##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460818
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.082357894005877	31.0	31.0	33.0	28.0	34.0
2	31.370408707993178	31.0	31.0	34.0	30.0	34.0
3	31.553832532583364	31.0	31.0	34.0	30.0	34.0
4	35.49035627948561	37.0	35.0	37.0	33.0	37.0
5	34.77862192883091	35.0	35.0	37.0	32.0	37.0
6	34.852909391560225	35.0	35.0	37.0	32.0	37.0
7	34.92057167905767	35.0	35.0	37.0	32.0	37.0
8	35.27052111679665	37.0	35.0	37.0	33.0	37.0
9	36.749799269993794	39.0	37.0	39.0	32.0	39.0
10	36.05302961255854	37.0	35.0	39.0	31.0	39.0
11	36.32320786080405	38.0	35.0	39.0	32.0	39.0
12	36.19029204588362	37.0	35.0	39.0	32.0	39.0
13	35.995668571974186	37.0	35.0	39.0	31.0	39.0
14	36.98847701261669	39.0	36.0	40.0	32.0	41.0
15	37.00702229513604	38.0	36.0	40.0	32.0	41.0
16	36.85123844988694	38.0	35.0	40.0	32.0	41.0
17	36.80265961833088	38.0	35.0	40.0	32.0	41.0
18	36.67042737045862	38.0	35.0	40.0	31.0	41.0
19	36.736529389042964	38.0	35.0	40.0	31.0	41.0
20	36.64114032003958	38.0	35.0	40.0	31.0	41.0
21	36.78052289624103	38.0	35.0	40.0	31.0	41.0
22	36.77421888901909	39.0	35.0	40.0	31.0	41.0
23	36.667055106354354	38.0	35.0	40.0	31.0	41.0
24	36.22697681080166	38.0	35.0	40.0	30.0	41.0
25	36.364551731920194	38.0	35.0	40.0	30.0	41.0
26	36.25425222105039	38.0	35.0	40.0	30.0	41.0
27	36.14452777452269	38.0	35.0	40.0	30.0	41.0
28	35.98281317136049	38.0	35.0	40.0	29.0	41.0
29	35.558589291216926	38.0	34.0	40.0	27.0	41.0
30	35.6658594065336	38.0	34.0	40.0	28.0	41.0
31	35.493196880330196	38.0	34.0	40.0	27.0	41.0
32	35.5058070648282	38.0	34.0	40.0	27.0	41.0
33	35.41979045957406	38.0	34.0	40.0	27.0	41.0
34	35.23983872157772	38.0	34.0	40.0	26.0	41.0
35	35.26230095178574	38.0	34.0	40.0	27.0	41.0
36	35.20109240524459	38.0	34.0	40.0	26.0	41.0
37	35.169704742436274	38.0	34.0	40.0	26.0	41.0
38	35.19077813800676	38.0	34.0	40.0	27.0	41.0
39	35.163517918136876	38.0	34.0	40.0	26.0	41.0
40	34.950540126470756	38.0	33.0	40.0	26.0	41.0
41	35.0043813392706	38.0	34.0	40.0	26.0	41.0
42	35.00298816452482	38.0	34.0	40.0	26.0	41.0
43	35.06996037481175	38.0	34.0	40.0	26.0	41.0
44	34.98488557304619	38.0	34.0	40.0	26.0	41.0
45	34.80222777756077	37.0	33.0	40.0	25.0	41.0
46	34.75759193434284	37.0	33.0	40.0	26.0	41.0
47	34.654798640678095	37.0	33.0	40.0	25.0	41.0
48	34.68722358935632	37.0	33.0	40.0	26.0	41.0
49	34.57308091263796	37.0	33.0	40.0	25.0	41.0
50	34.51477372845679	37.0	33.0	40.0	25.0	41.0
51	33.23520131592082	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	6.0
12	10.0
13	13.0
14	22.0
15	48.0
16	94.0
17	160.0
18	339.0
19	623.0
20	1182.0
21	1780.0
22	2379.0
23	2989.0
24	3389.0
25	3811.0
26	4514.0
27	5306.0
28	6715.0
29	8598.0
30	11593.0
31	15349.0
32	20403.0
33	28224.0
34	41678.0
35	49307.0
36	55496.0
37	71161.0
38	79464.0
39	46155.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.31286972297089	35.49492424341063	20.303460368301586	25.888745665316893
2	21.685567838061882	34.92029391213017	19.066312513834095	24.327825735973853
3	18.855600258670453	31.662174654635887	23.723465663233643	25.75875942346002
4	21.568601920931908	32.47355789053379	18.87296069163965	27.084879496894654
