##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401356
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1751636950737	31.0	31.0	33.0	28.0	34.0
2	31.462992953886324	31.0	31.0	34.0	30.0	34.0
3	31.615667885866912	31.0	31.0	34.0	30.0	34.0
4	35.52079450662255	37.0	35.0	37.0	33.0	37.0
5	34.858786214732056	35.0	35.0	37.0	32.0	37.0
6	34.97129730214573	35.0	35.0	37.0	32.0	37.0
7	34.97207964998655	36.0	35.0	37.0	32.0	37.0
8	35.3403786164901	37.0	35.0	37.0	33.0	37.0
9	36.83160585614766	39.0	37.0	39.0	33.0	39.0
10	36.26702976908281	38.0	35.0	39.0	32.0	39.0
11	36.367259988638516	38.0	35.0	39.0	32.0	39.0
12	36.08434158203689	37.0	35.0	39.0	32.0	39.0
13	35.930428347900616	37.0	35.0	39.0	31.0	39.0
14	36.89493865794955	39.0	35.0	40.0	31.0	41.0
15	36.948155752000716	38.0	35.0	40.0	32.0	41.0
16	36.78454788267772	38.0	35.0	40.0	31.0	41.0
17	36.73242707222516	38.0	35.0	40.0	31.0	41.0
18	36.61134753186697	38.0	35.0	40.0	31.0	41.0
19	36.71330688964411	38.0	35.0	40.0	31.0	41.0
20	36.627415561247375	38.0	35.0	40.0	31.0	41.0
21	36.74293395389629	38.0	35.0	40.0	31.0	41.0
22	36.71358096054376	38.0	35.0	40.0	31.0	41.0
23	36.61487308025792	38.0	35.0	40.0	31.0	41.0
24	36.21580591793819	38.0	35.0	40.0	30.0	41.0
25	36.331117511635554	38.0	35.0	40.0	30.0	41.0
26	36.224690798194125	38.0	35.0	40.0	30.0	41.0
27	36.08299863462861	38.0	35.0	40.0	30.0	41.0
28	35.96122395080676	38.0	34.0	40.0	30.0	41.0
29	35.56959905918935	38.0	34.0	40.0	28.0	41.0
30	35.67378835746818	38.0	34.0	40.0	29.0	41.0
31	35.56707760691257	38.0	34.0	40.0	28.0	41.0
32	35.50604949222137	38.0	34.0	40.0	28.0	41.0
33	35.33324529843829	38.0	34.0	40.0	27.0	41.0
34	35.28134623625908	38.0	34.0	40.0	27.0	41.0
35	35.12813811180099	38.0	34.0	40.0	26.0	41.0
36	35.16559862067591	38.0	34.0	40.0	27.0	41.0
37	35.12206121248966	37.0	34.0	40.0	27.0	41.0
38	35.05780902739713	37.0	34.0	40.0	27.0	41.0
39	35.073142048455736	37.0	34.0	40.0	27.0	41.0
40	34.803209619390266	37.0	33.0	40.0	26.0	41.0
41	34.9290156369906	37.0	34.0	40.0	26.0	41.0
42	34.88127746937881	37.0	33.0	40.0	26.0	41.0
43	34.91019693239917	37.0	33.0	40.0	26.0	41.0
44	34.84173153011292	37.0	33.0	40.0	26.0	41.0
45	34.665224887630934	37.0	33.0	40.0	26.0	41.0
46	34.62724364404668	37.0	33.0	40.0	26.0	41.0
47	34.51753057136308	37.0	33.0	40.0	26.0	41.0
48	34.588622071178705	37.0	33.0	40.0	26.0	41.0
49	34.42241301986267	36.0	33.0	40.0	26.0	41.0
50	34.35071108940691	36.0	33.0	40.0	26.0	41.0
51	32.95446685735357	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	10.0
12	16.0
13	15.0
14	27.0
15	52.0
16	79.0
17	179.0
18	327.0
19	599.0
20	978.0
21	1449.0
22	1972.0
23	2349.0
24	2838.0
25	3237.0
26	3821.0
27	4745.0
28	5937.0
29	7785.0
30	10252.0
31	13868.0
32	18496.0
33	25829.0
34	39347.0
35	42399.0
36	46908.0
37	59449.0
38	67645.0
39	40736.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.643020161652	35.08157346595043	18.608915775520984	25.66649059687659
2	23.375507031164354	33.9827983137165	18.568054295936776	24.07364035918237
3	19.809844626715435	30.44778201895574	23.822740908320792	25.919632446008034
4	23.29154167372607	31.208453343166664	18.634080467216137	26.865924515891127
