##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262961
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.043150885492526	31.0	31.0	33.0	28.0	34.0
2	31.319458018489435	31.0	31.0	34.0	30.0	34.0
3	31.496651594723172	31.0	31.0	34.0	30.0	34.0
4	35.453778316936734	37.0	35.0	37.0	33.0	37.0
5	34.73457280737448	35.0	35.0	37.0	32.0	37.0
6	34.85322538323173	35.0	35.0	37.0	32.0	37.0
7	34.86801845140534	35.0	35.0	37.0	32.0	37.0
8	35.194500325143274	37.0	35.0	37.0	32.0	37.0
9	36.609592296956585	39.0	35.0	39.0	32.0	39.0
10	36.01602899289248	37.0	35.0	39.0	31.0	39.0
11	36.276622769155885	37.0	35.0	39.0	32.0	39.0
12	36.04297595460924	37.0	35.0	39.0	32.0	39.0
13	35.79948357360978	37.0	35.0	39.0	30.0	39.0
14	36.77298915048239	38.0	35.0	40.0	31.0	41.0
15	36.85505455181567	38.0	35.0	40.0	32.0	41.0
16	36.70113058590437	38.0	35.0	40.0	31.0	41.0
17	36.65758420450181	38.0	35.0	40.0	31.0	41.0
18	36.527230273690776	38.0	35.0	40.0	31.0	41.0
19	36.59989504147003	38.0	35.0	40.0	31.0	41.0
20	36.53472568175509	38.0	35.0	40.0	31.0	41.0
21	36.58021151425496	38.0	35.0	40.0	31.0	41.0
22	36.54915747962626	38.0	35.0	40.0	31.0	41.0
23	36.44557557964869	38.0	35.0	40.0	31.0	41.0
24	36.07134518046402	38.0	34.0	40.0	30.0	41.0
25	36.207140982883395	38.0	35.0	40.0	30.0	41.0
26	36.088594886694224	38.0	35.0	40.0	30.0	41.0
27	35.938154327067515	38.0	34.0	40.0	30.0	41.0
28	35.826518000768175	38.0	34.0	40.0	29.0	41.0
29	35.40390019812824	38.0	34.0	40.0	27.0	41.0
30	35.52213446100372	38.0	34.0	40.0	28.0	41.0
31	35.28637706732177	38.0	34.0	40.0	27.0	41.0
32	35.2267750731097	38.0	34.0	40.0	27.0	41.0
33	35.16471644084104	38.0	34.0	40.0	27.0	41.0
34	35.06670190636634	38.0	34.0	40.0	26.0	41.0
35	35.05962100843851	38.0	34.0	40.0	26.0	41.0
36	34.911716946619464	37.0	33.0	40.0	26.0	41.0
37	34.75067785717274	37.0	33.0	40.0	25.0	41.0
38	34.63647080745814	37.0	33.0	40.0	25.0	41.0
39	34.64404607527352	37.0	33.0	40.0	25.0	41.0
40	34.500686413574634	37.0	33.0	40.0	24.0	41.0
41	34.62377690988397	37.0	33.0	40.0	25.0	41.0
42	34.67389460794566	37.0	33.0	40.0	25.0	41.0
43	34.671882902787864	37.0	33.0	40.0	25.0	41.0
44	34.51071071375603	37.0	33.0	40.0	24.0	41.0
45	34.21651879936569	37.0	33.0	40.0	23.0	41.0
46	34.124162898680794	37.0	33.0	40.0	23.0	41.0
47	34.09208970151467	36.0	33.0	40.0	23.0	41.0
48	34.136293975152206	36.0	33.0	40.0	23.0	41.0
49	34.03454124375858	36.0	33.0	39.0	24.0	41.0
50	33.9373405181757	36.0	33.0	39.0	23.0	40.0
51	32.525758572563994	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	9.0
13	3.0
14	17.0
15	27.0
16	55.0
17	108.0
18	210.0
19	431.0
20	755.0
21	1126.0
22	1539.0
23	1993.0
24	2225.0
25	2492.0
26	2817.0
27	3431.0
28	4339.0
29	5409.0
30	7139.0
31	9473.0
32	12367.0
33	17193.0
34	25810.0
35	29038.0
36	30679.0
37	38435.0
38	42197.0
39	23639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.85754921832515	36.15060788481942	19.424173166363072	25.567669730492355
2	21.965234388369378	35.5200961359289	19.42493373542084	23.08973574028088
3	18.544194766524313	32.8881469115192	23.703895254429362	24.863763067527124
4	20.741859058947902	33.81071717859302	18.783774019721555	26.663649742737515
5	16.46936237693042	37.676689699232966	18.935507546746475	26.918440377090143
6	19.921585330144016	35.06223356315195	22.710211780454138	22.3059693262499
7	75.41688691478964	10.250949760610888	8.062032012351642	6.270131312247824
8	76.97909575944722	6.946277204604486	6.5135134107339105	9.561113625214386
9	71.31247599453911	10.631994858553169	12.197245979441819	5.858283167465897
10	32.36297397712969	37.48274458950186	13.517974148257728	16.636307285110718
11	25.353189256201492	27.5185293636699	29.20889409456155	17.919387285567062
12	26.29705545689285	24.48918280657588	32.50329896828808	16.7104627682432
13	23.898600933218233	33.18628998216466	24.325280174626656	18.589828909990455
14	14.656546027737955	34.986937226432815	31.2529234373158	19.10359330851343
15	13.527100976950953	30.506805191644386	34.64810371119672	21.317990120207938
16	14.517742174695107	29.950068641357465	32.059126638550964	23.473062545396466
