FastQCFastQC Report
Sat 18 Jun 2016
SRR3548695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439174
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC59811.3618747922235834No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGT57781.3156516551526274No Hit
AATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT57271.3040389458392345No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT38790.8832490083657046RNA PCR Primer, Index 27 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26840.6111472901401267No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT14510.3303929649751579No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT13130.2989703397742125No Hit
AACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTG12140.2764280216952734RNA PCR Primer, Index 27 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT10750.24477769631171242No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT9630.2192752758587712No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8150.18557564883167035No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTTC7480.17031973659642877No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT6880.15665772563949598No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6120.13935251176071445No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC5880.1338877073779413RNA PCR Primer, Index 27 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.11726559404700643No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC4940.11248389021207994No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTCTTTCGTATGCC4750.10815758674238456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG253.8868548E-545.0000041
TAACGTT253.8868548E-545.00000419
CTAACGT253.8868548E-545.00000418
ACGATAG253.8868548E-545.0000041
CTAAACG253.8868548E-545.0000041
TACGACA253.8868548E-545.00000421
CGGTCTA351.209628E-745.00000431
ACTCCGA351.209628E-745.00000413
TTACCGG351.209628E-745.0000042
TATCGCG253.8868548E-545.0000041
CAATACG253.8868548E-545.0000041
GATAGAA253.8868548E-545.0000049
TGATCGC207.0285215E-445.029
TACGCGG406.7993824E-945.02
TGCGCCC207.0285215E-445.033
ACGGCCC207.0285215E-445.011
TAACACG207.0285215E-445.01
CATCGCC207.0285215E-445.023
TTACGAG207.0285215E-445.01
ACGTAAG207.0285215E-445.01
Produced by FastQC (version 0.11.5)