##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548714_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 452519 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.996355954114634 31.0 31.0 33.0 28.0 34.0 2 31.29757203564933 31.0 31.0 34.0 28.0 34.0 3 31.460228189313597 31.0 31.0 34.0 30.0 34.0 4 35.431188524680735 37.0 35.0 37.0 33.0 37.0 5 34.738525896150215 35.0 35.0 37.0 32.0 37.0 6 34.82602277473432 35.0 35.0 37.0 32.0 37.0 7 34.88428773156486 35.0 35.0 37.0 32.0 37.0 8 35.268704739469506 37.0 35.0 37.0 33.0 37.0 9 36.65954799687969 39.0 37.0 39.0 32.0 39.0 10 35.997014489999316 37.0 35.0 39.0 31.0 39.0 11 36.30234089618337 37.0 35.0 39.0 32.0 39.0 12 36.21246400703617 37.0 35.0 39.0 32.0 39.0 13 35.92530700368383 37.0 35.0 39.0 31.0 39.0 14 36.940413551696174 39.0 36.0 40.0 31.0 41.0 15 36.97334476563415 38.0 36.0 40.0 32.0 41.0 16 36.822608553453 38.0 35.0 40.0 31.0 41.0 17 36.7490867786767 38.0 35.0 40.0 31.0 41.0 18 36.67379049277489 38.0 35.0 40.0 31.0 41.0 19 36.70278154066459 38.0 35.0 40.0 31.0 41.0 20 36.61650450036352 38.0 35.0 40.0 31.0 41.0 21 36.76748158640853 38.0 35.0 40.0 31.0 41.0 22 36.74019212452958 39.0 35.0 40.0 31.0 41.0 23 36.67375955484742 38.0 35.0 40.0 31.0 41.0 24 36.20590737626486 38.0 35.0 40.0 30.0 41.0 25 36.397094928610734 38.0 35.0 40.0 30.0 41.0 26 36.33583783222362 38.0 35.0 40.0 30.0 41.0 27 36.269679284184754 38.0 35.0 40.0 30.0 41.0 28 36.14821477109248 38.0 35.0 40.0 30.0 41.0 29 35.5969340513879 38.0 34.0 40.0 27.0 41.0 30 35.70604549201249 38.0 34.0 40.0 28.0 41.0 31 35.5259602359238 38.0 34.0 40.0 27.0 41.0 32 35.556343490549565 38.0 34.0 40.0 28.0 41.0 33 35.48728119703261 38.0 34.0 40.0 27.0 41.0 34 35.32797738879472 38.0 34.0 40.0 27.0 41.0 35 35.37017893171336 38.0 34.0 40.0 27.0 41.0 36 35.322474857409304 38.0 34.0 40.0 27.0 41.0 37 35.29531356694415 38.0 34.0 40.0 27.0 41.0 38 35.24621065634813 38.0 34.0 40.0 27.0 41.0 39 35.18818657338145 38.0 34.0 40.0 26.0 41.0 40 35.03242294798672 38.0 34.0 40.0 26.0 41.0 41 35.06921256345037 38.0 34.0 40.0 26.0 41.0 42 35.050481858220316 38.0 34.0 40.0 26.0 41.0 43 35.084533467103036 38.0 34.0 40.0 26.0 41.0 44 35.06210567954053 38.0 34.0 40.0 26.0 41.0 45 34.883987191698026 37.0 34.0 40.0 26.0 41.0 46 34.85439506407466 37.0 33.0 40.0 26.0 41.0 47 34.754487656871866 37.0 33.0 40.0 26.0 41.0 48 34.799137715764424 37.0 34.0 40.0 26.0 41.0 49 34.71589701205916 37.0 33.0 40.0 26.0 41.0 50 34.62165566528698 37.0 33.0 40.0 26.0 41.0 51 33.41801338728319 36.0 32.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 4.0 11 4.0 12 14.0 13 10.0 14 28.0 15 35.0 16 75.0 17 164.0 18 351.0 19 661.0 20 1092.0 21 1732.0 22 2366.0 23 2865.0 24 3312.0 25 3796.0 26 4355.0 27 5076.0 28 6447.0 29 8332.0 30 11167.0 31 14864.0 32 19960.0 33 27234.0 34 41207.0 35 47435.0 36 55061.0 37 70414.0 38 79280.0 39 45177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.36386980436181 35.437186062905646 20.329754109772185 25.869190022960364 2 21.691906859159506 34.81710160236366 19.009146577270787 24.481844961206047 3 18.975114857055726 31.487517651192547 23.55746388549431 25.979903606257416 4 21.574342734780195 32.43753300966368 18.801420492841185 