FastQCFastQC Report
Sat 18 Jun 2016
SRR3548713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460818
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT47221.024699556006927No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT46141.0012629714985093No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC37410.8118172467221333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35290.7658120993537578No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT33640.7300062063547865No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT12110.26279355407123856No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTG10930.23718691544167111No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT9810.21288230928479357No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCT9100.19747492502463013No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT8290.17989748664331687No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8060.17490636216467237No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.1710002647466028No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC7730.1677451835648781No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7530.16340507532257856No Hit
AATGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTC5550.12043800372381287No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTAT5540.1202209983116979No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.10199254369403973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG302.1626602E-645.0000042
GACCGAT351.2098099E-745.0000049
CGAATCA302.1626602E-645.0000049
AACGTAA207.028884E-445.020
AAGTAAG207.028884E-445.01
CAGCGCG207.028884E-445.01
CCTTACG207.028884E-445.01
CACGGTA253.887156E-545.04
TAGACGG406.7993824E-945.02
CTATACG207.028884E-445.01
GAGTACG253.887156E-545.01
CGCATAG253.887156E-545.01
GAATCGG207.028884E-445.02
GGATCGA406.7993824E-945.08
ATTAACG253.887156E-545.01
TTCGTCG207.028884E-445.014
CGATTAG253.887156E-545.01
TTTCCCG207.028884E-445.015
TCTAGCG253.887156E-545.01
TAGCACG207.028884E-445.01