##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395201
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16210232261558	31.0	31.0	34.0	28.0	34.0
2	31.32775220710474	31.0	31.0	34.0	28.0	34.0
3	31.494396522276006	31.0	31.0	34.0	30.0	34.0
4	35.3226206411421	37.0	35.0	37.0	33.0	37.0
5	34.877272577751576	35.0	35.0	37.0	32.0	37.0
6	34.86446137535077	35.0	35.0	37.0	32.0	37.0
7	34.761642809608276	35.0	35.0	37.0	32.0	37.0
8	35.146942948018854	37.0	35.0	37.0	32.0	37.0
9	36.038332392883625	39.0	35.0	39.0	32.0	39.0
10	35.788892234584424	37.0	35.0	39.0	30.0	39.0
11	36.077808507569564	37.0	35.0	39.0	31.0	39.0
12	35.556757194440294	37.0	35.0	39.0	30.0	39.0
13	35.2946399427127	37.0	35.0	39.0	30.0	39.0
14	36.20411385598721	38.0	35.0	40.0	30.0	41.0
15	36.355140801769224	38.0	35.0	40.0	31.0	41.0
16	36.29844054038325	38.0	35.0	40.0	31.0	41.0
17	36.27839251418898	38.0	35.0	40.0	31.0	41.0
18	36.02257585380604	38.0	35.0	40.0	30.0	41.0
19	35.9770698960782	38.0	34.0	40.0	30.0	41.0
20	36.082927421742355	38.0	34.0	40.0	30.0	41.0
21	36.124281062041845	38.0	34.0	40.0	30.0	41.0
22	36.0681222972614	38.0	34.0	40.0	30.0	41.0
23	36.0106705195584	38.0	34.0	40.0	30.0	41.0
24	35.59734160591699	37.0	34.0	40.0	29.0	41.0
25	35.7032775726782	37.0	34.0	40.0	30.0	41.0
26	35.61216697326171	37.0	34.0	40.0	29.0	41.0
27	35.51396631081399	37.0	34.0	40.0	29.0	41.0
28	35.40542913605988	37.0	34.0	40.0	29.0	41.0
29	35.21815734271927	37.0	34.0	40.0	27.0	41.0
30	35.201565785511676	37.0	34.0	40.0	27.0	41.0
31	34.852733166161016	37.0	33.0	40.0	26.0	41.0
32	34.92752042631471	37.0	34.0	40.0	27.0	41.0
33	34.934952087671846	37.0	34.0	40.0	27.0	41.0
34	34.81118468829786	37.0	34.0	40.0	26.0	41.0
35	34.753801230260045	37.0	33.0	40.0	26.0	41.0
36	34.462362190379075	37.0	33.0	40.0	24.0	41.0
37	34.30122140379199	37.0	33.0	40.0	24.0	41.0
38	34.18441501919276	36.0	33.0	40.0	24.0	41.0
39	34.16863823725142	36.0	33.0	40.0	24.0	41.0
40	34.01862596501527	36.0	33.0	40.0	23.0	41.0
41	34.08089554429265	36.0	33.0	40.0	23.0	41.0
42	33.974838120348885	36.0	33.0	40.0	23.0	41.0
43	33.835516610534896	36.0	33.0	39.0	23.0	41.0
44	33.67127360507691	36.0	33.0	39.0	23.0	40.0
45	33.203359809312225	35.0	32.0	39.0	22.0	40.0
46	33.10828160859917	35.0	32.0	39.0	21.0	40.0
47	32.98615641154754	35.0	31.0	39.0	20.0	40.0
48	32.96281385927667	35.0	31.0	39.0	20.0	40.0
49	32.81372010698354	35.0	31.0	39.0	20.0	40.0
50	32.448404735817974	35.0	31.0	39.0	18.0	40.0
51	30.256385990926137	34.0	26.0	37.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	7.0
12	8.0
13	12.0
14	16.0
15	27.0
16	92.0
17	210.0
18	414.0
19	809.0
20	1378.0
21	2034.0
22	2832.0
23	3450.0
24	4207.0
25	5041.0
26	5543.0
27	6706.0
28	8197.0
29	10432.0
30	12816.0
31	16465.0
32	21004.0
33	27999.0
34	43654.0
35	47034.0
36	39807.0
37	48177.0
38	53827.0
39	32993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.028294462817655	40.637295958259216	18.303344374128606	23.031065204794523
2	19.13051839443726	40.93942069984641	19.457946715721874	20.472114189994457
3	18.585985359348786	31.178564831566725	28.33925015371925	21.896199655365244
4	24.682882887442087	30.44627923512339	18.116856991758624	26.753980885675897
