##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548715_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486656
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.981911247369805	31.0	31.0	33.0	28.0	34.0
2	31.272574467385585	31.0	31.0	34.0	30.0	34.0
3	31.435104057075222	31.0	31.0	34.0	30.0	34.0
4	35.36737449039979	37.0	35.0	37.0	33.0	37.0
5	34.581338769068914	35.0	35.0	37.0	32.0	37.0
6	34.73098451472909	35.0	35.0	37.0	32.0	37.0
7	34.593723287085744	35.0	35.0	37.0	31.0	37.0
8	35.03396033337717	35.0	35.0	37.0	32.0	37.0
9	36.584252531562335	38.0	35.0	39.0	32.0	39.0
10	35.94964204694897	37.0	35.0	39.0	31.0	39.0
11	36.21528965018411	37.0	35.0	39.0	32.0	39.0
12	35.89980807798527	37.0	35.0	39.0	31.0	39.0
13	35.713378649395054	37.0	35.0	39.0	30.0	39.0
14	36.622394463440294	38.0	35.0	40.0	31.0	41.0
15	36.7275097810363	38.0	35.0	40.0	31.0	41.0
16	36.50697001578117	38.0	35.0	40.0	31.0	41.0
17	36.48052628550763	38.0	35.0	40.0	31.0	41.0
18	36.24222037743293	38.0	35.0	40.0	30.0	41.0
19	36.257991271041554	38.0	34.0	40.0	30.0	41.0
20	36.29028512953708	38.0	34.0	40.0	30.0	41.0
21	36.50535491188848	38.0	35.0	40.0	31.0	41.0
22	36.421005802866915	38.0	35.0	40.0	30.0	41.0
23	36.355902321146765	38.0	35.0	40.0	30.0	41.0
24	35.89470385652288	38.0	34.0	40.0	29.0	41.0
25	36.11206067530247	38.0	34.0	40.0	30.0	41.0
26	36.00141372961599	38.0	34.0	40.0	30.0	41.0
27	35.84988575092057	38.0	34.0	40.0	29.0	41.0
28	35.886209149789586	38.0	34.0	40.0	29.0	41.0
29	35.49450741386113	38.0	34.0	40.0	27.0	41.0
30	35.57616057338243	38.0	34.0	40.0	28.0	41.0
31	35.556491649132035	38.0	34.0	40.0	29.0	41.0
32	35.330964788269334	38.0	34.0	40.0	27.0	41.0
33	35.47691387756444	38.0	34.0	40.0	28.0	41.0
34	35.52588481391373	38.0	34.0	40.0	28.0	41.0
35	35.36959166228301	38.0	34.0	40.0	27.0	41.0
36	35.455379158995264	38.0	34.0	40.0	28.0	41.0
37	35.16214533469227	38.0	34.0	40.0	27.0	41.0
38	35.24431631378222	38.0	34.0	40.0	27.0	41.0
39	35.07827294844819	38.0	34.0	40.0	27.0	41.0
40	34.86343125328774	37.0	33.0	40.0	26.0	41.0
41	35.096772668990006	38.0	34.0	40.0	27.0	41.0
42	35.08115999802735	38.0	34.0	40.0	27.0	41.0
43	35.14058184836928	38.0	34.0	40.0	27.0	41.0
44	35.037870693056284	38.0	34.0	40.0	26.0	41.0
45	34.80034562401368	37.0	33.0	40.0	26.0	41.0
46	34.71512937269858	37.0	33.0	40.0	25.0	41.0
47	34.649460810099946	37.0	33.0	40.0	26.0	41.0
48	34.76792025578643	37.0	33.0	40.0	26.0	41.0
49	34.60776811546555	37.0	33.0	40.0	26.0	41.0
50	34.54078651038927	37.0	33.0	40.0	25.0	41.0
51	33.121775956733295	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	4.0
12	12.0
13	11.0
14	19.0
15	42.0
16	74.0
17	174.0
18	350.0
19	662.0
20	1248.0
21	1872.0
22	2581.0
23	3170.0
24	3509.0
25	3910.0
26	4513.0
27	5568.0
28	7298.0
29	9380.0
30	12781.0
31	17578.0
32	23780.0
33	32317.0
34	46974.0
35	53485.0
36	59175.0
37	74096.0
38	80021.0
39	42047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.163869345081537	43.3174562730142	16.80447790636507	22.71419647553919
2	18.607394134665967	42.35969555497107	17.99957259337191	21.033337716991056
3	17.631550828511312	29.278586927932665	31.39897586796423	21.690886375591795
4	27.343955483955813	28.764260586533403	17.591481457127827	26.30030247238296
5	14.81292740662809	41.02836500526038	19.61323809836928	24.545469489742242
6	19.91612144923724	31.443360402419778	28.725013150973172	19.91550499736981