5	16.072939859120087	37.330139013667	18.935241244916647	27.661679882296262
6	19.588210529970617	36.456041213667866	22.33419701487355	21.621551241487964
7	76.25049368731256	9.382011987378966	8.195643399346379	6.171850925962094
8	77.97850778398413	6.088737853122057	6.400574630331281	9.53217973256253
9	72.42881137455568	9.63807837367464	11.910776054754805	6.022334197014874
10	30.358623144061212	37.50569639206802	14.854237464682368	17.2814429991884
11	22.157771614824075	28.103502901362358	31.131379416602652	18.607346067210916
12	25.48424757713457	22.582885217157315	34.09306928114787	17.839797924560237
13	23.79486044381947	29.924612319831255	26.823170969883993	19.457356266465286
14	15.567534254304302	33.00326810150645	32.247221245697865	19.18197639849138
15	13.899630656788581	30.353198008758337	33.154303868338474	22.592867466114605
16	14.486630296559596	29.26296281829269	32.47898302583667	23.77142385931105
17	13.688050379976477	27.813366665364637	30.342998754388933	28.155584200269956
18	17.967397106883844	27.455307735374916	29.288569456922254	25.288725700818976
19	19.306537505045377	33.576379394902105	27.94313590180939	19.173947198243123
20	19.943882400427064	31.652409411090716	26.080578449626536	22.323129738855688
21	17.288170166963965	31.75331692772418	27.70963807837368	23.248874826938184
22	15.927546233003051	30.24664835140988	26.811669683041895	27.01413573254517
23	17.413382289754306	30.92609229674188	23.60498070821886	28.05554470528495
24	14.656979545069854	33.53319531789123	27.806422492176956	24.003402644861964
25	16.72439010628925	31.32060813596691	25.79977344634975	26.155228311394087
26	19.23557673528378	32.83074879887504	23.687225759410442	24.246448706430737
27	16.471144790351072	30.107330876832066	25.855326831851187	27.56619750096567
28	18.737983325304132	31.497901557664846	27.08791757266426	22.676197544366754
29	17.880594942037856	32.95682894331385	25.252702802407896	23.909873312240407
30	21.484186815619182	29.52293530200643	24.7355789053379	24.257298977036488
31	20.856390158370548	34.195712841078254	19.412870156981715	25.53502684356948
32	21.887165865916696	28.968920484876893	21.92991593210335	27.213997717103066
33	19.837115737666497	29.91701713040723	22.65493101397949	27.59093611794678
34	16.044078139308795	33.03039377802082	23.06008011839815	27.865447964272228
35	17.665976589456143	29.035758151808306	27.126110525196502	26.172154733539056
36	20.729876003107517	29.536823648381787	26.36290249078812	23.370397857722573
37	18.473236722523858	30.05980669157889	23.963473649032807	27.503482936864444
38	18.630782651719333	28.21026956412293	25.98618109535652	27.172766688801218
39	17.44463106909886	26.304094024104963	26.508730127729386	29.742544779066787
40	19.902000355888877	28.072037116605685	25.484898593370918	26.541063934134517
41	17.021904526298886	25.907191125346667	31.201689170127906	25.869215178226547
42	22.510405409510913	27.409302588006547	26.223585016210304	23.85670698627224
43	18.57739932033905	26.780420903697337	28.534909660646935	26.107270115316677
44	20.81494212465659	24.686101671375685	26.600523417054024	27.89843278691371
45	21.94402128389082	27.236349274550907	26.85246670051951	23.967162741038763
