##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406929
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14823224690302	31.0	31.0	33.0	28.0	34.0
2	31.426880364879377	31.0	31.0	34.0	30.0	34.0
3	31.538777034814427	31.0	31.0	34.0	30.0	34.0
4	35.44523246069953	37.0	35.0	37.0	33.0	37.0
5	34.755394675729676	35.0	35.0	37.0	32.0	37.0
6	34.88497747764352	35.0	35.0	37.0	32.0	37.0
7	34.79201039984862	35.0	35.0	37.0	32.0	37.0
8	35.23117792047261	37.0	35.0	37.0	33.0	37.0
9	36.715070688006996	39.0	35.0	39.0	32.0	39.0
10	36.15699298894893	37.0	35.0	39.0	32.0	39.0
11	36.40553266048868	38.0	35.0	39.0	32.0	39.0
12	36.11675255388532	37.0	35.0	39.0	32.0	39.0
13	35.911517734051884	37.0	35.0	39.0	31.0	39.0
14	36.89398887766662	39.0	35.0	40.0	31.0	41.0
15	36.95856279596686	38.0	36.0	40.0	32.0	41.0
16	36.73141014771619	38.0	35.0	40.0	31.0	41.0
17	36.69477476414804	38.0	35.0	40.0	31.0	41.0
18	36.53889007664728	38.0	35.0	40.0	31.0	41.0
19	36.540966605968116	38.0	35.0	40.0	30.0	41.0
20	36.62944641448508	38.0	35.0	40.0	31.0	41.0
21	36.86187270998135	39.0	35.0	40.0	31.0	41.0
22	36.836669296118	39.0	35.0	40.0	31.0	41.0
23	36.77907448228069	39.0	35.0	40.0	31.0	41.0
24	36.33770264591612	38.0	35.0	40.0	30.0	41.0
25	36.57971292289318	38.0	35.0	40.0	31.0	41.0
26	36.44934128558053	38.0	35.0	40.0	31.0	41.0
27	36.413448046219365	38.0	35.0	40.0	30.0	41.0
28	36.42106362534987	38.0	35.0	40.0	30.0	41.0
29	35.87153532925891	38.0	34.0	40.0	29.0	41.0
30	36.12181731948325	38.0	35.0	40.0	30.0	41.0
31	35.9169732312025	38.0	35.0	40.0	29.0	41.0
32	35.83228769637947	38.0	34.0	40.0	29.0	41.0
33	35.88773717282376	38.0	35.0	40.0	30.0	41.0
34	35.61961914732054	38.0	34.0	40.0	28.0	41.0
35	35.76216489854495	38.0	35.0	40.0	29.0	41.0
36	35.53761466988099	38.0	34.0	40.0	28.0	41.0
37	35.52209353474439	38.0	34.0	40.0	28.0	41.0
38	35.44831407936028	38.0	34.0	40.0	28.0	41.0
39	35.1911635690747	38.0	34.0	40.0	27.0	41.0
40	35.03898223031536	38.0	34.0	40.0	26.0	41.0
41	35.0723172838505	38.0	34.0	40.0	26.0	41.0
42	35.172845877290634	38.0	34.0	40.0	27.0	41.0
43	35.176652438140316	38.0	34.0	40.0	27.0	41.0
44	35.23562587085216	38.0	34.0	40.0	28.0	41.0
45	35.01532945550698	37.0	34.0	40.0	27.0	41.0
46	34.914980254540716	37.0	34.0	40.0	26.0	41.0
47	34.85895082434528	37.0	33.0	40.0	26.0	41.0
48	34.90667413725736	37.0	34.0	40.0	26.0	41.0
49	34.8331330526947	37.0	34.0	40.0	26.0	41.0
50	34.673301730768756	37.0	34.0	40.0	26.0	41.0
51	33.418303930169635	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	0.0
10	4.0
11	10.0
12	7.0
13	11.0
14	25.0
15	29.0
16	58.0
17	123.0
18	265.0
19	466.0
20	896.0
21	1448.0
22	1870.0
23	2348.0
24	2740.0
25	2986.0
26	3500.0
27	4476.0
28	5607.0
29	7446.0
30	9937.0
31	13605.0
32	18471.0
33	25204.0
34	37244.0
35	41248.0
36	46243.0
37	61380.0
38	73569.0
39	45710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.20447547360842	39.735678705621865	18.376670131644588	24.683175689125132
2	20.741947612482768	38.79767723607804	17.755185794082013	22.70518935735718
3	18.257976207151614	28.154788673208348	28.52487780423612	25.062357315403915
4	25.759776275468205	28.683382113341636	18.437122937908086	27.119718673282073
5	15.908426285666541	39.051038387531975	18.79983977548909	26.240695551312392
