SRR3548690_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302425
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	5378	1.778292138546747	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGT	4648	1.5369099776804167	No Hit
AATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	4256	1.4072910639001406	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	3553	1.174836736380921	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2770	0.9159295693147061	No Hit
AATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	1030	0.3405803091675622	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	1023	0.33826568570720017	No Hit
AACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	949	0.31379680912622965	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTAT	877	0.28998925353393407	No Hit
AAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	813	0.2688269818963379	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACGCACTC	618	0.20434818550053732	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.19442837067041416	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	583	0.19277506819872697	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	557	0.18417789534595352	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	548	0.1812019508969166	No Hit
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	481	0.15904769777630817	TruSeq Adapter, Index 16 (95% over 21bp)
GCCCAGGGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	431	0.14251467305943624	No Hit
GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTC	411	0.13590146317268742	No Hit
AATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	392	0.1296189137802761	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	371	0.12267504339918989	No Hit
GCCCAGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCAC	359	0.1187071174671406	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	346	0.11440853104075391	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAC	25	3.883966E-5	45.000004	10
ACAACAG	25	3.883966E-5	45.000004	1
TATCACG	25	3.883966E-5	45.000004	1
CTAGAAC	25	3.883966E-5	45.000004	22
GGTACAA	30	2.160179E-6	45.000004	8
ATCTAGT	25	3.883966E-5	45.000004	33
CATGCAA	25	3.883966E-5	45.000004	15
GACAATC	25	3.883966E-5	45.000004	17
GCTATCA	30	2.160179E-6	45.000004	9
TGGCGAT	25	3.883966E-5	45.000004	38
CGATTCG	25	3.883966E-5	45.000004	38
TACGGGT	25	3.883966E-5	45.000004	4
ACGGGTC	25	3.883966E-5	45.000004	5
ATCGTAG	30	2.160179E-6	45.000004	1
GACTTAG	25	3.883966E-5	45.000004	9
ATTCACG	25	3.883966E-5	45.000004	1
TATCTAG	25	3.883966E-5	45.000004	32
AACATAG	25	3.883966E-5	45.000004	1
TGATTGG	30	2.160179E-6	45.000004	2
CCATAGG	25	3.883966E-5	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content