##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548723_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509485
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084212489082113	31.0	31.0	34.0	28.0	34.0
2	31.301796912568573	31.0	31.0	34.0	28.0	34.0
3	31.293604325936975	31.0	31.0	34.0	28.0	34.0
4	35.34112289861331	37.0	35.0	37.0	33.0	37.0
5	34.99530506295573	35.0	35.0	37.0	32.0	37.0
6	35.02880752132055	36.0	35.0	37.0	32.0	37.0
7	34.96729246199594	36.0	35.0	37.0	32.0	37.0
8	35.33752318517719	37.0	35.0	37.0	33.0	37.0
9	35.720774900144264	39.0	35.0	39.0	31.0	39.0
10	35.60891095910576	37.0	35.0	39.0	30.0	39.0
11	36.050148679548954	38.0	35.0	39.0	31.0	39.0
12	35.74337811711827	37.0	35.0	39.0	31.0	39.0
13	35.37336133546621	37.0	35.0	39.0	30.0	39.0
14	36.33208043416391	38.0	35.0	40.0	31.0	41.0
15	36.47007272049226	38.0	35.0	40.0	31.0	41.0
16	36.52809013022954	38.0	35.0	40.0	31.0	41.0
17	36.45878092583688	38.0	35.0	40.0	31.0	41.0
18	36.35336663493528	38.0	35.0	40.0	31.0	41.0
19	36.3024348116235	38.0	35.0	40.0	31.0	41.0
20	36.22934728205934	38.0	34.0	40.0	30.0	41.0
21	36.156057587563915	38.0	34.0	40.0	30.0	41.0
22	36.12711070983444	38.0	34.0	40.0	30.0	41.0
23	36.039445714790425	38.0	34.0	40.0	30.0	41.0
24	35.70395988105636	37.0	34.0	40.0	29.0	41.0
25	35.763237386772914	37.0	34.0	40.0	30.0	41.0
26	35.65075517434272	37.0	34.0	40.0	29.0	41.0
27	35.52949154538406	37.0	34.0	40.0	29.0	41.0
28	35.4037959900684	37.0	34.0	40.0	28.0	41.0
29	35.116945543048374	37.0	34.0	40.0	27.0	41.0
30	35.15531566189387	37.0	34.0	40.0	27.0	41.0
31	34.988831859622955	37.0	34.0	40.0	27.0	41.0
32	34.9051650195786	37.0	34.0	40.0	26.0	41.0
33	34.708105243530234	37.0	33.0	40.0	25.0	41.0
34	34.63662521958448	37.0	33.0	40.0	25.0	41.0
35	34.53987457923197	37.0	33.0	40.0	25.0	41.0
36	34.20546434144283	36.0	33.0	40.0	24.0	41.0
37	34.125989970264094	36.0	33.0	40.0	24.0	41.0
38	34.031667271852946	36.0	33.0	40.0	23.0	41.0
39	33.90487256739649	36.0	33.0	40.0	23.0	41.0
40	33.71675711748138	36.0	33.0	39.0	23.0	41.0
41	33.73290479601951	36.0	33.0	39.0	23.0	41.0
42	33.66133448482291	35.0	33.0	39.0	23.0	41.0
43	33.58911449797344	35.0	33.0	39.0	23.0	41.0
44	33.56491162644631	35.0	33.0	39.0	23.0	41.0
45	33.34156648380227	35.0	32.0	39.0	22.0	40.0
46	33.16380462624022	35.0	32.0	39.0	21.0	40.0
47	33.046089678793294	35.0	32.0	39.0	21.0	40.0
48	32.91016025987026	35.0	31.0	39.0	20.0	40.0
49	32.78166776254453	35.0	31.0	39.0	20.0	40.0
50	32.61606524235257	35.0	31.0	39.0	20.0	40.0
51	30.79890673915817	34.0	28.0	37.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	9.0
11	9.0
12	12.0
13	10.0
14	18.0
15	64.0
16	145.0
17	298.0
18	657.0
19	1168.0
20	1901.0
21	2943.0
22	3929.0
23	5004.0
24	5729.0
25	6383.0
26	7338.0
27	8901.0
28	10431.0
29	12701.0
30	15798.0
31	20217.0
32	26118.0
33	35462.0
34	56007.0
35	59264.0
36	51107.0
37	63859.0
38	70550.0
39	43445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.311716733564285	33.482438148326246	20.164283541223	25.041561576886462
2	22.354534480897374	34.03044250566749	19.63139248456775	23.983630528867387
3	19.80980794331531	33.474783359667114	19.772907936445627	26.942500760571953
