SRR3548718_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585725
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	7981	1.362584830765291	TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6059	1.0344444918690512	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	5524	0.943104699304281	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5254	0.897007981561313	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5071	0.8657646506466344	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2995	0.5113321097784796	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2170	0.37048102778607706	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1458	0.24892227581202786	TruSeq Adapter, Index 19 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1406	0.24004438943190065	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	1318	0.22502027401937771	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1288	0.21989841649238123	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1257	0.21460583038115155	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1186	0.2024841009005933	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	961	0.16407016944811986	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	933	0.15928976908958983	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	915	0.15621665457339196	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	718	0.12258312347944854	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTTACCCTC	703	0.12002219471595033	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	662	0.1130223227623885	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	648	0.11063212258312348	TruSeq Adapter, Index 19 (95% over 22bp)
TACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	623	0.1063639079772931	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	609	0.10397370779802809	No Hit
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	609	0.10397370779802809	TruSeq Adapter, Index 19 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.10380297921379487	No Hit
CACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	601	0.10260787912416236	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	599	0.10226642195569594	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8048394E-9	45.000004	1
CGGAACG	40	6.8048394E-9	45.000004	1
GAGTGAC	20	7.0304493E-4	45.000004	9
AATTTGC	25	3.8884536E-5	45.000004	11
ATCTACG	25	3.8884536E-5	45.000004	1
CTCCGCG	20	7.0304493E-4	45.000004	1
CGAAACG	25	3.8884536E-5	45.000004	1
ACGCATG	20	7.0304493E-4	45.000004	1
GACGTCT	20	7.0304493E-4	45.000004	18
CGACCGG	20	7.0304493E-4	45.000004	2
TTTCCGG	50	2.1827873E-11	45.000004	2
ACGGGCC	40	6.8048394E-9	45.000004	5
TATTGCG	20	7.0304493E-4	45.000004	1
GCTCTCG	20	7.0304493E-4	45.000004	1
GTTGTAC	25	3.8884536E-5	45.000004	36
CGCTAAG	65	0.0	45.000004	1
TGACACG	25	3.8884536E-5	45.000004	1
GTCACGA	20	7.0304493E-4	45.000004	11
ACCCGTT	20	7.0304493E-4	45.000004	14
GCAATCG	20	7.0304493E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content