##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526854
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178017059754694	31.0	31.0	34.0	28.0	34.0
2	31.388963545877985	31.0	31.0	34.0	28.0	34.0
3	31.357493726914857	31.0	31.0	34.0	28.0	34.0
4	35.382982761827755	37.0	35.0	37.0	33.0	37.0
5	35.04835495222586	37.0	35.0	37.0	32.0	37.0
6	35.107507962357694	36.0	35.0	37.0	32.0	37.0
7	35.04992085093783	37.0	35.0	37.0	32.0	37.0
8	35.41170988547112	37.0	35.0	37.0	33.0	37.0
9	35.76546063994959	39.0	37.0	39.0	32.0	39.0
10	35.622772153196145	38.0	35.0	39.0	30.0	39.0
11	36.08647367202299	38.0	35.0	39.0	31.0	39.0
12	35.9211527292191	37.0	35.0	39.0	32.0	39.0
13	35.63752197003345	37.0	35.0	39.0	30.0	39.0
14	36.68020741989242	38.0	35.0	40.0	31.0	41.0
15	36.76034726888284	38.0	35.0	40.0	31.0	41.0
16	36.81039339171763	38.0	35.0	40.0	31.0	41.0
17	36.73653042398843	38.0	35.0	40.0	31.0	41.0
18	36.63161141416787	38.0	35.0	40.0	31.0	41.0
19	36.63022203494706	38.0	35.0	40.0	31.0	41.0
20	36.56005838429622	38.0	35.0	40.0	31.0	41.0
21	36.538929191009274	38.0	35.0	40.0	31.0	41.0
22	36.49748696982466	38.0	35.0	40.0	31.0	41.0
23	36.4569197538597	38.0	35.0	40.0	31.0	41.0
24	36.10017196414946	38.0	34.0	40.0	30.0	41.0
25	36.12991644744085	38.0	35.0	40.0	30.0	41.0
26	36.08201133520861	38.0	35.0	40.0	30.0	41.0
27	35.964200708355634	38.0	34.0	40.0	30.0	41.0
28	35.72636441974437	38.0	34.0	40.0	29.0	41.0
29	35.39292479510453	38.0	34.0	40.0	27.0	41.0
30	35.36813234786108	38.0	34.0	40.0	27.0	41.0
31	35.29959912992973	38.0	34.0	40.0	27.0	41.0
32	35.23372699077923	38.0	34.0	40.0	27.0	41.0
33	35.15923956162428	37.0	34.0	40.0	27.0	41.0
34	35.09752606984098	37.0	34.0	40.0	27.0	41.0
35	35.00013666025123	37.0	34.0	40.0	26.0	41.0
36	34.798276182775496	37.0	33.0	40.0	26.0	41.0
37	34.686522262334535	37.0	33.0	40.0	25.0	41.0
38	34.53939041935717	37.0	33.0	40.0	25.0	41.0
39	34.36670880357746	37.0	33.0	40.0	24.0	41.0
40	34.19850850520258	37.0	33.0	40.0	23.0	41.0
41	34.168371123688914	36.0	33.0	40.0	23.0	41.0
42	34.07515744399777	36.0	33.0	40.0	23.0	41.0
43	33.893530275939824	36.0	33.0	40.0	23.0	41.0
44	33.785397852156386	36.0	33.0	40.0	23.0	41.0
45	33.4463494630391	35.0	32.0	39.0	22.0	41.0
46	33.18067624047649	35.0	32.0	39.0	20.0	41.0
47	33.05340189122603	35.0	31.0	39.0	20.0	40.0
48	32.949471770167825	35.0	31.0	39.0	20.0	40.0
49	32.73114942659636	35.0	31.0	39.0	18.0	40.0
50	32.57165362700103	35.0	31.0	39.0	18.0	40.0
51	30.714810934338544	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	7.0
11	7.0
12	13.0
13	22.0
14	40.0
15	71.0
16	124.0
17	265.0
18	530.0
19	935.0
20	1526.0
21	2408.0
22	3317.0
23	4407.0
24	5233.0
25	6082.0
26	7267.0
27	8742.0
28	10766.0
29	13397.0
30	16361.0
31	20495.0
32	25933.0
33	34314.0
34	52960.0
35	56428.0
36	53987.0
37	68804.0
38	79264.0
39	53142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.013601491115185	31.94812984242314	20.738382929616176	26.299885736845503
2	24.125659101003315	31.934653623204913	19.766956310476907	24.17273096531487
3	21.36739969706978	31.618816598146736	20.650882407649938	26.36290129713355