7	66.35076933193056	16.080763413992635	10.371391701735929	7.197075552340873
8	68.32279885586533	6.046159915833772	7.597769266175697	18.0332719621252
9	67.78648573119412	9.24924381904261	14.502646633350869	8.461623816412414
10	32.206733298264076	32.88400841662283	16.763997567069964	18.145260718043136
11	23.721478826933193	23.851961138874277	31.181779326670174	21.244780707522356
12	24.849996712256708	21.179025841662284	38.00466859547606	15.966308850604946
13	23.49113131246712	31.863780576012623	24.770679905312992	19.87440820620726
14	13.80893279852709	31.04081733298264	37.249926025775906	17.90032384271436
15	12.294310560231457	29.972300762756444	31.555965610205156	26.17742306680694
16	13.732287283008942	27.832596330878484	35.13344128090479	23.301675105207785
17	12.581166162546028	26.902575946870066	31.199245462914256	29.31701242766965
18	15.864183324566017	26.96730339295108	28.86844095213046	28.300072330352442
19	17.283255523408734	31.461032022619673	32.87784389794845	18.377868556023145
20	18.96164847448711	32.846815820620726	27.109498290373484	21.082037414518677
21	15.2666359810626	30.031891109942137	28.93029162283009	25.771181286165174
22	14.905806154655444	33.95190853498159	27.58026203314045	23.562023277222515
23	19.30624506838506	28.357402354024195	22.250008219358232	30.08634435823251
24	12.853432403997896	36.60162414518675	26.49037513150973	24.054568319305627
25	15.478695423461335	26.592706141504475	29.812023277222515	28.11657515781168
26	21.08018805891636	31.720558258811156	23.264071541294058	23.935182140978434
27	13.774206009994739	30.30025315623356	29.896888150973172	26.028652682798526
28	19.347547343503418	25.26712914255655	31.668776301946345	23.71654721199369
29	21.526704694897422	31.17171061283535	26.85531463703314	20.446270055234088
30	19.328437335612836	30.533477446081008	24.328067464492374	25.81001775381378
31	22.984818845344556	30.252169910573386	21.731366714886903	25.031644529195162
32	21.569239873750657	30.396830615465543	20.82518246975276	27.208747041031035
33	20.767030510257758	26.378386375591795	26.043036559705417	26.81154655444503
34	19.32494410836402	25.790496778011573	28.586722448711203	26.297836664913206
35	22.327064702788007	27.3647093634929	27.042921488690162	23.26530444502893
36	22.19103432403998	23.909907614413466	31.072461862177803	22.826596199368755
37	19.39049349026828	32.74160803524461	23.108314702788007	24.759583771699106
38	21.4069075486586	26.1492717648606	25.79666129668595	26.647159389794844
39	18.22765978432404	26.91695982377696	31.232328379800105	23.623052012098896
40	23.030847251446605	26.065434310889007	24.2171061283535	26.68661230931089
41	19.18500953445555	20.133934442398736	36.841629405576015	23.8394266175697
42	27.889720870594424	23.29900381378222	25.378090478695427	23.433184836927936
43	21.599240531299316	22.72693648079958	29.93593010257759	25.737892885323514
44	19.91632693319306	23.089204694897422	27.44443713834824	29.550031233561285
45	26.253452130457656	26.5914732377696	25.32014400315623	21.834930628616515
46	17.457094950026303	23.702985270910048	36.93471363755918	21.905206141504472
47	21.1260109810626	25.40048822987901	24.885956404523935	28.587544384534457
48	19.582415504997368	19.83516077064703	38.08090314308259	22.501520581273013
49	23.663737835349817	19.684951998947923	30.671357180431354	25.97995298527091