17	13.327831883815472	28.22015431946182	29.224485760245816	29.22752803647689
18	19.712809123786418	25.99282783378524	27.85051775738608	26.443845285042265
19	20.02920585181833	32.45880567840859	27.566445214309347	19.94554325546374
20	21.805134601708996	29.668658089982923	25.72396667186389	22.80224063644419
21	18.325911446944605	32.71815972710782	25.190047193310033	23.76588163263754
22	16.571658915200352	31.438502287411442	25.403767098543128	26.586071698845075
23	18.455968755823108	31.840843318971256	22.65392967017923	27.04925825502641
24	14.631066964302692	31.676180117964257	28.522100235396124	25.170652682336925
25	18.108388696422665	29.546206471682112	26.6640300272664	25.681374804628827
26	21.181087689809516	31.6727575572043	23.73621943938455	23.409935313601636
27	16.850027190343816	29.47509326478071	27.053061100315258	26.62181844456022
28	21.496343564254776	29.13778088766015	29.246922547449998	20.118953000635077
29	20.606858051193903	31.151387468103636	25.855545118857933	22.386209361844532
30	23.58296477424409	28.057012256570367	26.572381455805232	21.78764151338031
31	25.883305889466502	34.06702895106118	18.934746977688707	21.114918181783608
32	25.36840063735687	28.97007541042208	22.21241933214431	23.449104620076742
33	25.6353603766338	28.845342084947955	23.464696285761004	22.05460125265724
34	20.471096474382133	31.300078718897478	25.029947406649654	23.19887740007073
35	23.948418206502105	27.283893809348154	26.241153631146823	22.526534353002916
36	24.335547856906537	29.78008145694609	28.147139689915996	17.73723099623138
37	20.122375561395035	33.00717596906005	23.704655823487133	23.16579264605778
38	21.568977909271716	32.88244264358593	25.657036594780212	19.891542852362136
39	21.903628294690087	29.581572932868372	25.307174828206463	23.207623944235074
40	25.40300652948536	29.02521666711033	21.760641311829513	23.8111354915748
41	20.10754446476854	25.392738847205482	27.707150489996618	26.792566198029366
42	26.27728066139085	25.06227159160484	23.29813166210959	25.362316084894722
43	21.7461904997319	25.215145972216412	27.540966150874084	25.4976973771776
44	22.499534151452117	25.369161206414642	26.332041633550222	25.799263008583022
45	24.1351379101844	28.935469518293587	25.54637379687482	21.38301877464719
46	19.98547313099661	27.665699476348205	30.3984241009123	21.95040329174288
47	21.614231768208974	26.940877164294324	25.332273607112842	26.11261746038386
48	21.156749479960908	23.953361905377605	32.51166522792353	22.378223386737957
49	22.618943493521854	22.06486893493712	29.488783507820553	25.827404063720476
50	20.16078429881237	23.37570970600203	27.54172671993185	28.921779275253744
51	20.16458714410122	23.557105426279943	30.881765737124518	25.396541692494324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3319.0
1	2558.0
2	1797.0
3	1187.5
4	578.0
5	692.5
6	807.0
7	744.5
8	682.0
9	650.5
10	619.0
11	637.0
12	655.0
13	672.5
14	690.0
15	665.5
16	641.0
17	669.0
18	697.0
19	725.0
20	753.0
21	781.5
22	810.0
23	802.5
24	795.0
25	1026.0
26	1202.0
27	1147.0
28	1834.0
29	2521.0
30	2706.5
31	2892.0
32	3611.0
33	4330.0
34	4842.5
35	5355.0
36	5849.0
37	6343.0
38	6912.0
39	7481.0
40	8877.0
41	10273.0
42	12799.5
43	15326.0
44	21810.5
45	28295.0
46	29818.5
47	31342.0
48	31954.0
49	32566.0
50	30957.0
51	29348.0
52	25512.0
53	21676.0
54	18811.5
55	15947.0
56	13010.0
57	10073.0
58	8784.0
59	7495.0
60	6467.0
61	5439.0
62	4663.5
63	3888.0
64	3153.0
65	2418.0
66	2023.5
67	1629.0
68	1273.0
69	917.0
70	720.0
71	523.0
72	571.0
73	619.0
74	526.5
75	395.5
76	357.0
77	241.0
78	125.0
79	81.0
80	37.0
81	37.0
82	37.0
83	26.0
84	15.0
85	11.0
86	7.0
87	4.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262961.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.707889093570486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26324340865504	31.076681893739856
2	9.230863198752486	7.330761842679699
3	3.2830047077962887	3.9108356049253437
4	1.6550879387693296	2.6288019325103487
5	1.064586115571597	2.1136233703835994
6	0.785940119735681	1.872481386519116
