##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592463
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125916723913562	31.0	31.0	33.0	30.0	34.0
2	31.411345518623104	31.0	31.0	34.0	30.0	34.0
3	31.530016220422205	31.0	31.0	34.0	30.0	34.0
4	35.411905891169575	37.0	35.0	37.0	33.0	37.0
5	34.67092290995387	35.0	35.0	37.0	32.0	37.0
6	34.82214923126001	35.0	35.0	37.0	32.0	37.0
7	34.63642286522534	35.0	35.0	37.0	31.0	37.0
8	35.09104534797954	36.0	35.0	37.0	32.0	37.0
9	36.686979608853214	38.0	35.0	39.0	32.0	39.0
10	36.09138460967183	37.0	35.0	39.0	32.0	39.0
11	36.27782494434251	37.0	35.0	39.0	32.0	39.0
12	35.974447349454735	37.0	35.0	39.0	31.0	39.0
13	35.96587803795342	37.0	35.0	39.0	31.0	39.0
14	36.91711887493396	39.0	36.0	40.0	31.0	41.0
15	37.0010093457313	38.0	36.0	40.0	32.0	41.0
16	36.65752629278115	38.0	35.0	40.0	31.0	41.0
17	36.66147928225054	38.0	35.0	40.0	31.0	41.0
18	36.37365371339645	38.0	35.0	40.0	30.0	41.0
19	36.40621777224907	38.0	35.0	40.0	30.0	41.0
20	36.544754693542046	38.0	35.0	40.0	30.0	41.0
21	36.82607521482354	39.0	35.0	40.0	31.0	41.0
22	36.74231302207902	39.0	35.0	40.0	31.0	41.0
23	36.68232952943897	38.0	35.0	40.0	31.0	41.0
24	36.222906746919215	38.0	35.0	40.0	30.0	41.0
25	36.44672156742278	38.0	35.0	40.0	30.0	41.0
26	36.29704302209589	38.0	35.0	40.0	30.0	41.0
27	36.24036437718473	38.0	35.0	40.0	30.0	41.0
28	36.20836744235505	38.0	35.0	40.0	30.0	41.0
29	35.92049630103483	38.0	34.0	40.0	29.0	41.0
30	36.0018279622525	38.0	35.0	40.0	30.0	41.0
31	35.76617273990106	38.0	34.0	40.0	29.0	41.0
32	35.88382565662328	38.0	35.0	40.0	29.0	41.0
33	35.58350310483524	38.0	34.0	40.0	28.0	41.0
34	35.78104624255017	38.0	35.0	40.0	29.0	41.0
35	35.690925846846135	38.0	34.0	40.0	29.0	41.0
36	35.45717622872652	38.0	34.0	40.0	28.0	41.0
37	35.53690779002233	38.0	34.0	40.0	28.0	41.0
38	35.20230799222905	38.0	34.0	40.0	27.0	41.0
39	35.26478109181502	38.0	34.0	40.0	27.0	41.0
40	34.82775126885561	37.0	33.0	40.0	25.0	41.0
41	35.00500790766681	37.0	33.0	40.0	26.0	41.0
42	35.21385301698165	38.0	34.0	40.0	27.0	41.0
43	35.12272665128455	38.0	34.0	40.0	27.0	41.0
44	35.128841801091376	37.0	34.0	40.0	27.0	41.0
45	34.92656925411376	37.0	33.0	40.0	26.0	41.0
46	34.82021830899145	37.0	33.0	40.0	26.0	41.0
47	34.735932876820996	37.0	33.0	40.0	26.0	41.0
48	34.88229813507341	37.0	33.0	40.0	26.0	41.0
49	34.75519146343316	37.0	33.0	40.0	26.0	41.0
50	34.62730162052314	37.0	33.0	40.0	26.0	41.0
51	33.21745324180582	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	5.0
11	12.0
12	5.0
13	19.0
14	28.0
15	52.0
16	71.0
17	189.0
18	386.0
19	727.0
20	1231.0
21	1894.0
22	2663.0
23	3278.0
24	3775.0
25	4414.0
26	5170.0
27	6442.0
28	8444.0
29	11273.0
30	15126.0
31	20794.0
32	28009.0
33	37842.0
34	53234.0
35	62158.0
36	73191.0
37	91459.0
38	101786.0
39	58779.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.796829506652735	44.2275720171555	16.778431733289672	22.197166742902088
2	19.73389055519079	42.84183147302026	16.94283018517612	20.481447786612836
3	17.68346715322307	26.349493554871785	33.197516131809074	22.76952316009607
4	29.663793350808405	27.363565319690853	17.94069840648277	25.03194292301798
5	15.730433799241473	40.95394986691152	18.661249732050777	24.65436660179623
6	19.586370794463114	30.170491659394767	30.43970678337719	19.803430762764933