5	16.962247979350003	36.16689422856517	18.72751372846052	28.143344063624316
6	20.491284545391125	35.686522688087386	23.053847457120362	20.76834530940113
7	76.55124129202005	9.915635993980407	7.653305295049781	5.87981741894976
8	77.88546826258982	5.969762505107685	6.217173780882807	9.927595451419688
9	73.99415979828382	8.32303491164951	11.207506552786056	6.475298737280618
10	37.58732895484308	28.16975453213606	15.505187414664286	18.73772909835657
11	29.037064351847235	28.478707182650815	23.4168668214752	19.067361644026747
12	27.6706963394094	23.7278127148965	31.161362979499497	17.4401279661946
13	25.77238162628689	29.400582026928713	24.866203569898047	19.960832776886356
14	18.19706195995575	31.496975253889314	30.61396864628908	19.691994139865855
15	17.301846739552914	27.574771524531837	32.33637967290884	22.78700206300641
16	19.04618343814469	26.65015597125744	30.70939515044001	23.594265440157866
17	17.82631877933804	25.73002521452277	28.638914081264517	27.804741924874676
18	21.696199882398666	25.56383858718943	27.63955191899461	25.100409611417295
19	22.479544344671563	30.473943332104163	26.885109478866642	20.161402844357628
20	23.587039934621632	30.19588594664089	23.700156469568164	22.516917649169315
21	21.73182909935319	29.099602348040143	26.145865515901097	23.02270303670557
22	20.110325994877364	28.959577033855233	25.718314912446804	25.211782058820596
23	21.319227817698003	28.974028044927696	23.35482713600893	26.351917001365372
24	18.350292508396535	30.375776118956736	26.454319855689214	24.81961151695751
25	19.945135988997297	27.92035001345439	26.242039486141977	25.892474511406334
26	21.465731171329196	29.99581418989625	24.156858250530703	24.381596388243853
27	19.237036446446545	27.729247849789214	27.049053707930113	25.984661995834124
28	21.003547972373653	29.593926588863752	25.91813751382812	23.48438792493447
29	20.219705199374122	32.1186178853686	24.738137713152415	22.923539202104866
30	24.712225555367304	27.427271549447376	24.42171040173811	23.438792493447213
31	23.87282113634778	29.017879388871727	20.113066703873866	26.996232770906627
32	25.004983107266366	27.50949281934243	21.59952760143115	25.885996471960055
33	23.180169226322768	29.939006767059666	22.151655886544614	24.729168120072952
34	18.554101595590947	29.664188401319525	23.59326881870459	28.18844118438493
35	21.215330031194252	31.504699070152185	24.21939624672361	23.060574651929958
36	22.987821285840997	29.0066673975224	24.446875093433263	23.55863622320334
37	21.935887341910924	29.911848832457967	20.763113046771444	27.389150778859666
38	21.002302195557064	30.19115199473784	23.24943441732527	25.557111392379834
39	20.385144360617506	27.144480212081046	23.98020709793799	28.490168329363456
40	22.232631379623076	28.764239229013643	22.569987741556126	26.43314164980715
41	19.49740380111422	25.43103877854075	26.912267413468342	28.159290006876684
42	24.191241690668633	28.285860931442407	22.276482723567106	25.246414654321846
43	21.159270074447623	25.977934801024528	26.431397562263925	26.431397562263925
44	22.55254686612384	24.34746210346924	25.951275177149462	27.148715853257453
45	24.32279572250072	26.564695681639243	25.561097878192925	23.55141071766711