7	0.6301486887211705	1.7515311970939376
8	0.4863428268837784	1.54493176250837
9	0.4014575149971614	1.4346927433158927
>10	3.9696358581497115	32.23458758606264
>50	0.1777597943924332	4.4638850575843705
>100	0.040944671742077304	3.1735339891487713
>500	0.005991903181767411	1.434296193771224
>1k	0.004993252651472842	5.0293554397568245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	3016	1.1469381391156863	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2928	1.1134731005738494	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	2442	0.9286548195359768	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	2292	0.8716121402033001	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGT	2005	0.7624704804134453	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	834	0.3171572970896825	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	635	0.24148067584166474	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	556	0.21143819805978836	No Hit
AACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	551	0.20953677541536578	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.20269165389544458	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	508	0.19318454067333177	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	452	0.17188860705579914	TruSeq Adapter, Index 14 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	447	0.1699871844113766	No Hit
ACTTACGGGACTGTGACCGAAGGCAAGTCAGTTCTCGGCCTCGCATCTGTA	417	0.15857864854484124	No Hit
GAATGAGGATAGACAGAGTGAGGGAGTTTCAGGTAGAGAGAGGAAGAAATG	330	0.12549389453188875	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTAT	325	0.12359247188746621	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	287	0.10914165978985477	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACTC	282	0.1072402371454322	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.1068599526165477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4591517373298701	0.0
2	0.0	0.0	0.0	1.953521624879735	0.0
3	0.0	0.0	0.0	2.6498225972672755	0.0
4	0.0	0.0	0.0	5.851818330474861	0.0
5	0.0	0.0	0.0	6.399428052068558	0.0
6	0.0	0.0	0.0	8.700529736348736	0.0
7	0.0	0.0	0.0	10.520571491590008	0.0
8	0.0	0.0	0.0	12.282809998440833	0.0
9	0.0	0.0	0.0	14.851251706526824	0.0
10	0.0	0.0	0.0	17.712512501853887	0.0
11	0.0	0.0	0.0	20.836169622111264	0.0
12	0.0	0.0	0.0	22.262236605428182	0.0
13	0.0	0.0	0.0	22.977171519731062	0.0
14	0.0	0.0	0.0	23.647613144154455	0.0
15	0.0	0.0	0.0	24.100151733527024	0.0
16	0.0	0.0	0.0	24.989256962059013	0.0
17	0.0	0.0	0.0	26.063560756157756	0.0
18	0.0	0.0	0.0	27.86610942307034	0.0
19	0.0	0.0	0.0	28.410296583904078	0.0
20	0.0	0.0	0.0	29.081879061914123	0.0
21	0.0	0.0	0.0	29.661812968463003	0.0
22	0.0	0.0	0.0	30.282437319602526	0.0
23	0.0	0.0	0.0	30.872638908431288	0.0
24	0.0	0.0	0.0	31.295515304550864	0.0
25	0.0	0.0	0.0	31.635109388844732	0.0
26	0.0	0.0	0.0	31.989154285236214	0.0
27	0.0	0.0	0.0	32.44017173649324	0.0
28	0.0	0.0	0.0	32.810949152155644	0.0
29	0.0	0.0	0.0	33.19693794897342	0.0
30	0.0	0.0	0.0	33.88601351531216	0.0
31	0.0	0.0	0.0	34.22332589243272	0.0
32	0.0	0.0	0.0	34.608934404721616	0.0
33	0.0	0.0	0.0	34.952331334304326	0.0
34	0.0	0.0	0.0	35.30751708428246	0.0
35	0.0	0.0	0.0	35.696167872802434	0.0
36	0.0	0.0	0.0	36.03157882727857	0.0
37	0.0	0.0	0.0	36.300059704671035	0.0
38	0.0	0.0	0.0	36.61797757081849	0.0
39	0.0	0.0	0.0	36.92372633204163	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTG	30	2.159095E-6	45.000004	5
GCAAGTC	60	0.0	45.000004	23
CGTAGCG	30	2.159095E-6	45.000004	1
CTCGCAG	30	2.159095E-6	45.000004	1
GTAACGG	30	2.159095E-6	45.000004	2
TACAACG	30	2.159095E-6	45.000004	1
GCGAGAC	140	0.0	45.0	21
CCTTCAT	25	3.8825747E-5	45.0	32
AGCGTTG	25	3.8825747E-5	45.0	1
CGGGTAC	35	1.2071541E-7	45.0	6
CTCACGG	20	7.0233556E-4	45.0	2
GTACCGG	35	1.2071541E-7	45.0	2
ACCGGGT	35	1.2071541E-7	45.0	4
ATAGGCG	20	7.0233556E-4	45.0	1
TTTACGG	20	7.0233556E-4	45.0	2
TTAGCGG	45	3.8198777E-10	45.0	2
ACCGCGC	20	7.0233556E-4	45.0	35
GGCAATA	25	3.8825747E-5	45.0	8
ATTACGG	20	7.0233556E-4	45.0	2
GGCCGGT	20	7.0233556E-4	45.0	9
>>END_MODULE