##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548716_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 476370 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.906864412116633 31.0 31.0 33.0 28.0 34.0 2 31.205823204651846 31.0 31.0 34.0 28.0 34.0 3 31.356743707622226 31.0 31.0 34.0 30.0 34.0 4 35.31899993702374 35.0 35.0 37.0 33.0 37.0 5 34.56552469718916 35.0 35.0 37.0 32.0 37.0 6 34.72143291978924 35.0 35.0 37.0 32.0 37.0 7 34.56893801037009 35.0 35.0 37.0 31.0 37.0 8 35.04119906795138 35.0 35.0 37.0 32.0 37.0 9 36.497844112769485 38.0 35.0 39.0 32.0 39.0 10 35.886147322459436 37.0 35.0 39.0 31.0 39.0 11 36.214562210046815 37.0 35.0 39.0 32.0 39.0 12 35.917958729558954 37.0 35.0 39.0 31.0 39.0 13 35.64979112874446 37.0 35.0 39.0 30.0 39.0 14 36.57533849738648 38.0 35.0 40.0 31.0 41.0 15 36.703866742238176 38.0 35.0 40.0 31.0 41.0 16 36.46527279215736 38.0 35.0 40.0 31.0 41.0 17 36.40645086802275 38.0 35.0 40.0 31.0 41.0 18 36.24714612591053 38.0 35.0 40.0 30.0 41.0 19 36.17965866868191 38.0 34.0 40.0 30.0 41.0 20 36.27869933035246 38.0 34.0 40.0 30.0 41.0 21 36.5060100342171 38.0 35.0 40.0 31.0 41.0 22 36.37971954573126 38.0 35.0 40.0 30.0 41.0 23 36.357320989986775 38.0 35.0 40.0 30.0 41.0 24 35.884927682263786 38.0 34.0 40.0 29.0 41.0 25 36.14185402103407 38.0 34.0 40.0 30.0 41.0 26 36.070226924449486 38.0 35.0 40.0 30.0 41.0 27 35.96055587043684 38.0 34.0 40.0 30.0 41.0 28 36.029149610596804 38.0 35.0 40.0 30.0 41.0 29 35.51653756533787 38.0 34.0 40.0 27.0 41.0 30 35.595469907844745 38.0 34.0 40.0 28.0 41.0 31 35.546709490522076 38.0 34.0 40.0 28.0 41.0 32 35.337231563700485 38.0 34.0 40.0 27.0 41.0 33 35.49325944119067 38.0 34.0 40.0 28.0 41.0 34 35.54612800974033 38.0 34.0 40.0 28.0 41.0 35 35.428037030039675 38.0 34.0 40.0 28.0 41.0 36 35.52554736864202 38.0 34.0 40.0 29.0 41.0 37 35.24864496084976 38.0 34.0 40.0 27.0 41.0 38 35.273986607049146 38.0 34.0 40.0 27.0 41.0 39 35.08020866133468 38.0 34.0 40.0 27.0 41.0 40 34.89944161051284 38.0 34.0 40.0 26.0 41.0 41 35.1368327140668 38.0 34.0 40.0 27.0 41.0 42 35.10437055230178 38.0 34.0 40.0 27.0 41.0 43 35.14248168440498 38.0 34.0 40.0 27.0 41.0 44 35.0950122803703 38.0 34.0 40.0 27.0 41.0 45 34.875073997103094 37.0 34.0 40.0 26.0 41.0 46 34.81003211789155 37.0 33.0 40.0 26.0 41.0 47 34.76242206688079 37.0 33.0 40.0 26.0 41.0 48 34.879696454436676 37.0 34.0 40.0 26.0 41.0 49 34.75177907928711 37.0 34.0 40.0 26.0 41.0 50 34.69182148330079 37.0 34.0 40.0 26.0 41.0 51 33.34415895207507 36.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 5.0 11 7.0 12 12.0 13 17.0 14 17.0 15 37.0 16 90.0 17 167.0 18 343.0 19 727.0 20 1249.0 21 1860.0 22 2534.0 23 3097.0 24 3471.0 25 3941.0 26 4447.0 27 5457.0 28 6927.0 29 9074.0 30 12058.0 31 16701.0 32 23159.0 33 31290.0 34 46193.0 35 51810.0 36 57582.0 37 73007.0 38 79388.0 39 41697.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.344711043936435 42.930285282448516 16.96601381279258 22.75898986082247 2 18.745093099901336 41.83092973948821 18.115750362113484 21.308226798496964 3 17.73873249784831 29.382412830362952 31.022524508260386 21.85633016352835 4 27.077901631085084 28.991540189348612 17.590738291664042 26.33981988790226 5 14.869324264752187 40.76956987215819 19.683229422507715 24.6778764405819 6 19.83038394525264 31.590360434116338 28.593740159959697 19.985515460671326 7 66.78296282301572 15.91577975103386 10.250645506643995 7.050611919306421 8 