7	67.11541480227457	16.58027589908568	9.694107480129562	6.610201818510185
8	68.84193612090544	4.9981180259357965	7.043984181290646	19.115961671868117
9	70.21332977755573	7.76791124509041	13.198123764690791	8.820635212663069
10	34.38577598938668	30.84935261780061	16.69251919529152	18.072352197521198
11	24.290124446589914	23.833387063833523	28.572248393570572	23.304240096005994
12	23.80975689621124	21.672914595510605	37.59019550587969	16.927133002398463
13	23.989346170140582	29.93739693449211	23.906471796551006	22.166785098816298
14	14.838226184588743	27.835155950666962	36.963658490066045	20.36295937467825
15	13.852173047093236	28.642970109525827	28.673520540523207	28.83133630285773
16	16.091300216216034	27.724262949753825	33.909965685620875	22.274471148409265
17	15.444339984100273	26.141716866707288	29.823296982258807	28.59064616693363
18	17.23786970663147	26.702426987001722	28.040400835157637	28.019302471209173
19	18.70479675524041	29.754094348507838	32.567097017028914	18.97401187922284
20	20.311141792820816	33.126794415853816	25.835031048352384	20.727032742972977
21	17.831155700862332	26.815851791588674	29.429179543701462	25.92381296384753
22	17.07431518930296	31.97870584323409	26.88792380283663	24.059055164626315
23	20.35705183277268	27.144142334626803	22.704540199134797	29.794265633465717
24	14.696951539589815	36.15381888826813	25.93292745707327	23.21630211506879
25	18.250759963069424	24.777243473432094	28.862393094589873	28.109603468908606
26	21.889974563812423	29.85300347869825	23.57801246660129	24.67900949088804
27	17.26453803866233	26.195391104592186	30.714154301618834	25.82591655512665
28	21.368423007006346	24.985188948508178	30.624190877742574	23.022197166742902
29	19.574386923740388	29.284697947382366	30.966659521354078	20.17425560752317
30	25.90760942033511	24.73032071201071	25.39112147087666	23.97094839677752
31	24.87362079994869	33.05505997842903	22.843283040459912	19.228036181162366
32	26.950037386300917	23.169716927470578	22.979831651934383	26.900414034294123
33	25.035318661249732	28.843826534315227	26.36941041043913	19.75144439399591
34	24.678334343241687	24.221765747396883	28.08411664525886	23.01578326410257
35	28.045126868682097	23.930945898731228	28.770741801597737	19.25318543098894
36	23.01797749395321	27.968160036998093	32.18597617066382	16.82788629838488
37	31.83017336103689	24.883241653909188	21.54986893696315	21.736716048090766
38	25.283941782018456	32.61503249991983	24.669726210750714	17.431299507311003
39	28.952694092289306	23.21090093389798	26.963033978493172	20.87337099531954
40	28.135090292558353	28.980206358878107	22.754332338053178	20.130371010510363
41	25.20646858959969	21.325888705286236	32.24623309810064	21.221409607013435
42	30.353119097732684	22.478872098342006	24.303289825693756	22.86471897823155
43	23.054266679944572	22.8461524179569	30.436837405880198	23.66274349621833
44	23.69599451780111	21.131614970048762	26.92674479250181	28.245645719648316
45	28.929063924667027	26.778549884127784	25.08612352163764	19.20626266956755
46	20.90746595146026	21.7225379475174	36.49645631879122	20.873539782231127
47	23.459692841578292	27.537584625537797	23.983607415146597	25.01911511773731
48	22.190752840261755	20.86054319003887	35.011469070642384	21.937234899056985
49	25.79891064927261	20.025891912237554	29.374998945081803	24.800198493408026
