##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416572
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046368935022038	31.0	31.0	33.0	28.0	34.0
2	31.35493504124137	31.0	31.0	34.0	30.0	34.0
3	31.514784959142716	31.0	31.0	34.0	30.0	34.0
4	35.46177131444264	37.0	35.0	37.0	33.0	37.0
5	34.73518623431244	35.0	35.0	37.0	32.0	37.0
6	34.84578416216164	35.0	35.0	37.0	32.0	37.0
7	34.871674044342875	35.0	35.0	37.0	32.0	37.0
8	35.2407410963771	37.0	35.0	37.0	33.0	37.0
9	36.68742018186532	39.0	37.0	39.0	32.0	39.0
10	36.03491353235455	37.0	35.0	39.0	31.0	39.0
11	36.30145809127834	37.0	35.0	39.0	32.0	39.0
12	36.04445089924431	37.0	35.0	39.0	32.0	39.0
13	35.85040281151878	37.0	35.0	39.0	31.0	39.0
14	36.79523587759139	38.0	35.0	40.0	31.0	41.0
15	36.89632284455028	38.0	35.0	40.0	32.0	41.0
16	36.706987027452634	38.0	35.0	40.0	31.0	41.0
17	36.64512497239373	38.0	35.0	40.0	31.0	41.0
18	36.502520572674115	38.0	35.0	40.0	31.0	41.0
19	36.56440423264166	38.0	35.0	40.0	31.0	41.0
20	36.50673833094879	38.0	35.0	40.0	31.0	41.0
21	36.64458004858704	38.0	35.0	40.0	31.0	41.0
22	36.61293365852722	38.0	35.0	40.0	31.0	41.0
23	36.46267872060532	38.0	35.0	40.0	31.0	41.0
24	36.1167265202654	38.0	34.0	40.0	30.0	41.0
25	36.22864714863217	38.0	35.0	40.0	30.0	41.0
26	36.1600659669877	38.0	35.0	40.0	30.0	41.0
27	36.01494579568478	38.0	34.0	40.0	30.0	41.0
28	35.88406565971789	38.0	34.0	40.0	29.0	41.0
29	35.39414074877812	38.0	34.0	40.0	27.0	41.0
30	35.60299540055501	38.0	34.0	40.0	28.0	41.0
31	35.40837598302334	38.0	34.0	40.0	27.0	41.0
32	35.24879252566183	38.0	34.0	40.0	27.0	41.0
33	35.24315124396263	38.0	34.0	40.0	27.0	41.0
34	35.06423619446338	38.0	34.0	40.0	26.0	41.0
35	35.087329441249054	38.0	34.0	40.0	26.0	41.0
36	34.97712040175528	38.0	34.0	40.0	25.0	41.0
37	34.85901836897343	38.0	33.0	40.0	25.0	41.0
38	34.72930009698204	37.0	33.0	40.0	25.0	41.0
39	34.61201424963752	37.0	33.0	40.0	24.0	41.0
40	34.459702044304464	37.0	33.0	40.0	24.0	41.0
41	34.63126182268611	37.0	33.0	40.0	24.0	41.0
42	34.654957606368164	37.0	33.0	40.0	24.0	41.0
43	34.67444043286635	37.0	33.0	40.0	25.0	41.0
44	34.60494464342298	37.0	33.0	40.0	24.0	41.0
45	34.33664768635434	37.0	33.0	40.0	24.0	41.0
46	34.19841227926985	37.0	33.0	40.0	23.0	41.0
47	34.16149669204843	37.0	33.0	40.0	23.0	41.0
48	34.17966882075608	37.0	33.0	40.0	23.0	41.0
49	34.06604380515253	37.0	33.0	40.0	23.0	41.0
50	33.9963823780763	36.0	33.0	40.0	23.0	41.0
51	32.6294734163602	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	6.0
12	9.0
13	12.0
14	16.0
15	43.0
16	59.0
17	146.0
18	318.0
19	672.0
20	1147.0
21	1801.0
22	2410.0
23	3050.0
24	3530.0
25	4135.0
26	4750.0
27	5872.0
28	7111.0
29	9053.0
30	11505.0
31	15090.0
32	19531.0
33	26344.0
34	39115.0
35	42813.0
36	46844.0
37	60077.0
38	69957.0
39	41148.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.2715785026358	37.03513438253171	19.55460280575747	24.138684309075025
2	22.22208885858867	34.93513726318619	18.82555716658825	24.017216711636884
3	18.15916576246123	30.396906177083434	25.581892205909185	25.86203585454615
