FastQCFastQC Report
Sat 18 Jun 2016
SRR3548723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509485
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38840.76233843979705No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT34720.6814724672954062No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC16310.32012718725772105No Hit
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT16120.3163979312442957No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT16100.3160053779797246No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT14230.279301647742328No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT11820.23199897936151212No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9590.18822929036183597No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC7800.15309577318272374No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6720.13189789689588505No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT6600.12954257730845856No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC6270.12306544844303562No Hit
CCTAAAGGGATATAAGCACTCTCCAAGCAGTTGGGGTGTGAATCATACCTG5960.11698087284218377No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5810.11403672335790062No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5750.11285906356418736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATC253.8877366E-545.00000433
CAGGTAC253.8877366E-545.00000423
GTGCAAC253.8877366E-545.00000417
ATGGGCC502.1827873E-1145.0000045
GGTACGA253.8877366E-545.0000048
ACCGGAT253.8877366E-545.00000438
GATAACG253.8877366E-545.0000041
CTCAACT253.8877366E-545.00000429
GGTAACA253.8877366E-545.0000048
CAAGTAT253.8877366E-545.00000418
CATGACG253.8877366E-545.0000041
TATGGAT253.8877366E-545.00000427
TAGAACG253.8877366E-545.00000431
CCGGATG253.8877366E-545.00000439
ATAGCGC253.8877366E-545.00000410
ACTTCGT253.8877366E-545.00000410
ATGTACG253.8877366E-545.00000440
CGTTAGG502.1827873E-1145.0000042
GTAGCTA253.8877366E-545.00000413
TACGATG253.8877366E-545.0000041