##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477499
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.925241728254928	31.0	31.0	33.0	28.0	34.0
2	31.213893641662075	31.0	31.0	34.0	28.0	34.0
3	31.429969486847092	31.0	31.0	34.0	30.0	34.0
4	35.38580813781809	37.0	35.0	37.0	33.0	37.0
5	34.60054157181481	35.0	35.0	37.0	32.0	37.0
6	34.73407693000404	35.0	35.0	37.0	32.0	37.0
7	34.65832179753256	35.0	35.0	37.0	32.0	37.0
8	35.05362733743945	35.0	35.0	37.0	32.0	37.0
9	36.588302802728386	38.0	35.0	39.0	32.0	39.0
10	35.94671611877721	37.0	35.0	39.0	31.0	39.0
11	36.19184542794854	37.0	35.0	39.0	32.0	39.0
12	35.86708663264216	37.0	35.0	39.0	31.0	39.0
13	35.625278796395385	37.0	35.0	39.0	30.0	39.0
14	36.548487012538246	38.0	35.0	40.0	31.0	41.0
15	36.64788407933839	38.0	35.0	40.0	31.0	41.0
16	36.45457896246903	38.0	35.0	40.0	31.0	41.0
17	36.43037158193002	38.0	35.0	40.0	31.0	41.0
18	36.23210519812607	38.0	35.0	40.0	30.0	41.0
19	36.27078800165027	38.0	34.0	40.0	30.0	41.0
20	36.230794200616124	38.0	34.0	40.0	30.0	41.0
21	36.380702367963075	38.0	35.0	40.0	31.0	41.0
22	36.327135763635106	38.0	35.0	40.0	30.0	41.0
23	36.16780768127263	38.0	34.0	40.0	30.0	41.0
24	35.737714633957346	38.0	34.0	40.0	29.0	41.0
25	35.97946173709264	38.0	34.0	40.0	30.0	41.0
26	35.833522164444325	38.0	34.0	40.0	29.0	41.0
27	35.68858154676764	38.0	34.0	40.0	29.0	41.0
28	35.56903574667172	38.0	34.0	40.0	28.0	41.0
29	35.17338465630294	37.0	34.0	40.0	27.0	41.0
30	35.25293037262905	38.0	34.0	40.0	27.0	41.0
31	35.04691737574319	37.0	34.0	40.0	26.0	41.0
32	34.861494997895285	37.0	33.0	40.0	25.0	41.0
33	34.807578654614986	37.0	33.0	40.0	25.0	41.0
34	34.811032064988616	37.0	33.0	40.0	25.0	41.0
35	34.76959323475023	37.0	33.0	40.0	25.0	41.0
36	34.7709419286742	37.0	33.0	40.0	25.0	41.0
37	34.48450363246834	37.0	33.0	40.0	25.0	41.0
38	34.33997976959114	37.0	33.0	40.0	24.0	41.0
39	34.29357548392772	37.0	33.0	40.0	24.0	41.0
40	34.09667664225475	37.0	33.0	40.0	23.0	41.0
41	34.4989329820586	37.0	33.0	40.0	24.0	41.0
42	34.581284986984265	37.0	33.0	40.0	25.0	41.0
43	34.71298159786722	37.0	33.0	40.0	26.0	41.0
44	34.521418893023856	37.0	33.0	40.0	24.0	41.0
45	34.224503087964585	37.0	33.0	40.0	24.0	41.0
46	34.12199397276225	37.0	33.0	40.0	23.0	41.0
47	34.07625356283469	37.0	33.0	40.0	23.0	41.0
48	34.221118787683324	37.0	33.0	40.0	24.0	41.0
49	34.12387251072777	36.0	33.0	40.0	24.0	40.0
50	34.09582219020354	36.0	33.0	40.0	24.0	40.0
51	32.72882456298338	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	14.0
12	5.0
13	17.0
14	28.0
15	55.0
16	93.0
17	184.0
18	402.0
19	841.0
20	1385.0
21	2087.0
22	2804.0
23	3453.0
24	3884.0
25	4394.0
26	5195.0
27	6387.0
28	8112.0
29	10847.0
30	14063.0
31	18902.0
32	25024.0
33	33561.0
34	49322.0
35	54504.0
36	56852.0
37	67691.0
38	70011.0
39	37370.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.111240023539317	40.10751854977707	19.190616106002317	23.590625320681298
2	19.584962481596822	40.16134065202231	19.525695341770348	20.728001524610523
3	19.094909099286074	30.01179060060859	27.419324438375785	23.473975861729553
4	24.281097970885803	31.149803455085767	18.386216515636683	26.182882058391744
5	15.353540007413628	40.10500545550881	19.779517862864633	24.76193667421293
6	19.536794841455166	34.44928680478912	25.918797735702064	20.09512061805365