6	19.569261468216816	33.01976511873079	26.623809067429455	20.787164345622948
7	72.9289384634666	12.808868377530233	8.315701264839813	5.946491894163356
8	74.6442254054147	4.950249306390058	6.245807008102151	14.159718280093086
9	73.00659328777256	8.400974125707434	11.556315720924289	7.036116865595718
10	33.583008338063884	33.09447102565804	14.90849755116986	18.414023085108212
11	24.855195869549725	24.754195449328996	29.066249886343808	21.324358794777467
12	27.314101477161863	21.512843763899845	34.461785716918676	16.711269042019616
13	24.49886835295592	30.631633528207626	24.466676004905032	20.402822113931425
14	16.049482833614707	29.889489321232944	33.98332387222341	20.07770397292894
15	13.607287757815245	30.777359195338743	29.793649506424952	25.821703540421055
16	16.255169820779546	27.50135773070978	32.75215086661309	23.49132158189758
17	14.838215020310669	27.468428153314218	29.19256184739844	28.50079497897668
18	18.126257897569356	27.098830508516226	28.003410914434706	26.771500679479715
19	19.04804032153029	31.4917344303306	29.504901346426525	19.955323901712585
20	20.95992175539222	31.801125011979973	26.40804661255403	20.830906620073772
21	17.659345979274025	28.876781944761866	29.004568364505843	24.459303711458265
22	17.570632714797913	29.91848700879023	27.13495474640539	25.375925530006466
23	19.6110377977485	29.270708157934184	23.235011513064933	27.883242531252378
24	15.248851765295665	34.78813257349562	27.08752632523118	22.875489335977527
25	17.689818125520667	26.59309117806792	28.429529475657915	27.2875612207535
26	21.14226314664229	31.728876536201646	23.13990892760162	23.988951389554444
27	17.069562503532556	28.45926439255988	27.851541669431278	26.619631434476283
28	18.852428802076037	27.446802759203692	30.425209311698108	23.27555912702216
29	18.133630191016124	33.75183385799488	26.70318409353966	21.411351857449333
30	24.938994271727992	25.524108628286506	26.00134175740731	23.535555342578192
31	23.042594654104278	31.618783620729907	21.639155724954477	23.699466000211338
32	23.837327887665907	29.184943810836778	22.363360684542023	24.614367616955292
33	25.7725549174426	26.014611885611494	25.687036313460087	22.525796883485818
34	17.352658571888462	32.558259548963086	24.494199233772967	25.594882645375485
35	25.124776066586556	25.186211845309625	27.94787297046905	21.741139117634773
36	22.436100646550138	29.872533046305378	26.52084270228959	21.170523604854903
37	24.136643001604703	30.27014540620108	22.024480929105568	23.568730663088648
38	24.561041361023666	27.348014027016998	25.382560594108554	22.70838401785078
39	20.25291881384713	30.17700876565691	26.125442030427916	23.444630390068046
40	27.506272593007626	26.951384639580862	23.058567956572276	22.48377481083924
41	20.07696674358426	24.92252948303021	31.381395771743964	23.619108001641564
42	26.331620503822535	25.793688825323336	23.325444979345292	24.54924569150884
43	21.77972078667286	25.108557021003662	27.624720774385704	25.487001417937773
44	21.737207227796496	23.6306579280415	26.26084648673356	28.371288357428448
45	25.733481762174726	27.072781738337646	25.53762449960558	21.656111999882043
46	19.210968006703872	25.203905349581866	34.01158433043602	21.57354231327824
