FastQCFastQC Report
Sat 18 Jun 2016
SRR3548711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701710
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC94991.353693121089909No Hit
AATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT91681.3065226375568255No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGT78251.1151330321642843No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT47740.6803380313804848TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29390.4188339912499466No Hit
AATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT20470.29171595103390285No Hit
AACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG18640.26563680152769664No Hit
AATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT17290.24639808467885596No Hit
AAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT13790.19651992988556524No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTAT12150.17314845163956621No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCTC10430.14863690128400622No Hit
AAAAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT8810.1255504410653974No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8370.11928004446281226No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.11514728306565389No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC8060.11486226503826366TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTCTTCCCTCGTATGCCGT7870.11215459377805646No Hit
AATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC7750.11044448561371507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG351.2110286E-745.0000041
GGATCGA302.1642882E-645.0000048
GTATACG302.1642882E-645.0000041
AGTAACG302.1642882E-645.0000041
TAATACG207.031407E-445.01
AGTTTCG207.031407E-445.01
TACCACG207.031407E-445.01
TATGCGT253.889248E-545.018
CCCAACG253.889248E-545.01
TGTTCGA207.031407E-445.033
ACTACCG453.8380676E-1045.01
TATTACG253.889248E-545.01
TCGAATA253.889248E-545.045
GTAACGA207.031407E-445.09
ATAACGG1400.041.7857172
CGATAGG700.041.7857172
TAGGGCG1250.041.45
CGTGCGA556.002665E-1140.90909245
TTACGCG451.9264917E-840.01
TTTGCGC1300.039.80769313