##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585725
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.850284689914208	31.0	30.0	33.0	28.0	34.0
2	31.12260019633787	31.0	31.0	34.0	28.0	34.0
3	31.339020871569424	31.0	31.0	34.0	30.0	34.0
4	35.35156430065304	37.0	35.0	37.0	33.0	37.0
5	34.59535959708054	35.0	35.0	37.0	32.0	37.0
6	34.69689530069572	35.0	35.0	37.0	32.0	37.0
7	34.727734004865766	35.0	35.0	37.0	32.0	37.0
8	35.108325579410135	35.0	35.0	37.0	32.0	37.0
9	36.41878697340902	38.0	35.0	39.0	32.0	39.0
10	35.76545990012378	37.0	35.0	39.0	30.0	39.0
11	36.148619232575015	37.0	35.0	39.0	32.0	39.0
12	36.120619744760766	37.0	35.0	39.0	32.0	39.0
13	35.83510521149003	37.0	35.0	39.0	30.0	39.0
14	36.73838917580776	38.0	35.0	40.0	31.0	41.0
15	36.79951683810662	38.0	35.0	40.0	32.0	41.0
16	36.591023091041016	38.0	35.0	40.0	31.0	41.0
17	36.53298049425925	38.0	35.0	40.0	31.0	41.0
18	36.42203764565282	38.0	35.0	40.0	31.0	41.0
19	36.45230440906569	38.0	35.0	40.0	31.0	41.0
20	36.40153314268641	38.0	35.0	40.0	30.0	41.0
21	36.47275598617099	38.0	35.0	40.0	31.0	41.0
22	36.361374365103075	38.0	35.0	40.0	30.0	41.0
23	36.33202782875923	38.0	35.0	40.0	30.0	41.0
24	35.89146783900294	38.0	34.0	40.0	29.0	41.0
25	36.01921208758376	38.0	34.0	40.0	30.0	41.0
26	36.02672755986171	38.0	34.0	40.0	30.0	41.0
27	35.91052114900337	38.0	34.0	40.0	30.0	41.0
28	35.84975372401724	38.0	34.0	40.0	29.0	41.0
29	35.106958043450426	38.0	34.0	40.0	26.0	41.0
30	35.29811771735883	38.0	34.0	40.0	27.0	41.0
31	35.16899056724572	38.0	34.0	40.0	27.0	41.0
32	35.02085620384993	38.0	34.0	40.0	26.0	41.0
33	35.17349780186948	38.0	34.0	40.0	27.0	41.0
34	35.110135302403	38.0	34.0	40.0	26.0	41.0
35	35.09860941568142	38.0	34.0	40.0	26.0	41.0
36	35.156334457296516	38.0	34.0	40.0	27.0	41.0
37	34.98837850527124	38.0	34.0	40.0	26.0	41.0
38	34.81623799564642	37.0	33.0	40.0	25.0	41.0
39	34.65820991079431	37.0	33.0	40.0	25.0	41.0
40	34.51318622220325	37.0	33.0	40.0	24.0	41.0
41	34.67758760510478	37.0	33.0	40.0	25.0	41.0
42	34.61079687566691	37.0	33.0	40.0	25.0	41.0
43	34.55808101071322	37.0	33.0	40.0	24.0	41.0
44	34.588107046822316	37.0	33.0	40.0	24.0	41.0
45	34.3634965214051	37.0	33.0	40.0	24.0	41.0
46	34.190729437876136	37.0	33.0	40.0	23.0	41.0
47	34.21844380895472	37.0	33.0	40.0	23.0	41.0
48	34.27790345298562	37.0	33.0	40.0	24.0	41.0
49	34.15496179947928	37.0	33.0	40.0	23.0	41.0
50	33.999892440991935	37.0	33.0	40.0	23.0	41.0
51	32.735905074907166	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	12.0
11	9.0
12	11.0
13	23.0
14	25.0
15	54.0
16	95.0
17	214.0
18	486.0
19	1018.0
20	1775.0
21	2751.0
22	3786.0
23	4482.0
24	5122.0
25	5785.0
26	6582.0
27	7878.0
28	9543.0
29	12311.0
30	16146.0
31	21054.0
32	27866.0
33	37465.0
34	53974.0
35	62662.0
36	72963.0
37	92096.0
38	95045.0
39	44485.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.89252635622519	35.89397754919118	19.144820521575827	27.068675573007813
2	19.878611976610184	36.9052029536045	19.155234965214053	24.06095010457126
3	18.782534465832942	32.95232404285288	23.258355030090915	25.006786461223268
4	20.457040419992317	31.624567843271162	19.234965214050963	28.68342652268556
5	15.237355414230228	37.443510179691835	19.58154424004439	27.73759016603355
6	21.224806863289086	34.48188142899825	22.473003542618123	21.82030816509454
7	72.6055742882752	10.359810491271501	9.926330615903368	7.108284604549916
