SRR3548721_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399788
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	9631	2.4090267841956234	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	8252	2.064093969803996	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGT	8151	2.03883058020751	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	4564	1.1416050506768587	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3304	0.8264380121464376	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	2046	0.511771238756541	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1576	0.39420893073328866	No Hit
AACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1402	0.35068586350765907	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1168	0.29215484206629516	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT	1051	0.2628893313456132	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	870	0.21761533612814793	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	851	0.2128628172931654	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	711	0.1778442574564519	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	620	0.15508219356258818	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	472	0.1180625731637768	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	406	0.10155382352646902	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATG	30	2.1619362E-6	45.000004	19
CGGCGAA	30	2.1619362E-6	45.000004	33
CTTGACC	30	2.1619362E-6	45.000004	27
AATCTAG	30	2.1619362E-6	45.000004	1
ACTCGCT	30	2.1619362E-6	45.000004	10
ACGTACG	30	2.1619362E-6	45.000004	1
GTAACGG	65	0.0	45.000004	2
GGCTAGA	30	2.1619362E-6	45.000004	14
AACGTAC	20	7.02776E-4	45.0	32
GAAATAC	25	3.8862254E-5	45.0	39
AGACTAC	20	7.02776E-4	45.0	28
CAGCGCA	20	7.02776E-4	45.0	34
CCTTAGC	25	3.8862254E-5	45.0	42
CGTATGG	25	3.8862254E-5	45.0	2
CTCGTCA	25	3.8862254E-5	45.0	28
GATCGAT	25	3.8862254E-5	45.0	12
GATCGAA	20	7.02776E-4	45.0	24
CTTCCGT	20	7.02776E-4	45.0	41
CAAAACG	25	3.8862254E-5	45.0	1
GCGAAAA	20	7.02776E-4	45.0	19