5	15.561954549710148	40.186639203848166	19.118625711979472	25.132780534462213
6	20.01108296790747	35.08746182322413	25.669722495641455	19.23173271322694
7	68.94112110040207	14.85573164035516	9.330948049220524	6.872199210022242
8	71.05599429151242	7.317289176899856	7.010862826764103	14.615853704823623
9	68.09598153850825	11.497187507116632	13.047537835177542	7.359293119197573
10	41.130715762358896	26.862280206780852	14.188476243734202	17.818527787126044
11	33.464743257228605	23.86532422741845	23.778532949056302	18.891399566296645
12	30.656551982408953	22.36001427121895	31.956396871465408	15.027036874906694
13	24.32559634211452	34.622635064182525	22.185672607103726	18.866095986599223
14	14.894446117292212	34.83973977798639	33.77648335910081	16.489330745620585
15	12.930382261178488	29.004734299761388	34.098850964445944	23.96603247461418
16	13.833213984782427	26.05787940819988	37.30000683196652	22.808899775051174
17	14.124964258693678	26.33368842690175	28.30812675069142	31.23322056371315
18	18.335226884547357	26.258030723606467	28.14112312468845	27.265619267157724
19	20.712245161322972	30.756248086416786	28.945523923269423	19.585982828990815
20	24.19907844362742	31.18944537083661	23.570284488146537	21.04119169738943
21	17.95213068792842	31.278767007168508	24.661627880496255	26.107474424406824
22	17.880774593181698	32.18387605294521	24.895179921103438	25.040169432769655
23	21.117102436481687	29.89820369887728	18.75248291375781	30.23221095088322
24	15.333463225042445	35.08062985670583	25.608740868570678	23.97716604968105
25	18.95440547974322	26.013345107932416	27.371388230292936	27.66086118203142
26	20.857740744583136	33.47182825954388	20.538156533004724	25.13227446286826
27	16.185687789251546	28.778014225672504	27.88150839699292	27.154789588083027
28	24.24310667230093	26.472099007846637	26.821035371874057	22.46375894797837
29	17.383306216330425	28.298005318812454	29.137578093172838	25.181110371684284
30	22.672766516279058	27.256256942669683	25.805602718616605	24.26537382243466
31	26.764608389148815	32.33088985098722	18.60597518730975	22.29852657255422
32	24.765625593052647	23.91213584985868	24.466031209435197	26.85620734765347
33	27.27017391150326	25.70287018504508	21.227173008165465	25.799782895286192
34	18.95086297858558	24.341537597323892	27.10443546448516	29.603163959605368
35	26.0194179670598	22.198324396952437	28.10544507731509	23.676812558672676
36	22.824587994463577	24.021447314151533	30.163385214106242	22.99057947727865
37	20.201112851435095	27.301297314531087	28.44932072540302	24.048269108630798
38	23.006520732488024	28.604684704745182	25.359247572754118	23.02954699001268
39	19.531327096844393	23.56800716597377	33.648953317425814	23.251712419756025
40	26.081411737318476	23.850901186990924	23.274232605686727	26.79345447000387
41	20.093825673518033	18.61154197484318	34.9151444454847	26.379487906154083
42	29.86556208106761	21.155563877621766	23.087239151722795	25.891634889587827
43	22.47944716739077	21.622667958836136	27.699069587374524	28.198815286398567
44	21.567759190892737	21.13405583487896	27.563948471790304	29.734236502437998
45	26.41541898932442	27.083686529133278	23.92251031753462	22.57838416400768
