SRR3548709_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416572
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT	5642	1.3543877168892773	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	4662	1.1191342673055318	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGT	4383	1.052159050536282	No Hit
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT	3761	0.902845126412721	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3239	0.7775366563283178	No Hit
AACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTG	1405	0.3372766292501656	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCT	1308	0.31399133883218266	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT	1213	0.29118615749498283	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT	996	0.23909432223001068	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTAT	779	0.18700248696503846	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	755	0.18124117799564063	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT	690	0.1656376328701881	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	680	0.16323708746627233	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	596	0.14307250607337987	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCATACTC	588	0.14115206975024724	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCCTACTCGTATGCC	524	0.12578857916518632	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	474	0.11378585214560748	TruSeq Adapter, Index 20 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	435	0.10442372507033597	No Hit
GGCACGGGGAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTC	418	0.10034279788367917	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2094279E-7	45.0	1
CTCGTTG	20	7.0281024E-4	45.0	15
CCTAGCG	20	7.0281024E-4	45.0	1
ACATTAG	40	6.7975634E-9	45.0	1
CACGCGA	20	7.0281024E-4	45.0	22
CGTTCTA	20	7.0281024E-4	45.0	27
TGAAACG	20	7.0281024E-4	45.0	1
TAACGAA	20	7.0281024E-4	45.0	23
TCTAGCG	25	3.8865088E-5	45.0	1
CACCTTG	20	7.0281024E-4	45.0	1
CCCGTTC	20	7.0281024E-4	45.0	16
CTAACGG	40	6.7975634E-9	45.0	2
ATATACG	20	7.0281024E-4	45.0	1
CCTCATG	20	7.0281024E-4	45.0	1
ACCAGTC	25	3.8865088E-5	45.0	29
AGATTCG	20	7.0281024E-4	45.0	14
CGGGACG	25	3.8865088E-5	45.0	6
CGTACTC	20	7.0281024E-4	45.0	45
ACGCTAG	20	7.0281024E-4	45.0	1
CAAGCCG	25	3.8865088E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content