27.186703762714938 5 16.079545831224767 37.28793708109494 19.01312431080242 27.619392776877877 6 19.610889266528037 36.38764339176919 22.401269338966983 21.600198002735794 7 76.66860397022003 9.255743957712273 8.031264985558618 6.044387086509075 8 78.22787551461927 6.106704911837956 6.25609090447031 9.409328669072458 9 72.56358296557714 9.702797009628325 11.74845697086752 5.9851630539270175 10 30.521812343791087 37.54825764222055 14.705901851634959 17.224028162353406 11 22.183377935512098 28.162353403945467 31.005548938276622 18.648719722265806 12 25.73416806808112 22.59396843005487 33.760792364519496 17.911071137344507 13 23.850711240853975 30.00139220673607 26.760644304438046 19.387252247971908 14 15.670502233055409 32.84944941538367 32.0779900954435 19.40205825611742 15 13.951458391802332 30.353200639089188 33.0222598388134 22.67308113029508 16 14.562482459300053 29.183967965985957 32.460073499676255 23.793476075037734 17 13.8047242215244 27.635745681396802 30.205582528026447 28.35394756905235 18 18.272160947938097 27.344708177999156 29.19347032942263 25.189660544640113 19 19.438741798686905 33.42511585148911 27.88921570144016 19.246926648383823 20 19.959382920938125 31.52464316415443 26.07205443307353 22.443919481833912 21 17.341150316340308 31.685078416596873 27.830433639250508 23.143337627812315 22 16.074242186515924 30.176412482127823 26.678658796647213 27.070686534709036 23 17.17961013791686 30.978367759143815 23.64011234887375 28.201909754065575 24 14.774186277261286 33.27528788846435 27.753972761364714 24.196553072909648 25 16.64239512595051 31.371058452794248 25.83162253960607 26.154923881649168 26 19.22836389190288 32.788678486428196 23.688729092038123 24.2942285296308 27 16.600186953475987 30.065256928438366 25.870957904529973 27.463598213555674 28 18.853572999144788 31.455253812547095 27.05809037852554 22.63308280978257 29 17.918805619211568 32.83729522959257 25.31827392882951 23.925625222366353 30 21.378328865749282 29.619087817307115 24.85884570592616 24.14373761101744 31 20.961771770909067 34.13801409443582 19.360513039231503 25.539701095423617 32 21.856098859937372 28.999445327157535 21.93609550096239 27.2083603119427 33 19.604259710641983 29.959405019457748 22.766999838680807 27.669335431219462 34 16.09810858770571 32.807020257712935 23.319241843988873 27.775629310592482 35 17.602133833054523 29.211812100707373 27.088586335601377 26.097467730636726 36 20.778133072865447 29.52340122735178 26.346076076363644 23.352389623419125 37 18.560767614177525 29.955206300729913 24.002307085448347 27.48171899964421 38 18.555463969468686 28.19086049425549 25.97504193194098 27.278633604334846 39 17.451421929244958 26.28972485133221 26.447287296224026 29.811565923198806 40 19.90524154786871 28.020922878376375 25.59163261653102 26.482202957223894 41 16.954647208183523 25.96134084977647 31.224545267712518 25.859466674327486 42 22.410550717207453 27.612542235795623 26.231163774338757 23.745743272658164 43 18.575352637126837 26.86097158351362 28.634377783032317 25.92929799632723 44 20.89238241930173 24.845365608957856 26.581646295514666 27.68060567622575 45 21.85035324483613 27.232889668721093 26.725728643438174 24.191028443004605 