4	20.380219187858494	33.65087861153185	19.638267907238056	26.330634293371595
5	18.210927505532844	35.74064161987951	17.69408602762815	28.3543448469595
6	20.328022564125924	38.43550585171604	19.327365835696416	21.909105748461624
7	81.45007914906218	7.986463042892339	5.81679174875772	4.746666059287772
8	82.79770107088491	6.401204128658035	4.927930698068155	5.8731641023888965
9	74.31071985787334	12.418620718453308	8.461167610002011	4.80949181367134
10	41.65784069210825	29.905059086578067	12.305306593477509	16.131793627836174
11	32.747212700292685	27.88381600974843	21.942321781745985	17.4266495082129
12	31.32214996944125	23.15195481101026	27.71356011342801	17.81233510612048
13	25.036347830708316	31.464884009611772	23.487911261943538	20.010856897736375
14	19.55968826278248	32.87134576182396	27.868821343294346	19.700144632099214
15	17.37255482543551	27.463395931320633	34.017014201277775	21.147035041966085
16	18.800085033045207	25.175475558693677	32.04113473561935	23.983304672641754
17	18.04522695091999	25.32807950589727	27.104283160040545	29.5224103831422
18	23.34384858044164	24.967258481476843	26.597501395073397	25.09139154300812
19	24.34754220334286	30.21994708211383	24.3471625915339	21.08534812300941
20	26.66697035611384	27.881348532990163	22.479852103239228	22.97182900765677
21	23.693471056497625	29.99863339748773	22.827007102536946	23.4808884434777
22	21.529114327688507	28.386991462530414	22.620118666651482	27.4637755431296
23	21.611490090233726	29.497735615559527	21.283315681384217	27.607458612822526
24	19.647758202462164	28.379589032255616	26.419653262573696	25.55299950270853
25	20.734966423335496	28.681190614477636	24.47698983019964	26.10685313198723
26	21.88139408640724	29.79117554388882	23.05951933552749	25.26791103417645
27	19.976312223120637	29.359746723001056	23.900169686478606	26.7637713673997
28	22.652575476317917	28.61286048886409	27.28573760472541	21.448826430092588
29	21.170001556408415	28.461015765278425	27.870719402339166	22.49826327597399
30	24.167036788180408	30.20628105699112	24.043283338458092	21.58339881637038
31	27.33755461664901	29.823822159459734	19.679076176701706	23.159547047189545
32	25.35446252662028	30.06734313491024	21.588143963982432	22.99005037448705
33	26.703033477965434	27.739563522342053	22.78202310317469	22.775379896517823
34	21.697282359059624	29.883990631180556	23.270773307216043	25.14795370254378
35	22.64555265785208	29.199360733713704	24.79871083829676	23.356375770137458
36	25.989173471208343	28.218254013445847	24.653888933176933	21.138683582168873
37	23.660444829117743	30.910081350810657	21.412383696432027	24.017090123639566
38	24.442634961488384	28.365543395323943	22.305230671115716	24.88659097207196
39	24.677994283046157	26.051429807878463	23.297346133843533	25.97322977523185
40	26.399723642603075	25.07810512969437	21.64204884085534	26.880122386847212
41	20.842586371176836	22.68294442103505	26.1442448951702	30.330224312617915
42	25.803922908433837	24.16722659408489	23.171504819171915	26.857345678309365