50	21.99107792385347	21.07760315834069	26.467475606071606	30.463843311734234
51	19.161027777262042	19.947237211437677	35.27832117786258	25.613413833437697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3783.0
1	2969.5
2	2156.0
3	1478.5
4	801.0
5	1084.0
6	1367.0
7	1300.0
8	1233.0
9	1185.0
10	1137.0
11	1153.0
12	1169.0
13	1130.5
14	1092.0
15	1088.5
16	1085.0
17	1132.0
18	1179.0
19	1101.0
20	1023.0
21	1294.5
22	1566.0
23	1815.5
24	2065.0
25	2901.5
26	3995.0
27	4252.0
28	4900.5
29	5549.0
30	6512.5
31	7476.0
32	8745.5
33	10015.0
34	11488.0
35	12961.0
36	14034.0
37	15107.0
38	16114.5
39	17122.0
40	19391.5
41	21661.0
42	25034.0
43	28407.0
44	57420.5
45	86434.0
46	84688.5
47	82943.0
48	74693.0
49	66443.0
50	58876.5
51	51310.0
52	44695.0
53	38080.0
54	33259.0
55	28438.0
56	25495.5
57	22553.0
58	19923.0
59	17293.0
60	15618.0
61	13943.0
62	12098.0
63	10253.0
64	9337.0
65	8421.0
66	6888.5
67	5356.0
68	4798.5
69	4241.0
70	3881.0
71	3521.0
72	2963.0
73	2405.0
74	2184.5
75	1611.0
76	1258.0
77	973.0
78	688.0
79	522.5
80	357.0
81	339.0
82	321.0
83	248.0
84	175.0
85	126.5
86	78.0
87	52.0
88	26.0
89	18.0
90	10.0
91	6.5
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11543992848202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91994552755371	25.024333298207424
2	8.8625791143424	5.692512543161655
3	3.1370239112924594	3.022407089319741
4	1.6267536750717422	2.0897563972081556
5	1.0400906309910245	1.6701484089884606
6	0.7777392052539587	1.4986462035815313
7	0.635954852821245	1.4296778911105126
8	0.4834387895717713	1.2420679524472225
9	0.4134401989000026	1.1950032484613424
>10	4.817459599576584	32.992248137807444
>50	0.21536856526253256	4.345287554790532
>100	0.05753719022944965	3.4379850035561073
>500	0.005806505435999506	1.3211152432304112
>1k	0.004750777174908686	4.336302682739727
>5k	5.278641305454096E-4	1.083609474493621
>10k+	0.0015835923916362286	9.61889887089613
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	20903	3.528152812918275	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGT	18562	3.133022652891404	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	17275	2.9157938976779985	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	6392	1.0788859388687564	Illumina Single End Adapter 1 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT	4569	0.7711873990443285	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT	3929	0.6631637756281827	No Hit
AACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG	3600	0.6076328817158202	Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTAT	3335	0.5629043501450723	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3287	0.5548025783888615	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGTC	3166	0.5343793620867464	No Hit
AATGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC	1557	0.2628012213420922	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.18634075039285153	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTTGAGAGGTCGTATGCCGT	1032	0.17418809275853514	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATG	921	0.15545274557229735	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	869	0.14667582616973548	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC	807	0.13621103765129638	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA	793	0.1338480208890682	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	772	0.1303034957457259	No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	683	0.11528146061441812	Illumina Single End Adapter 1 (95% over 23bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.10836119723932128	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGCGGTCGTATGCC	596	0.10059699930628578	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.063607347631835E-4	0.0	0.0	1.2993216454023289	0.0
2	5.063607347631835E-4	0.0	0.0	1.649048126212101	0.0
3	5.063607347631835E-4	0.0	0.0	2.7090299309830317	0.0
4	5.063607347631835E-4	0.0	0.0	7.378857413880698	0.0
5	5.063607347631835E-4	0.0	0.0	7.963704062532175	0.0
6	5.063607347631835E-4	0.0	0.0	10.153039092736593	0.0
7	5.063607347631835E-4	0.0	0.0	11.930702845578542	0.0
8	5.063607347631835E-4	0.0	0.0	12.967729630373542	0.0
9	5.063607347631835E-4	0.0	0.0	17.485142532107492	0.0
10	5.063607347631835E-4	0.0	0.0	19.216558671174404	0.0
11	5.063607347631835E-4	0.0	0.0	24.673270735894057	0.0
12	5.063607347631835E-4	0.0	0.0	25.668607153526885	0.0
13	6.751476463509113E-4	0.0	0.0	26.294131447871006	0.0
14	6.751476463509113E-4	0.0	0.0	27.347024202355254	0.0
15	6.751476463509113E-4	0.0	0.0	27.794309518062732	0.0
16	8.439345579386393E-4	0.0	0.0	28.41831473020256	0.0
17	0.001012721469526367	0.0	0.0	29.084179096416147	0.0
18	0.001012721469526367	0.0	0.0	30.84901504397743	0.0
19	0.001012721469526367	0.0	0.0	31.352843975066797	0.0
20	0.001012721469526367	0.0	0.0	31.809750144734778	0.0
21	0.001012721469526367	0.0	0.0	32.310034550680804	0.0
22	0.001012721469526367	0.0	0.0	32.75411291506811	0.0
23	0.001012721469526367	0.0	0.0	33.2123693800288	0.0
24	0.001012721469526367	0.0	0.0	33.51247250883178	0.0
25	0.001012721469526367	0.0	0.0	33.784725797222784	0.0
26	0.0011815083811140949	0.0	0.0	34.05495364267473	0.0
27	0.0011815083811140949	0.0	0.0	34.38240025115493	0.0
28	0.0013502952927018227	0.0	0.0	34.675751903494394	0.0
29	0.0013502952927018227	0.0	0.0	34.98699496846216	0.0
30	0.0013502952927018227	0.0	0.0	35.49774416292663	0.0
31	0.0013502952927018227	0.0	0.0	35.80139181687295	0.0
32	0.0013502952927018227	0.0	0.0	36.0868104843678	0.0
33	0.0013502952927018227	0.0	0.0	36.37054128274677	0.0
34	0.0013502952927018227	0.0	0.0	36.64397607951889	0.0
35	0.0013502952927018227	0.0	0.0	36.938509240239476	0.0
36	0.0013502952927018227	0.0	0.0	37.21160646318842	0.0
37	0.0013502952927018227	0.0	0.0	37.46833135571335	0.0
38	0.0013502952927018227	0.0	0.0	37.73366438072926	0.0
39	0.0015190822042895505	0.0	0.0	38.00524252147392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACG	25	3.8885082E-5	45.000004	1
CCTAACG	35	1.210592E-7	45.000004	1
TCGTAGC	25	3.8885082E-5	45.000004	8
CGCACCG	25	3.8885082E-5	45.000004	1
TTCGCGG	35	1.210592E-7	45.000004	2
CGATCGA	25	3.8885082E-5	45.000004	18
CGATCAA	35	1.210592E-7	45.000004	18
CAATACG	35	1.210592E-7	45.000004	1
ACGCATG	20	7.030516E-4	45.0	1
CCGATCA	40	6.8048394E-9	45.0	17
CCTACCG	45	3.8380676E-10	45.0	1
TCTAGCG	45	3.8380676E-10	45.0	1
GCACCGT	20	7.030516E-4	45.0	24
TAGCAAC	20	7.030516E-4	45.0	11
ATTCACG	20	7.030516E-4	45.0	1
CGTAGTG	20	7.030516E-4	45.0	28
CGTAGCA	20	7.030516E-4	45.0	9
TAACACG	20	7.030516E-4	45.0	1
GGTATGC	45	3.8380676E-10	45.0	8
ACTCGCG	20	7.030516E-4	45.0	1
>>END_MODULE