50	19.288984416096792	20.941075420831144	27.396148408732245	32.37379175433982
51	17.127704168858497	20.453667477643343	36.49867668332457	25.919951670173592
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4788.0
1	3834.0
2	2880.0
3	1922.5
4	965.0
5	1154.5
6	1344.0
7	1280.0
8	1216.0
9	1193.0
10	1170.0
11	1194.5
12	1219.0
13	1234.0
14	1249.0
15	1295.0
16	1341.0
17	1309.5
18	1278.0
19	1376.0
20	1474.0
21	1465.0
22	1456.0
23	1493.0
24	1530.0
25	2371.0
26	3309.5
27	3407.0
28	4001.5
29	4596.0
30	5273.5
31	5951.0
32	7130.0
33	8309.0
34	9109.5
35	9910.0
36	10884.0
37	11858.0
38	13044.5
39	14231.0
40	15840.0
41	17449.0
42	22693.5
43	27938.0
44	50533.5
45	73129.0
46	72007.0
47	70885.0
48	65900.5
49	60916.0
50	53643.5
51	46371.0
52	39912.5
53	33454.0
54	28055.0
55	22656.0
56	19244.0
57	15832.0
58	13197.5
59	10563.0
60	9281.5
61	8000.0
62	7015.0
63	6030.0
64	4831.0
65	3632.0
66	2865.0
67	2098.0
68	1714.5
69	1331.0
70	1234.5
71	1138.0
72	1002.5
73	867.0
74	659.0
75	339.5
76	228.0
77	165.5
78	103.0
79	108.0
80	113.0
81	83.0
82	53.0
83	31.0
84	9.0
85	8.5
86	8.0
87	7.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	4.0
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.16149413792283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6583024828736	26.870851391676474
2	9.193407418667645	6.2812106728070205
3	3.3179090170140104	3.4003418830465626
4	1.6360169608810122	2.235551352747161
5	1.0012489231731045	1.7102079609789778
6	0.7159092470122042	1.4673917727055297
7	0.5357520395670294	1.2811463111334493
8	0.43867156758375303	1.1988540947588637
9	0.37404692460207495	1.1500201639891663
>10	3.7309805994993046	27.81111235588839
>50	0.31989386060231234	6.867765361017715
>100	0.06397877212046248	4.143175128025998
>500	0.005432159897020399	1.184559625286621
>1k	0.0060357332189115535	4.553492561328066
>5k	6.035733218911554E-4	1.4020897218362094
>10k+	0.0018107199656734662	8.442229642773787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	14879	3.057395778537612	No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	13053	2.6821820752235666	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	13012	2.6737572330352446	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	6800	1.397290899526565	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4172	0.85727906365071	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	3306	0.6793299579168859	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	2959	0.6080270252498685	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	2712	0.5572724881641241	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	2235	0.4592566412414518	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	1810	0.37192596002104156	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	1580	0.3246646501841136	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	1202	0.24699171488690164	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	1057	0.21719654129405577	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1051	0.21596363755917938	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.17445587848500788	No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	844	0.17342845870594423	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG	646	0.13274263545502366	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTGACACTGTCGTATGCCGT	632	0.12986586007364545	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.12349585744345082	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	584	0.1200026301946344	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	540	0.11096133613887427	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.10993391635981062	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA	514	0.1056187532877433	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0548395581273014E-4	0.0	0.0	1.6901055365597055	0.0
