##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414391
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207535877951017	31.0	31.0	33.0	30.0	34.0
2	31.492409342867003	31.0	31.0	34.0	30.0	34.0
3	31.615672155041977	31.0	31.0	34.0	30.0	34.0
4	35.48965349150923	37.0	35.0	37.0	33.0	37.0
5	34.77257710712829	35.0	35.0	37.0	32.0	37.0
6	34.9039288980697	35.0	35.0	37.0	32.0	37.0
7	34.816914460014814	35.0	35.0	37.0	32.0	37.0
8	35.22086387011301	37.0	35.0	37.0	33.0	37.0
9	36.78785012222756	39.0	37.0	39.0	33.0	39.0
10	36.20713046374077	37.0	35.0	39.0	32.0	39.0
11	36.387749733946926	38.0	35.0	39.0	32.0	39.0
12	36.089659283140804	37.0	35.0	39.0	32.0	39.0
13	35.9643404417567	37.0	35.0	39.0	31.0	39.0
14	36.93585526712694	39.0	36.0	40.0	31.0	41.0
15	36.981712923301906	38.0	36.0	40.0	32.0	41.0
16	36.75358779510173	38.0	35.0	40.0	31.0	41.0
17	36.72070339365479	38.0	35.0	40.0	31.0	41.0
18	36.520112647234136	38.0	35.0	40.0	31.0	41.0
19	36.57914385206242	38.0	35.0	40.0	31.0	41.0
20	36.64139182559467	38.0	35.0	40.0	31.0	41.0
21	36.87273613567862	39.0	35.0	40.0	31.0	41.0
22	36.86584409410436	39.0	35.0	40.0	31.0	41.0
23	36.78221776052086	39.0	35.0	40.0	31.0	41.0
24	36.351431377611966	38.0	35.0	40.0	30.0	41.0
25	36.56472268944065	38.0	35.0	40.0	31.0	41.0
26	36.372744581808	38.0	35.0	40.0	30.0	41.0
27	36.3194181340811	38.0	35.0	40.0	30.0	41.0
28	36.282938094698004	38.0	35.0	40.0	30.0	41.0
29	35.85356342198551	38.0	34.0	40.0	29.0	41.0
30	36.09539541157989	38.0	35.0	40.0	30.0	41.0
31	35.88041728705498	38.0	35.0	40.0	29.0	41.0
32	35.8002466269779	38.0	34.0	40.0	29.0	41.0
33	35.81309439635513	38.0	35.0	40.0	29.0	41.0
34	35.56676423957084	38.0	34.0	40.0	28.0	41.0
35	35.71130164506469	38.0	34.0	40.0	29.0	41.0
36	35.43151033685577	38.0	34.0	40.0	27.0	41.0
37	35.42815601690191	38.0	34.0	40.0	28.0	41.0
38	35.397325231484274	38.0	34.0	40.0	28.0	41.0
39	35.13712653025765	38.0	34.0	40.0	26.0	41.0
40	34.965725606975056	37.0	33.0	40.0	26.0	41.0
41	34.98662133106173	37.0	33.0	40.0	26.0	41.0
42	35.09584426302695	38.0	34.0	40.0	27.0	41.0
43	35.0853324517183	38.0	34.0	40.0	26.0	41.0
44	35.11583745785985	37.0	34.0	40.0	27.0	41.0
45	34.87260099760854	37.0	33.0	40.0	26.0	41.0
46	34.74102960730325	37.0	33.0	40.0	26.0	41.0
47	34.674628551295754	37.0	33.0	40.0	26.0	41.0
48	34.72827112557947	37.0	33.0	40.0	26.0	41.0
49	34.61700423030423	37.0	33.0	40.0	26.0	41.0
50	34.48683248429623	37.0	33.0	40.0	25.0	41.0
51	33.14849984676308	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	4.0
12	7.0
13	8.0
14	23.0
15	26.0
16	67.0
17	129.0
18	251.0
19	505.0
20	913.0
21	1397.0
22	1885.0
23	2252.0
24	2693.0
25	3264.0
26	3724.0
27	4556.0
28	6068.0
29	7972.0
30	10639.0
31	14264.0
32	18872.0
33	26033.0
34	38006.0
35	42353.0
36	47170.0
37	61411.0
38	73414.0
39	46475.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.943418172691974	40.081227632839514	18.358265502870477	24.61708869159803
2	20.53036866148155	39.34110538114969	17.736630380486062	22.391895576882703
3	18.042138946067844	28.101237719931177	29.0061801535265	24.85044318047448
4	26.02252462046714	28.64251395421233	18.462997507185243	26.87196391813529