68.70457837395301 6.08896446039843 7.4578583873879545 17.748598778260597 9 68.04290782375044 9.345046917312173 14.297080000839685 8.314965258097697 10 32.30514096185738 33.06001637382707 16.58563721477003 18.04920544954552 11 23.800827088187752 23.91565379851796 31.028402292335787 21.2551168209585 12 25.207296849087896 21.155404412536473 37.59850536347797 16.038793374897665 13 23.565925646031445 31.981862837710185 24.762054705376073 19.690156810882296 14 13.940424459978587 31.147847261582385 36.78989021139031 18.121838067048724 15 12.24909209228121 29.89461972836241 31.80468963200873 26.05159854734765 16 13.798937800449233 27.906669185716986 35.01710855007662 23.277284463757162 17 12.630518294602936 26.751264773180512 30.9763419190965 29.641875013120057 18 16.115834330457417 26.6305602787749 28.961731427251923 28.291873963515755 19 17.57310493943783 31.415076516153412 32.523248735226815 18.488569809181936 20 19.084954971975566 32.67901001322501 27.006948380460567 21.229086634338852 21 15.423935176438484 30.02351113630161 28.958582614354388 25.593971072905514 22 15.00157440644877 33.796208829271364 27.52272393307723 23.679492831202637 23 19.249952767806537 28.525096038793375 22.326133047840965 29.898818145559126 24 12.92209836891492 36.37466675063501 26.51741293532338 24.185821945126687 25 15.483552700631861 26.742448097067406 29.727732644792916 28.04626655750782 26 20.929739488212945 31.642630728215465 23.367130591766905 24.06049919180469 27 13.85855532464261 30.371140080189768 29.666855595440516 26.103448999727103 28 19.325314356487606 25.31708545878204 31.964229485483976 23.393370699246386 29 21.40793920691899 31.21670130360854 26.8730188718853 20.50234061758717 30 19.28626907655814 30.428238554065118 24.52547389634108 25.760018473035668 31 23.099061653756532 30.20509268005962 21.643050569935134 25.052795096248715 32 21.639481915317926 30.359594432898795 20.873060856057265 27.12786279572601 33 20.68413208220501 26.43134538279069 26.046350525851754 26.83817200915255 34 19.213636459054936 25.93845120389613 28.603186598652307 26.244725738396625 35 22.199340848500114 27.20574343472511 27.177194197787436 23.417721518987342 36 22.223061905661563 24.092617083359574 31.029032054915294 22.65528895606356 37 19.405923966664567 32.62820916514474 23.177572055335137 24.788294812855554 38 21.075214644079182 26.358922686147324 25.90045552826584 26.665407141507654 39 18.219871108592063 26.963074920754877 30.940865293784242 23.87618867686882 40 23.095702919999162 26.09211327329597 24.314713353065894 26.49747045363898 41 19.118542309549298 20.385834540378276 36.60704914247329 23.888574007599132 42 27.583600982429623 23.44899972710288 25.489640405567098 23.477758884900393 43 21.512269874257406 22.804542687406848 29.918550706383694 25.764636731952056 44 19.949828914499236 23.251464197997354 27.33757373470202 29.461133152801395 45 25.92837500262401 26.77729495980016 25.368935911161493 21.92539412641434 46 17.60564267271239 23.979679660767893 36.65092260217897 21.763755064340742 47 21.20305644771921 25.38803870940655 24.94993387492915 28.458970967945085 48 19.532926086865253 20.038205596490126 37.906669185716986 22.52219913092764 49 23.698385708587864 19.72878224909209 30.69105947057959 25.881772571740452 50 19.189705481033652 21.037428889308732 27.55987992526817 32.212985704389446 51 17.300417742511073 20.60835065180427 36.28293133488675 25.808300270797908 