FastQCFastQC Report
Sat 18 Jun 2016
SRR3548716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476370
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC140052.939941642000966No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT123782.5984004030480508No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT121382.5480193966874487No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT65501.3749816319247643TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41490.8709616474589079No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT32630.6849717656443521No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT27510.577492285408401No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG26820.5630077460797279TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT22350.46917312173310666No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC17900.37575833910615697No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT14550.3054348510611499No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC12110.2542141612612045No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT10070.21139030585469276No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.20698196779814013No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC8600.180531939458824TruSeq Adapter, Index 19 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.16814660872850937No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.12889140793920692No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTGACACTGTCGTATGCCGT6070.127421961920356No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG5970.1253227533219976No Hit
AATCTGTCTCTTATACCCATCTGACGCTGACACTGTCGTATGCCGTCTTCT5830.1223838612842958No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA5530.11608623548922056No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.11482671033020551No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC5460.11461678947036968No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT5420.1137771060310263No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.10265130045972667No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.0291234E-445.013
AGACTCG502.1827873E-1145.01
GTACCGG207.0291234E-445.02
CGAACTC207.0291234E-445.030
TTGTGCG207.0291234E-445.01
TTTACCG207.0291234E-445.01
CCTAGCG207.0291234E-445.01
GCGAAGC207.0291234E-445.013
CCGGACG351.209919E-745.01
TAAGTAG406.8012014E-945.01
ATGTCGC207.0291234E-445.01
CCTAACG253.887355E-545.01
TTATCCG253.887355E-545.01
TAGCCGT253.887355E-545.044
TCGGCTC207.0291234E-445.031
CGTTATT253.887355E-545.016
AGTATAC207.0291234E-445.035
TAGCACG207.0291234E-445.01
AATGCAC207.0291234E-445.029
CTAACGG551.8189894E-1245.02
Produced by FastQC (version 0.11.5)