##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498130
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300993716499708	31.0	31.0	34.0	30.0	34.0
2	31.475988195852487	31.0	31.0	34.0	30.0	34.0
3	31.69498122979945	31.0	31.0	34.0	30.0	34.0
4	35.548862746672555	37.0	35.0	37.0	33.0	37.0
5	35.14646578202477	37.0	35.0	37.0	32.0	37.0
6	35.116760684961754	37.0	35.0	37.0	32.0	37.0
7	35.10489831971574	37.0	35.0	37.0	32.0	37.0
8	35.414534358500795	37.0	35.0	37.0	33.0	37.0
9	36.195856503322425	39.0	37.0	39.0	32.0	39.0
10	35.92797462509787	38.0	35.0	39.0	30.0	39.0
11	36.21805151265734	38.0	35.0	39.0	32.0	39.0
12	35.8624074036898	37.0	35.0	39.0	31.0	39.0
13	35.48864553429828	37.0	35.0	39.0	30.0	39.0
14	36.46157830285267	38.0	35.0	40.0	31.0	41.0
15	36.55761548190232	38.0	35.0	40.0	31.0	41.0
16	36.627039126332484	38.0	35.0	40.0	31.0	41.0
17	36.56165860317588	38.0	35.0	40.0	31.0	41.0
18	36.4260935900267	38.0	35.0	40.0	31.0	41.0
19	36.371509445325515	38.0	35.0	40.0	31.0	41.0
20	36.31725252444141	38.0	35.0	40.0	31.0	41.0
21	36.272412823961616	38.0	34.0	40.0	31.0	41.0
22	36.223786963242524	38.0	34.0	40.0	30.0	41.0
23	36.12213879910867	38.0	34.0	40.0	30.0	41.0
24	35.82755304840102	38.0	34.0	40.0	30.0	41.0
25	35.86767711240038	38.0	34.0	40.0	30.0	41.0
26	35.74349266255797	38.0	34.0	40.0	30.0	41.0
27	35.632551743520764	38.0	34.0	40.0	29.0	41.0
28	35.538722823359365	37.0	34.0	40.0	29.0	41.0
29	35.23862445546343	37.0	34.0	40.0	27.0	41.0
30	35.24544998293618	37.0	34.0	40.0	27.0	41.0
31	34.99756087768253	37.0	34.0	40.0	27.0	41.0
32	34.92936382068938	37.0	34.0	40.0	27.0	41.0
33	34.78522474052958	37.0	33.0	40.0	26.0	41.0
34	34.630837331620256	37.0	33.0	40.0	25.0	41.0
35	34.57499447935278	37.0	33.0	40.0	25.0	41.0
36	34.19548712183567	36.0	33.0	40.0	24.0	41.0
37	34.13958805934194	36.0	33.0	40.0	23.0	41.0
38	34.02407604440608	36.0	33.0	40.0	23.0	41.0
39	33.94702989179532	36.0	33.0	40.0	23.0	41.0
40	33.788990825688074	36.0	33.0	39.0	23.0	41.0
41	33.804639351173385	36.0	33.0	39.0	23.0	41.0
42	33.68038865356433	36.0	33.0	39.0	23.0	41.0
43	33.56366209623994	35.0	33.0	39.0	22.0	41.0
44	33.453726938750926	35.0	32.0	39.0	22.0	41.0
45	33.19198402023568	35.0	32.0	39.0	22.0	40.0
46	33.10777106377853	35.0	32.0	39.0	21.0	40.0
47	33.01301467488407	35.0	32.0	39.0	20.0	40.0
48	32.9570694397045	35.0	32.0	39.0	20.0	40.0
49	32.80655250637384	35.0	31.0	39.0	20.0	40.0
50	32.52221508441571	35.0	31.0	39.0	19.0	40.0
51	30.58511834260133	34.0	28.0	37.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	8.0
12	9.0
13	17.0
14	19.0
15	43.0
16	134.0
17	277.0
18	601.0
19	1135.0
20	1787.0
21	2740.0
22	3690.0
23	4923.0
24	5675.0
25	6320.0
26	7167.0
27	8363.0
28	10216.0
29	12112.0
30	15217.0
31	19148.0
32	24621.0
33	33462.0
34	54035.0
35	57635.0
36	48945.0
37	62121.0
38	71048.0
39	46650.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.241844498424108	33.6213438259089	20.084516090177264	25.05229558548973
2	22.15265091441993	34.29807479975107	19.49370646216851	24.05556782366049
3	19.249794230421777	33.73978680264188	19.94599803264208	27.064420934294258
4	19.799249191977996	34.53877501856945	18.115552165097466	27.546423624355086
