##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302425
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97913862941225	31.0	31.0	33.0	28.0	34.0
2	31.277463834008433	31.0	31.0	34.0	30.0	34.0
3	31.462660163676944	31.0	31.0	34.0	30.0	34.0
4	35.42807307596925	37.0	35.0	37.0	33.0	37.0
5	34.69589815656774	35.0	35.0	37.0	32.0	37.0
6	34.84115731173018	35.0	35.0	37.0	32.0	37.0
7	34.75675622055055	35.0	35.0	37.0	32.0	37.0
8	35.19670000826651	36.0	35.0	37.0	33.0	37.0
9	36.57325287261305	38.0	35.0	39.0	32.0	39.0
10	36.034679672646114	37.0	35.0	39.0	31.0	39.0
11	36.28431181284616	37.0	35.0	39.0	32.0	39.0
12	35.9829544515169	37.0	35.0	39.0	32.0	39.0
13	35.68105150037199	37.0	35.0	39.0	30.0	39.0
14	36.6105050839051	38.0	35.0	40.0	31.0	41.0
15	36.750619161775646	38.0	35.0	40.0	32.0	41.0
16	36.54545755145904	38.0	35.0	40.0	31.0	41.0
17	36.4823708357444	38.0	35.0	40.0	31.0	41.0
18	36.3992527072828	38.0	35.0	40.0	31.0	41.0
19	36.39775150863851	38.0	35.0	40.0	30.0	41.0
20	36.37064726791767	38.0	35.0	40.0	30.0	41.0
21	36.492353476068445	38.0	35.0	40.0	31.0	41.0
22	36.383582706456146	38.0	35.0	40.0	30.0	41.0
23	36.28355129370918	38.0	35.0	40.0	30.0	41.0
24	35.919093990245514	38.0	34.0	40.0	30.0	41.0
25	35.993730677027365	38.0	34.0	40.0	30.0	41.0
26	36.029690005786556	38.0	34.0	40.0	30.0	41.0
27	35.87515251715301	38.0	34.0	40.0	30.0	41.0
28	35.650686947176986	38.0	34.0	40.0	29.0	41.0
29	35.226009754484586	37.0	34.0	40.0	27.0	41.0
30	35.24707613457882	37.0	34.0	40.0	27.0	41.0
31	35.16940398445896	37.0	34.0	40.0	27.0	41.0
32	35.15897495246755	37.0	34.0	40.0	27.0	41.0
33	35.11000082665124	37.0	34.0	40.0	27.0	41.0
34	35.033800115731175	37.0	34.0	40.0	26.0	41.0
35	35.02537157973051	37.0	34.0	40.0	26.0	41.0
36	35.071382987517566	37.0	34.0	40.0	27.0	41.0
37	34.90926014714392	37.0	33.0	40.0	26.0	41.0
38	34.81722741175498	37.0	33.0	40.0	26.0	41.0
39	34.702187319170044	37.0	33.0	40.0	25.0	41.0
40	34.57569314706125	37.0	33.0	40.0	25.0	41.0
41	34.642718029263456	37.0	33.0	40.0	25.0	41.0
42	34.6870364553195	37.0	33.0	40.0	26.0	41.0
43	34.7337753161941	37.0	33.0	40.0	26.0	41.0
44	34.66351988096222	37.0	33.0	40.0	26.0	41.0
45	34.43948747623378	37.0	33.0	40.0	25.0	41.0
46	34.31328097875506	36.0	33.0	40.0	25.0	41.0
47	34.23795321154005	36.0	33.0	39.0	25.0	41.0
48	34.31883938166487	36.0	33.0	39.0	25.0	41.0
49	34.21763412416301	36.0	33.0	39.0	24.0	41.0
50	34.10179383318178	36.0	33.0	39.0	24.0	40.0
51	32.77300487724229	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	5.0
12	6.0
13	4.0
14	11.0
15	32.0
16	70.0
17	113.0
18	223.0
19	461.0
20	783.0
21	1212.0
22	1705.0
23	2049.0
24	2379.0
25	2609.0
26	3133.0
27	3728.0
28	4723.0
29	6329.0
30	8345.0
31	11097.0
32	14897.0
33	20884.0
34	31129.0
35	34032.0
36	36900.0
37	45246.0
38	46717.0
39	23595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.498718690584443	38.23791022567578	18.130776225510456	24.132594858229314
2	21.430106638009423	37.35174010085145	17.594114243200792	23.62403901793833
3	18.052409688352483	28.88154087790361	25.719765231049017	27.346284202694882
4	23.335703066876086	30.66776886831446	18.335455071505333	27.661072993304124
