##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494654
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.943000562009	31.0	31.0	33.0	28.0	34.0
2	31.222620255774743	31.0	31.0	34.0	30.0	34.0
3	31.323866783650793	31.0	31.0	34.0	30.0	34.0
4	35.24113420694061	35.0	35.0	37.0	33.0	37.0
5	34.35668366171102	35.0	35.0	37.0	30.0	37.0
6	34.55574805823869	35.0	35.0	37.0	32.0	37.0
7	34.2279290170503	35.0	35.0	37.0	30.0	37.0
8	34.76709376655197	35.0	35.0	37.0	32.0	37.0
9	36.38175775390475	37.0	35.0	39.0	32.0	39.0
10	35.81655864503269	37.0	35.0	39.0	30.0	39.0
11	36.04043634540507	37.0	35.0	39.0	32.0	39.0
12	35.53827119562361	37.0	35.0	39.0	30.0	39.0
13	35.50659450848472	37.0	35.0	39.0	30.0	39.0
14	36.34845568821843	38.0	35.0	40.0	30.0	41.0
15	36.56026434639162	38.0	35.0	40.0	31.0	41.0
16	36.09701528745345	38.0	34.0	40.0	30.0	41.0
17	36.09245048053791	38.0	34.0	40.0	30.0	41.0
18	35.72778750399269	38.0	34.0	40.0	29.0	41.0
19	35.63776296158527	38.0	34.0	40.0	27.0	41.0
20	35.944409627739795	38.0	34.0	40.0	30.0	41.0
21	36.355747653915664	38.0	35.0	40.0	30.0	41.0
22	36.219882988917504	38.0	34.0	40.0	30.0	41.0
23	36.171715987336604	38.0	34.0	40.0	30.0	41.0
24	35.64097530799306	38.0	34.0	40.0	29.0	41.0
25	35.86994343520926	38.0	34.0	40.0	30.0	41.0
26	35.67265603836217	38.0	34.0	40.0	29.0	41.0
27	35.63632357162785	38.0	34.0	40.0	29.0	41.0
28	35.684516854205164	38.0	34.0	40.0	29.0	41.0
29	35.29292192118127	38.0	34.0	40.0	27.0	41.0
30	35.580866625964816	38.0	34.0	40.0	28.0	41.0
31	35.325615480719854	38.0	34.0	40.0	27.0	41.0
32	35.00441925062771	37.0	34.0	40.0	26.0	41.0
33	35.331902703708046	38.0	34.0	40.0	27.0	41.0
34	35.330936371686064	38.0	34.0	40.0	27.0	41.0
35	35.287637419287016	38.0	34.0	40.0	27.0	41.0
36	35.52514484872254	38.0	34.0	40.0	29.0	41.0
37	35.15208812624582	38.0	34.0	40.0	27.0	41.0
38	35.19239104505372	38.0	34.0	40.0	27.0	41.0
39	34.80041402677427	37.0	34.0	40.0	25.0	41.0
40	34.51068019261949	37.0	33.0	40.0	25.0	41.0
41	34.81471493205352	37.0	33.0	40.0	26.0	41.0
42	34.90529137538562	37.0	33.0	40.0	26.0	41.0
43	35.074003242670635	37.0	34.0	40.0	27.0	41.0
44	35.01569581970428	37.0	34.0	40.0	27.0	41.0
45	34.5935583256175	37.0	33.0	40.0	25.0	41.0
46	34.47368261451439	37.0	33.0	40.0	24.0	40.0
47	34.41201122400708	37.0	33.0	40.0	24.0	40.0
48	34.62601737780347	37.0	33.0	40.0	26.0	40.0
49	34.45687086327008	37.0	33.0	40.0	24.0	40.0
50	34.36770550728388	37.0	33.0	40.0	24.0	40.0
51	32.76287465582003	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	5.0
12	10.0
13	12.0
14	14.0
15	32.0
16	74.0
17	135.0
18	372.0
19	788.0
20	1180.0
21	1896.0
22	2600.0
23	3202.0
24	3640.0
25	4123.0
26	4835.0
27	6149.0
28	7841.0
29	10751.0
30	14565.0
31	20219.0
32	27321.0
33	36209.0
34	49903.0
35	55699.0
36	60137.0
37	71737.0
38	74033.0
39	37165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.250769224548876	51.701189114006965	14.49639546026111	19.551646201183047
2	15.988751733534956	51.356301576455465	15.515289475067421	17.13965721494216
3	15.060830398622066	23.31730866423803	41.5520747835861	20.0697861535538
4	34.8043278736248	23.402620821826975	16.518819215047287	25.27423208950094
5	13.723532004188787	45.65514480829024	19.75400987356819	20.867313313952785
6	20.019852260367855	26.80742498797139	36.18691044649391	16.985812305166846
7	55.955880271866796	22.862647426281804	12.985238166475959	8.196234135375434
8	57.73894479777783	5.103365180510013	8.85871740651041	28.29897261520174