5	17.443839961455847	36.75586694236445	17.150944532551744	28.649348563627967
6	20.249934755987393	38.57507076465983	18.886435267901952	22.288559211450824
7	77.96579206231306	9.5629654909361	6.901812779796439	5.569429666954409
8	80.47698391986027	7.707225021580712	5.141830445867544	6.673960612691467
9	73.59143195551361	11.649770140324815	9.745849477044146	5.0129484271174185
10	41.858350229859674	30.71688113544657	11.54397446449722	15.880794170196536
11	34.278601971372936	27.691365707746975	21.526509144199306	16.503523176680787
12	32.642482885993616	23.322827374380182	27.556862666372233	16.47782707325397
13	25.58267922028386	32.79886776544276	23.416578001726457	18.201875012546925
14	16.429044626904624	37.06161042298195	28.913938128600968	17.59540682151246
15	15.508602172123743	28.89827956557525	35.74227611266135	19.850842149639654
16	17.389837994097928	26.609519603316407	33.037158974564875	22.963483428020798
17	15.569028165338366	25.8111336398129	28.77481781864172	29.845020376207014
18	21.96033164033485	25.897857988878403	27.252323690602854	24.889486680183886
19	23.148977174633128	32.2052476261217	24.096721739305003	20.549053459940176
20	26.350149559351976	28.710979061690722	23.073294120008832	21.86557725894847
21	21.88585309055869	32.85808925380925	22.816734587356713	22.43932306827535
22	20.373597253728946	29.17330817256539	23.03776122698894	27.415333346716718
23	21.19105454399454	31.316523799008287	20.595426896593256	26.89699476040391
24	19.196193764679904	29.71935037038524	26.414992070343086	24.669463794591774
25	20.02810511312308	29.047838917551644	25.888623451709392	25.035432517615885
26	21.353261196876318	32.62843032943207	23.876899604520908	22.141408869170697
27	18.541746130528175	31.11898500391464	24.05857908578082	26.280689779776363
28	22.505169333306565	28.443578985405416	28.591733081725657	20.45951859956236
29	21.135446570172444	28.40483407945717	27.96779957039327	22.491919779977117
30	23.816272860498263	27.73773914440006	24.487182060907795	23.958805934193887
31	28.93963423202778	29.548310681950497	19.50474775660972	22.007307329412
32	27.846746833156004	25.126774135265894	23.003232088009153	24.02324694356895
33	27.19270070062032	28.691907734928634	21.110754220785736	23.00463734366531
34	21.769819123521973	27.749583442073355	25.55397185473672	24.926625579667956
35	25.255254652399977	26.479232328910125	25.74890088932608	22.516612129363818
36	26.661714813402128	27.466926304378376	25.976351554815007	19.895007327404493
37	25.577058197659248	28.869572200028102	22.425872764137875	23.127496838174775
38	24.585148455222534	30.31076225081806	23.838355449380682	21.265733844578723
39	23.293919258025014	27.419950615301225	25.441752153052416	23.844377973621345
40	26.542468833437056	27.93588019191777	21.68128801718427	23.840362957460904
41	22.262662357215987	24.410696003051413	26.455342982755504	26.871298656977093
42	27.34406680986891	25.19041214140887	22.848653965832213	24.61686708289001
43	23.473791982012727	23.49025354827053	25.9835785839038	27.05237588581294
44	23.669925521450224	24.2508983598659	24.271977194708207	27.80719892397567
45	25.08822998012567	26.960632766546887	25.21590749402766	22.73522975929978