46 18.758770349974256 25.646657930385242 30.716500301644796 24.87807141799571 47 18.619549676367182 28.121692127844355 25.695716643942024 27.56304155184644 48 19.390346040718732 23.985512210537017 32.64923682762492 23.974904921119332 49 20.805977207586864 22.604796704668754 30.374194232728353 26.21503185501603 50 18.856003836303007 22.82533993047806 28.102245430578606 30.216410802640333 51 18.205644403881386 23.303993865450952 32.00307611393113 26.487285616736532 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3968.0 1 3133.5 2 2299.0 3 1594.0 4 889.0 5 1109.5 6 1330.0 7 1292.5 8 1255.0 9 1242.0 10 1229.0 11 1295.0 12 1361.0 13 1394.5 14 1428.0 15 1407.0 16 1386.0 17 1395.0 18 1404.0 19 1433.0 20 1462.0 21 1446.0 22 1430.0 23 1734.5 24 2039.0 25 2324.0 26 2856.0 27 3103.0 28 4099.5 29 5096.0 30 5720.0 31 6344.0 32 7284.5 33 8225.0 34 9514.5 35 10804.0 36 12233.5 37 13663.0 38 14561.5 39 15460.0 40 17288.5 41 19117.0 42 22796.5 43 26476.0 44 36012.0 45 45548.0 46 48673.5 47 51799.0 48 53975.0 49 56151.0 50 52317.5 51 48484.0 52 42005.5 53 35527.0 54 29895.0 55 24263.0 56 20780.0 57 17297.0 58 14958.5 59 12620.0 60 10744.5 61 8869.0 62 7573.5 63 6278.0 64 5137.5 65 3997.0 66 3210.0 67 2423.0 68 2021.5 69 1620.0 70 1725.0 71 1830.0 72 1605.0 73 1380.0 74 1104.0 75 636.5 76 445.0 77 376.5 78 308.0 79 247.0 80 186.0 81 145.5 82 105.0 83 98.0 84 91.0 85 75.0 86 59.0 87 38.5 88 18.0 89 13.0 90 8.0 91 6.5 92 5.0 93 3.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 452519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.936575584175486 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88440803390097 32.29257531892727 2 8.928906556313446 7.310377162531309 3 3.275437925629269 4.0225563664139266 4 1.739292957936964 2.8480279054244275 5 1.0885379890203566 2.2280508831889105 6 0.7815766190739936 1.9197042204928123 7 0.5790402349661711 1.6592747041479976 8 0.4874792337159485 1.59645843973831 9 0.40301884653755443 1.4848390325818634 >10 3.600841868733777 30.280141173830867 >50 0.17332313427399904 4.483133906125371 >100 0.04944327654838205 3.6761875967672144 >500 0.0048899943839059165 1.513357962754364 >1k 0.0038033289652601573 4.68531532707535 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT 4497 0.993770427319074 No Hit AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT 4257 0.9407339802306643 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC 3504 0.7743321274907794 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3409 0.7533385338516173 No Hit AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT 3222 0.7120143021618982 No Hit AAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT 1113 0.2459565233724993 No Hit AACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTG 1063 0.23490726356241395 No Hit AATGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT 969 0.21413465511945354 No Hit AATGATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCT 885 0.1955718986385102 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 835 0.1845226388284249 No Hit AAAAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT 769 0.16993761587911227 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 755 