8	73.9311110162619	7.034017670408469	7.861880575355329	11.172990737974306
9	67.98548807034017	10.8450211276623	14.565026249519825	6.604464552477698
10	29.286610610781512	37.81945452217337	14.906824875154722	17.987109991890392
11	21.365487216697254	27.89158734901191	32.47035724956251	18.27256818472833
12	23.300524990396518	21.504631012847327	37.31375645567459	17.881087541081566
13	24.5772333432925	28.812155875197405	27.20184386870972	19.408766912800377
14	13.566776217508217	33.64616500917666	34.172350505783434	18.61470826753169
15	12.281872892569039	29.600068291433697	36.28084852106364	21.83721029493363
16	13.143540057194075	31.32135387767297	31.819027700712795	23.716078364420163
17	13.64445772333433	27.662298860386702	29.992744035170087	28.700499381108884
18	19.43557130052499	25.79794272055999	28.02082888727645	26.74565709163857
19	19.847197917111274	32.158606854752655	28.846813777796747	19.147381450339324
20	18.991335524350163	29.923086772802936	26.839557812966834	24.246019889880063
21	18.02142643732127	32.06470613342439	26.410175423620302	23.503692005634043
22	14.170813948525332	32.58969652994153	26.902556660548893	26.336932860984252
23	17.353024030048232	29.67689615433864	23.88544111997951	29.08463869563362
24	13.012079047334499	32.246532075632764	26.36783473473046	28.373554142302275
25	15.711297964061632	29.357292244654058	28.342140082803365	26.589269708480945
26	18.200691450766143	30.351615519228307	24.823423961756795	26.62426906824875
27	14.323274574245593	28.532502454223398	28.031584788083148	29.112638183447864
28	19.599982927141575	25.89150198471979	32.674207179136964	21.834307909001666
29	17.30368346920483	29.602970677365658	27.51052114900337	25.582824704426137
30	19.711810149814333	26.154082547270473	30.04584062486662	24.088266678048573
31	18.863459814759487	29.298732340262067	25.543727858636732	26.29407998634171
32	18.927483033846944	28.968201801186567	24.684109437022496	27.420205727944
33	17.192197703700543	23.95441546800973	30.625122711169915	28.22826411711981
34	15.127235477399804	25.559605616970423	28.532160997054934	30.780997908574843
35	14.561611677835163	24.821716675914466	30.133424388578256	30.483247257672115
36	16.457040419992317	22.761534849972257	34.55341670579197	26.22800802424346
37	15.870075547398525	26.61095223867856	27.828417772845622	29.6905544410773
38	17.66306713901575	26.270860898885996	29.714968628622646	26.351103333475606
39	18.18771607836442	23.60271458448931	27.805027956805667	30.404541380340603
40	20.36382261300098	24.099022578855266	23.963634811558325	31.573519996585432
41	16.229117760040975	19.62712877203466	30.772290750778946	33.37146271714542
42	23.159503179819882	22.133424388578256	26.693926330615902	28.01314610098596
43	17.03649323487985	22.439540740108413	30.15749711895514	30.3664689060566
44	19.29386657561142	21.539630372615136	29.417047249135685	29.74945580263776
45	22.28161679969269	23.44615647274745	29.178027231209185	25.094199496350676
46	16.840155363011654	25.381535703615178	32.726620854496566	25.051688078876605
47	18.52063681761919	22.848094242178497	26.904946860728156	31.726322079474155
48	18.967945708310214	20.073584019804517	34.28059242818729	26.677877843697985
49	21.0593708651671	18.235520081949723	31.10794314738145	29.597165905501726
50	17.885867941440097	20.18216739937684	29.337487728883012	32.594476930300054
51	18.872508429723847	19.245208929104958	34.161594604976735	27.720688036194456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6090.0
