SRR3548683_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494654
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	27777	5.615440287554533	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	24957	5.045344826889099	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGT	24029	4.857738944797778	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	7879	1.592830544178355	TruSeq Adapter, Index 13 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	6200	1.2534013674204596	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	5762	1.1648546256575303	No Hit
AACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	4875	0.9855373655120548	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT	4670	0.94409425578283	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGTC	3537	0.7150452639622847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3428	0.6930096592769895	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	2140	0.43262563327093284	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCTGACTGTCGTATGCCGT	1335	0.2698856170171473	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATG	1240	0.25068027348409194	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	1211	0.24481758966873815	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	1156	0.23369870657065345	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	1103	0.22298414649431722	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1032	0.2086306792222442	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTCTGCCGTCTTCT	850	0.17183728424312752	No Hit
AATCTGTCTCTTATACCCATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	836	0.16900702309088778	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	817	0.16516595438427667	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	754	0.15242977919919784	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	707	0.14292818818810724	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTGACTGT	656	0.1326179511335196	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	632	0.1277660748725372	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCCTGTCGTATGCC	622	0.1257444597637945	No Hit
AATGATACGGCTGTCTCTTATACACCTCTGACGCTCTGACTGTCGTATGCC	496	0.10027210939363676	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8875718E-5	45.000004	1
CGAACTG	30	2.162984E-6	45.000004	12
TACCCGG	25	3.8875718E-5	45.000004	2
CGAAACG	25	3.8875718E-5	45.000004	1
GCGAAGT	30	2.162984E-6	45.000004	43
ATAATCG	25	3.8875718E-5	45.000004	10
GACCGAA	30	2.162984E-6	45.000004	9
GGCCGAT	25	3.8875718E-5	45.000004	8
ACCGAAC	30	2.162984E-6	45.000004	10
ACGGGTC	30	2.162984E-6	45.000004	5
TCTAGCG	25	3.8875718E-5	45.000004	1
ACGGCTA	50	2.1827873E-11	45.000004	29
CGTAGCG	25	3.8875718E-5	45.000004	1
TCATCGA	30	2.162984E-6	45.000004	16
CGTAACG	25	3.8875718E-5	45.000004	1
TTTGCGC	25	3.8875718E-5	45.000004	13
AGCTACG	30	2.162984E-6	45.000004	1
GCTAACG	30	2.162984E-6	45.000004	1
TCGACAA	25	3.8875718E-5	45.000004	19
ATGCACG	25	3.8875718E-5	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content