46	21.7664063597856	25.774396241944874	30.021480336458357	22.437717061811174
47	22.806697046955616	26.209824744544598	25.129584646578202	25.853893561921588
48	23.73757854375364	23.002429084777066	30.92445747094132	22.335534900527975
49	24.32015738863349	20.874671270551865	28.467869833176078	26.337301507638568
50	20.59643065063337	24.399052456186133	26.18593539838998	28.818581494790518
51	21.232810712063117	24.379579627808003	28.37632746471804	26.011282195410836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4295.0
1	3465.0
2	2635.0
3	1898.0
4	1161.0
5	1169.0
6	1177.0
7	1274.5
8	1372.0
9	1526.5
10	1681.0
11	1743.0
12	1805.0
13	1844.5
14	1884.0
15	1935.5
16	1987.0
17	1860.5
18	1734.0
19	1843.5
20	1953.0
21	1740.5
22	1528.0
23	1750.0
24	1972.0
25	1853.0
26	2223.0
27	2712.0
28	3067.0
29	3422.0
30	3694.5
31	3967.0
32	5102.5
33	6238.0
34	6340.0
35	6442.0
36	7906.5
37	9371.0
38	9618.5
39	9866.0
40	12781.5
41	15697.0
42	22671.0
43	29645.0
44	36631.5
45	43618.0
46	46679.5
47	49741.0
48	50675.5
49	51610.0
50	50365.0
51	49120.0
52	43899.5
53	38679.0
54	33894.5
55	29110.0
56	26510.5
57	23911.0
58	21659.0
59	19407.0
60	18747.5
61	18088.0
62	17717.5
63	17347.0
64	15096.0
65	12845.0
66	11006.0
67	9167.0
68	7610.5
69	6054.0
70	5439.0
71	4824.0
72	4543.5
73	4263.0
74	3298.5
75	1811.5
76	1289.0
77	1248.5
78	1208.0
79	872.5
80	537.0
81	433.5
82	330.0
83	216.0
84	102.0
85	135.5
86	169.0
87	107.0
88	45.0
89	42.5
90	40.0
91	24.0
92	8.0
93	5.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.920457198683852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92545319071861	24.713315544690246
2	8.881361604547992	5.492315227189207
3	3.2070430390987816	2.974897110743726
4	1.5603684818728674	1.929892274317012
5	0.9074271035322559	1.402903045784739
6	0.5754978552772183	1.067679408122015
7	0.4125919911535865	0.8930273102088955
8	0.27124860051764144	0.6709704594006902
9	0.25878110108378877	0.7201466963900613
>10	2.6627283819784515	19.875731356282895
>50	0.9835120951924495	21.749123011504786
>100	0.3442616496685502	14.386619985101825
>500	0.00518661619086328	1.1029621709170114
>1k	0.00453828916700537	3.020416399346894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3655	0.7337442033204183	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	3511	0.70483608696525	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	1787	0.3587416939353181	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1731	0.34749964868608596	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1635	0.3282275711159737	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT	1476	0.29630819264047537	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	1272	0.25535502780398694	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.17043743601067995	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC	828	0.16622166904221788	No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC	703	0.1411278180394676	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	703	0.1411278180394676	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	687	0.1379158051111156	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	616	0.12366249774155341	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	582	0.11683697026880534	No Hit