4	19.818836668400444	34.61554314651069	17.97364004828405	27.59198013680481
5	17.55674848130956	36.8366095174539	17.020324445273168	28.586317555963376
6	20.114625553254758	38.71458433516198	18.81390031109846	22.3568898004848
7	78.21800445547954	9.667016693327577	6.583510800121692	5.53146805107118
8	80.73819641402594	7.848906248466589	5.028214765891047	6.384682571616436
9	73.1829200074585	12.65670235630097	9.267986299891067	4.892391336349451
10	42.27700521114458	30.810916906287723	11.384437225826078	15.527640656741612
11	34.72349529426774	27.79159347183921	21.026526786853392	16.45838444703966
12	33.1858641569428	23.46781553922098	27.114242813821797	16.232077490014426
13	25.592313807079698	33.16368489749453	23.068981422416755	18.175019873009017
14	16.41461475803998	37.454488355888785	28.559035104075686	17.571861781995544
15	15.712533244354592	28.962187306790188	35.780052405860815	19.545227042994394
16	17.549878799179563	26.481839504597783	33.28164715349814	22.686634542724516
17	15.761798679058264	25.685152654150762	28.680726616092723	29.87232205069825
18	22.158846678508688	26.017448992610188	27.251243903157107	24.572460425724017
19	23.360648497993072	32.01703681168239	23.879603913756046	20.742710776568497
20	26.70539858877101	28.598094153900504	22.80184892587613	21.894658331452348
21	22.091720070267034	32.85435292501251	22.741395723132182	22.31253128158827
22	20.518170309233835	29.172007026703433	22.913334053014317	27.396488611048415
23	21.07598849818935	31.366772328920376	20.639076714721728	26.918162458168542
24	19.52599193303041	29.549839543853107	26.61530761455195	24.30886090856453
25	19.867709549839542	29.205962884088837	25.99173675378078	24.934590812290843
26	21.214363523950656	32.90695506246504	23.73828473851046	22.14039667507385
27	18.740885403888242	31.41486010383034	24.046831604463332	25.79742288781809
28	22.27484616818945	28.794763339450625	28.62832075527248	20.30206973708745
29	20.985504970705712	28.627928202007908	27.985907337801898	22.40065948948448
30	23.76713740345643	28.047930753604128	24.634680118158535	23.550251724780907
31	28.973571351462752	29.667409246592147	19.519318527532707	21.839700874412397
32	27.987084997595613	25.278663748687404	22.985367577063112	23.748883676653875
33	27.37587956465843	28.70369098207013	21.214167247318372	22.70626220595307
34	21.700737018754232	28.045771710648985	25.365025466893037	24.888465803703742
35	25.051571685133027	26.791171477079796	25.74501702699785	22.412239810789327
36	26.83670765577004	27.761563147099523	25.544226032169743	19.857503164960697
37	25.387793556238165	29.358666104006986	22.44776588123301	22.805774458521842
38	24.436636996182422	30.607181761975326	23.52767991206807	21.428501329774182
39	23.41697989145902	27.61592588594365	25.298683965180523	23.668410257416802
40	26.73366242382013	27.846943482143732	21.606720511889456	23.812673582146676
41	22.133526992943857	24.532419992737765	26.409413427284417	26.924639587033965
42	27.026899712454732	25.39093398235473	22.890565963669196	24.69160034152134
43	23.62974376085655	23.78166187424556	25.769159052768977	26.819435312128913