46	18.597146812841512	25.670438220729224	30.864028748876997	24.868386217552267
47	18.57653129869059	27.921435360597897	25.626820132894114	27.8752132078174
48	19.27528872570082	23.9662947193903	32.770855305131306	23.98756124977757
49	20.799100729572196	22.446822823761224	30.51139495419016	26.24268149247642
50	18.815237252017063	22.859133106779684	28.02255988264347	30.30306975855978
51	18.206754076446664	23.135163990989934	32.229643807316556	26.428438125246846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4073.0
1	3213.5
2	2354.0
3	1632.5
4	911.0
5	1104.5
6	1298.0
7	1268.0
8	1238.0
9	1264.5
10	1291.0
11	1330.5
12	1370.0
13	1412.0
14	1454.0
15	1442.0
16	1430.0
17	1414.5
18	1399.0
19	1442.5
20	1486.0
21	1472.5
22	1459.0
23	1718.0
24	1977.0
25	2346.0
26	2989.0
27	3263.0
28	4346.0
29	5429.0
30	6000.5
31	6572.0
32	7556.0
33	8540.0
34	9725.0
35	10910.0
36	12446.0
37	13982.0
38	14860.5
39	15739.0
40	17677.0
41	19615.0
42	23337.5
43	27060.0
44	36897.5
45	46735.0
46	49850.5
47	52966.0
48	55237.5
49	57509.0
50	53188.0
51	48867.0
52	42395.5
53	35924.0
54	30258.5
55	24593.0
56	21031.0
57	17469.0
58	15026.0
59	12583.0
60	10724.0
61	8865.0
62	7576.0
63	6287.0
64	5171.0
65	4055.0
66	3266.5
67	2478.0
68	2052.0
69	1626.0
70	1736.5
71	1847.0
72	1613.5
73	1380.0
74	1120.0
75	650.0
76	440.0
77	378.5
78	317.0
79	241.0
80	165.0
81	129.0
82	93.0
83	83.0
84	73.0
85	71.0
86	69.0
87	45.0
88	21.0
89	16.5
90	12.0
91	12.0
92	12.0
93	8.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.070864761986954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13464559996835	32.50128327424066
2	8.913531388259733	7.321728843978825
3	3.176217511344964	3.913499995893114
4	1.681721242298152	2.7627898283915235
5	1.0881114384933916	2.2344838868166588
6	0.7913286127124772	1.950033026100294
7	0.5892062973300023	1.6939448508186117
8	0.4686368083736501	1.53978551833627
9	0.37210311403169444	1.3754337006518937
>10	3.5306865011821036	29.574651702936954
>50	0.1960548012986029	5.068931087536848
>100	0.049278639245324087	3.7037668804219903
>500	0.004768900572128137	1.5122789853723726
>1k	0.0037091448894329953	4.84738841850399
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	4722	1.024699556006927	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT	4614	1.0012629714985093	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC	3741	0.8118172467221333	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3529	0.7658120993537578	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT	3364	0.7300062063547865	No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT	1211	0.26279355407123856	No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTG	1093	0.23718691544167111	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT	981	0.21288230928479357	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCT	910	0.19747492502463013	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTT	829	0.17989748664331687	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	806	0.17490636216467237	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	788	0.1710002647466028	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	773	0.1677451835648781	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	753	0.16340507532257856	No Hit
AATGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTC	555	0.12043800372381287	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTAT	554	0.1202209983116979	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.10199254369403973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1700541211497814E-4	0.0	0.0	1.2701326771089672	0.0
2	2.1700541211497814E-4	0.0	0.0	1.7462425512892292	0.0
3	2.1700541211497814E-4	0.0	0.0	2.4693045844563364	0.0
4	2.1700541211497814E-4	0.0	0.0	5.660586175019205	0.0
5	2.1700541211497814E-4	0.0	0.0	6.291203902625331	0.0
6	2.1700541211497814E-4	0.0	0.0	8.614463844728288	0.0
7	2.1700541211497814E-4	0.0	0.0	10.493730713643998	0.0
8	2.1700541211497814E-4	0.0	0.0	12.087852471040629	0.0
9	2.1700541211497814E-4	0.0	0.0	14.59274594308382	0.0
10	2.1700541211497814E-4	0.0	0.0	17.181403504203395	0.0
11	2.1700541211497814E-4	0.0	0.0	20.272211588957028	0.0
12	2.1700541211497814E-4	0.0	0.0	21.60744588970049	0.0
13	2.1700541211497814E-4	0.0	0.0	22.324865782152607	0.0
14	2.1700541211497814E-4	0.0	0.0	22.990421381109243	0.0
15	2.1700541211497814E-4	0.0	0.0	23.43398044347226	0.0
16	2.1700541211497814E-4	0.0	0.0	24.232777365467495	0.0
17	2.1700541211497814E-4	0.0	0.0	25.210386747045472	0.0
18	2.1700541211497814E-4	0.0	0.0	26.76566453567352	0.0
19	2.1700541211497814E-4	0.0	0.0	27.325538498930165	0.0
20	2.1700541211497814E-4	0.0	0.0	27.94747601005169	0.0
21	2.1700541211497814E-4	0.0	0.0	28.563771380458228	0.0
22	2.1700541211497814E-4	0.0	0.0	29.19026600523417	0.0
23	2.1700541211497814E-4	0.0	0.0	29.892712524250356	0.0
24	2.1700541211497814E-4	0.0	0.0	30.33583757578914	0.0
25	2.1700541211497814E-4	0.0	0.0	30.704963781796717	0.0
26	2.1700541211497814E-4	0.0	0.0	31.05325746824126	0.0
27	2.1700541211497814E-4	0.0	0.0	31.469473848677787	0.0
28	2.1700541211497814E-4	0.0	0.0	31.872235893563186	0.0
29	2.1700541211497814E-4	0.0	0.0	32.27998906292723	0.0
30	2.1700541211497814E-4	0.0	0.0	32.94424262941118	0.0
31	2.1700541211497814E-4	0.0	0.0	33.33268231709699	0.0
32	2.1700541211497814E-4	0.0	0.0	33.71265879371032	0.0
33	2.1700541211497814E-4	0.0	0.0	34.110646719529186	0.0
34	2.1700541211497814E-4	0.0	0.0	34.48693410413656	0.0
35	2.1700541211497814E-4	0.0	0.0	34.94047541545686	0.0
36	2.1700541211497814E-4	0.0	0.0	35.304610496985795	0.0
37	2.1700541211497814E-4	0.0	0.0	35.66961360016319	0.0
38	2.1700541211497814E-4	0.0	0.0	36.02289841108637	0.0
39	2.1700541211497814E-4	0.0	0.0	36.36793701634919	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.1626602E-6	45.000004	2
GACCGAT	35	1.2098099E-7	45.000004	9
CGAATCA	30	2.1626602E-6	45.000004	9
AACGTAA	20	7.028884E-4	45.0	20
AAGTAAG	20	7.028884E-4	45.0	1
CAGCGCG	20	7.028884E-4	45.0	1
CCTTACG	20	7.028884E-4	45.0	1
CACGGTA	25	3.887156E-5	45.0	4
TAGACGG	40	6.7993824E-9	45.0	2
CTATACG	20	7.028884E-4	45.0	1
GAGTACG	25	3.887156E-5	45.0	1
CGCATAG	25	3.887156E-5	45.0	1
GAATCGG	20	7.028884E-4	45.0	2
GGATCGA	40	6.7993824E-9	45.0	8
ATTAACG	25	3.887156E-5	45.0	1
TTCGTCG	20	7.028884E-4	45.0	14
CGATTAG	25	3.887156E-5	45.0	1
TTTCCCG	20	7.028884E-4	45.0	15
TCTAGCG	25	3.887156E-5	45.0	1
TAGCACG	20	7.028884E-4	45.0	1
>>END_MODULE