1	4749.0
2	3408.0
3	2357.0
4	1306.0
5	1559.5
6	1813.0
7	1818.0
8	1823.0
9	1788.5
10	1754.0
11	1847.0
12	1940.0
13	2000.0
14	2060.0
15	1981.5
16	1903.0
17	1837.5
18	1772.0
19	1809.5
20	1847.0
21	1719.5
22	1592.0
23	1801.5
24	2011.0
25	2558.0
26	3352.5
27	3600.0
28	4149.0
29	4698.0
30	5705.5
31	6713.0
32	7461.5
33	8210.0
34	9625.5
35	11041.0
36	11909.0
37	12777.0
38	14494.5
39	16212.0
40	18051.0
41	19890.0
42	26649.0
43	33408.0
44	48834.0
45	64260.0
46	68571.0
47	72882.0
48	77112.0
49	81342.0
50	73956.0
51	66570.0
52	57453.5
53	48337.0
54	39644.5
55	30952.0
56	25855.0
57	20758.0
58	17867.0
59	14976.0
60	13507.5
61	12039.0
62	10226.0
63	8413.0
64	6766.5
65	5120.0
66	4302.0
67	3484.0
68	2893.5
69	2303.0
70	1906.0
71	1509.0
72	1508.5
73	1508.0
74	1255.0
75	803.5
76	605.0
77	439.5
78	274.0
79	228.5
80	183.0
81	159.0
82	135.0
83	102.0
84	69.0
85	43.0
86	17.0
87	12.0
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	585725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.766085339726374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49436223287546	31.214135072399202
2	9.592616408210084	7.629216054402835
3	3.520863883226083	4.2003292094978635
4	1.7695146321412953	2.8146667948650106
5	1.0727729513289308	2.132999036634819
6	0.7513190788307297	1.792621116368844
7	0.5682332019900453	1.5817487002241484
8	0.42308430703400224	1.3459525327530504
9	0.33074517513215734	1.1837196774007301
>10	3.148016740875052	26.2750885822174
>50	0.26882789942058727	6.817687623455295
>100	0.04667751307998816	3.6822869145308976
>500	0.007347386318146284	1.9448662181951755
>1k	0.0034575935614806043	2.2476988689958026
>5k	0.0021609959759253776	5.136983598058996
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	7981	1.362584830765291	TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6059	1.0344444918690512	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	5524	0.943104699304281	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5254	0.897007981561313	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5071	0.8657646506466344	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2995	0.5113321097784796	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2170	0.37048102778607706	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1458	0.24892227581202786	TruSeq Adapter, Index 19 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1406	0.24004438943190065	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	1318	0.22502027401937771	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1288	0.21989841649238123	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1257	0.21460583038115155	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1186	0.2024841009005933	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	961	0.16407016944811986	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	933	0.15928976908958983	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	915	0.15621665457339196	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	718	0.12258312347944854	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTTACCCTC	703	0.12002219471595033	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	662	0.1130223227623885	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	648	0.11063212258312348	TruSeq Adapter, Index 19 (95% over 22bp)
TACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	623	0.1063639079772931	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	609	0.10397370779802809	No Hit
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	609	0.10397370779802809	TruSeq Adapter, Index 19 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.10380297921379487	No Hit
CACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	601	0.10260787912416236	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	599	0.10226642195569594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7072858423321526E-4	0.0	0.0	1.6171411498570147	0.0
2	1.7072858423321526E-4	0.0	0.0	2.2298860386700246	0.0
3	1.7072858423321526E-4	0.0	0.0	3.1098211618080156	0.0
4	1.7072858423321526E-4	0.0	0.0	7.1682103376157755	0.0
5	1.7072858423321526E-4	0.0	0.0	7.998804899910367	0.0
6	1.7072858423321526E-4	0.0	0.0	11.101284732596355	0.0
7	1.7072858423321526E-4	0.0	0.0	13.601263391523325	0.0
8	3.414571684664305E-4	0.0	0.0	15.70737120662427	0.0
9	3.414571684664305E-4	0.0	0.0	18.695121430705537	0.0
10	3.414571684664305E-4	0.0	0.0	22.19198429297025	0.0
11	3.414571684664305E-4	0.0	0.0	25.864014682658244	0.0
12	3.414571684664305E-4	0.0	0.0	27.604251141747408	0.0
13	3.414571684664305E-4	0.0	0.0	28.55111186990482	0.0
14	3.414571684664305E-4	0.0	0.0	29.397925647701566	0.0
15	3.414571684664305E-4	0.0	0.0	29.973451705151735	0.0
16	3.414571684664305E-4	0.0	0.0	30.984335652396602	0.0
17	3.414571684664305E-4	0.0	0.0	32.231337231636005	0.0
18	3.414571684664305E-4	0.0	0.0	34.37244440650476	0.0
19	3.414571684664305E-4	0.0	0.0	35.025822698365275	0.0
20	3.414571684664305E-4	0.0	0.0	35.94280592428187	0.0
21	3.414571684664305E-4	0.0	0.0	36.61496436040804	0.0
22	3.414571684664305E-4	0.0	0.0	37.31375645567459	0.0
23	3.414571684664305E-4	0.0	0.0	37.95808613257075	0.0
24	3.414571684664305E-4	0.0	0.0	38.40778522344103	0.0
25	3.414571684664305E-4	0.0	0.0	38.8033633531094	0.0
26	3.414571684664305E-4	0.0	0.0	39.16633232318921	0.0
27	3.414571684664305E-4	0.0	0.0	39.68893251952708	0.0
28	3.414571684664305E-4	0.0	0.0	40.127704981006445	0.0
29	3.414571684664305E-4	0.0	0.0	40.55981902770071	0.0
30	3.414571684664305E-4	0.0	0.0	41.30061035468864	0.0
31	3.414571684664305E-4	0.0	0.0	41.70284689914209	0.0
32	3.414571684664305E-4	0.0	0.0	42.11891245891844	0.0
33	3.414571684664305E-4	0.0	0.0	42.5587092919032	0.0
34	3.414571684664305E-4	0.0	0.0	43.01523752614281	0.0
35	3.414571684664305E-4	0.0	0.0	43.45127833027445	0.0
36	3.414571684664305E-4	0.0	0.0	43.809296171411496	0.0
37	3.414571684664305E-4	0.0	0.0	44.17482607025481	0.0
38	3.414571684664305E-4	0.0	0.0	44.528575696786035	0.0
39	3.414571684664305E-4	0.0	0.0	44.97298220154509	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8048394E-9	45.000004	1
CGGAACG	40	6.8048394E-9	45.000004	1
GAGTGAC	20	7.0304493E-4	45.000004	9
AATTTGC	25	3.8884536E-5	45.000004	11
ATCTACG	25	3.8884536E-5	45.000004	1
CTCCGCG	20	7.0304493E-4	45.000004	1
CGAAACG	25	3.8884536E-5	45.000004	1
ACGCATG	20	7.0304493E-4	45.000004	1
GACGTCT	20	7.0304493E-4	45.000004	18
CGACCGG	20	7.0304493E-4	45.000004	2
TTTCCGG	50	2.1827873E-11	45.000004	2
ACGGGCC	40	6.8048394E-9	45.000004	5
TATTGCG	20	7.0304493E-4	45.000004	1
GCTCTCG	20	7.0304493E-4	45.000004	1
GTTGTAC	25	3.8884536E-5	45.000004	36
CGCTAAG	65	0.0	45.000004	1
TGACACG	25	3.8884536E-5	45.000004	1
GTCACGA	20	7.0304493E-4	45.000004	11
ACCCGTT	20	7.0304493E-4	45.000004	14
GCAATCG	20	7.0304493E-4	45.000004	1
>>END_MODULE