0.16684382313228838 No Hit CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC 747 0.16507594156267472 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 714 0.1577834300880184 No Hit AATGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTC 580 0.12817141379698974 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTAT 550 0.12154185791093855 No Hit GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.10563092378441567 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2282357204890844 0.0 2 0.0 0.0 0.0 1.6772776391709519 0.0 3 0.0 0.0 0.0 2.3919437636872707 0.0 4 0.0 0.0 0.0 5.441097500878416 0.0 5 0.0 0.0 0.0 6.072452206426691 0.0 6 0.0 0.0 0.0 8.383736373500339 0.0 7 0.0 0.0 0.0 10.19802483431635 0.0 8 0.0 0.0 0.0 11.784256572652199 0.0 9 0.0 0.0 0.0 14.245147717554401 0.0 10 2.2098519620170645E-4 0.0 0.0 16.827801705563743 0.0 11 2.2098519620170645E-4 0.0 0.0 19.90811435541933 0.0 12 2.2098519620170645E-4 0.0 0.0 21.24198099969283 0.0 13 2.2098519620170645E-4 0.0 0.0 21.9502385535193 0.0 14 2.2098519620170645E-4 0.0 0.0 22.594631385643474 0.0 15 2.2098519620170645E-4 0.0 0.0 23.022016755097575 0.0 16 2.2098519620170645E-4 0.0 0.0 23.819773313385735 0.0 17 2.2098519620170645E-4 0.0 0.0 24.826581867280712 0.0 18 2.2098519620170645E-4 0.0 0.0 26.360661099312956 0.0 19 2.2098519620170645E-4 0.0 0.0 26.90605256353877 0.0 20 2.2098519620170645E-4 0.0 0.0 27.524811112903546 0.0 21 2.2098519620170645E-4 0.0 0.0 28.124343950198774 0.0 22 2.2098519620170645E-4 0.0 0.0 28.755919640943254 0.0 23 2.2098519620170645E-4 0.0 0.0 29.428377592985047 0.0 24 2.2098519620170645E-4 0.0 0.0 29.895319312559252 0.0 25 2.2098519620170645E-4 0.0 0.0 30.261933753057882 0.0 26 2.2098519620170645E-4 0.0 0.0 30.619266815316042 0.0 27 2.2098519620170645E-4 0.0 0.0 31.04643119957394 0.0 28 2.2098519620170645E-4 0.0 0.0 31.460336472059737 0.0 29 2.2098519620170645E-4 0.0 0.0 31.901201938482142 0.0 30 2.2098519620170645E-4 0.0 0.0 32.559958808359426 0.0 31 2.2098519620170645E-4 0.0 0.0 32.941379257003575 0.0 32 2.2098519620170645E-4 0.0 0.0 33.313739312603445 0.0 33 2.2098519620170645E-4 0.0 0.0 33.73007542224747 0.0 34 2.2098519620170645E-4 0.0 0.0 34.11348473765742 0.0 35 2.2098519620170645E-4 0.0 0.0 34.55810695241526 0.0 36 2.2098519620170645E-4 0.0 0.0 34.936654593508784 0.0 37 2.2098519620170645E-4 0.0 0.0 35.289567951842905 0.0 38 2.2098519620170645E-4 0.0 0.0 35.6510997328289 0.0 39 4.419703924034129E-4 0.0 0.0 36.023901758821175 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAAG 30 2.1625729E-6 45.000004 1 CATGACG 30 2.1625729E-6 45.000004 1 CGTTCGA 35 1.2097371E-7 45.000004 14 CCAGACG 30 2.1625729E-6 45.000004 1 GTTCGAA 35 1.2097371E-7 45.000004 15 CGTCGCC 35 1.2097371E-7 45.000004 27 CGCACCG 30 2.1625729E-6 45.000004 1 CCGGGTG 20 7.028749E-4 45.0 5 CGAACCG 20 7.028749E-4 45.0 1 CGACATG 20 7.028749E-4 45.0 1 TACGGGT 40 6.7993824E-9 45.0 4 CGCTGCC 20 7.028749E-4 45.0 25 CGTTCAT 50 2.1827873E-11 45.0 17 AATATGG 25 3.8870443E-5 45.0 2 CTGCGCG 25 3.8870443E-5 45.0 1 CTAACCG 20 7.028749E-4 45.0 1 AACGCGC 20 7.028749E-4 45.0 16 CGTACAT 45 3.8380676E-10 45.0 35 ACGTCAC 20 7.028749E-4 45.0 25 GGCGCAG 20 7.028749E-4 45.0 9 >>END_MODULE