SRR3548698_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406929
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	8938	2.1964519609071855	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	8173	2.0084584780145924	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGT	7744	1.9030346817258046	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	3697	0.9085122957567536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2165	0.532033843741783	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	2081	0.5113914220908315	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	1883	0.4627342853421605	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTG	1644	0.4040016808829059	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTAT	1406	0.34551481953854357	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGTC	1290	0.31700861821103926	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	862	0.21183056503714406	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	742	0.18234139125007065	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	624	0.1533437036927818	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	501	0.12311730056103153	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.12262581433124697	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.11721946580361683	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	451	0.1108301448164176	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.10345785136964925	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATG	412	0.10124616333561874	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTAGCAGAGTCGTATGCCGT	408	0.10026319087604962	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTA	45	3.8380676E-10	45.000004	5
AAGCACG	45	3.8380676E-10	45.000004	1
TTCGCGG	30	2.1620326E-6	45.000004	2
AGGGCGT	30	2.1620326E-6	45.000004	6
CCGCATC	30	2.1620326E-6	45.000004	20
GCCAACG	30	2.1620326E-6	45.000004	1
CGCTTAG	30	2.1620326E-6	45.000004	1
CTTACCG	30	2.1620326E-6	45.000004	1
CGGGTCA	35	1.2093369E-7	45.0	6
CGAACAG	20	7.02791E-4	45.0	1
CTGTACG	20	7.02791E-4	45.0	1
GGTAAAG	20	7.02791E-4	45.0	9
CGCGGGT	35	1.2093369E-7	45.0	4
CCGATCA	20	7.02791E-4	45.0	17
GGCCGAT	40	6.7957444E-9	45.0	8
TTCGTCG	20	7.02791E-4	45.0	37
TCGATTA	20	7.02791E-4	45.0	15
CGCTACG	40	6.7957444E-9	45.0	1
GCGGGTT	40	6.7957444E-9	45.0	5
GGAACGT	20	7.02791E-4	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content