2	2.0548395581273014E-4	0.0	0.0	2.1680612177801155	0.0
3	2.0548395581273014E-4	0.0	0.0	3.3082916885849554	0.0
4	2.0548395581273014E-4	0.0	0.0	8.52141964755392	0.0
5	2.0548395581273014E-4	0.0	0.0	9.216571870068385	0.0
6	2.0548395581273014E-4	0.0	0.0	11.890740071015255	0.0
7	2.0548395581273014E-4	0.0	0.0	14.134419384534455	0.0
8	2.0548395581273014E-4	0.0	0.0	15.551025775907418	0.0
9	2.0548395581273014E-4	0.0	0.0	19.987835349815885	0.0
10	2.0548395581273014E-4	0.0	0.0	22.458779918463968	0.0
11	2.0548395581273014E-4	0.0	0.0	27.92239281956865	0.0
12	2.0548395581273014E-4	0.0	0.0	29.306327261967386	0.0
13	2.0548395581273014E-4	0.0	0.0	30.06065886375592	0.0
14	2.0548395581273014E-4	0.0	0.0	31.073283798001054	0.0
15	2.0548395581273014E-4	0.0	0.0	31.586788203577065	0.0
16	2.0548395581273014E-4	0.0	0.0	32.41529951341399	0.0
17	2.0548395581273014E-4	0.0	0.0	33.35230635192004	0.0
18	2.0548395581273014E-4	0.0	0.0	35.29865038137822	0.0
19	2.0548395581273014E-4	0.0	0.0	35.9148967648606	0.0
20	2.0548395581273014E-4	0.0	0.0	36.51264959231983	0.0
21	2.0548395581273014E-4	0.0	0.0	37.0997172540768	0.0
22	2.0548395581273014E-4	0.0	0.0	37.674044910573386	0.0
23	2.0548395581273014E-4	0.0	0.0	38.22700223566544	0.0
24	2.0548395581273014E-4	0.0	0.0	38.626463045765384	0.0
25	2.0548395581273014E-4	0.0	0.0	38.98277222514466	0.0
26	2.0548395581273014E-4	0.0	0.0	39.286682995791686	0.0
27	2.0548395581273014E-4	0.0	0.0	39.674225736454495	0.0
28	2.0548395581273014E-4	0.0	0.0	40.01101394003156	0.0
29	2.0548395581273014E-4	0.0	0.0	40.359103761178325	0.0
30	2.0548395581273014E-4	0.0	0.0	40.97432272488164	0.0
31	2.0548395581273014E-4	0.0	0.0	41.33474158337717	0.0
32	2.0548395581273014E-4	0.0	0.0	41.6548855865334	0.0
33	2.0548395581273014E-4	0.0	0.0	41.98324894792215	0.0
34	2.0548395581273014E-4	0.0	0.0	42.27483068122041	0.0
35	2.0548395581273014E-4	0.0	0.0	42.639975670699634	0.0
36	2.0548395581273014E-4	0.0	0.0	42.95703741451867	0.0
37	2.0548395581273014E-4	0.0	0.0	43.244509468700684	0.0
38	2.0548395581273014E-4	0.0	0.0	43.554584758022095	0.0
39	2.0548395581273014E-4	0.0	0.0	43.85602972119937	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	25	3.8874787E-5	45.000004	6
GACAGCG	25	3.8874787E-5	45.000004	1
CGAGTCA	25	3.8874787E-5	45.000004	31
GTTTAGC	25	3.8874787E-5	45.000004	18
TTCCGAA	25	3.8874787E-5	45.000004	29
CGCGAGT	25	3.8874787E-5	45.000004	29
AGTCTAA	25	3.8874787E-5	45.000004	16
AGCGAAG	50	2.1827873E-11	45.000004	1
CGGCTTG	25	3.8874787E-5	45.000004	1
AAGGGTC	25	3.8874787E-5	45.000004	5
ATGCGAG	25	3.8874787E-5	45.000004	1
CGAGACA	65	0.0	45.000004	22
ACGTAAG	25	3.8874787E-5	45.000004	1
GCGGAAC	25	3.8874787E-5	45.000004	5
GCGAGTC	25	3.8874787E-5	45.000004	30
CTAGCGG	50	2.1827873E-11	45.000004	2
GCATAGA	25	3.8874787E-5	45.000004	23
CGGAACG	20	7.029273E-4	45.0	1
TTTCGCG	35	1.2099918E-7	45.0	1
GACCGAA	20	7.029273E-4	45.0	9
>>END_MODULE