FastQCFastQC Report
Sat 18 Jun 2016
SRR3548703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592463
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC209033.528152812918275No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGT185623.133022652891404No Hit
AATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT172752.9157938976779985Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT63921.0788859388687564Illumina Single End Adapter 1 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT45690.7711873990443285No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT39290.6631637756281827No Hit
AACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG36000.6076328817158202Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTAT33350.5629043501450723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32870.5548025783888615No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGTC31660.5343793620867464No Hit
AATGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC15570.2628012213420922No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.18634075039285153No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTTGAGAGGTCGTATGCCGT10320.17418809275853514No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATG9210.15545274557229735No Hit
AAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT8690.14667582616973548Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC8070.13621103765129638No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA7930.1338480208890682No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.1303034957457259No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC6830.11528146061441812Illumina Single End Adapter 1 (95% over 23bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.10836119723932128No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGCGGTCGTATGCC5960.10059699930628578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACG253.8885082E-545.0000041
CCTAACG351.210592E-745.0000041
TCGTAGC253.8885082E-545.0000048
CGCACCG253.8885082E-545.0000041
TTCGCGG351.210592E-745.0000042
CGATCGA253.8885082E-545.00000418
CGATCAA351.210592E-745.00000418
CAATACG351.210592E-745.0000041
ACGCATG207.030516E-445.01
CCGATCA406.8048394E-945.017
CCTACCG453.8380676E-1045.01
TCTAGCG453.8380676E-1045.01
GCACCGT207.030516E-445.024
TAGCAAC207.030516E-445.011
ATTCACG207.030516E-445.01
CGTAGTG207.030516E-445.028
CGTAGCA207.030516E-445.09
TAACACG207.030516E-445.01
GGTATGC453.8380676E-1045.08
ACTCGCG207.030516E-445.01
Produced by FastQC (version 0.11.5)