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4790.0 1 3779.0 2 2768.0 3 1848.5 4 929.0 5 1169.5 6 1410.0 7 1315.0 8 1220.0 9 1204.0 10 1188.0 11 1180.5 12 1173.0 13 1198.5 14 1224.0 15 1244.0 16 1264.0 17 1254.5 18 1245.0 19 1310.0 20 1375.0 21 1407.5 22 1440.0 23 1485.5 24 1531.0 25 2334.5 26 3235.5 27 3333.0 28 3842.5 29 4352.0 30 5019.0 31 5686.0 32 6926.0 33 8166.0 34 8933.5 35 9701.0 36 10765.5 37 11830.0 38 12832.5 39 13835.0 40 15471.5 41 17108.0 42 22223.5 43 27339.0 44 48797.5 45 70256.0 46 69602.5 47 68949.0 48 64223.5 49 59498.0 50 52834.0 51 46170.0 52 39550.5 53 32931.0 54 27632.5 55 22334.0 56 19093.5 57 15853.0 58 13185.5 59 10518.0 60 9192.0 61 7866.0 62 6923.5 63 5981.0 64 4761.0 65 3541.0 66 2839.5 67 2138.0 68 1706.5 69 1275.0 70 1176.5 71 1078.0 72 1010.0 73 942.0 74 697.5 75 347.5 76 242.0 77 183.0 78 124.0 79 115.0 80 106.0 81 77.5 82 49.0 83 28.5 84 8.0 85 7.5 86 7.0 87 4.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 1.5 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 476370.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.43548169720168 #Duplication Level Percentage of deduplicated Percentage of total 1 78.57708113580763 27.05839639271635 2 9.263516586199714 6.379873117116089 3 3.3118762419494336 3.4213816113914044 4 1.6753209869652304 2.3076194073431617 5 1.0148412854440654 1.747327425523687 6 0.7061677443315605 1.4590335861050188 7 0.5220725231946501 1.2584473171956891 8 0.44710941356553663 1.2317142421986091 9 0.35888303710599834 1.1122479230129783 >10 3.7486567726554827 28.186701773923104 >50 0.3015406582784185 6.585206084386734 >100 0.058224314098475116 3.8770026206712496 >500 0.006741762685086593 1.52716336217622 >1k 0.0055159876514344845 4.335615941015213 >5k 6.128875168260539E-4 1.3823825348610062 >10k+ 0.0018386625504781613 8.129886660363484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC 14005 2.939941642000966 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT 12378 2.5984004030480508 No Hit AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT 12138 2.5480193966874487 No Hit CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT 6550 1.3749816319247643 TruSeq Adapter, Index 13 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4149 0.8709616474589079 No Hit AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT 3263 0.6849717656443521 No Hit AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT 2751 0.577492285408401 No Hit AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG 2682 0.5630077460797279 TruSeq Adapter, Index 19 (95% over 21bp) AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT 2235 0.46917312173310666 No Hit AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC 1790 0.37575833910615697 No Hit AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT 1455 0.3054348510611499 No Hit AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC 1211 0.2542141612612045 No Hit AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT 1007 0.21139030585469276 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 986 0.20698196779814013 No Hit CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC 860 0.180531939458824 TruSeq Adapter, Index 19 (95% over 22bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 801 0.16814660872850937 No Hit AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT 614 0.12889140793920692 No Hit AATGATACCTGTCTCTTCTACACATCTGACGCTGACACTGTCGTATGCCGT 607 0.127421961920356 No Hit AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG 597 0.1253227533219976 No Hit AATCTGTCTCTTATACCCATCTGACGCTGACACTGTCGTATGCCGTCTTCT 583 0.1223838612842958 No Hit AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA 553 0.11608623548922056 No Hit GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.11482671033020551 No Hit AATGATACGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC 546 0.11461678947036968 No Hit AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT 542 0.1137771060310263 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 489 0.10265130045972667 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.099208598358419E-4 0.0 0.0 1.626256901148267 0.0 2 2.099208598358419E-4 0.0 0.0 2.1264983101370785 0.0 3 2.099208598358419E-4 0.0 0.0 3.238239183827697 0.0 4 2.099208598358419E-4 0.0 0.0 8.318533912714907 0.0 5 2.099208598358419E-4 0.0 0.0 9.041291433129711 0.0 6 2.099208598358419E-4 0.0 0.0 11.702248252408841 0.0 7 2.099208598358419E-4 0.0 0.0 13.86107437496064 0.0 8 2.099208598358419E-4 0.0 0.0 15.27866994143208 0.0 9 2.099208598358419E-4 0.0 0.0 19.59275353191847 0.0 10 2.099208598358419E-4 0.0 0.0 22.088502634506792 0.0 11 2.099208598358419E-4 0.0 0.0 27.43749606398388 0.0 12 2.099208598358419E-4 0.0 0.0 28.79988244431849 0.0 13 2.099208598358419E-4 0.0 0.0 29.568192791317674 0.0 14 2.099208598358419E-4 0.0 0.0 30.591137141297732 0.0 15 2.099208598358419E-4 0.0 0.0 31.119927787224217 0.0 16 2.099208598358419E-4 0.0 0.0 31.9497449461553 0.0 17 2.099208598358419E-4 0.0 0.0 32.91097256334362 0.0 18 2.099208598358419E-4 0.0 0.0 34.87667149484644 0.0 19 2.099208598358419E-4 0.0 0.0 35.48187333375318 0.0 20 2.099208598358419E-4 0.0 0.0 36.087285093519746 0.0 21 2.099208598358419E-4 0.0 0.0 36.707181392614984 0.0 22 2.099208598358419E-4 0.0 0.0 37.28341415286437 0.0 23 2.099208598358419E-4 0.0 0.0 37.82081155404413 0.0 24 2.099208598358419E-4 0.0 0.0 38.214833007956 0.0 25 2.099208598358419E-4 0.0 0.0 38.59101118878183 0.0 26 2.099208598358419E-4 0.0 0.0 38.90820160799379 0.0 27 2.099208598358419E-4 0.0 0.0 39.322585385309736 0.0 28 2.099208598358419E-4 0.0 0.0 39.673153221235594 0.0 29 2.099208598358419E-4 0.0 0.0 40.03652622961144 0.0 30 2.099208598358419E-4 0.0 0.0 40.66691857169847 0.0 31 2.099208598358419E-4 0.0 0.0 41.021894745680875 0.0 32 2.099208598358419E-4 0.0 0.0 41.35860780485757 0.0 33 2.099208598358419E-4 0.0 0.0 41.695530784894096 0.0 34 2.099208598358419E-4 0.0 0.0 41.98627117576674 0.0 35 2.099208598358419E-4 0.0 0.0 42.35237315532044 0.0 36 2.099208598358419E-4 0.0 0.0 42.66620484077503 0.0 37 2.099208598358419E-4 0.0 0.0 42.93931187942146 0.0 38 2.099208598358419E-4 0.0 0.0 43.262170161848985 0.0 39 2.099208598358419E-4 0.0 0.0 43.56109746625522 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACGA 20 7.0291234E-4 45.0 13 AGACTCG 50 2.1827873E-11 45.0 1 GTACCGG 20 7.0291234E-4 45.0 2 CGAACTC 20 7.0291234E-4 45.0 30 TTGTGCG 20 7.0291234E-4 45.0 1 TTTACCG 20 7.0291234E-4 45.0 1 CCTAGCG 20 7.0291234E-4 45.0 1 GCGAAGC 20 7.0291234E-4 45.0 13 CCGGACG 35 1.209919E-7 45.0 1 TAAGTAG 40 6.8012014E-9 45.0 1 ATGTCGC 20 7.0291234E-4 45.0 1 CCTAACG 25 3.887355E-5 45.0 1 TTATCCG 25 3.887355E-5 45.0 1 TAGCCGT 25 3.887355E-5 45.0 44 TCGGCTC 20 7.0291234E-4 45.0 31 CGTTATT 25 3.887355E-5 45.0 16 AGTATAC 20 7.0291234E-4 45.0 35 TAGCACG 20 7.0291234E-4 45.0 1 AATGCAC 20 7.0291234E-4 45.0 29 CTAACGG 55 1.8189894E-12 45.0 2 >>END_MODULE