7	69.29291998517274	13.854688700918746	9.594784491695272	7.257606822213241
8	71.16706003572783	6.866192389931706	7.398758950280524	14.567988624059947
9	68.99867853126393	9.84881643731191	13.788719976376914	7.363785055047235
10	33.52383984050228	33.1447814550397	15.3648489316208	17.96652977283722
11	25.246335594420096	24.340365110712273	30.3357703366918	20.077528958175826
12	25.45764493747631	22.073763505263884	35.90541550872358	16.563176048536228
13	23.07544099568795	31.796715804640428	25.686127091365634	19.441716108305986
14	13.750814137830655	32.33849704397287	35.04174877853147	18.868940039665006
15	12.026203196237061	30.350430053256655	33.362164109244205	24.261202641262074
16	13.913536991700507	28.192729199432883	34.33117137418089	23.562562434685727
17	12.696989941340192	28.063514269139834	30.634200281047708	28.605295508472267
18	17.71291667626529	26.71775228848647	28.47985021958161	27.08948081566663
19	17.93930458493107	32.76677019218888	29.91336107510173	19.38056414777832
20	20.347686592013805	32.079020060775	26.364034270228835	21.20925907698236
21	16.64547988582175	31.503940322388107	27.146653710269554	24.70392608152059
22	15.917101397070986	32.07587869293967	26.521102661995105	25.485917247994237
23	18.683389912858456	30.605927970529784	22.40256000536127	28.308122111250494
24	13.88526468118258	34.677350109633736	27.61555521582244	23.82182999336124
25	15.984117244224597	27.665817101187645	27.84403736971177	28.50602828487599
26	20.13595839991288	31.464777936707723	22.983503630374095	25.415760033005302
27	16.224536595888157	29.22351669846429	27.148957380015453	27.402989325632092
28	18.39249925130733	28.40131602369848	26.684244364909667	26.52194036008452
29	17.377628015974903	31.393364174584658	25.45010565467153	25.77890215476891
30	23.37303324195443	28.824144134333267	22.589366679301946	25.213455944410356
31	21.354180846452035	33.495358105462	18.036477563303798	27.113983484782167
32	22.23816175531258	29.270008942427104	20.645907111847357	27.845922190412963
33	22.104130061005364	30.562786518924646	20.80506974883717	26.52801367123282
34	15.522336172431775	27.4844554648282	22.799210050701678	34.193998312038346
35	19.712083166666318	27.368224854921163	25.739111495521456	27.180580482891063
36	20.32527816812182	28.688437043847216	25.90120607582424	25.085078712206727
37	20.722137637984584	32.231481113049455	22.190203539693275	24.85617770927269
38	19.296794338836314	34.88928772625702	25.48151933302478	20.332398601881888
39	17.56799490679562	29.69576899637486	26.657856875092932	26.07837922173659
40	20.640880923310835	29.44550669216061	24.559842010140333	25.35377037438822
41	17.077313250917804	21.81365824849895	31.804883361012276	29.304145139570974
42	23.320258262320966	23.843191294641457	26.53680950117173	26.299740941865846
43	19.716062232591064	24.429161108190804	28.780164984638713	27.074611674579423
44	22.12151229636083	23.23439420815541	26.039845109623265	28.604248385860494
45	24.95314126312306	28.862887670968945	24.877748435075254	21.306222630832735
46	17.576162463167464	25.714399401883565	33.6932642790875	23.016173855861478
47	19.801926286756622	27.691995166482027	24.486752851838432	28.01932569492292
48	19.51983145514441	21.56674673664238	34.66981082682896	24.243610981384254
