FastQCFastQC Report
Sat 18 Jun 2016
SRR3548682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410101
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC78931.9246478306563504No Hit
AATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT76861.8741724599549865TruSeq Adapter, Index 11 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGT70301.7142118648820655No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT54541.3299162889141944TruSeq Adapter, Index 11 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43281.0553497796884181No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT19980.48719705633490284TruSeq Adapter, Index 11 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT19380.4725665141026235TruSeq Adapter, Index 11 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG16420.4003891724233787RNA PCR Primer, Index 11 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT14590.35576601861492657No Hit
AAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT14290.3484507474987869Illumina PCR Primer Index 11 (100% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT11390.2777364600427699TruSeq Adapter, Index 11 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCTC11320.27602956344900403No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9810.23920936549776764No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC9060.22092118770741842TruSeq Adapter, Index 11 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7610.18556404397940995No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC6840.16678818144798477RNA PCR Primer, Index 11 (96% over 26bp)
AAAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT5950.14508621047010373No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.12728571742083047No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.10924138200101925No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC4390.10704680066617736Illumina PCR Primer Index 11 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCACG302.1620745E-645.0000041
CGTTCAT302.1620745E-645.00000417
TATCTAG302.1620745E-645.0000041
TCTCGAG302.1620745E-645.0000041
GCCTACG302.1620745E-645.0000041
CGGGTTC351.2093733E-745.06
CTTCGCG253.8864033E-545.01
CACGGTC207.0279744E-445.012
TATGGGT406.7957444E-945.04
GACAACG351.2093733E-745.01
TTCACGG351.2093733E-745.02
TCGGGAT207.0279744E-445.05
TATTGCC207.0279744E-445.027
TCTACGG453.8380676E-1045.02
TATCTGG253.8864033E-545.02
ATGAGTA207.0279744E-445.027
CTAGTCA207.0279744E-445.037
ATATACG253.8864033E-545.01
CTACGCG207.0279744E-445.01
TGCGAAA207.0279744E-445.037
Produced by FastQC (version 0.11.5)