SRR3548685_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262961
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	3016	1.1469381391156863	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2928	1.1134731005738494	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	2442	0.9286548195359768	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	2292	0.8716121402033001	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGT	2005	0.7624704804134453	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	834	0.3171572970896825	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	635	0.24148067584166474	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	556	0.21143819805978836	No Hit
AACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	551	0.20953677541536578	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.20269165389544458	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	508	0.19318454067333177	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	452	0.17188860705579914	TruSeq Adapter, Index 14 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	447	0.1699871844113766	No Hit
ACTTACGGGACTGTGACCGAAGGCAAGTCAGTTCTCGGCCTCGCATCTGTA	417	0.15857864854484124	No Hit
GAATGAGGATAGACAGAGTGAGGGAGTTTCAGGTAGAGAGAGGAAGAAATG	330	0.12549389453188875	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTAT	325	0.12359247188746621	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	287	0.10914165978985477	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACTC	282	0.1072402371454322	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.1068599526165477	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTG	30	2.159095E-6	45.000004	5
GCAAGTC	60	0.0	45.000004	23
CGTAGCG	30	2.159095E-6	45.000004	1
CTCGCAG	30	2.159095E-6	45.000004	1
GTAACGG	30	2.159095E-6	45.000004	2
TACAACG	30	2.159095E-6	45.000004	1
GCGAGAC	140	0.0	45.0	21
CCTTCAT	25	3.8825747E-5	45.0	32
AGCGTTG	25	3.8825747E-5	45.0	1
CGGGTAC	35	1.2071541E-7	45.0	6
CTCACGG	20	7.0233556E-4	45.0	2
GTACCGG	35	1.2071541E-7	45.0	2
ACCGGGT	35	1.2071541E-7	45.0	4
ATAGGCG	20	7.0233556E-4	45.0	1
TTTACGG	20	7.0233556E-4	45.0	2
TTAGCGG	45	3.8198777E-10	45.0	2
ACCGCGC	20	7.0233556E-4	45.0	35
GGCAATA	25	3.8825747E-5	45.0	8
ATTACGG	20	7.0233556E-4	45.0	2
GGCCGGT	20	7.0233556E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content