417242023844163	5.784531547172865
8	76.91891173189094	6.387090931163324	5.861995209585453	10.832002127360282
9	71.7064936516409	11.908207486997858	10.17298290982035	6.212315951540891
10	41.17251465932368	30.253028694313354	12.955893886344235	15.618562760018731
11	32.98776575142643	24.390455397694975	24.14997119199619	18.4718076588824
12	29.837769508992146	22.976069123793486	30.021023098430877	17.16513826878349
13	23.635088341333415	32.01368910093978	24.01893813119878	20.332284426528027
14	16.643125678047916	32.6673128395057	32.42547976406341	18.264081718382975
15	15.758459821987733	28.020071181783322	33.60246496310842	22.619004033120532
16	16.802870394814175	25.641821128310273	34.80469329592468	22.750615180950877
17	16.241162494484087	25.323680564521254	28.649415264966194	29.785741676028465
18	20.216088932909145	25.99175647891042	27.92218177754055	25.869972810639887
19	21.57979624359046	30.689291708497684	27.836817592344588	19.89409445556727
20	24.471965669338072	29.854341337269464	24.06460700680023	21.609085986592234
21	21.014812516740434	29.13963306889063	25.359136569207617	24.486417845161316
22	20.00971186215322	30.046458927879787	25.09687775193515	24.846951458031846
23	21.668436255306357	28.644212481669822	21.583457461465684	28.10389380155814
24	17.818954703027057	32.314872445096185	26.73729605571217	23.128876796164587
25	19.428541698381164	26.478698456314927	27.023834528367697	27.068925316936216
26	21.529309976047927	30.710102841683156	23.96633221120217	23.794254971066746
27	19.306372638755338	28.28888165209566	27.076055057009018	25.328690652139983
28	23.57072798500057	26.696637267729447	27.235992469452917	22.496642277817067
29	19.951132376149673	28.829393027884993	29.403048060228958	21.816426535736376
30	27.919869429408827	25.16567010885379	23.532188849471915	23.382271612265466
31	27.913317776368956	27.895975165381063	20.31532720689542	23.875379851354555
32	26.565218815576745	28.39602044886531	21.44259692110846	23.59616381444948
33	31.632729746239065	24.150356583351478	22.327840864201576	21.889072806207885
34	21.255104026761575	26.1894622441724	27.158143415685043	25.39729031338098
35	26.839039373507813	27.274338909303925	24.79511632074581	21.091505396442454
36	26.599518646197247	23.787125230993944	29.180484552551	20.432871570257806
37	24.663505172915468	29.34851518345592	22.559461068728766	23.428518574899844
38	25.152759498451687	27.69017618165807	23.977123169150193	23.179941150740046
39	22.104121182457757	26.730166315639377	27.47454971837527	23.691162783527602
40	25.973450389534314	27.565116686867597	23.10902914136733	23.35240378223076
41	20.21088614961278	22.533832543602212	32.37846201871846	24.87681928806655
42	25.861879592178866	26.298527997718484	22.9899432125838	24.84964919751885
43	23.09361348715587	22.831740061238687	27.89558977402578	26.179056677579666
44	22.05035908839529	23.908908899264482	25.794436105003726	28.246295907336506
45	24.940023470333536	28.447277499118417	24.29430025455099	22.318398775997057
46	21.163573579881028	24.56002759402104	32.005981273834045	22.270417552263886
47	23.076270876167975	25.295161604230053	25.04851113684669	26.58005638275528
48	22.689530651137964	21.89234863272782	32.343584101065034	23.07453661506919
49	23.179555759384762	22.304910078562028	28.898570776158923	25.61696338589429
50	20.742148132875233	23.093228095800583	26.559052553892165	29.60557121743202
51	20.950066769052302	23.155854191034642	29.890568124666395	26.003510915246657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3559.0
1	2870.0
2	2181.0
3	1582.0
4	983.0
5	940.0
6	897.0
7	1005.0
8	1113.0
9	1230.5
10	1348.0
11	1406.5
12	1465.0
13	1522.0
14	1579.0
15	1488.0
16	1397.0
17	1528.5
18	1660.0
19	1607.0
20	1554.0
21	1508.5
22	1463.0
23	1838.5
24	2214.0
25	2348.0
26	2758.5
27	3035.0
28	3325.5
29	3616.0
30	4968.5
31	6321.0
32	7314.0
33	8307.0
34	8406.0
35	8505.0
36	9457.0
37	10409.0
38	11173.0
39	11937.0
40	15087.5
41	18238.0
42	23839.0
43	29440.0
44	43544.0
45	57648.0
46	57895.0
47	58142.0
48	55512.5
49	52883.0
50	48781.0
51	44679.0
52	40269.5
53	35860.0
54	31948.5
55	28037.0
56	25820.0
57	23603.0
58	22051.0
59	20499.0
60	19177.0
61	17855.0
62	16095.5
63	14336.0
64	13032.5
65	11729.0
66	10151.0
67	8573.0
68	7711.0
69	6849.0
70	5995.0
71	5141.0
72	4507.5
73	3874.0
74	2995.5
75	1683.5
76	1250.0
77	1151.0
78	1052.0
79	761.0
80	470.0
81	402.5
82	335.0
83	251.0
84	167.0
85	118.5
86	70.0
87	54.5
88	39.0
89	29.5
90	20.0
91	15.5
92	11.0
93	7.0
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.444916423930117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23845972771161	24.428532003950625
2	8.721196565153639	5.310322010855379
3	3.0795121623898503	2.812664712313059