43	22.338067092591114	25.244754713829636	26.195682295284843	26.221495898294407
44	23.212692700444528	25.367179522220578	23.92579348358369	27.49433429375121
45	24.549685491616273	26.834189357962547	24.466740311357608	24.149384839063572
46	23.23603882669582	26.063956997574277	27.02266662111325	23.67733755461665
47	21.97838490359758	26.407315878782356	24.520455382326034	27.09384383529403
48	23.71321087056376	22.4918098752216	28.07020540794983	25.72477384626481
49	23.64279288000091	23.406674334825208	26.84273062366424	26.107802161509643
50	21.80907803679957	23.896942986102413	25.78323406484529	28.51074491225273
51	22.044247552452862	25.096516302429134	26.28621971172279	26.573016433395207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2906.0
1	2368.5
2	1831.0
3	1309.0
4	787.0
5	799.0
6	811.0
7	856.5
8	902.0
9	1023.0
10	1144.0
11	1208.5
12	1273.0
13	1313.0
14	1353.0
15	1370.0
16	1387.0
17	1401.5
18	1416.0
19	1379.5
20	1343.0
21	1329.5
22	1316.0
23	1430.0
24	1544.0
25	1836.5
26	2435.5
27	2742.0
28	3731.5
29	4721.0
30	5035.5
31	5350.0
32	6484.0
33	7618.0
34	8827.0
35	10036.0
36	10668.0
37	11300.0
38	12259.5
39	13219.0
40	15179.0
41	17139.0
42	23364.5
43	29590.0
44	35672.5
45	41755.0
46	45067.5
47	48380.0
48	49213.5
49	50047.0
50	48393.5
51	46740.0
52	42947.5
53	39155.0
54	34540.0
55	29925.0
56	28215.5
57	26506.0
58	25493.0
59	24480.0
60	22654.0
61	20828.0
62	20465.5
63	20103.0
64	18315.5
65	16528.0
66	13340.0
67	10152.0
68	9215.0
69	8278.0
70	7446.0
71	6614.0
72	6110.0
73	5606.0
74	4649.5
75	3133.5
76	2574.0
77	1964.5
78	1355.0
79	1215.0
80	1075.0
81	803.0
82	531.0
83	429.0
84	327.0
85	237.5
86	148.0
87	126.0
88	104.0
89	84.5
90	65.0
91	41.0
92	17.0
93	11.0
94	5.0
95	5.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.27100927489312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71927869888624	26.856118702533266
2	8.48436946783233	5.645670705117389
3	2.964277719329255	2.95873534479488
4	1.3610550420933767	1.811346997165151
5	0.8323268163472952	1.3846176613216556
6	0.53502794332676	1.0680551798850972
7	0.3393332886634626	0.7902972696081433
8	0.30757551843614506	0.8186678341295233
9	0.21116748373722172	0.632317977897925
>10	3.073725996489254	25.46880224723384
>50	1.0263815975301818	23.636195660049744
>100	0.13860733627360713	6.329524475938471
>500	0.0040093031153522725	0.8586734092602362
>1k	0.0028637879395373377	1.7409765350646957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2484	0.4714778667334783	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	2343	0.44471523420150555	TruSeq Adapter, Index 15 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	1572	0.2983748818458245	No Hit
AATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	1567	0.2974258523234141	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGT	1170	0.22207290824402967	No Hit
AAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	884	0.16778841956215573	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	773	0.14671996416464522	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	639	0.12128597296404697	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	604	0.1146427663071743	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	564	0.10705053012789122	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	537	0.10192577070687514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7258177787394611	0.0
2	0.0	0.0	0.0	0.9556727290672559	0.0
3	0.0	0.0	0.0	1.2739772308836983	0.0
4	0.0	0.0	0.0	2.9243395703553547	0.0
5	0.0	0.0	0.0	3.255171261867614	0.0
6	0.0	0.0	0.0	4.65138349523777	0.0
7	0.0	0.0	0.0	5.821536896369772	0.0
8	0.0	0.0	0.0	6.946896104044004	0.0
9	0.0	0.0	0.0	8.321850076112169	0.0
10	0.0	0.0	0.0	10.307979060612617	0.0
11	0.0	0.0	0.0	12.172062848531093	0.0
12	0.0	0.0	0.0	13.113120522953228	0.0
13	0.0	0.0	0.0	13.631860059902744	0.0
14	0.0	0.0	0.0	14.059682568605345	0.0
15	0.0	0.0	0.0	14.32617005849818	0.0
16	0.0	0.0	0.0	14.844719789543213	0.0
17	0.0	0.0	0.0	15.429511781252492	0.0
18	0.0	0.0	0.0	16.29502670569076	0.0
19	0.0	0.0	0.0	16.605549165423437	0.0
20	0.0	0.0	0.0	16.96314348946767	0.0
21	0.0	0.0	0.0	17.30403489391748	0.0
22	0.0	0.0	0.0	17.654416593591392	0.0
23	0.0	0.0	0.0	18.00954344087736	0.0
24	7.592236179283066E-4	0.0	0.0	18.24926829823822	1.8980590448207664E-4
25	7.592236179283066E-4	0.0	0.0	18.46944314743743	1.8980590448207664E-4
26	7.592236179283066E-4	0.0	0.0	18.66589225857638	1.8980590448207664E-4
27	7.592236179283066E-4	0.0	0.0	18.912639934403078	1.8980590448207664E-4
28	7.592236179283066E-4	0.0	0.0	19.131486142270912	1.8980590448207664E-4
29	7.592236179283066E-4	0.0	0.0	19.362859539834567	1.8980590448207664E-4
30	7.592236179283066E-4	0.0	0.0	19.760313103820035	1.8980590448207664E-4
31	7.592236179283066E-4	0.0	0.0	19.9947233958554	1.8980590448207664E-4
32	7.592236179283066E-4	0.0	0.0	20.24450796615381	1.8980590448207664E-4
33	7.592236179283066E-4	0.0	0.0	20.500745937204613	1.8980590448207664E-4
34	7.592236179283066E-4	0.0	0.0	20.775584886894663	1.8980590448207664E-4
35	7.592236179283066E-4	0.0	0.0	21.051183060202636	1.8980590448207664E-4
36	7.592236179283066E-4	0.0	0.0	21.271357909401846	1.8980590448207664E-4
37	7.592236179283066E-4	0.0	0.0	21.48545896965763	1.8980590448207664E-4
38	7.592236179283066E-4	0.0	0.0	21.700698865340303	1.8980590448207664E-4
39	7.592236179283066E-4	0.0	0.0	21.966996549328655	1.8980590448207664E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCA	20	7.0298027E-4	45.000004	9
GATCGTG	40	6.8030204E-9	45.000004	29
TTATGGG	160	0.0	45.000004	3
GAATCGA	20	7.0298027E-4	45.000004	25
GTGATCG	20	7.0298027E-4	45.000004	8
CGTCTAA	20	7.0298027E-4	45.000004	12
TCGATAC	20	7.0298027E-4	45.000004	11
ATCGTGA	40	6.8030204E-9	45.000004	30
ATCGTAG	20	7.0298027E-4	45.000004	1
TAACCTA	20	7.0298027E-4	45.000004	33
TCGTGAG	40	6.8030204E-9	45.000004	31
GTTAAGT	20	7.0298027E-4	45.000004	29
CCATCCC	20	7.0298027E-4	45.000004	33
TGATTCG	20	7.0298027E-4	45.000004	17
TACGAGT	20	7.0298027E-4	45.000004	43
ACGTGAC	20	7.0298027E-4	45.000004	12
TATTAGG	40	6.8030204E-9	45.000004	2
TTGCGCA	20	7.0298027E-4	45.000004	29
AATTACG	20	7.0298027E-4	45.000004	1
CAATGTG	20	7.0298027E-4	45.000004	22
>>END_MODULE