FastQCFastQC Report
Sat 18 Jun 2016
SRR3548702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383667
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCC137413.5814912411023103No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGT119133.1050363987520426No Hit
AATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT113452.956991349269028No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT61221.5956545650264422Illumina Paired End PCR Primer 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38431.0016498682451187No Hit
AATGACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTT29830.7774971524785816No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT28080.7318846812470189No Hit
AACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG23090.601823977563877TruSeq Adapter, Index 21 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTAT20480.5337962347556605No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCGGTTTC17340.4519544292315993No Hit
AAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT13150.34274514096860037TruSeq Adapter, Index 21 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC12260.3195479413136913No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTT9100.2371848504041265No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8770.2285836415433175No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC8000.20851415420142988TruSeq Adapter, Index 21 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.16993903567416535No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTTCCGGTTTCGTATGCCGT6500.1694177502886618No Hit
AATCTGTCTCTTATACCCATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT6010.1566462583438242TruSeq Adapter, Index 21 (95% over 21bp)
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATG5920.15430047410905812No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC5290.1378799844656955No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT5100.13292777330341154No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTA5060.1318852025324044No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.12980006099039013No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4460.11624664096729716No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC3970.10347514902245958TruSeq Adapter, Index 21 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.10008679401668635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAG207.0274057E-445.035
CGGGTGC207.0274057E-445.06
CGGGTAC253.8859303E-545.06
TCGTTGA253.8859303E-545.024
AATCACG207.0274057E-445.01
CCTAGCG207.0274057E-445.01
TAGACGG406.7939254E-945.02
GAGTACG207.0274057E-445.01
CGTTGAT253.8859303E-545.025
AACCACG453.8380676E-1045.01
ATAGCGG207.0274057E-445.02
TAAACGG453.8380676E-1045.02
TAAACCG406.7939254E-945.01
ATTGTCC207.0274057E-445.013
GTGATAG207.0274057E-445.01
CGACAAG207.0274057E-445.01
TCGATCA1050.045.017
GTTACTT207.0274057E-445.014
TACATCG207.0274057E-445.01
TAGCACG253.8859303E-545.01
Produced by FastQC (version 0.11.5)