FastQCFastQC Report
Sat 18 Jun 2016
SRR3548699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401356
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC42031.0471999920270285No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT37530.9350800785337706No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGT36630.9126560958351189No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT31670.789075035629217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27510.685426404488783No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT8770.21850925363019363No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT8350.2080447283708229No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT7920.19733104774813381No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG7780.19384287266167693No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.14949321799101045No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT5640.14052362491154985No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACATC5080.1265709245657222No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT5030.12532514774913045No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.12308274947926529No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC4850.12084035120940013No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.1083825830434826No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4130.10290116505047887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA850.045.00000425
ATCATAT302.161958E-645.00000442
TCACGCG207.0277945E-445.01
CGGGTCA207.0277945E-445.06
CGACGAT207.0277945E-445.034
CGACACG253.886254E-545.01
CGACAAG207.0277945E-445.01
AACGGTT207.0277945E-445.04
CTAAACG207.0277945E-445.01
CGGATAT253.886254E-545.023
ATTGGGC351.2092823E-745.04
TACAGCG207.0277945E-445.01
TATACGG207.0277945E-445.02
TCGACAG253.886254E-545.01
TATCGTG253.886254E-545.037
GCCGTAA207.0277945E-445.013
GCGTAAG253.886254E-545.01
GACTACG207.0277945E-445.01
CCAACGG950.042.631582
ACGGGAT2000.041.6255