44	23.516688420660078	24.64233490681767	24.24369706664573	27.597279605876523
45	24.80799238446667	27.52053544265288	25.056282324307876	22.615189848572577
46	21.84382268369039	26.077705918721843	29.679185844529282	22.399285553058483
47	22.862890958516935	26.316378303581068	25.003091356958496	25.817639380943504
48	23.70589909418334	23.135322924129266	30.773035516256613	22.385742465430777
49	24.293747607878544	21.177856070345545	28.382778688283267	26.145617633492645
50	20.613168199260038	24.903186551125156	26.195864451357743	28.28778079825706
51	21.265002895080325	24.909859956622864	28.160593540536034	25.664543607760777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4525.0
1	3658.0
2	2791.0
3	2044.0
4	1297.0
5	1242.0
6	1187.0
7	1316.0
8	1445.0
9	1547.5
10	1650.0
11	1774.5
12	1899.0
13	1928.0
14	1957.0
15	1925.0
16	1893.0
17	1831.0
18	1769.0
19	1925.5
20	2082.0
21	1787.5
22	1493.0
23	1735.0
24	1977.0
25	1931.0
26	2399.5
27	2914.0
28	3319.0
29	3724.0
30	3964.0
31	4204.0
32	5328.0
33	6452.0
34	6688.0
35	6924.0
36	8402.0
37	9880.0
38	10060.5
39	10241.0
40	13477.5
41	16714.0
42	23543.5
43	30373.0
44	37368.0
45	44363.0
46	47271.5
47	50180.0
48	51241.5
49	52303.0
50	50641.5
51	48980.0
52	43949.5
53	38919.0
54	34289.0
55	29659.0
56	27118.5
57	24578.0
58	22274.5
59	19971.0
60	19357.0
61	18743.0
62	18447.0
63	18151.0
64	15781.5
65	13412.0
66	11321.0
67	9230.0
68	7710.0
69	6190.0
70	5610.0
71	5030.0
72	4585.0
73	4140.0
74	3362.5
75	1975.5
76	1366.0
77	1278.0
78	1190.0
79	873.5
80	557.0
81	424.5
82	292.0
83	192.0
84	92.0
85	141.0
86	190.0
87	114.5
88	39.0
89	38.5
90	38.0
91	23.0
92	8.0
93	4.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.896378498009152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70001860677124	24.62441941173176
2	8.966268741368498	5.540504654963785
3	3.307814736161183	3.065984882691846
4	1.5905702448708468	1.9657144125280313
5	0.9173914287246319	1.4172036406352804
6	0.5913315284649935	1.0962001632756426
7	0.4328061234105448	0.9360499263603775
8	0.3162756521426286	0.7817417806642666
9	0.2687262702481849	0.7472401702151594
>10	2.590748433578055	19.674634283023888
>50	0.947766852582289	21.12889092562026
>100	0.3606885997681586	15.017489534767636
>500	0.005116150351321399	1.0768554018799485
>1k	0.004476631557406224	2.9270708116421207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3884	0.76233843979705	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	3472	0.6814724672954062	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	1631	0.32012718725772105	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1612	0.3163979312442957	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1610	0.3160053779797246	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGT	1423	0.279301647742328	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	1182	0.23199897936151212	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	959	0.18822929036183597	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC	780	0.15309577318272374	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.13189789689588505	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	660	0.12954257730845856	No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTC	627	0.12306544844303562	No Hit
