FastQCFastQC Report
Sat 18 Jun 2016
SRR3548697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414391
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC95682.308930454570683No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT82731.9964236675024314No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGT81031.9553996105127764No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT38180.9213520563911861No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT22460.5420001882280262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21740.5246252935029959No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT19300.4657437058237269No Hit
AACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTG17030.4109645238434233No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTAT15140.3653554251902189No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGTC13810.3332601335453714No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC8720.21042928055869942No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT7080.17085313146279724No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6230.15034110296796988No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.12838116657939No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT5090.12283085298667201No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATG4930.11896976526999863No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC4760.11486735957103315No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTAGCAGAGTCGTATGCCGT4430.1069038661553943No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.10666254817310221No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.1035254144033051No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT207.028059E-445.026
CGGGTCA207.028059E-445.06
CGGGTAT800.045.06
CGGGTAG351.2094097E-745.06
CTCACGG502.1827873E-1145.02
CGAACAG253.8864724E-545.01
CGAGTAT351.2094097E-745.016
CGAGTAG351.2094097E-745.01
CGTGCGG253.8864724E-545.02
TCCGAGT351.2094097E-745.014
GCGAAAA207.028059E-445.029
TCTTACG207.028059E-445.01
GCCGGGT207.028059E-445.04
ACGGGTA700.045.05
TAGCCGT253.8864724E-545.044
ACGGGCC406.7975634E-945.05
TGCATCG207.028059E-445.01
ATCCGCG207.028059E-445.01
GTAGCCG253.8864724E-545.043
CTAACCG253.8864724E-545.01
Produced by FastQC (version 0.11.5)