5	15.600562122840373	39.16508225179797	18.651566504091925	26.582789121269734
6	20.420269488302885	34.32388195420352	24.735388939406462	20.520459618087127
7	72.11903777796148	12.501942630404233	8.778044143175993	6.600975448458296
8	73.22013722410516	6.499462676696702	7.012978424402745	13.267421674795404
9	70.95941142432008	8.590890303381004	12.834587087707696	7.615111184591221
10	35.946763660411676	29.646689261800447	15.062908159047698	19.343638918740183
11	27.947094320906007	27.07415061585517	25.327271224270483	19.65148383896834
12	27.1759940481111	22.164503595932874	33.185748532694056	17.473753823261966
13	25.783252045961806	29.848061502851948	25.065718773249568	19.302967677936678
14	15.387286103992725	32.14350665454245	32.63751343308258	19.831693808382244
15	13.60767132346863	27.67992064148136	33.769033644705296	24.943374390344715
16	14.865503843928247	28.464247334049762	32.629577581218484	24.040671240803505
17	14.365875836984376	25.81036620649748	29.3292551872365	30.494502769281638
18	20.470529883442175	24.220219889228733	28.41795486484252	26.891295362486566
19	21.38943539720592	29.976357774654872	28.80747292717203	19.82673390096718
20	22.08316111432587	29.73795155823758	24.03471935190543	24.144167975531122
21	18.707117467140613	30.126146978589734	27.578077209225427	23.588658345044227
22	17.542531206084153	30.665123584359762	24.79788377283624	26.994461436719845
23	18.862197238984873	29.760436471852525	22.34802017029015	29.029346118872446
24	15.063238819542036	32.57997850706787	25.91683888567413	26.439943787715965
25	17.812019508969165	28.44440770438952	27.011986442919735	26.731586343721585
26	21.614284533355377	29.77796147805241	24.038687277837482	24.569066710754733
27	17.4254773910887	28.578655865090518	26.39001405307101	27.60585269074977
28	19.920641481359013	29.33520707613458	27.838637678763327	22.905513763743077
29	20.49698272298917	30.24915268248326	27.790361246590063	21.463503347937504
30	21.610647267917667	30.06034554021658	22.988178887327436	25.340828304538316
31	24.00363726543771	31.119120443085063	19.593287591964952	25.28395469951227
32	24.66793419856163	27.226915764239067	21.476399107216665	26.62875092998264
33	20.94899561874845	28.346862858559973	22.572869306439614	28.13127221625196
34	18.021658262379102	30.183681904604448	24.510539803257007	27.284120029759446
35	18.141357361329256	29.056790939902456	25.13350417458874	27.66834752417955
36	21.21385467471274	27.087707696123005	28.535339340332317	23.163098288831943
37	20.865338513681078	30.783500041332562	20.178556666942217	28.172604778044143
38	22.45317020748946	27.770521616929816	22.686616516491693	27.089691659089034
39	22.637017442341076	24.652723815822107	23.933867901132512	28.77639084070431
40	22.877407621724394	26.009754484582952	21.636769446970323	29.476068446722326
41	18.255104571381334	23.84359758617839	27.940150450524925	29.96114739191535
42	24.074398611225924	25.055137637430768	22.636025460858065	28.234438290485244
43	20.483094982226998	22.96569397371249	28.799867735802266	27.751343308258246
44	20.93907580391833	24.31644209308093	25.529635446804992	29.214846656195753