5	15.778817590150366	39.38767975173206	18.833661927985887	25.999840730131684
6	19.500423513058923	32.888503852641584	26.832146451057092	20.778926183242397
7	72.49385242440111	13.068816649010234	8.36311599431455	6.074214932274109
8	74.25523237715105	4.911062257626251	6.368864188652745	14.464841176569953
9	72.7076601567119	8.321367983378018	11.694993375821387	7.275978484088699
10	33.44329389393109	33.19521900813483	15.027353393292808	18.33413370464127
11	24.929112842701702	24.625534820978253	29.088228267505812	21.357124068814237
12	27.09276987193255	21.547523956842692	34.65736466284258	16.70234150838218
13	24.612986285899066	30.360215352167398	24.342951463714222	20.683846898219315
14	15.910094572517261	29.94707896648335	34.21430484735431	19.928521613645085
15	13.590787444708017	30.749461257604533	29.659186613608885	26.000564684078565
16	16.256385877106403	27.470432514219663	32.97537832626674	23.297803282407195
17	14.794240222398653	27.80248605785357	29.205508806899765	28.19776491284801
18	18.072062375872065	27.104353135082565	28.087241276958235	26.73634321208713
19	18.857311090250512	31.567770535557	29.703830440332922	19.87108793385957
20	20.82574187180706	31.91068338839405	26.405496258364686	20.8580784814342
21	17.5373017271128	28.91110086850342	28.938611118484715	24.612986285899066
22	17.494105808282516	29.9576969577042	27.16757844644309	25.380618787570192
23	19.521659495500625	29.176550649024712	23.193312596074723	28.10847725939994
24	15.203515520366032	34.93536297844307	27.19388210651293	22.667239394677974
25	17.66133917001093	26.55969844904933	28.401678607884822	27.377283773054916
26	21.018072303693856	31.87376173710336	23.056002664150522	24.052163295052257
27	16.950175076196153	28.310701728560705	28.133574329558314	26.605548865684824
28	19.126621958488478	27.292339843288104	30.14327048608681	23.437767712136605
29	18.32858339104855	33.791757060360865	26.621234534533812	21.258425014056773
30	25.133267855720803	25.6151798663581	25.66682191456861	23.584730363352485
31	22.939204760721154	31.819706509069935	21.531838287993708	23.7092504422152
32	23.774164979451772	29.21226571040394	22.13971828538747	24.873851024756814
33	25.980052655583734	26.07971698227037	25.606975054960166	22.333255307185727
34	17.11789107388915	32.54703890769829	24.38687133649138	25.94819868192118
35	25.23341481837202	25.1455750728177	27.821550178454647	21.79945993035563
36	22.41264892335982	30.041192979577257	26.317415194828076	21.228742902234846
37	24.3284723847767	30.251622260135957	21.89067812766204	23.529227227425306
38	24.79300950068896	27.23587143543175	25.279023917025224	22.692095146854058
39	20.250439802022726	30.575229674389647	25.966780166557673	23.207550357029955
40	27.90166774857562	26.641263927064053	23.04634994485884	22.410718379501485
41	20.058109370135934	24.9870291584518	31.303527344947163	23.651334126465102
42	26.507573764874238	25.60070078742058	23.339309975361434	24.552415472343753
43	21.833727083841108	25.154262520180215	27.71078522458258	25.301225171396098
44	21.75554005757847	23.478067815179386	26.3623003395344	28.404091787707745
45	26.12411949101211	26.936395819407277	25.416092530967134	21.523392158613483
