FastQCFastQC Report
Sat 18 Jun 2016
SRR3548724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498130
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36550.7337442033204183No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT35110.70483608696525No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC17870.3587416939353181No Hit
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT17310.34749964868608596No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT16350.3282275711159737No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT14760.29630819264047537No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT12720.25535502780398694No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8490.17043743601067995No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC8280.16622166904221788No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC7030.1411278180394676No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT7030.1411278180394676No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.1379158051111156No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6160.12366249774155341No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5820.11683697026880534No Hit
CCTAAAGGGATATAAGCACTCTCCAAGCAGTTGGGGTGTGAATCATACCTG5340.10720093148374923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.029432E-445.0000041
CGTGGGA302.1630149E-645.0000044
CCTTATT207.029432E-445.00000418
GGTACGC302.1630149E-645.00000438
CTCGTTA207.029432E-445.00000411
TAAAGCG302.1630149E-645.0000041
ACGTTAG207.029432E-445.00000431
TTAGCCG207.029432E-445.00000415
AACCCGC207.029432E-445.00000443
ACCGCAA207.029432E-445.00000440
AGCTTCA207.029432E-445.00000412
TTCACGA207.029432E-445.00000421
TTGTCAC207.029432E-445.00000434
TGAACGA207.029432E-445.00000428
TGTATCC207.029432E-445.00000433
TTCGTAC302.1630149E-645.00000411
GATCAAT207.029432E-445.00000439
CGACATA207.029432E-445.00000414
CTAAGGT207.029432E-445.00000433
TATGCTA207.029432E-445.00000436