46	19.960222772715657	23.21122669224015	34.751177249045426	22.077373285998767
47	22.461734661602577	24.496648540869074	24.31218544487489	28.72943135265346
48	21.334460186082524	19.002988352762266	36.145657526170226	23.516893934984985
49	24.73728558379154	17.974144802265176	30.73119754251634	26.557372071426943
50	20.617356737457648	21.7524753226839	26.1684560514776	31.46171188838085
51	19.021459965941382	22.09559186338091	32.01763153433316	26.865316636344545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3618.0
1	2876.5
2	2135.0
3	1453.5
4	772.0
5	797.5
6	823.0
7	868.0
8	913.0
9	1005.5
10	1098.0
11	1151.0
12	1204.0
13	1289.0
14	1374.0
15	1360.5
16	1347.0
17	1353.0
18	1359.0
19	1330.0
20	1301.0
21	1307.5
22	1314.0
23	1306.5
24	1299.0
25	1239.0
26	1415.0
27	1651.0
28	2249.5
29	2848.0
30	3214.5
31	3581.0
32	3836.5
33	4092.0
34	4545.0
35	4998.0
36	5578.5
37	6159.0
38	7093.5
39	8028.0
40	10044.0
41	12060.0
42	18261.0
43	24462.0
44	40602.5
45	56743.0
46	53925.0
47	51107.0
48	47209.0
49	43311.0
50	39050.5
51	34790.0
52	30592.0
53	26394.0
54	23133.0
55	19872.0
56	17521.0
57	15170.0
58	14729.0
59	14288.0
60	12324.0
61	10360.0
62	9985.5
63	9611.0
64	8421.0
65	7231.0
66	6360.5
67	5490.0
68	4905.0
69	4320.0
70	3584.5
71	2849.0
72	2443.0
73	2037.0
74	1770.5
75	1214.5
76	925.0
77	821.0
78	717.0
79	562.5
80	408.0
81	312.0
82	216.0
83	171.0
84	126.0
85	77.0
86	28.0
87	47.0
88	66.0
89	40.0
90	14.0
91	7.5
92	1.0
93	3.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.351218093784244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60738452738074	25.271396922874427
2	8.727999192995524	5.472668124439511
3	3.0410141415961394	2.860184927383879
4	1.3947607355871738	1.7490979204016173
5	0.8034709281021261	1.2594896149472496
6	0.5458406203277895	1.0267660999405803
7	0.3442313317761376	0.7554450090059244
8	0.3087510939112074	0.7743778305523779
9	0.246676719476849	0.6960254067880135
>10	2.898848538076954	22.114303541690035
>50	0.9343062609408573	20.09522716347936
>100	0.13543007061092668	6.247029693969248
>500	0.0032245254907363493	0.7518777141984996
>1k	0.005642919608788611	3.929477210204461
>5k	0.0016122627453681747	4.3697363625183385
>10k+	8.061313726840873E-4	2.6268964576064553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	10394	2.6300540737498133	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	8967	2.268971991467633	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	8323	2.1060169382162495	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	4642	1.174592169554227	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3121	0.7897247223564718	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	2100	0.5313751736458157	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1837	0.4648267590416016	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1478	0.37398690792786454	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1332	0.33704368156963166	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	1038	0.26265115725921745	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	899	0.22747918147980392	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	755	0.19104202671551943	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	728	0.1842100601972161	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	593	0.1500502276056994	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	479	0.12120414675064083	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	444	0.1123478938565439	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.591073909225939E-4	0.0	0.0	1.607030346583131	0.0