46	19.231112644820954	25.062320368926933	34.065170334297804	21.641396651954313
47	22.796827151168824	27.19943242010565	25.186116493842768	24.817623934882754
48	20.466178078191852	23.08544345799016	33.47466523162906	22.97371323218892
49	23.608620843599404	22.012785026701835	29.512947916339883	24.86564621335888
50	21.012280672118845	22.165297991510432	27.580232196162562	29.242189140208165
51	18.84524519113591	22.41482078520045	33.550921714033365	25.189012309630275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2526.0
1	2046.0
2	1566.0
3	1056.5
4	547.0
5	757.5
6	968.0
7	910.0
8	852.0
9	814.5
10	777.0
11	752.0
12	727.0
13	770.0
14	813.0
15	842.5
16	872.0
17	861.5
18	851.0
19	839.5
20	828.0
21	978.5
22	1129.0
23	1369.0
24	1609.0
25	2027.0
26	2875.5
27	3306.0
28	3810.0
29	4314.0
30	5054.5
31	5795.0
32	6713.0
33	7631.0
34	8729.5
35	9828.0
36	11241.0
37	12654.0
38	12892.0
39	13130.0
40	14688.0
41	16246.0
42	19161.5
43	22077.0
44	37003.5
45	51930.0
46	51792.5
47	51655.0
48	49021.5
49	46388.0
50	42654.0
51	38920.0
52	34821.5
53	30723.0
54	25822.0
55	20921.0
56	18511.0
57	16101.0
58	14677.0
59	13253.0
60	11609.5
61	9966.0
62	8438.0
63	6910.0
64	5983.5
65	5057.0
66	4209.0
67	3361.0
68	3056.0
69	2751.0
70	2252.5
71	1754.0
72	1526.5
73	1299.0
74	1134.0
75	733.0
76	497.0
77	352.5
78	208.0
79	168.0
80	128.0
81	90.5
82	53.0
83	45.0
84	37.0
85	22.0
86	7.0
87	5.5
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	414391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.04347491160824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37861030821354	26.682802536343868
2	8.428445613899067	5.738671536012549
3	3.031195601838777	3.0957729427012697
4	1.5559173624101674	2.1187533476698484
5	1.0296539224245291	1.7526498737849239
6	0.7766193820011573	1.586329346821506
7	0.5970336416284941	1.4227569860116025
8	0.5092812114465669	1.3870161715867724
9	0.46581995801910714	1.427231704873489
>10	4.929465857419162	35.621891127409704
>50	0.23111234063799868	4.933031671650713
>100	0.056178076647390454	3.592347105836234
>500	0.003555574471353826	0.7853341877035341
>1k	0.004977804259895357	3.574674665730698
>5k	0.002133344682812296	6.280736795863283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	9568	2.308930454570683	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	8273	1.9964236675024314	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGT	8103	1.9553996105127764	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	3818	0.9213520563911861	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	2246	0.5420001882280262	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2174	0.5246252935029959	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	1930	0.4657437058237269	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTG	1703	0.4109645238434233	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTAT	1514	0.3653554251902189	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGTC	1381	0.3332601335453714	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	872	0.21042928055869942	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	708	0.17085313146279724	