##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394188
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.087803281682852	31.0	31.0	33.0	28.0	34.0
2	31.38830456533431	31.0	31.0	34.0	30.0	34.0
3	31.52230661511766	31.0	31.0	34.0	30.0	34.0
4	35.46556719128944	37.0	35.0	37.0	33.0	37.0
5	34.831801069540425	35.0	35.0	37.0	32.0	37.0
6	34.94235491694319	35.0	35.0	37.0	32.0	37.0
7	34.9395263173917	36.0	35.0	37.0	32.0	37.0
8	35.34305711995292	37.0	35.0	37.0	33.0	37.0
9	36.74740225476169	39.0	37.0	39.0	32.0	39.0
10	36.21810405187372	38.0	35.0	39.0	32.0	39.0
11	36.358960698955826	38.0	35.0	39.0	32.0	39.0
12	36.09793803971709	37.0	35.0	39.0	32.0	39.0
13	35.84862552893543	37.0	35.0	39.0	31.0	39.0
14	36.84810800937624	39.0	35.0	40.0	31.0	41.0
15	36.92112139385268	38.0	35.0	40.0	32.0	41.0
16	36.76093386911829	38.0	35.0	40.0	31.0	41.0
17	36.68366616944199	38.0	35.0	40.0	31.0	41.0
18	36.59514749307437	38.0	35.0	40.0	31.0	41.0
19	36.67217419099516	38.0	35.0	40.0	31.0	41.0
20	36.597154149796545	38.0	35.0	40.0	31.0	41.0
21	36.7310268196901	38.0	35.0	40.0	31.0	41.0
22	36.67685723563376	38.0	35.0	40.0	31.0	41.0
23	36.6130348970542	38.0	35.0	40.0	31.0	41.0
24	36.20000101474423	38.0	35.0	40.0	30.0	41.0
25	36.348721929637634	38.0	35.0	40.0	30.0	41.0
26	36.293167219702276	38.0	35.0	40.0	30.0	41.0
27	36.18469359797863	38.0	35.0	40.0	30.0	41.0
28	36.099756968756026	38.0	35.0	40.0	30.0	41.0
29	35.594967375972885	38.0	34.0	40.0	28.0	41.0
30	35.70540452778877	38.0	34.0	40.0	29.0	41.0
31	35.596141942423415	38.0	34.0	40.0	29.0	41.0
32	35.55851268937664	38.0	34.0	40.0	29.0	41.0
33	35.40213552923986	38.0	34.0	40.0	27.0	41.0
34	35.35343795346383	38.0	34.0	40.0	27.0	41.0
35	35.22661014541285	38.0	34.0	40.0	27.0	41.0
36	35.27107877459486	38.0	34.0	40.0	27.0	41.0
37	35.228789308654754	38.0	34.0	40.0	27.0	41.0
38	35.089840380732035	37.0	34.0	40.0	27.0	41.0
39	35.088714014632615	37.0	34.0	40.0	27.0	41.0
40	34.883268897074494	37.0	33.0	40.0	26.0	41.0
41	35.013321054928106	37.0	34.0	40.0	27.0	41.0
42	34.928947608755216	37.0	34.0	40.0	26.0	41.0
43	34.94499325195085	37.0	34.0	40.0	27.0	41.0
44	34.92938902249688	37.0	34.0	40.0	27.0	41.0
45	34.723809451327796	37.0	33.0	40.0	26.0	41.0
46	34.71949932519509	37.0	33.0	40.0	26.0	41.0
47	34.628349924401554	37.0	33.0	40.0	26.0	41.0
48	34.68826042396014	37.0	33.0	40.0	26.0	41.0
49	34.55247749804662	36.0	33.0	40.0	26.0	41.0
50	34.45143941469553	36.0	33.0	40.0	26.0	41.0
51	33.16578130232275	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	7.0
12	9.0
13	23.0
14	37.0
15	59.0
16	91.0
17	144.0
18	320.0
19	561.0
20	934.0
21	1469.0
22	1969.0
23	2347.0
24	2733.0
25	3170.0
26	3662.0
27	4392.0
28	5790.0
29	7468.0
30	9903.0
31	13333.0
32	17911.0
33	25621.0
34	38843.0
35	41476.0
36	46104.0
37	58881.0
38	66946.0
39	39973.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.812404233512943	34.965549433265345	18.609648188174173	25.612398145047543
2	23.634407947476838	33.68468852425746	18.54825616203436	24.132647366231343
3	19.88492800389662	30.50853907272672	23.556272641480717	26.050260281895948
4	23.263772616112107	31.15011111449359	18.75272712512811	26.83338914426619