49	21.994391611291334	20.085277665502964	31.640485110963585	26.27984561224212
50	18.647159470491037	21.99690470555959	27.459324522145597	31.896611301803773
51	17.176580474514083	21.770097947849106	34.71127688225525	26.34204469538156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5739.0
1	4433.5
2	3128.0
3	2066.5
4	1005.0
5	1170.5
6	1336.0
7	1268.0
8	1200.0
9	1193.0
10	1186.0
11	1237.0
12	1288.0
13	1327.0
14	1366.0
15	1399.0
16	1432.0
17	1467.5
18	1503.0
19	1474.0
20	1445.0
21	1392.0
22	1339.0
23	1649.0
24	1959.0
25	2283.0
26	3013.5
27	3420.0
28	3421.5
29	3423.0
30	4462.0
31	5501.0
32	6572.0
33	7643.0
34	8665.0
35	9687.0
36	10117.5
37	10548.0
38	11787.0
39	13026.0
40	14507.5
41	15989.0
42	22305.0
43	28621.0
44	44222.5
45	59824.0
46	61485.5
47	63147.0
48	61537.5
49	59928.0
50	53826.5
51	47725.0
52	42217.5
53	36710.0
54	31412.0
55	26114.0
56	22092.0
57	18070.0
58	15146.0
59	12222.0
60	11001.0
61	9780.0
62	8228.0
63	6676.0
64	5792.5
65	4909.0
66	3840.5
67	2772.0
68	2373.5
69	1975.0
70	1601.5
71	1228.0
72	987.5
73	747.0
74	579.5
75	357.0
76	302.0
77	308.0
78	314.0
79	190.0
80	66.0
81	78.5
82	91.0
83	60.5
84	30.0
85	24.0
86	18.0
87	19.5
88	21.0
89	12.0
90	3.0
91	2.5
92	2.0
93	4.0
94	6.0
95	10.0
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.050288141543184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4778946629059	28.652010034809745
2	9.045922646986018	6.522162358599139
3	3.249243691390389	3.514085139501448
4	1.5818557779872395	2.281054263792198
5	0.956787236793445	1.72462277882773
6	0.6445163720823784	1.394100055530708
7	0.5106540688660881	1.2886458426291736
8	0.4175283091936645	1.204161268294635
9	0.3150988458298795	1.0223463766711365
>10	3.29389340783359	26.43079691651675
>50	0.42139262172220315	9.938745819823376
>100	0.07295578630924571	4.857857640106743
>500	0.0046691703237917255	1.1206232584549771
>1k	0.0046691703237917255	2.52150753460842
>5k	0.0029182314523698285	7.527280711833797
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCC	8886	1.8609463056467133	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGT	7954	1.6657626508118342	No Hit
AATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	7854	1.644820198576332	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	6078	1.2728822468738155	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5003	1.0477508853421682	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1948	0.40795896954758015	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	1935	0.40523645075696496	No Hit
AACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTG	1715	0.3591630558388604	No Hit
AAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	1681	0.35204262207878967	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTAT	1461	0.3059692271606852	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.24209474784240387	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1048	0.21947689942806164	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTTC	1040	0.21780150324922148	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.192879985088974	No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	817	0.17109983476405186	No Hit
AATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	685	0.14345579781318915	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCC	681	0.14261809972376907	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	627	0.13130917551659793	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCCGT	541	0.11329866659406616	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	534	0.11183269493758102	No Hit
CTGTCTCTTATACACATCTGACGCCCGACACTTCGTATGCCGTCTTCTGCT	520	0.10890075162461074	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0942452235502064E-4	0.0	0.0	1.6883804992261764	0.0
2	2.0942452235502064E-4	0.0	0.0	2.2094287108454678	0.0
3	2.0942452235502064E-4	0.0	0.0	3.134247401565239	0.0
4	2.0942452235502064E-4	0.0	0.0	7.483575881834308	0.0
5	2.0942452235502064E-4	0.0	0.0	8.083995987426151	0.0
6	2.0942452235502064E-4	0.0	0.0	10.66222128213881	0.0
7	2.0942452235502064E-4	0.0	0.0	12.828717965901498	0.0
8	2.0942452235502064E-4	0.0	0.0	14.540972860676147	0.0
9	2.0942452235502064E-4	0.0	0.0	18.1910328608018	0.0
10	2.0942452235502064E-4	0.0	0.0	20.923394603967758	0.0
11	2.0942452235502064E-4	0.0	0.0	25.40298513714165	0.0
12	2.0942452235502064E-4	0.0	0.0	26.78330216398359	0.0
13	2.0942452235502064E-4	0.0	0.0	27.48906280432001	0.0
14	2.0942452235502064E-4	0.0	0.0	28.351263562855628	0.0
15	2.0942452235502064E-4	0.0	0.0	28.835871907585148	0.0
16	2.0942452235502064E-4	0.0	0.0	29.670638053692258	0.0
17	2.0942452235502064E-4	0.0	0.0	30.680692525010524	0.0
18	2.0942452235502064E-4	0.0	0.0	32.5320053026289	0.0
19	2.0942452235502064E-4	0.0	0.0	33.09137820183916	0.0
20	2.0942452235502064E-4	0.0	0.0	33.71881407081481	0.0
21	2.0942452235502064E-4	0.0	0.0	34.27567387575681	0.0
22	2.0942452235502064E-4	0.0	0.0	34.81096295489624	0.0
23	2.0942452235502064E-4	0.0	0.0	35.379341108567765	0.0
24	2.0942452235502064E-4	0.0	0.0	35.75421100358325	0.0
25	2.0942452235502064E-4	0.0	0.0	36.06101792883336	0.0
26	2.0942452235502064E-4	0.0	0.0	36.35588765630923	0.0
27	2.0942452235502064E-4	0.0	0.0	36.75819216375322	0.0
28	2.0942452235502064E-4	0.0	0.0	37.0888734845518	0.0
29	2.0942452235502064E-4	0.0	0.0	37.42060192796215	0.0
30	4.188490447100413E-4	0.0	0.0	38.0335875048953	0.0
31	4.188490447100413E-4	0.0	0.0	38.363012278559744	0.0
32	4.188490447100413E-4	0.0	0.0	38.69097108056771	0.0
33	4.188490447100413E-4	0.0	0.0	39.00427016601082	0.0
34	4.188490447100413E-4	0.0	0.0	39.31086766673857	0.0
35	4.188490447100413E-4	0.0	0.0	39.655789855057286	0.0
36	4.188490447100413E-4	0.0	0.0	39.968041817888626	0.0
37	4.188490447100413E-4	0.0	0.0	40.269613130079854	0.0
38	4.188490447100413E-4	0.0	0.0	40.56427343303337	0.0
39	4.188490447100413E-4	0.0	0.0	40.858305462419814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	45	3.8380676E-10	45.000004	6
GTACCGG	35	1.2099372E-7	45.000004	2
GAGTCGC	30	2.1628257E-6	45.000004	25
TAGACGG	35	1.2099372E-7	45.000004	2
CGCATAG	30	2.1628257E-6	45.000004	1
CTAACGG	45	3.8380676E-10	45.000004	2
CCCCGTT	30	2.1628257E-6	45.000004	15
CCGTAGT	45	3.8380676E-10	45.000004	27
CGTAGTG	45	3.8380676E-10	45.000004	28
GAGCGTA	30	2.1628257E-6	45.000004	9
TCTCGGT	30	2.1628257E-6	45.000004	43
GAACGCA	35	1.2099372E-7	45.000004	8
TCGAGGG	45	3.8380676E-10	45.000004	3
CGCACAG	45	3.8380676E-10	45.000004	1
CACCGTA	45	3.8380676E-10	45.000004	25
ATTGCGG	30	2.1628257E-6	45.000004	2
CTTACCG	30	2.1628257E-6	45.000004	1
TGATCGG	20	7.0291397E-4	45.0	2
TTAGGAC	20	7.0291397E-4	45.0	29
AGGTACG	20	7.0291397E-4	45.0	1
>>END_MODULE