9	64.35164781847513	7.458142459173484	16.874623474185995	11.315586248165383
10	34.325407254363654	27.627998560610045	18.755736332871056	19.290857852155245
11	23.069054328884434	19.50130798497536	31.785854354761106	25.643783331379105
12	23.62418983774517	18.90735746602676	42.96639671366248	14.502055982565592
13	23.129298459124964	32.22676860997788	22.80604220323701	21.83789072766014
14	11.421316718352626	27.293219098602258	43.14995936553632	18.135504817508803
15	10.337933181577426	29.12763264827536	27.98663308090099	32.54780108924622
16	12.37551904967917	27.093281364347604	37.18296021057143	23.348239375401796
17	11.28081446829501	27.127446659685354	30.74209447411726	30.84964439790237
18	13.495291658411738	27.195979411871736	28.43745325015061	30.87127567956592
19	15.551678547024789	30.489594747035305	36.76529452910519	17.193432176834715
20	17.636165885649362	36.31346355230121	26.552903645780685	19.497466916268746
21	13.6919948084924	25.558471173790164	31.279641931531938	29.469892086185496
22	14.229946588928827	34.62966032822943	29.103171105459573	22.03722197738217
23	20.718926764971073	25.92397918545084	21.241514270581053	32.11557977899704
24	11.905291375385623	41.228818527698145	23.932283980317557	22.933606116598675
25	15.055574199339336	23.323575671075137	30.92545496448022	30.695395165105303
26	21.833241012910033	30.96042890586147	20.591160690098533	26.61516939112996
27	12.061562223291432	26.369340993906853	34.88964003121374	26.679456751587978
28	22.170244251537436	20.950401694922107	31.109219777864933	25.77013427567552
29	14.252588678146743	31.48887909528681	28.522765407739552	25.7357668188269
30	22.02024041046873	20.20078681260032	30.28682675162841	27.49214602530253
31	28.98167203742414	29.121365641438256	21.426087730009257	20.470874591128343
32	18.730466144011775	26.467591488191744	21.49522696672826	33.30671540106822
33	24.54725929639708	20.880251650648738	29.21334912888605	25.359139924068135
34	16.11833726200536	22.137494086775806	29.648400700287475	32.09576795093136
35	22.111213090362153	19.9377746869529	35.47934515843398	22.471667064250973
36	22.72457111435468	18.137930755639296	33.18764227116328	25.949855858842746
37	19.75542500414431	26.80095581962341	28.65477687434045	24.78884230189183
38	30.50839576754661	21.662414536221277	25.280094773316296	22.549094922915817
39	16.780214048607714	25.910838687244013	34.47015489614963	22.838792367998643
40	25.523901555430665	23.617720669397194	22.991828631730463	27.866549143441677
41	18.425404424102503	16.961350762351056	42.91706930500916	21.69617550853728
42	31.25396741965091	20.07079696110817	23.98120706594913	24.694028553291798
43	21.520294994076668	18.716921322783197	31.150258564572407	28.612525118567728
44	18.79657295806766	16.938910834644012	31.14843911097454	33.11607709631379
45	33.30631107804648	24.238558669292072	23.823318925956325	18.63181132670513
46	15.617987522591548	19.949904377605357	43.62665620817784	20.805451891625257
47	22.377661961694436	24.36915500531685	23.300327097324594	29.952855935664118
48	18.239011511076427	16.201425642974684	42.737347721841935	22.82221512410695
49	25.409680301786704	15.929518410848795	31.79555810728307	26.865243180081432
50	20.78280980240734	17.534074322657858	26.248852733425785	35.434263141509014
51	15.925677342142183	16.688837045692544	40.26632757442576	27.11915803773951
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3920.0