5	17.124570002130962	36.12565577846104	18.60254497853816	28.147229240869837
6	20.396866469806284	35.728383410961264	23.06894172323866	20.80580839599379
7	76.7012187078247	9.845302241570012	7.561620343592397	5.891858707012897
8	78.0487990501994	5.998406851553066	6.227739048372857	9.725055049874678
9	74.07404588673425	8.357433508884085	11.048281530640203	6.520239073741463
10	37.519406983469814	28.22384243051539	15.522035171035139	18.734715414979654
11	29.155631323125007	28.46078520908805	23.33962474758237	19.043958720204575
12	27.83595644717749	23.672460856241184	30.99003521162491	17.50154748495642
13	25.80570692157042	29.46132302353192	24.806944909535552	19.92602514536211
14	18.088323338102633	31.544085563233786	30.552426760834933	19.81516433782865
15	17.287182765583932	27.451622068657596	32.40763290612601	22.853562259632458
16	18.932336854495823	26.822480643753742	30.822856099120216	23.422326402630215
17	17.827534070037647	25.539336560219994	28.594477761880118	28.038651607862235
18	21.88295940008321	25.62939511096228	27.392259530985214	25.095385957969295
19	22.625244807046386	30.28783220189351	26.81563112017616	20.271291870883942
20	23.645316447989284	30.144499578881145	23.578597014622467	22.631586958507107
21	21.894882644829373	28.85323754147767	26.250925954113264	23.000953859579692
22	20.339787106659767	29.004688118359766	25.52462276883112	25.130902006149352
23	21.283499244015548	29.139649101443982	23.189696287050847	26.387155367489623
24	18.405430911138847	30.176210336184766	26.641856170152312	24.776502582524074
25	20.046779709174302	27.82098896973018	26.308005317259784	25.824226003835733
26	21.552913838067113	30.037444062224118	23.99996955767299	24.40967254203578
27	19.199214587963105	27.876546216526126	27.00082194282931	25.923417252681464
28	21.02321734806742	29.583092331577827	25.90109287953971	23.49259744081504
29	20.282454057454817	32.04181760987143	24.838909352897602	22.836818979776147
30	24.70572417222239	27.399362740621225	24.3949587506469	23.499954336509482
31	23.902300425177835	28.93517813835023	20.08939896699037	27.073122469481568
32	24.916029914660008	27.55360386414604	21.706393903416643	25.823972317777304
33	23.19908267121272	29.888784031984738	22.258414766558086	24.65371853024445
34	18.57743005875369	29.851238495337252	23.42942961226623	28.14190183364283
35	21.184815367286674	31.51364323622231	24.266339919023412	23.035201477467602
36	22.929414391102725	29.061006423330998	24.473094056643024	23.536485128923253
37	21.837042223507567	29.86874283336885	20.92656295980598	27.367651983317604
38	21.06989558281835	30.072960110404175	23.259459953118817	25.59768435365866
39	20.33116178067318	27.216962464610795	23.956081869564777	28.49579388515125
40	22.421534902127917	28.69189320831684	22.388048342415296	26.498523547139946
41	19.569849919327833	25.356936284209564	27.075659330065854	27.997554466396746
42	24.213319532811756	28.395587892071806	22.24547677757821	25.14561579753823
43	21.210183973129574	26.012968431306888	26.341491876972412	26.43535571859113
44	22.528844104843373	24.420073670431368	25.88003693669011	27.17104528803515
45	24.241986057414227	26.67711853227394	25.644616274468017	23.43627913584381