46	19.880853905261166	25.59249145397104	29.543348050110126	24.98330659065767
47	21.22305384745712	27.235671075055564	24.931233119724137	26.61004195776318
48	21.360836763372166	23.445768843620126	30.515801433141647	24.677592959866054
49	23.21255942355415	23.640857493098398	27.74045984113854	25.40612324220891
50	20.847576714936366	23.506562752269804	26.553234534926602	29.092625997867227
51	20.352754163386123	23.458724922512683	30.882558127946265	25.305962786154936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3168.0
1	2439.0
2	1710.0
3	1172.5
4	635.0
5	813.0
6	991.0
7	876.5
8	762.0
9	737.0
10	712.0
11	760.0
12	808.0
13	801.0
14	794.0
15	821.5
16	849.0
17	882.5
18	916.0
19	974.0
20	1032.0
21	1061.5
22	1091.0
23	1355.0
24	1619.0
25	1716.0
26	1964.0
27	2115.0
28	2750.0
29	3385.0
30	4290.0
31	5195.0
32	5988.5
33	6782.0
34	7422.0
35	8062.0
36	8970.5
37	9879.0
38	10654.5
39	11430.0
40	12890.5
41	14351.0
42	17444.5
43	20538.0
44	28929.5
45	37321.0
46	39324.0
47	41327.0
48	41933.5
49	42540.0
50	39812.0
51	37084.0
52	33980.0
53	30876.0
54	26898.0
55	22920.0
56	20297.0
57	17674.0
58	16505.0
59	15336.0
60	14146.5
61	12957.0
62	12163.5
63	11370.0
64	9938.0
65	8506.0
66	7621.0
67	6736.0
68	5898.5
69	5061.0
70	4507.5
71	3954.0
72	3535.0
73	3116.0
74	2676.5
75	1789.5
76	1342.0
77	1304.0
78	1266.0
79	908.5
80	551.0
81	415.5
82	280.0
83	209.0
84	138.0
85	86.0
86	34.0
87	42.0
88	50.0
89	31.5
90	13.0
91	13.5
92	14.0
93	10.5
94	7.0
95	6.5
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.57076850914348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42967160635364	28.253744614668918
2	8.241738741309307	5.863299617599059
3	2.7865082899620126	2.97354723993144
4	1.388677378385836	1.9758528624178726
5	0.9568361211519242	1.7017698083340933
6	0.6941043848654198	1.4813895837137572
7	0.5532532058769193	1.3775749199232576
8	0.4517990786697757	1.2856672352005516
9	0.3786569999409441	1.212220844033947
>10	4.741539783836688	38.26405258153906
>50	0.32242348981046914	7.086950543164565
>100	0.04565909986422766	2.7785103846557124
>500	0.0056195815217510965	1.3635189900239408
>1k	0.0035122384510944352	4.381900774793816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC	4203	1.0471999920270285	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	3753	0.9350800785337706	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGT	3663	0.9126560958351189	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	3167	0.789075035629217	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2751	0.685426404488783	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT	877	0.21850925363019363	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT	835	0.2080447283708229	No Hit
AAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	792	0.19733104774813381	No Hit
AACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	778	0.19384287266167693	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	600	0.14949321799101045	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT	564	0.14052362491154985	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACATC	508	0.1265709245657222	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTT	503	0.12532514774913045	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	494	0.12308274947926529	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	485	0.12084035120940013	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.1083825830434826	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	413	0.10290116505047887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.474660899550523E-4	0.0	0.0	0.9926349674603095	0.0
2	7.474660899550523E-4	0.0	0.0	1.350172913822143	0.0
3	7.474660899550523E-4	0.0	0.0	1.8940790719461027	0.0
4	7.474660899550523E-4	0.0	0.0	4.357727304437955	0.0
5	7.474660899550523E-4	0.0	0.0	4.776557470176103	0.0
6	7.474660899550523E-4	0.0	0.0	6.452625599218649	0.0
7	7.474660899550523E-4	0.0	0.0	7.84839394452805	0.0
8	7.474660899550523E-4	0.0	0.0	9.10513359710581	0.0
9	7.474660899550523E-4	0.0	0.0	11.32934352544873	0.0
10	7.474660899550523E-4	0.0	0.0	13.331805180438314	0.0
11	9.966214532734032E-4	0.0	0.0	16.056817389051115	0.0
12	9.966214532734032E-4	0.0	0.0	16.992146622948205	0.0
13	9.966214532734032E-4	0.0	0.0	17.481736911868765	0.0
14	9.966214532734032E-4	0.0	0.0	18.032619420165638	0.0
15	9.966214532734032E-4	0.0	0.0	18.412083038499485	0.0
16	9.966214532734032E-4	0.0	0.0	19.082560121189168	0.0
17	9.966214532734032E-4	0.0	0.0	19.8242956377879	0.0
18	9.966214532734032E-4	0.0	0.0	21.194899291402148	0.0
19	9.966214532734032E-4	0.0	0.0	21.61871256440666	0.0
20	9.966214532734032E-4	0.0	0.0	22.14941348827475	0.0
21	9.966214532734032E-4	0.0	0.0	22.60262709415083	0.0
22	0.001245776816591754	0.0	0.0	23.060823807293275	0.0
23	0.001245776816591754	0.0	0.0	23.524501938428727	0.0
24	0.001245776816591754	0.0	0.0	23.856626037732088	0.0
25	0.001245776816591754	0.0	0.0	24.15112767717438	0.0
26	0.001245776816591754	0.0	0.0	24.401030506582686	0.0
27	0.001245776816591754	0.0	0.0	24.742373354328826	0.0
28	0.001245776816591754	0.0	0.0	25.033386818684658	0.0
29	0.001245776816591754	0.0	0.0	25.335113963663183	0.0
30	0.001245776816591754	0.0	0.0	25.847128235282394	0.0
31	0.001245776816591754	0.0	0.0	26.150101157077508	0.0
32	0.001245776816591754	0.0	0.0	26.46104705049881	0.0
33	0.001245776816591754	0.0	0.0	26.7378586591455	0.0
34	0.001245776816591754	0.0	0.0	27.005700674712724	0.0
35	0.001245776816591754	0.0	0.0	27.32063305394712	0.0
36	0.001245776816591754	0.0	0.0	27.580751253251478	0.0
37	0.001245776816591754	0.0	0.0	27.848593268818703	0.0
38	0.001245776816591754	0.0	0.0	28.11668443974925	0.0
39	0.001245776816591754	0.0	0.0	28.39598760202912	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGA	85	0.0	45.000004	25
ATCATAT	30	2.161958E-6	45.000004	42
TCACGCG	20	7.0277945E-4	45.0	1
CGGGTCA	20	7.0277945E-4	45.0	6
CGACGAT	20	7.0277945E-4	45.0	34
CGACACG	25	3.886254E-5	45.0	1
CGACAAG	20	7.0277945E-4	45.0	1
AACGGTT	20	7.0277945E-4	45.0	4
CTAAACG	20	7.0277945E-4	45.0	1
CGGATAT	25	3.886254E-5	45.0	23
ATTGGGC	35	1.2092823E-7	45.0	4
TACAGCG	20	7.0277945E-4	45.0	1
TATACGG	20	7.0277945E-4	45.0	2
TCGACAG	25	3.886254E-5	45.0	1
TATCGTG	25	3.886254E-5	45.0	37
GCCGTAA	20	7.0277945E-4	45.0	13
GCGTAAG	25	3.886254E-5	45.0	1
GACTACG	20	7.0277945E-4	45.0	1
CCAACGG	95	0.0	42.63158	2
ACGGGAT	200	0.0	41.625	5
>>END_MODULE