FastQCFastQC Report
Sat 18 Jun 2016
SRR3548714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452519
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT44970.993770427319074No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT42570.9407339802306643No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC35040.7743321274907794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34090.7533385338516173No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT32220.7120143021618982No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT11130.2459565233724993No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTG10630.23490726356241395No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT9690.21413465511945354No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCT8850.1955718986385102No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8350.1845226388284249No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT7690.16993761587911227No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7550.16684382313228838No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC7470.16507594156267472No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.1577834300880184No Hit
AATGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTC5800.12817141379698974No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTAT5500.12154185791093855No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.10563092378441567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAG302.1625729E-645.0000041
CATGACG302.1625729E-645.0000041
CGTTCGA351.2097371E-745.00000414
CCAGACG302.1625729E-645.0000041
GTTCGAA351.2097371E-745.00000415
CGTCGCC351.2097371E-745.00000427
CGCACCG302.1625729E-645.0000041
CCGGGTG207.028749E-445.05
CGAACCG207.028749E-445.01
CGACATG207.028749E-445.01
TACGGGT406.7993824E-945.04
CGCTGCC207.028749E-445.025
CGTTCAT502.1827873E-1145.017
AATATGG253.8870443E-545.02
CTGCGCG253.8870443E-545.01
CTAACCG207.028749E-445.01
AACGCGC207.028749E-445.016
CGTACAT453.8380676E-1045.035
ACGTCAC207.028749E-445.025
GGCGCAG207.028749E-445.09