46	19.798421057972337	25.660091124032185	29.444833429734036	25.09665438826144
47	21.241387358316334	27.372725704486182	24.725511684779853	26.66037525241763
48	21.50293768455661	23.448963438765258	30.469471419728656	24.578627456949476
49	23.211005915958882	23.69605365967508	27.63300760043431	25.45993282393173
50	20.650298842176827	23.69605365967508	26.463261185018318	29.190386313129775
51	20.376571585131966	23.54942311790313	30.691954092970867	25.38205120399403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3149.0
1	2442.5
2	1736.0
3	1171.5
4	607.0
5	796.5
6	986.0
7	899.0
8	812.0
9	796.0
10	780.0
11	754.5
12	729.0
13	741.5
14	754.0
15	764.5
16	775.0
17	846.0
18	917.0
19	922.5
20	928.0
21	981.0
22	1034.0
23	1300.0
24	1566.0
25	1667.5
26	1933.5
27	2098.0
28	2656.5
29	3215.0
30	4133.5
31	5052.0
32	5825.0
33	6598.0
34	7305.0
35	8012.0
36	8851.5
37	9691.0
38	10395.5
39	11100.0
40	12749.5
41	14399.0
42	17077.5
43	19756.0
44	28118.5
45	36481.0
46	38373.0
47	40265.0
48	41080.0
49	41895.0
50	39240.5
51	36586.0
52	33349.5
53	30113.0
54	26448.0
55	22783.0
56	20168.5
57	17554.0
58	16304.5
59	15055.0
60	13757.5
61	12460.0
62	11940.0
63	11420.0
64	9850.5
65	8281.0
66	7518.0
67	6755.0
68	5927.0
69	5099.0
70	4468.0
71	3837.0
72	3505.5
73	3174.0
74	2730.0
75	1829.5
76	1373.0
77	1307.5
78	1242.0
79	883.0
80	524.0
81	414.0
82	304.0
83	219.0
84	134.0
85	82.0
86	30.0
87	39.0
88	48.0
89	28.0
90	8.0
91	6.0
92	4.0
93	7.0
94	10.0
95	5.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.771657418331074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41279328320493	28.4072723595955
2	8.138584851852086	5.822613383809432
3	2.8407157485504384	3.0485133174001255
4	1.4870128532415472	2.127716574512466
5	0.9171384189033803	1.6403780663100769
6	0.6900203855543967	1.480990370622996
7	0.5117481901525823	1.2814256659812389
8	0.4538331294342156	1.2987490584967893
9	0.4039046952916295	1.300350635066481
>10	4.7930842311328385	38.61423453057091
>50	0.301303168284905	6.706381867077124
>100	0.04131343678610991	2.6772019102347775
>500	0.00498610443970292	1.2839459813146517
>1k	0.0035615031712163715	4.310226279007471
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC	4076	1.0340243741564938	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGT	3611	0.9160603569870215	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	3392	0.8605031101910764	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	3114	0.7899783859478218	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2723	0.6907871371020934	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT	889	0.225526905943357	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT	854	0.2166478938983429	No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	794	0.20142673039260456	No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	772	0.19584563710716715	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	623	0.15804641440125017	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT	605	0.15348006534952865	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	502	0.12735040133134443	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT	464	0.11771033111104345	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	454	0.11517347052675372	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACATC	435	0.11035343541660324	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.10680183059859762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5368605842897296E-4	0.0	0.0	0.9665438826143871	0.0