4	22.407651018311363	32.10105335932324	18.541812699845405	26.949482922519998
5	16.42957279893992	37.864282765044216	19.24493244865233	26.46121198736353
6	19.892359544088418	34.546008853211454	24.047223529185832	21.514408073514304
7	75.63830502290119	10.446453434220254	7.835860307461856	6.079381235416687
8	76.79224719856352	5.884697003159118	6.19100659669877	11.1320492015786
9	72.8313472821025	9.26898591359957	11.749469479465734	6.1501973248322015
10	33.15753339158657	35.73403877360937	14.23019309987229	16.878234734931777
11	25.35936164696619	27.08175297427576	29.56415697646505	17.994728402293
12	26.034395014547307	23.340022853192245	33.88561881259422	16.73996331966623
13	24.062106910690108	30.442996648838616	26.480176296054463	19.01472014441681
14	15.301316459099507	33.293644315988594	32.4697771333647	18.9352620915472
15	14.227072390847201	29.14406153077979	34.21545375109225	22.413412327280756
16	15.772543521888174	28.37900771055184	33.3190900972701	22.529358670289888
17	14.801522906004244	27.111759791824703	30.011858694295345	28.074858607875708
18	18.959267545586357	26.919236050430655	29.33274439952757	24.78875200445541
19	20.42095964203067	32.10945526823694	28.672594413450735	18.796990676281652
20	20.94475864916509	30.883016621376374	25.751370711425636	22.420854018032895
21	18.420345102407268	30.620637008728384	28.25489951316939	22.704118375694957
22	17.520380630479245	30.751466733241795	26.131857157946285	25.596295478332674
23	18.53965220898188	30.674169171235704	23.6804201914675	27.105758428314914
24	14.764554506783941	32.66230087475874	28.044851790326764	24.528292828130553
25	18.11187501800409	29.05860211440039	26.915155123244	25.91436774435152
26	20.06015766782213	30.19982139942195	25.699038821620274	24.04098211113565
27	18.204296016054847	28.904006990388215	26.54859184006606	26.343105153490875
28	22.430696254188952	27.145607482019912	27.974995919072814	22.44870034471832
29	19.396406863639417	30.050747529838777	28.687237740414623	21.86560786610718
30	25.543723533986924	25.519237970866982	26.539709822071572	22.397328673074522
31	23.85950087859962	29.715151282371355	22.007960208559386	24.41738763046964
32	25.134670597159676	27.493686565587698	23.50181961341617	23.869823223836455
33	24.410426048798286	25.43641915443189	26.270608682292618	23.88254611447721
34	20.018628232334386	28.46470718147163	26.673420201069682	24.8432443851243
35	22.215847440538493	24.72753809665556	28.27602431272385	24.780590150082098
36	22.563446415025492	25.611658968917737	30.80883976839538	21.01605484766139
37	20.730870053676195	29.248725310390522	24.2419077614434	25.778496874489885
38	21.069106901087927	29.109733731503795	26.71999078190565	23.101168585502627
39	18.74969993182451	28.52688130743305	28.227773350105146	24.495645410637295
40	21.386939112566374	27.055587029373072	26.74471639956598	24.81275745849457
41	18.097471745580595	24.655041625457304	30.476844339033825	26.77064228992827
42	24.464918429467176	24.79427325888442	25.473147499111796	25.267660812536608
43	20.19002717417397	24.30696254188952	29.736516136466207	25.766494147470304