2	7.591073909225939E-4	0.0	0.0	1.9658351066925437	0.0
3	7.591073909225939E-4	0.0	0.0	2.8269159237957395	0.0
4	7.591073909225939E-4	0.0	0.0	7.395477238164883	0.0
5	7.591073909225939E-4	0.0	0.0	7.937732951080589	0.0
6	7.591073909225939E-4	0.0	0.0	10.2922310419255	0.0
7	7.591073909225939E-4	0.0	0.0	12.234786855296418	0.0
8	7.591073909225939E-4	0.0	0.0	13.489085300897518	0.0
9	7.591073909225939E-4	0.0	0.0	17.31549262274134	0.0
10	7.591073909225939E-4	0.0	0.0	19.65303731518898	0.0
11	7.591073909225939E-4	0.0	0.0	24.52043390578465	0.0
12	7.591073909225939E-4	0.0	0.0	25.739560375606338	0.0
13	7.591073909225939E-4	0.0	0.0	26.39542916136346	0.0
14	7.591073909225939E-4	0.0	0.0	27.17553852343491	0.0
15	7.591073909225939E-4	0.0	0.0	27.600385626554587	0.0
16	7.591073909225939E-4	0.0	0.0	28.271689595927135	0.0
17	7.591073909225939E-4	0.0	0.0	28.974370004124484	0.0
18	7.591073909225939E-4	0.0	0.0	30.26409346130197	0.0
19	0.0010121431878967917	0.0	0.0	30.649466980093674	0.0
20	0.0010121431878967917	0.0	0.0	31.106955701023022	0.0
21	0.0010121431878967917	0.0	0.0	31.526489052406244	0.0
22	0.0010121431878967917	0.0	0.0	31.88858327787632	0.0
23	0.0010121431878967917	0.0	0.0	32.25295482551917	0.0
24	0.0010121431878967917	0.0	0.0	32.51889544813905	0.0
25	0.0010121431878967917	0.0	0.0	32.727396944845786	0.0
26	0.0010121431878967917	0.0	0.0	32.93210290459791	0.0
27	0.0010121431878967917	0.0	0.0	33.191464596496466	0.0
28	0.0010121431878967917	0.0	0.0	33.39870091421833	0.0
29	0.0010121431878967917	0.0	0.0	33.62187848714958	0.0
30	0.0010121431878967917	0.0	0.0	34.09125989053671	0.0
31	0.0010121431878967917	0.0	0.0	34.311907105498214	0.0
32	0.0010121431878967917	0.0	0.0	34.52142074539285	0.0
33	0.0010121431878967917	0.0	0.0	34.73523599383605	0.0
34	0.0010121431878967917	0.0	0.0	34.92855534272434	0.0
35	0.0010121431878967917	0.0	0.0	35.18437453346525	0.0
36	0.0010121431878967917	0.0	0.0	35.35340244584401	0.0
37	0.0010121431878967917	0.0	0.0	35.528503217350156	0.0
38	0.0010121431878967917	0.0	0.0	35.71043595537461	0.0
39	0.0012651789848709897	0.0	0.0	35.91337066454791	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTT	25	3.8861457E-5	45.0	26
CTTCGAC	20	7.027663E-4	45.0	34
TTCTAGT	40	6.7957444E-9	45.0	13
AAGTAGA	20	7.027663E-4	45.0	20
TTAGGCA	20	7.027663E-4	45.0	27
AGACTAC	20	7.027663E-4	45.0	28
CGGCGAA	20	7.027663E-4	45.0	33
TCGTTGA	55	1.8189894E-12	45.0	24
CCCCTAG	20	7.027663E-4	45.0	1
TACCCCC	20	7.027663E-4	45.0	33
ACCGGCC	20	7.027663E-4	45.0	26
ACACGAC	70	0.0	45.0	26
TCCGCTT	25	3.8861457E-5	45.0	28
TACCAGT	25	3.8861457E-5	45.0	42
CGAAAAC	20	7.027663E-4	45.0	20
GCGAAAA	20	7.027663E-4	45.0	19
GCAACGC	20	7.027663E-4	45.0	18
CGCATTG	20	7.027663E-4	45.0	40
CAGTTGA	25	3.8861457E-5	45.0	16
GGCGTGA	20	7.027663E-4	45.0	8
>>END_MODULE