CCTAAAGGGATATAAGCACTCTCCAAGCAGTTGGGGTGTGAATCATACCTG	596	0.11698087284218377	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	581	0.11403672335790062	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	575	0.11285906356418736	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9295661305043328	0.0
2	0.0	0.0	0.0	1.1906140514441053	0.0
3	0.0	0.0	0.0	1.5380236905895168	0.0
4	0.0	0.0	0.0	3.530035231655495	0.0
5	0.0	0.0	0.0	3.875678381110337	0.0
6	0.0	0.0	0.0	5.645701051061366	0.0
7	0.0	0.0	0.0	7.007468325858465	0.0
8	0.0	0.0	0.0	8.12801161957663	0.0
9	0.0	0.0	0.0	9.644052327350167	0.0
10	0.0	0.0	0.0	11.876895296230508	0.0
11	0.0	0.0	0.0	14.03122761219663	0.0
12	0.0	0.0	0.0	15.113104409354545	0.0
13	0.0	0.0	0.0	15.650706105184648	0.0
14	1.9627663228554323E-4	0.0	0.0	16.114507787275386	0.0
15	1.9627663228554323E-4	0.0	0.0	16.43443869790082	0.0
16	1.9627663228554323E-4	0.0	0.0	17.17440160161732	0.0
17	1.9627663228554323E-4	0.0	0.0	17.846845343827592	0.0
18	1.9627663228554323E-4	0.0	0.0	19.320686575659735	0.0
19	1.9627663228554323E-4	0.0	0.0	19.659852596249152	0.0
20	1.9627663228554323E-4	0.0	0.0	20.36016762024397	0.0
21	1.9627663228554323E-4	0.0	0.0	20.73937407381964	0.0
22	1.9627663228554323E-4	0.0	0.0	21.12741297584816	0.0
23	1.9627663228554323E-4	0.0	0.0	21.562361992992923	0.0
24	1.9627663228554323E-4	0.0	0.0	21.830083319430404	0.0
25	3.9255326457108647E-4	0.0	0.0	22.061689745527346	0.0
26	3.9255326457108647E-4	0.0	0.0	22.259340314238887	0.0
27	3.9255326457108647E-4	0.0	0.0	22.592421759227456	0.0
28	3.9255326457108647E-4	0.0	0.0	22.840319145804095	0.0
29	3.9255326457108647E-4	0.0	0.0	23.069373975681327	0.0
30	3.9255326457108647E-4	0.0	0.0	23.621107589035987	0.0
31	5.888298968566298E-4	0.0	0.0	23.88352944640176	0.0
32	5.888298968566298E-4	0.0	0.0	24.108658743633278	0.0
33	5.888298968566298E-4	0.0	0.0	24.3269183587348	0.0
34	5.888298968566298E-4	0.0	0.0	24.52555031060777	0.0
35	5.888298968566298E-4	0.0	0.0	24.780317379314404	0.0
36	5.888298968566298E-4	0.0	0.0	24.990137099227653	0.0
37	5.888298968566298E-4	0.0	0.0	25.193872243540046	0.0
38	5.888298968566298E-4	0.0	0.0	25.456686654170387	0.0
39	5.888298968566298E-4	0.0	0.0	25.68338616446019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATC	25	3.8877366E-5	45.000004	33
CAGGTAC	25	3.8877366E-5	45.000004	23
GTGCAAC	25	3.8877366E-5	45.000004	17
ATGGGCC	50	2.1827873E-11	45.000004	5
GGTACGA	25	3.8877366E-5	45.000004	8
ACCGGAT	25	3.8877366E-5	45.000004	38
GATAACG	25	3.8877366E-5	45.000004	1
CTCAACT	25	3.8877366E-5	45.000004	29
GGTAACA	25	3.8877366E-5	45.000004	8
CAAGTAT	25	3.8877366E-5	45.000004	18
CATGACG	25	3.8877366E-5	45.000004	1
TATGGAT	25	3.8877366E-5	45.000004	27
TAGAACG	25	3.8877366E-5	45.000004	31
CCGGATG	25	3.8877366E-5	45.000004	39
ATAGCGC	25	3.8877366E-5	45.000004	10
ACTTCGT	25	3.8877366E-5	45.000004	10
ATGTACG	25	3.8877366E-5	45.000004	40
CGTTAGG	50	2.1827873E-11	45.000004	2
GTAGCTA	25	3.8877366E-5	45.000004	13
TACGATG	25	3.8877366E-5	45.000004	1
>>END_MODULE