47	22.71698502687201	27.340150247340446	25.313998265053613	24.628866460733935
48	20.627431320942964	23.27039852160942	33.2070213722787	22.89514878516891
49	23.5033629945273	22.030133020748092	29.653575930936356	24.81292805378825
50	21.08525074398728	22.311017401069964	27.584173160428477	29.019558694514274
51	18.86791061831425	22.600502790413067	33.45694212012415	25.074644471148527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2512.0
1	1978.5
2	1445.0
3	1014.0
4	583.0
5	778.5
6	974.0
7	925.0
8	876.0
9	834.5
10	793.0
11	771.5
12	750.0
13	798.0
14	846.0
15	837.5
16	829.0
17	816.5
18	804.0
19	818.0
20	832.0
21	954.5
22	1077.0
23	1278.0
24	1479.0
25	1917.5
26	2758.0
27	3160.0
28	3693.5
29	4227.0
30	4877.5
31	5528.0
32	6591.0
33	7654.0
34	8747.5
35	9841.0
36	11213.5
37	12586.0
38	12897.5
39	13209.0
40	14570.5
41	15932.0
42	18542.0
43	21152.0
44	35824.0
45	50496.0
46	50325.5
47	50155.0
48	47648.5
49	45142.0
50	41808.0
51	38474.0
52	34583.5
53	30693.0
54	25835.5
55	20978.0
56	18677.0
57	16376.0
58	14569.0
59	12762.0
60	11222.5
61	9683.0
62	8314.5
63	6946.0
64	5987.0
65	5028.0
66	4231.0
67	3434.0
68	2975.5
69	2517.0
70	2109.5
71	1702.0
72	1469.0
73	1236.0
74	1069.5
75	716.0
76	529.0
77	365.0
78	201.0
79	155.5
80	110.0
81	86.0
82	62.0
83	43.5
84	25.0
85	20.0
86	15.0
87	12.0
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.129486996077134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32934303537459	26.73340294537082
2	8.424720082609026	5.750627490099893
3	2.9664693465013046	3.0373223095703312
4	1.5878676648333898	2.1677243527369017
5	1.042762824105549	1.7794480122651506
6	0.7621869987578931	1.5607830757611942
7	0.6608166267379607	1.5787332728331354
8	0.5412080445388926	1.4776922334609974
9	0.43262721642341323	1.3288810461364737
>10	4.9684197957837535	35.94634825401503
>50	0.21774767261378009	4.620193918986231
>100	0.055702892994222805	3.7108702293786697
>500	0.002893656778920665	0.6737834235511903
>1k	0.005063899363111164	3.497550743138938
>5k	0.0021702425841904987	6.136638692695065
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	8938	2.1964519609071855	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	8173	2.0084584780145924	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGT	7744	1.9030346817258046	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	3697	0.9085122957567536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2165	0.532033843741783	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	2081	0.5113914220908315	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	1883	0.4627342853421605	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTG	1644	0.4040016808829059	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTAT	1406	0.34551481953854357	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGTC	1290	0.31700861821103926	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	862	0.21183056503714406	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	742	0.18234139125007065	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	624	0.1533437036927818	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	501	0.12311730056103153	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.12262581433124697	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.11721946580361683	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	451	0.1108301448164176	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.10345785136964925	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATG	412	0.10124616333561874	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTAGCAGAGTCGTATGCCGT	408	0.10026319087604962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.91486229784557E-4	0.0	0.0	1.1584330436022008	0.0