2	2.5368605842897296E-4	0.0	0.0	1.3024242239743473	0.0
3	2.5368605842897296E-4	0.0	0.0	1.8519082265315028	0.0
4	2.5368605842897296E-4	0.0	0.0	4.254568885912306	0.0
5	5.073721168579459E-4	0.0	0.0	4.673150882320111	0.0
6	5.073721168579459E-4	0.0	0.0	6.348493612185049	0.0
7	5.073721168579459E-4	0.0	0.0	7.754168061939988	0.0
8	5.073721168579459E-4	0.0	0.0	8.982262270794646	0.0
9	5.073721168579459E-4	0.0	0.0	11.19465838635372	0.0
10	5.073721168579459E-4	0.0	0.0	13.181781282027865	0.0
11	5.073721168579459E-4	0.0	0.0	15.885820978822288	0.0
12	5.073721168579459E-4	0.0	0.0	16.77143900879783	0.0
13	5.073721168579459E-4	0.0	0.0	17.239489786599286	0.0
14	5.073721168579459E-4	0.0	0.0	17.82195297675221	0.0
15	5.073721168579459E-4	0.0	0.0	18.183455610013496	0.0
16	5.073721168579459E-4	0.0	0.0	18.828579256598374	0.0
17	5.073721168579459E-4	0.0	0.0	19.596740641521304	0.0
18	5.073721168579459E-4	0.0	0.0	20.962586380102895	0.0
19	5.073721168579459E-4	0.0	0.0	21.390047388555715	0.0
20	5.073721168579459E-4	0.0	0.0	21.930906065126283	0.0
21	5.073721168579459E-4	0.0	0.0	22.38880940059058	0.0
22	5.073721168579459E-4	0.0	0.0	22.841892700944726	0.0
23	5.073721168579459E-4	0.0	0.0	23.297512861883163	0.0
24	5.073721168579459E-4	0.0	0.0	23.619440470029527	0.0
25	5.073721168579459E-4	0.0	0.0	23.904583599703695	0.0
26	5.073721168579459E-4	0.0	0.0	24.156747541782092	0.0
27	5.073721168579459E-4	0.0	0.0	24.496433174018488	0.0
28	5.073721168579459E-4	0.0	0.0	24.79451429267253	0.0
29	5.073721168579459E-4	0.0	0.0	25.095385957969295	0.0
30	5.073721168579459E-4	0.0	0.0	25.627619308553278	0.0
31	5.073721168579459E-4	0.0	0.0	25.91377718246116	0.0
32	5.073721168579459E-4	0.0	0.0	26.23240687184795	0.0
33	7.610581752869189E-4	0.0	0.0	26.53606908378743	0.0
34	0.0010147442337158918	0.0	0.0	26.81487006200087	0.0
35	0.0010147442337158918	0.0	0.0	27.11903964605721	0.0
36	0.0010147442337158918	0.0	0.0	27.37881416988848	0.0
37	0.0010147442337158918	0.0	0.0	27.649750880290622	0.0
38	0.0010147442337158918	0.0	0.0	27.93717718449065	0.0
39	0.0010147442337158918	0.0	0.0	28.244391001248136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	40	6.7957444E-9	45.000004	1
TGTCATA	20	7.027639E-4	45.000004	30
CTAGACG	20	7.027639E-4	45.000004	1
ACAGTTG	65	0.0	45.000004	1
GGTACGA	20	7.027639E-4	45.000004	9
ATTAGCG	20	7.027639E-4	45.000004	1
CGCATCG	20	7.027639E-4	45.000004	1
CGTTGCA	20	7.027639E-4	45.000004	31
TAAACGG	40	6.7957444E-9	45.000004	2
ATTAACG	20	7.027639E-4	45.000004	1
TACGGCA	20	7.027639E-4	45.000004	15
CGACAAG	20	7.027639E-4	45.000004	1
CGTTATT	20	7.027639E-4	45.000004	31
ACGATTG	20	7.027639E-4	45.000004	1
AGTGCGT	20	7.027639E-4	45.000004	1
TCTACGG	40	6.7957444E-9	45.000004	2
TCGTAGG	20	7.027639E-4	45.000004	2
ATCGGTT	20	7.027639E-4	45.000004	18
GAATTAG	20	7.027639E-4	45.000004	42
CGATCGG	20	7.027639E-4	45.000004	2
>>END_MODULE