FastQCFastQC Report
Sat 18 Jun 2016
SRR3548700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394188
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC40761.0340243741564938No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGT36110.9160603569870215No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT33920.8605031101910764No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT31140.7899783859478218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27230.6907871371020934No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT8890.225526905943357No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT8540.2166478938983429No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG7940.20142673039260456No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT7720.19584563710716715No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.15804641440125017No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT6050.15348006534952865No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.12735040133134443No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT4640.11771033111104345No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC4540.11517347052675372No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACATC4350.11035343541660324No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.10680183059859762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG406.7957444E-945.0000041
TGTCATA207.027639E-445.00000430
CTAGACG207.027639E-445.0000041
ACAGTTG650.045.0000041
GGTACGA207.027639E-445.0000049
ATTAGCG207.027639E-445.0000041
CGCATCG207.027639E-445.0000041
CGTTGCA207.027639E-445.00000431
TAAACGG406.7957444E-945.0000042
ATTAACG207.027639E-445.0000041
TACGGCA207.027639E-445.00000415
CGACAAG207.027639E-445.0000041
CGTTATT207.027639E-445.00000431
ACGATTG207.027639E-445.0000041
AGTGCGT207.027639E-445.0000041
TCTACGG406.7957444E-945.0000042
TCGTAGG207.027639E-445.0000042
ATCGGTT207.027639E-445.00000418
GAATTAG207.027639E-445.00000442
CGATCGG207.027639E-445.0000042