44	22.63234206811788	24.139404472696196	26.28933293644316	26.938920522742766
45	23.5978414295728	26.225958537779782	27.863850666871514	22.312349365775905
46	19.55460280575747	25.395609882565317	32.67406354723793	22.375723764439282
47	20.737111471726376	25.861075636384584	27.406066658344773	25.99574623354426
48	20.634848237519567	23.330900780657366	33.41727240429025	22.616978577532816
49	21.85168470276447	21.03934013807937	29.845500897803984	27.263474261352176
50	19.326791046925862	23.854219678711004	27.9012991751726	28.917690099190533
51	18.861565347646987	22.617938795694382	32.78640907214119	25.73408678451744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3720.0
1	2976.0
2	2232.0
3	1553.0
4	874.0
5	1009.5
6	1145.0
7	1100.5
8	1056.0
9	1055.5
10	1055.0
11	1077.5
12	1100.0
13	1160.0
14	1220.0
15	1093.0
16	966.0
17	965.5
18	965.0
19	1003.5
20	1042.0
21	1033.0
22	1024.0
23	1313.0
24	1602.0
25	1841.5
26	2619.0
27	3157.0
28	3644.0
29	4131.0
30	5209.0
31	6287.0
32	7228.5
33	8170.0
34	8836.5
35	9503.0
36	10776.0
37	12049.0
38	12174.5
39	12300.0
40	13903.0
41	15506.0
42	19259.0
43	23012.0
44	34112.5
45	45213.0
46	46970.5
47	48728.0
48	48400.0
49	48072.0
50	45738.0
51	43404.0
52	38268.0
53	33132.0
54	28673.5
55	24215.0
56	20667.0
57	17119.0
58	14780.5
59	12442.0
60	10762.0
61	9082.0
62	7809.0
63	6536.0
64	5622.0
65	4708.0
66	3839.5
67	2971.0
68	2567.0
69	2163.0
70	1853.5
71	1544.0
72	1385.5
73	1227.0
74	1080.0
75	623.0
76	313.0
77	325.0
78	337.0
79	229.5
80	122.0
81	81.5
82	41.0
83	39.0
84	37.0
85	30.0
86	23.0
87	14.5
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	416572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.04702394344067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34816000501684	29.396131835738203
2	8.59458474934565	6.368075739858766
3	3.044388964521182	3.383566525852883
4	1.6089516153021641	2.384274760637473
5	1.0114517332670607	1.873563828998968
6	0.7401116692941389	1.6451360839895883
7	0.5601555327693045	1.4526466804188636
8	0.43271573577250116	1.2824664179093919
9	0.38831827974377714	1.2947432946609063
>10	3.8498954149009377	31.63350347675446
>50	0.3660927476694792	8.648351579771967
>100	0.044787942534032024	3.129749077153046
>500	0.005192804931481974	1.3485695600979086
>1k	0.0045437043150467265	4.802475514392891
>5k	6.491006164352467E-4	1.3567456237646935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT	5642	1.3543877168892773	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	4662	1.1191342673055318	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGT	4383	1.052159050536282	No Hit
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT	3761	0.902845126412721	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3239	0.7775366563283178	No Hit
AACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTG	1405	0.3372766292501656	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCT	1308	0.31399133883218266	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT	1213	0.29118615749498283	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT	996	0.23909432223001068	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTAT	779	0.18700248696503846	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	755	0.18124117799564063	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT	690	0.1656376328701881	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	680	0.16323708746627233	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	596	0.14307250607337987	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCATACTC	588	0.14115206975024724	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCCTACTCGTATGCC	524	0.12578857916518632	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	474	0.11378585214560748	TruSeq Adapter, Index 20 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	435	0.10442372507033597	No Hit
GGCACGGGGAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTC	418	0.10034279788367917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1901904112614385	0.0
2	0.0	0.0	0.0	1.5615547852472083	0.0
3	0.0	0.0	0.0	2.367657931882124	0.0
4	0.0	0.0	0.0	5.644402408227149	0.0
5	0.0	0.0	0.0	6.198208232910518	0.0
6	0.0	0.0	0.0	8.29652497047329	0.0
7	0.0	0.0	0.0	10.116138386641445	0.0
8	0.0	0.0	0.0	11.866616095176823	0.0
9	0.0	0.0	0.0	14.639246036699538	0.0
10	0.0	0.0	0.0	17.195106728248657	0.0
11	0.0	0.0	0.0	20.418799151167146	0.0
12	0.0	0.0	0.0	21.58522416292982	0.0
13	0.0	0.0	0.0	22.247054530789395	0.0
14	0.0	0.0	0.0	22.939371825278702	0.0
15	0.0	0.0	0.0	23.335221762384414	0.0
16	0.0	0.0	0.0	24.041462220216435	0.0
17	0.0	0.0	0.0	24.77170813208761	0.0
18	0.0	0.0	0.0	26.283811682014154	0.0
19	0.0	0.0	0.0	26.717830291042123	0.0
20	0.0	0.0	0.0	27.332609968984954	0.0
21	0.0	0.0	0.0	27.824721776787687	0.0
22	0.0	0.0	0.0	28.313472821024938	0.0
23	0.0	0.0	0.0	28.81182604687785	0.0
24	0.0	0.0	0.0	29.149102676128017	0.0
25	0.0	0.0	0.0	29.40067983445839	0.0
26	0.0	0.0	0.0	29.66690031975265	0.0
27	0.0	0.0	0.0	30.043545893627034	0.0
28	0.0	0.0	0.0	30.344094178197288	0.0
29	0.0	0.0	0.0	30.67656971663962	0.0
30	0.0	0.0	0.0	31.298310976253806	0.0
31	0.0	0.0	0.0	31.60966171514168	0.0
32	0.0	0.0	0.0	31.940696926341666	0.0
33	0.0	0.0	0.0	32.229722592973125	0.0
34	0.0	0.0	0.0	32.51274689609479	0.0
35	0.0	0.0	0.0	32.827938507628936	0.0
36	0.0	0.0	0.0	33.09896008373102	0.0
37	0.0	0.0	0.0	33.3788636778276	0.0
38	0.0	0.0	0.0	33.66332830819162	0.0
39	0.0	0.0	0.0	33.95835533833287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2094279E-7	45.0	1
CTCGTTG	20	7.0281024E-4	45.0	15
CCTAGCG	20	7.0281024E-4	45.0	1
ACATTAG	40	6.7975634E-9	45.0	1
CACGCGA	20	7.0281024E-4	45.0	22
CGTTCTA	20	7.0281024E-4	45.0	27
TGAAACG	20	7.0281024E-4	45.0	1
TAACGAA	20	7.0281024E-4	45.0	23
TCTAGCG	25	3.8865088E-5	45.0	1
CACCTTG	20	7.0281024E-4	45.0	1
CCCGTTC	20	7.0281024E-4	45.0	16
CTAACGG	40	6.7975634E-9	45.0	2
ATATACG	20	7.0281024E-4	45.0	1
CCTCATG	20	7.0281024E-4	45.0	1
ACCAGTC	25	3.8865088E-5	45.0	29
AGATTCG	20	7.0281024E-4	45.0	14
CGGGACG	25	3.8865088E-5	45.0	6
CGTACTC	20	7.0281024E-4	45.0	45
ACGCTAG	20	7.0281024E-4	45.0	1
CAAGCCG	25	3.8865088E-5	45.0	1
>>END_MODULE