1	3065.5
2	2211.0
3	1479.5
4	748.0
5	954.5
6	1161.0
7	1057.0
8	953.0
9	967.0
10	981.0
11	1024.0
12	1067.0
13	1046.5
14	1026.0
15	1025.5
16	1025.0
17	1026.0
18	1027.0
19	1081.0
20	1135.0
21	1257.0
22	1379.0
23	1419.0
24	1459.0
25	2274.5
26	2779.5
27	2469.0
28	3039.5
29	3610.0
30	3962.0
31	4314.0
32	5236.0
33	6158.0
34	6927.0
35	7696.0
36	8986.0
37	10276.0
38	11273.0
39	12270.0
40	13714.5
41	15159.0
42	19987.5
43	24816.0
44	62250.0
45	99684.0
46	93823.0
47	87962.0
48	74020.0
49	60078.0
50	50352.0
51	40626.0
52	34657.5
53	28689.0
54	24778.5
55	20868.0
56	17011.0
57	13154.0
58	11657.5
59	10161.0
60	9080.5
61	8000.0
62	7056.0
63	6112.0
64	5021.5
65	3931.0
66	3267.5
67	2604.0
68	2172.0
69	1740.0
70	1439.0
71	1138.0
72	957.5
73	777.0
74	599.5
75	383.0
76	344.0
77	240.5
78	137.0
79	118.0
80	99.0
81	86.0
82	73.0
83	74.5
84	76.0
85	45.0
86	14.0
87	9.5
88	5.0
89	5.0
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.984420876950775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5825575535326	23.562524792723284
2	9.270097265650724	5.559169959670837
3	3.2527885244299295	2.92598940420668
4	1.6178588060603325	1.9404223744157636
5	1.0185297177104173	1.5270011865755506
6	0.7168804616716791	1.289714728873584
7	0.5656227526135008	1.1871909470359832
8	0.438578998590987	1.0520429825235003
9	0.36671495395500825	0.9896161969132731
>10	3.828085940569432	24.66024813544083
>50	0.257728447840047	4.9565664924961945
>100	0.0676452619002748	4.345641326747594
>500	0.005411620952021984	1.191425771449959
>1k	0.007440978809030228	5.2182177089024675
>5k	0.0020293578570082437	4.024357972648729
>10k+	0.0020293578570082437	15.569870019375756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	27777	5.615440287554533	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	24957	5.045344826889099	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGT	24029	4.857738944797778	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	7879	1.592830544178355	TruSeq Adapter, Index 13 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	6200	1.2534013674204596	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	5762	1.1648546256575303	No Hit
AACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	4875	0.9855373655120548	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT	4670	0.94409425578283	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGTC	3537	0.7150452639622847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3428	0.6930096592769895	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	2140	0.43262563327093284	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCTGACTGTCGTATGCCGT	1335	0.2698856170171473	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATG	1240	0.25068027348409194	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	1211	0.24481758966873815	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	1156	0.23369870657065345	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	1103	0.22298414649431722	No Hit
AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1032	0.2086306792222442	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTCTGCCGTCTTCT	850	0.17183728424312752	No Hit
AATCTGTCTCTTATACCCATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	836	0.16900702309088778	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	817	0.16516595438427667	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	754	0.15242977919919784	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	707	0.14292818818810724	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTGACTGT	656	0.1326179511335196	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	632	0.1277660748725372	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCCTGTCGTATGCC	622	0.1257444597637945	No Hit
AATGATACGGCTGTCTCTTATACACCTCTGACGCTCTGACTGTCGTATGCC	496	0.10027210939363676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0216151087426766E-4	0.0	0.0	1.9225559684142854	0.0
2	2.0216151087426766E-4	0.0	0.0	2.3497232408916133	0.0
3	2.0216151087426766E-4	0.0	0.0	4.027057296615412	0.0
4	2.0216151087426766E-4	0.0	0.0	11.706566610196218	0.0
5	2.0216151087426766E-4	0.0	0.0	12.564742223857484	0.0
6	2.0216151087426766E-4	0.0	0.0	15.463940451305357	0.0
7	2.0216151087426766E-4	0.0	0.0	17.934556275699784	0.0
8	2.0216151087426766E-4	0.0	0.0	19.036942994497164	0.0
9	2.0216151087426766E-4	0.0	0.0	25.641357393248615	0.0
10	2.0216151087426766E-4	0.0	0.0	27.524896190064165	0.0
11	2.0216151087426766E-4	0.0	0.0	35.59215128150182	0.0
12	2.0216151087426766E-4	0.0	0.0	36.84272238777004	0.0
13	2.0216151087426766E-4	0.0	0.0	37.68735318020273	0.0
14	2.0216151087426766E-4	0.0	0.0	39.21973743262968	0.0
15	2.0216151087426766E-4	0.0	0.0	39.81712469726314	0.0
16	2.0216151087426766E-4	0.0	0.0	40.55885528066082	0.0
17	2.0216151087426766E-4	0.0	0.0	41.3250474068743	0.0
18	2.0216151087426766E-4	0.0	0.0	43.61230274090576	0.0
19	2.0216151087426766E-4	0.0	0.0	44.19149546956054	0.0
20	2.0216151087426766E-4	0.0	0.0	44.71853052840976	0.0
21	2.0216151087426766E-4	0.0	0.0	45.28377411281421	0.0
22	2.0216151087426766E-4	0.0	0.0	45.73621157415082	0.0
23	2.0216151087426766E-4	0.0	0.0	46.211695447727095	0.0
24	2.0216151087426766E-4	0.0	0.0	46.516150683103746	0.0
25	2.0216151087426766E-4	0.0	0.0	46.7710763483162	0.0
26	2.0216151087426766E-4	0.0	0.0	47.022969590865536	0.0
27	2.0216151087426766E-4	0.0	0.0	47.325201049622564	0.0
28	2.0216151087426766E-4	0.0	0.0	47.58861749829173	0.0
29	2.0216151087426766E-4	0.0	0.0	47.86962199840697	0.0
30	2.0216151087426766E-4	0.0	0.0	48.37563226012526	0.0
31	2.0216151087426766E-4	0.0	0.0	48.64996543038164	0.0
32	2.0216151087426766E-4	0.0	0.0	48.902262995952725	0.0
33	2.0216151087426766E-4	0.0	0.0	49.154762723034686	0.0
34	2.0216151087426766E-4	0.0	0.0	49.3856311684531	0.0
35	2.0216151087426766E-4	0.0	0.0	49.65491030093762	0.0
36	2.0216151087426766E-4	0.0	0.0	49.88860900750828	0.0
37	2.0216151087426766E-4	0.0	0.0	50.13969360401412	0.0
38	2.0216151087426766E-4	0.0	0.0	50.372583664541274	0.0
39	2.0216151087426766E-4	0.0	0.0	50.61396450852515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8875718E-5	45.000004	1
CGAACTG	30	2.162984E-6	45.000004	12
TACCCGG	25	3.8875718E-5	45.000004	2
CGAAACG	25	3.8875718E-5	45.000004	1
GCGAAGT	30	2.162984E-6	45.000004	43
ATAATCG	25	3.8875718E-5	45.000004	10
GACCGAA	30	2.162984E-6	45.000004	9
GGCCGAT	25	3.8875718E-5	45.000004	8
ACCGAAC	30	2.162984E-6	45.000004	10
ACGGGTC	30	2.162984E-6	45.000004	5
TCTAGCG	25	3.8875718E-5	45.000004	1
ACGGCTA	50	2.1827873E-11	45.000004	29
CGTAGCG	25	3.8875718E-5	45.000004	1
TCATCGA	30	2.162984E-6	45.000004	16
CGTAACG	25	3.8875718E-5	45.000004	1
TTTGCGC	25	3.8875718E-5	45.000004	13
AGCTACG	30	2.162984E-6	45.000004	1
GCTAACG	30	2.162984E-6	45.000004	1
TCGACAA	25	3.8875718E-5	45.000004	19
ATGCACG	25	3.8875718E-5	45.000004	1
>>END_MODULE