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	623	0.15034110296796988	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	532	0.12838116657939	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	509	0.12283085298667201	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATG	493	0.11896976526999863	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	476	0.11486735957103315	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTAGCAGAGTCGTATGCCGT	443	0.1069038661553943	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.10666254817310221	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.1035254144033051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1998330079562538	0.0
2	0.0	0.0	0.0	1.53478236737767	0.0
3	0.0	0.0	0.0	2.407629509328147	0.0
4	0.0	0.0	0.0	6.27450885757654	0.0
5	0.0	0.0	0.0	6.82857494491917	0.0
6	0.0	0.0	0.0	8.83392737776641	0.0
7	0.0	0.0	0.0	10.45896267052132	0.0
8	0.0	0.0	0.0	11.513763571120029	0.0
9	0.0	0.0	0.0	14.857465533759179	0.0
10	0.0	0.0	0.0	16.50011703922141	0.0
11	0.0	0.0	0.0	20.634135393867144	0.0
12	0.0	0.0	0.0	21.580825838399	0.0
13	0.0	0.0	0.0	22.13561587968851	0.0
14	0.0	0.0	0.0	22.920864594066956	0.0
15	0.0	0.0	0.0	23.3098691815218	0.0
16	0.0	0.0	0.0	23.89506528858011	0.0
17	0.0	0.0	0.0	24.612744967916775	0.0
18	2.4131798229208646E-4	0.0	0.0	26.096367923048522	0.0
19	2.4131798229208646E-4	0.0	0.0	26.586967381048332	0.0
20	2.4131798229208646E-4	0.0	0.0	27.046678137314757	0.0
21	2.4131798229208646E-4	0.0	0.0	27.542586590924994	0.0
22	2.4131798229208646E-4	0.0	0.0	28.030772869101888	0.0
23	2.4131798229208646E-4	0.0	0.0	28.532714272269427	0.0
24	4.8263596458417293E-4	0.0	0.0	28.858252230381453	0.0
25	4.8263596458417293E-4	0.0	0.0	29.141318223610067	0.0
26	4.8263596458417293E-4	0.0	0.0	29.426797396661605	0.0
27	4.8263596458417293E-4	0.0	0.0	29.7624707100299	0.0
28	4.8263596458417293E-4	0.0	0.0	30.062911597983547	0.0
29	4.8263596458417293E-4	0.0	0.0	30.38482978636119	0.0
30	4.8263596458417293E-4	0.0	0.0	30.87180947462662	0.0
31	4.8263596458417293E-4	0.0	0.0	31.175387496350066	0.0
32	4.8263596458417293E-4	0.0	0.0	31.459901397472436	0.0
33	4.8263596458417293E-4	0.0	0.0	31.75551592578024	0.0
34	4.8263596458417293E-4	0.0	0.0	32.01131298700985	0.0
35	4.8263596458417293E-4	0.0	0.0	32.32743954381249	0.0
36	4.8263596458417293E-4	0.0	0.0	32.616779804580695	0.0
37	4.8263596458417293E-4	0.0	0.0	32.885849354836374	0.0
38	4.8263596458417293E-4	0.0	0.0	33.17687884148063	0.0
39	7.239539468762594E-4	0.0	0.0	33.46694305619572	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.028059E-4	45.0	26
CGGGTCA	20	7.028059E-4	45.0	6
CGGGTAT	80	0.0	45.0	6
CGGGTAG	35	1.2094097E-7	45.0	6
CTCACGG	50	2.1827873E-11	45.0	2
CGAACAG	25	3.8864724E-5	45.0	1
CGAGTAT	35	1.2094097E-7	45.0	16
CGAGTAG	35	1.2094097E-7	45.0	1
CGTGCGG	25	3.8864724E-5	45.0	2
TCCGAGT	35	1.2094097E-7	45.0	14
GCGAAAA	20	7.028059E-4	45.0	29
TCTTACG	20	7.028059E-4	45.0	1
GCCGGGT	20	7.028059E-4	45.0	4
ACGGGTA	70	0.0	45.0	5
TAGCCGT	25	3.8864724E-5	45.0	44
ACGGGCC	40	6.7975634E-9	45.0	5
TGCATCG	20	7.028059E-4	45.0	1
ATCCGCG	20	7.028059E-4	45.0	1
GTAGCCG	25	3.8864724E-5	45.0	43
CTAACCG	25	3.8864724E-5	45.0	1
>>END_MODULE