SRR3548687_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477499
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCC	8886	1.8609463056467133	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGT	7954	1.6657626508118342	No Hit
AATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	7854	1.644820198576332	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	6078	1.2728822468738155	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5003	1.0477508853421682	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1948	0.40795896954758015	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	1935	0.40523645075696496	No Hit
AACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTG	1715	0.3591630558388604	No Hit
AAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	1681	0.35204262207878967	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTAT	1461	0.3059692271606852	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.24209474784240387	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1048	0.21947689942806164	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTTC	1040	0.21780150324922148	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.192879985088974	No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	817	0.17109983476405186	No Hit
AATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	685	0.14345579781318915	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCC	681	0.14261809972376907	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	627	0.13130917551659793	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCCGT	541	0.11329866659406616	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	534	0.11183269493758102	No Hit
CTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCCGTCTTCTGCT	520	0.10890075162461074	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	45	3.8380676E-10	45.000004	6
GTACCGG	35	1.2099372E-7	45.000004	2
GAGTCGC	30	2.1628257E-6	45.000004	25
TAGACGG	35	1.2099372E-7	45.000004	2
CGCATAG	30	2.1628257E-6	45.000004	1
CTAACGG	45	3.8380676E-10	45.000004	2
CCCCGTT	30	2.1628257E-6	45.000004	15
CCGTAGT	45	3.8380676E-10	45.000004	27
CGTAGTG	45	3.8380676E-10	45.000004	28
GAGCGTA	30	2.1628257E-6	45.000004	9
TCTCGGT	30	2.1628257E-6	45.000004	43
GAACGCA	35	1.2099372E-7	45.000004	8
TCGAGGG	45	3.8380676E-10	45.000004	3
CGCACAG	45	3.8380676E-10	45.000004	1
CACCGTA	45	3.8380676E-10	45.000004	25
ATTGCGG	30	2.1628257E-6	45.000004	2
CTTACCG	30	2.1628257E-6	45.000004	1
TGATCGG	20	7.0291397E-4	45.0	2
TTAGGAC	20	7.0291397E-4	45.0	29
AGGTACG	20	7.0291397E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content