45	24.92353476068447	25.108373977019095	25.76242043481855	24.20567082747789
46	20.61866578490535	23.926924030751426	32.28238406216417	23.17202612217905
47	20.795899809870217	26.727618417789532	24.123997685376537	28.35248408696371
48	21.34016698354964	22.52789947920972	31.956022154253123	24.17591138298752
49	23.12705629494916	19.614780524096883	28.56972803174341	28.68843514921055
50	20.395469951227575	22.701496238736876	26.642308010250478	30.26072579978507
51	19.85021079606514	21.981979003058612	31.049351078779864	27.11845912209639
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3156.0
1	2488.5
2	1821.0
3	1202.5
4	584.0
5	652.5
6	721.0
7	689.0
8	657.0
9	662.5
10	668.0
11	647.0
12	626.0
13	651.0
14	676.0
15	664.0
16	652.0
17	698.5
18	745.0
19	706.0
20	667.0
21	749.0
22	831.0
23	883.0
24	935.0
25	1191.0
26	1364.0
27	1281.0
28	1685.5
29	2090.0
30	2399.5
31	2709.0
32	3100.5
33	3492.0
34	4075.5
35	4659.0
36	5033.5
37	5408.0
38	5926.5
39	6445.0
40	7969.5
41	9494.0
42	12123.5
43	14753.0
44	24266.0
45	33779.0
46	34926.5
47	36074.0
48	36040.5
49	36007.0
50	33806.5
51	31606.0
52	28302.5
53	24999.0
54	21383.5
55	17768.0
56	15196.0
57	12624.0
58	11252.0
59	9880.0
60	9125.0
61	8370.0
62	7341.5
63	6313.0
64	5688.5
65	5064.0
66	4687.0
67	4310.0
68	3797.0
69	3284.0
70	3141.0
71	2998.0
72	2451.0
73	1904.0
74	1512.5
75	922.0
76	723.0
77	615.0
78	507.0
79	392.5
80	278.0
81	237.0
82	196.0
83	115.0
84	34.0
85	33.5
86	33.0
87	31.0
88	29.0
89	17.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.42482311925439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7546699739799	26.76690760550258
2	9.17786264210194	6.318925961343441
3	3.221288955393695	3.326769075163071
4	1.6429979494177138	2.262396551760099
5	1.0255468482552905	1.76521344258486
6	0.7928572748549672	1.6376382867417785
7	0.5781649817882991	1.3932259062266394
8	0.44236095591454655	1.2182558129778045
9	0.40641286908593316	1.259162201850475
>10	4.633306390417168	35.19672865024589
>50	0.2526350251507945	5.387275431099651
>100	0.0569177724648035	3.7767990787067296
>500	0.007988459293305755	1.9019777284503807
>1k	0.005991344469979316	5.940028040416154
>5k	9.985574116632193E-4	1.8486962269304443
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	5378	1.778292138546747	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGT	4648	1.5369099776804167	No Hit
AATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	4256	1.4072910639001406	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	3553	1.174836736380921	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2770	0.9159295693147061	No Hit
AATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	1030	0.3405803091675622	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	1023	0.33826568570720017	No Hit
AACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	949	0.31379680912622965	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTAT	877	0.28998925353393407	No Hit
AAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	813	0.2688269818963379	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACGCACTC	618	0.20434818550053732	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.19442837067041416	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	583	0.19277506819872697	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	557	0.18417789534595352	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	548	0.1812019508969166	No Hit
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	481	0.15904769777630817	TruSeq Adapter, Index 16 (95% over 21bp)
GCCCAGGGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	431	0.14251467305943624	No Hit
GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTC	411	0.13590146317268742	No Hit
AATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	392	0.1296189137802761	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	371	0.12267504339918989	No Hit
GCCCAGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCAC	359	0.1187071174671406	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	346	0.11440853104075391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4049764404397784	0.0
2	0.0	0.0	0.0	1.8434322559312226	0.0
3	0.0	0.0	0.0	2.6185004546581796	0.0
4	0.0	0.0	0.0	6.063652145159957	0.0
5	0.0	0.0	0.0	6.6132098867487805	0.0
6	0.0	0.0	0.0	8.829957840786973	0.0
7	0.0	0.0	0.0	10.619492436141192	0.0
8	0.0	0.0	0.0	12.26353641398694	0.0
9	0.0	0.0	0.0	15.476895097958172	0.0
10	0.0	0.0	0.0	18.150615855170702	0.0
11	0.0	0.0	0.0	22.07357195999008	0.0
12	0.0	0.0	0.0	23.326444573034635	0.0
13	0.0	0.0	0.0	23.999338679011323	0.0
14	0.0	0.0	0.0	24.8240059518889	0.0
15	0.0	0.0	0.0	25.379846242870133	0.0
16	0.0	0.0	0.0	26.1532611391254	0.0
17	0.0	0.0	0.0	26.97858973299165	0.0
18	0.0	0.0	0.0	28.849797470447218	0.0
19	0.0	0.0	0.0	29.33487641564024	0.0
20	0.0	0.0	0.0	30.106638009423826	0.0
21	0.0	0.0	0.0	30.60163676944697	0.0
22	0.0	0.0	0.0	31.07282797387782	0.0
23	0.0	0.0	0.0	31.578077209225427	0.0
24	0.0	0.0	0.0	31.906423080102506	0.0
25	0.0	0.0	0.0	32.18186327188559	0.0
26	0.0	0.0	0.0	32.4212614697859	0.0
27	0.0	0.0	0.0	32.830949822269986	0.0
28	0.0	0.0	0.0	33.1384640820038	0.0
29	0.0	0.0	0.0	33.449946267669674	0.0
30	0.0	0.0	0.0	34.02860213276019	0.0
31	0.0	0.0	0.0	34.30999421344135	0.0
32	0.0	0.0	0.0	34.60295941142432	0.0
33	0.0	0.0	0.0	34.90419112176573	0.0
34	0.0	0.0	0.0	35.16342894932628	0.0
35	0.0	0.0	0.0	35.47689509795817	0.0
36	0.0	0.0	0.0	35.717946598330165	0.0
37	0.0	0.0	0.0	35.96528064809457	0.0
38	0.0	0.0	0.0	36.23212366702488	0.0
39	0.0	0.0	0.0	36.51649169215508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAC	25	3.883966E-5	45.000004	10
ACAACAG	25	3.883966E-5	45.000004	1
TATCACG	25	3.883966E-5	45.000004	1
CTAGAAC	25	3.883966E-5	45.000004	22
GGTACAA	30	2.160179E-6	45.000004	8
ATCTAGT	25	3.883966E-5	45.000004	33
CATGCAA	25	3.883966E-5	45.000004	15
GACAATC	25	3.883966E-5	45.000004	17
GCTATCA	30	2.160179E-6	45.000004	9
TGGCGAT	25	3.883966E-5	45.000004	38
CGATTCG	25	3.883966E-5	45.000004	38
TACGGGT	25	3.883966E-5	45.000004	4
ACGGGTC	25	3.883966E-5	45.000004	5
ATCGTAG	30	2.160179E-6	45.000004	1
GACTTAG	25	3.883966E-5	45.000004	9
ATTCACG	25	3.883966E-5	45.000004	1
TATCTAG	25	3.883966E-5	45.000004	32
AACATAG	25	3.883966E-5	45.000004	1
TGATTGG	30	2.160179E-6	45.000004	2
CCATAGG	25	3.883966E-5	45.000004	1
>>END_MODULE