2	4.91486229784557E-4	0.0	0.0	1.4874830744429617	0.0
3	4.91486229784557E-4	0.0	0.0	2.3158831147448327	0.0
4	4.91486229784557E-4	0.0	0.0	6.131044973447456	0.0
5	4.91486229784557E-4	0.0	0.0	6.685195697529545	0.0
6	4.91486229784557E-4	0.0	0.0	8.626812048293436	0.0
7	4.91486229784557E-4	0.0	0.0	10.190966974582789	0.0
8	4.91486229784557E-4	0.0	0.0	11.20047969056027	0.0
9	4.91486229784557E-4	0.0	0.0	14.433476110083086	0.0
10	4.91486229784557E-4	0.0	0.0	16.05390620968277	0.0
11	4.91486229784557E-4	0.0	0.0	20.027572377490912	0.0
12	4.91486229784557E-4	0.0	0.0	21.010299093945136	0.0
13	4.91486229784557E-4	0.0	0.0	21.55462009343153	0.0
14	4.91486229784557E-4	0.0	0.0	22.32133861189544	0.0
15	4.91486229784557E-4	0.0	0.0	22.71256165080395	0.0
16	4.91486229784557E-4	0.0	0.0	23.290057970800802	0.0
17	4.91486229784557E-4	0.0	0.0	23.98428227037149	0.0
18	4.91486229784557E-4	0.0	0.0	25.416964630193473	0.0
19	4.91486229784557E-4	0.0	0.0	25.887316952097294	0.0
20	4.91486229784557E-4	0.0	0.0	26.331866246937427	0.0
21	4.91486229784557E-4	0.0	0.0	26.8292503114794	0.0
22	4.91486229784557E-4	0.0	0.0	27.30500898191085	0.0
23	4.91486229784557E-4	0.0	0.0	27.800672844648574	0.0
24	4.91486229784557E-4	0.0	0.0	28.116698490400044	0.0
25	4.91486229784557E-4	0.0	0.0	28.400777531215518	0.0
26	4.91486229784557E-4	0.0	0.0	28.691491636133083	0.0
27	4.91486229784557E-4	0.0	0.0	29.024719299927014	0.0
28	4.91486229784557E-4	0.0	0.0	29.332389679772145	0.0
29	4.91486229784557E-4	0.0	0.0	29.665125857336292	0.0
30	4.91486229784557E-4	0.0	0.0	30.145307903835803	0.0
31	7.372293446768355E-4	0.0	0.0	30.443394302200137	0.0
32	7.372293446768355E-4	0.0	0.0	30.716660645960353	0.0
33	7.372293446768355E-4	0.0	0.0	31.02998311744801	0.0
34	7.372293446768355E-4	0.0	0.0	31.28678467251044	0.0
35	7.372293446768355E-4	0.0	0.0	31.62246976745329	0.0
36	7.372293446768355E-4	0.0	0.0	31.931614605987775	0.0
37	7.372293446768355E-4	0.0	0.0	32.18669595924596	0.0
38	7.372293446768355E-4	0.0	0.0	32.49633228401023	0.0
39	7.372293446768355E-4	0.0	0.0	32.77574220564275	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTA	45	3.8380676E-10	45.000004	5
AAGCACG	45	3.8380676E-10	45.000004	1
TTCGCGG	30	2.1620326E-6	45.000004	2
AGGGCGT	30	2.1620326E-6	45.000004	6
CCGCATC	30	2.1620326E-6	45.000004	20
GCCAACG	30	2.1620326E-6	45.000004	1
CGCTTAG	30	2.1620326E-6	45.000004	1
CTTACCG	30	2.1620326E-6	45.000004	1
CGGGTCA	35	1.2093369E-7	45.0	6
CGAACAG	20	7.02791E-4	45.0	1
CTGTACG	20	7.02791E-4	45.0	1
GGTAAAG	20	7.02791E-4	45.0	9
CGCGGGT	35	1.2093369E-7	45.0	4
CCGATCA	20	7.02791E-4	45.0	17
GGCCGAT	40	6.7957444E-9	45.0	8
TTCGTCG	20	7.02791E-4	45.0	37
TCGATTA	20	7.02791E-4	45.0	15
CGCTACG	40	6.7957444E-9	45.0	1
GCGGGTT	40	6.7957444E-9	45.0	5
GGAACGT	20	7.02791E-4	45.0	8
>>END_MODULE