FastQCFastQC Report
Sat 18 Jun 2016
SRR3548715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486656
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC148793.057395778537612No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT130532.6821820752235666No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT130122.6737572330352446No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT68001.397290899526565TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41720.85727906365071No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT33060.6793299579168859No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT29590.6080270252498685No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG27120.5572724881641241TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT22350.4592566412414518No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC18100.37192596002104156No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT15800.3246646501841136No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC12020.24699171488690164No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT10570.21719654129405577No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10510.21596363755917938No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8490.17445587848500788No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC8440.17342845870594423TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG6460.13274263545502366No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTGACACTGTCGTATGCCGT6320.12986586007364545No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.12349585744345082No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT5840.1200026301946344No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC5400.11096133613887427No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.10993391635981062No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA5140.1056187532877433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA253.8874787E-545.0000046
GACAGCG253.8874787E-545.0000041
CGAGTCA253.8874787E-545.00000431
GTTTAGC253.8874787E-545.00000418
TTCCGAA253.8874787E-545.00000429
CGCGAGT253.8874787E-545.00000429
AGTCTAA253.8874787E-545.00000416
AGCGAAG502.1827873E-1145.0000041
CGGCTTG253.8874787E-545.0000041
AAGGGTC253.8874787E-545.0000045
ATGCGAG253.8874787E-545.0000041
CGAGACA650.045.00000422
ACGTAAG253.8874787E-545.0000041
GCGGAAC253.8874787E-545.0000045
GCGAGTC253.8874787E-545.00000430
CTAGCGG502.1827873E-1145.0000042
GCATAGA253.8874787E-545.00000423
CGGAACG207.029273E-445.01
TTTCGCG351.2099918E-745.01
GACCGAA207.029273E-445.09