CCTAAAGGGATATAAGCACTCTCCAAGCAGTTGGGGTGTGAATCATACCTG	534	0.10720093148374923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0075080802200228E-4	0.0	0.0	0.9933149980928673	0.0
2	2.0075080802200228E-4	0.0	0.0	1.2986569770943328	0.0
3	2.0075080802200228E-4	0.0	0.0	1.6947383213217433	0.0
4	2.0075080802200228E-4	0.0	0.0	3.9082167305723408	0.0
5	2.0075080802200228E-4	0.0	0.0	4.254511874410294	0.0
6	2.0075080802200228E-4	0.0	0.0	6.155822777186678	0.0
7	2.0075080802200228E-4	0.0	0.0	7.632344970188505	0.0
8	2.0075080802200228E-4	0.0	0.0	8.836649067512496	0.0
9	2.0075080802200228E-4	0.0	0.0	10.45831409471423	0.0
10	2.0075080802200228E-4	0.0	0.0	12.87334631521892	0.0
11	2.0075080802200228E-4	0.0	0.0	15.197639170497661	0.0
12	2.0075080802200228E-4	0.0	0.0	16.385883203179894	0.0
13	2.0075080802200228E-4	0.0	0.0	16.936542669584245	0.0
14	2.0075080802200228E-4	0.0	0.0	17.41031457651617	0.0
15	2.0075080802200228E-4	0.0	0.0	17.757814225202257	0.0
16	2.0075080802200228E-4	0.0	0.0	18.512838014173006	0.0
17	2.0075080802200228E-4	0.0	0.0	19.21787485194628	0.0
18	2.0075080802200228E-4	0.0	0.0	20.77690562704515	0.0
19	2.0075080802200228E-4	0.0	0.0	21.13785557986871	0.0
20	4.0150161604400456E-4	0.0	0.0	21.86397125248429	0.0
21	4.0150161604400456E-4	0.0	0.0	22.250617308734668	0.0
22	4.0150161604400456E-4	0.0	0.0	22.65272117720274	0.0
23	4.0150161604400456E-4	0.0	0.0	23.09015718788268	0.0
24	4.0150161604400456E-4	0.0	0.0	23.370003814265353	0.0
25	4.0150161604400456E-4	0.0	0.0	23.60829502338747	0.0
26	4.0150161604400456E-4	0.0	0.0	23.815269106458153	0.0
27	4.0150161604400456E-4	0.0	0.0	24.14369742838215	0.0
28	4.0150161604400456E-4	0.0	0.0	24.399855459418223	0.0
29	4.0150161604400456E-4	0.0	0.0	24.64095717985265	0.0
30	4.0150161604400456E-4	0.0	0.0	25.196033164033484	0.0
31	4.0150161604400456E-4	0.0	0.0	25.450986690221427	0.0
32	4.0150161604400456E-4	0.0	0.0	25.683255375102885	0.0
33	4.0150161604400456E-4	0.0	0.0	25.925762351193463	0.0
34	4.0150161604400456E-4	0.0	0.0	26.14096721739305	0.0
35	4.0150161604400456E-4	0.0	0.0	26.405155280750005	0.0
36	4.0150161604400456E-4	0.0	0.0	26.61915564210146	0.0
37	4.0150161604400456E-4	0.0	0.0	26.84098528496577	0.0
38	4.0150161604400456E-4	0.0	0.0	27.108184610443057	0.0
39	4.0150161604400456E-4	0.0	0.0	27.32901049926726	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.029432E-4	45.000004	1
CGTGGGA	30	2.1630149E-6	45.000004	4
CCTTATT	20	7.029432E-4	45.000004	18
GGTACGC	30	2.1630149E-6	45.000004	38
CTCGTTA	20	7.029432E-4	45.000004	11
TAAAGCG	30	2.1630149E-6	45.000004	1
ACGTTAG	20	7.029432E-4	45.000004	31
TTAGCCG	20	7.029432E-4	45.000004	15
AACCCGC	20	7.029432E-4	45.000004	43
ACCGCAA	20	7.029432E-4	45.000004	40
AGCTTCA	20	7.029432E-4	45.000004	12
TTCACGA	20	7.029432E-4	45.000004	21
TTGTCAC	20	7.029432E-4	45.000004	34
TGAACGA	20	7.029432E-4	45.000004	28
TGTATCC	20	7.029432E-4	45.000004	33
TTCGTAC	30	2.1630149E-6	45.000004	11
GATCAAT	20	7.029432E-4	45.000004	39
CGACATA	20	7.029432E-4	45.000004	14
CTAAGGT	20	7.029432E-4	45.000004	33
TATGCTA	20	7.029432E-4	45.000004	36
>>END_MODULE