4	1.4259261542367463	1.7364881036973534
5	0.8649090054407158	1.3166041192473557
6	0.5320785384163027	0.9719451979830737
7	0.3784957072473879	0.8066289121774133
8	0.2602079747262556	0.6337608034704779
9	0.2148933170341243	0.5888168169448529
>10	2.9714657506547533	22.404758187370508
>50	1.0859967864247853	23.170468150237934
>100	0.21732644877546903	8.907878993283196
>500	0.004448202167918957	1.008336923659804
>1k	0.003177287262799254	2.0044855844280947
>5k	0.0019063723576795526	3.898309480380862
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	7472	1.4398221033503997	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	6838	1.3176530437245764	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT	5840	1.125342757436608	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	3498	0.6740494803961052	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2956	0.5696084231134612	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1475	0.2842261245238008	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1256	0.24202577111992798	No Hit
AACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	1176	0.22661011690846763	TruSeq Adapter, Index 15 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	997	0.19211759061032502	TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTAT	896	0.17265532716835627	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	736	0.14182401874543554	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.13141845215269976	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	671	0.12929879969862396	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	640	0.12332523369168306	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	590	0.11369044980952034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.926956776432548E-4	0.0	0.0	1.039207789530073	0.0
2	1.926956776432548E-4	0.0	0.0	1.260037036109243	0.0
3	1.926956776432548E-4	0.0	0.0	1.869533464494858	0.0
4	1.926956776432548E-4	0.0	0.0	5.0118218798234135	0.0
5	1.926956776432548E-4	0.0	0.0	5.443074806389018	0.0
6	1.926956776432548E-4	0.0	0.0	7.0933205897258516	0.0
7	1.926956776432548E-4	0.0	0.0	8.489015382895946	0.0
8	1.926956776432548E-4	0.0	0.0	9.483710470890427	0.0
9	1.926956776432548E-4	0.0	0.0	12.141369256946197	0.0
10	1.926956776432548E-4	0.0	0.0	13.832851915298688	0.0
11	1.926956776432548E-4	0.0	0.0	17.265340021157986	0.0
12	1.926956776432548E-4	0.0	0.0	18.193747796043187	0.0
13	1.926956776432548E-4	0.0	0.0	18.723853605239782	0.0
14	1.926956776432548E-4	0.0	0.0	19.310804639341136	0.0
15	1.926956776432548E-4	0.0	0.0	19.62143007170206	0.0
16	1.926956776432548E-4	0.0	0.0	20.138047183463627	0.0
17	1.926956776432548E-4	0.0	0.0	20.713436476906388	0.0
18	1.926956776432548E-4	0.0	0.0	21.663618863365276	0.0
19	1.926956776432548E-4	0.0	0.0	21.99062342832588	0.0
20	1.926956776432548E-4	0.0	0.0	22.363104173210292	0.0
21	1.926956776432548E-4	0.0	0.0	22.698394652309553	0.0
22	1.926956776432548E-4	0.0	0.0	23.042934523935692	0.0
23	1.926956776432548E-4	0.0	0.0	23.367626740764578	0.0
24	1.926956776432548E-4	0.0	0.0	23.613699121115015	0.0
25	1.926956776432548E-4	0.0	0.0	23.813139147475784	0.0
26	1.926956776432548E-4	0.0	0.0	24.005256738086107	0.0
27	1.926956776432548E-4	0.0	0.0	24.262120076384566	0.0
28	1.926956776432548E-4	0.0	0.0	24.48198584457552	0.0
29	1.926956776432548E-4	0.0	0.0	24.70570552631934	0.0
30	1.926956776432548E-4	0.0	0.0	25.122698972739343	0.0
31	3.853913552865096E-4	0.0	0.0	25.36703709199099	0.0
32	3.853913552865096E-4	0.0	0.0	25.584590512050223	0.0
33	3.853913552865096E-4	0.0	0.0	25.807346715405828	0.0
34	3.853913552865096E-4	0.0	0.0	26.011026046674747	0.0
35	3.853913552865096E-4	0.0	0.0	26.26866016768378	0.0
36	3.853913552865096E-4	0.0	0.0	26.476771499538494	0.0
37	3.853913552865096E-4	0.0	0.0	26.683341265972064	0.0
38	3.853913552865096E-4	0.0	0.0	26.88914024969506	0.0
39	3.853913552865096E-4	0.0	0.0	27.102454364846142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTAC	20	7.029705E-4	45.000004	30
GTCGACA	20	7.029705E-4	45.000004	27
ACGTTCC	20	7.029705E-4	45.000004	21
GGGTCGA	20	7.029705E-4	45.000004	7
TTTCGCG	20	7.029705E-4	45.000004	1
GACGTAA	20	7.029705E-4	45.000004	31
TAGAACG	40	6.8030204E-9	45.000004	1
TTCGTCG	20	7.029705E-4	45.000004	18
CTAAGTA	20	7.029705E-4	45.000004	33
ATAGACG	20	7.029705E-4	45.000004	1
TGAGTCG	20	7.029705E-4	45.000004	26
CGACACG	20	7.029705E-4	45.000004	1
TAGCCGT	20	7.029705E-4	45.000004	44
GCGCAAC	20	7.029705E-4	45.000004	8
ACCTCCG	20	7.029705E-4	45.000004	29
TTGAGTA	30	2.1631913E-6	45.000004	13
AACGGTA	20	7.029705E-4	45.000004	4
AAACGGT	20	7.029705E-4	45.000004	3
AATGCGG	40	6.8030204E-9	45.000004	2
TCAATCA	30	2.1631913E-6	45.000004	29
>>END_MODULE