FastQCFastQC Report
Sat 18 Jun 2016
SRR3548722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395201
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC103942.6300540737498133No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT89672.268971991467633No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT83232.1060169382162495No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT46421.174592169554227TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31210.7897247223564718No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT21000.5313751736458157No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT18370.4648267590416016No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG14780.37398690792786454No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT13320.33704368156963166No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT10380.26265115725921745No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT8990.22747918147980392No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7550.19104202671551943No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC7280.1842100601972161No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5930.1500502276056994No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT4790.12120414675064083No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC4440.1123478938565439TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTT253.8861457E-545.026
CTTCGAC207.027663E-445.034
TTCTAGT406.7957444E-945.013
AAGTAGA207.027663E-445.020
TTAGGCA207.027663E-445.027
AGACTAC207.027663E-445.028
CGGCGAA207.027663E-445.033
TCGTTGA551.8189894E-1245.024
CCCCTAG207.027663E-445.01
TACCCCC207.027663E-445.033
ACCGGCC207.027663E-445.026
ACACGAC700.045.026
TCCGCTT253.8861457E-545.028
TACCAGT253.8861457E-545.042
CGAAAAC207.027663E-445.020
GCGAAAA207.027663E-445.019
GCAACGC207.027663E-445.018
CGCATTG207.027663E-445.040
CAGTTGA253.8861457E-545.016
GGCGTGA207.027663E-445.08