##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410101
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91973928373742	31.0	31.0	33.0	28.0	34.0
2	31.200245793109502	31.0	31.0	34.0	28.0	34.0
3	31.372920329382275	31.0	31.0	34.0	30.0	34.0
4	35.33729008219926	37.0	35.0	37.0	33.0	37.0
5	34.588140482466514	35.0	35.0	37.0	32.0	37.0
6	34.717196495497454	35.0	35.0	37.0	32.0	37.0
7	34.58932067953992	35.0	35.0	37.0	32.0	37.0
8	35.055266873282434	35.0	35.0	37.0	32.0	37.0
9	36.50469762326841	38.0	35.0	39.0	32.0	39.0
10	35.966442412966565	37.0	35.0	39.0	31.0	39.0
11	36.185978576009326	37.0	35.0	39.0	32.0	39.0
12	35.898161672368516	37.0	35.0	39.0	31.0	39.0
13	35.618362305870995	37.0	35.0	39.0	30.0	39.0
14	36.5565872797189	38.0	35.0	40.0	31.0	41.0
15	36.65827930192806	38.0	35.0	40.0	31.0	41.0
16	36.438577326073336	38.0	35.0	40.0	31.0	41.0
17	36.37370550181541	38.0	35.0	40.0	31.0	41.0
18	36.23637835557582	38.0	35.0	40.0	30.0	41.0
19	36.18482520159668	38.0	34.0	40.0	30.0	41.0
20	36.23417158212245	38.0	34.0	40.0	30.0	41.0
21	36.40680466519223	38.0	35.0	40.0	31.0	41.0
22	36.30620993365049	38.0	34.0	40.0	30.0	41.0
23	36.26790717408638	38.0	35.0	40.0	30.0	41.0
24	35.81640376395083	38.0	34.0	40.0	29.0	41.0
25	36.0368957890861	38.0	34.0	40.0	30.0	41.0
26	35.97056578745236	38.0	34.0	40.0	30.0	41.0
27	35.80557960112265	38.0	34.0	40.0	29.0	41.0
28	35.74528713658343	38.0	34.0	40.0	29.0	41.0
29	35.237887739849455	38.0	34.0	40.0	27.0	41.0
30	35.1982511625185	37.0	34.0	40.0	27.0	41.0
31	35.075454583139276	37.0	34.0	40.0	27.0	41.0
32	35.09452549493905	37.0	34.0	40.0	27.0	41.0
33	34.91178026876306	37.0	33.0	40.0	25.0	41.0
34	35.08530581490901	37.0	34.0	40.0	27.0	41.0
35	34.874523592968565	37.0	33.0	40.0	25.0	41.0
36	34.92482827401055	37.0	33.0	40.0	26.0	41.0
37	34.69757449994026	37.0	33.0	40.0	25.0	41.0
38	34.561003265049344	37.0	33.0	40.0	25.0	41.0
39	34.645853094725446	37.0	33.0	40.0	25.0	41.0
40	34.329228653429276	37.0	33.0	40.0	24.0	41.0
41	34.605419152842835	37.0	33.0	40.0	25.0	41.0
42	34.610654448538284	37.0	33.0	40.0	25.0	41.0
43	34.5999205073872	37.0	33.0	40.0	25.0	41.0
44	34.43163025693671	37.0	33.0	40.0	24.0	41.0
45	34.17562990580369	36.0	33.0	40.0	24.0	41.0
46	34.10146281038086	36.0	33.0	39.0	24.0	41.0
47	34.00994876871795	36.0	33.0	39.0	24.0	40.0
48	34.11949739210585	36.0	33.0	39.0	24.0	40.0
49	34.0027651724819	36.0	33.0	39.0	24.0	40.0
50	33.93134130372762	36.0	33.0	39.0	24.0	40.0
51	32.54458779666472	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	10.0
13	12.0
14	21.0
15	50.0
16	92.0
17	198.0
18	383.0
19	680.0
20	1300.0
21	1823.0
22	2462.0
23	3038.0
24	3464.0
25	3893.0
26	4416.0
27	5439.0
28	6953.0
29	8798.0
30	11832.0
31	15849.0
32	21069.0
33	28061.0
34	41184.0
35	45122.0
36	50106.0
37	61123.0
38	62156.0
39	30558.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.355475846193986	40.02379901536451	16.51934523446663	24.101379903974873
2	20.87461381464566	39.26325466165652	18.25867286351411	21.603458660183712
3	19.61224186237049	29.31497362844763	27.913367682595265	23.15941682658662
4	25.156729683663293	31.15915347682644	18.316707347702152	25.367409491808118
5	15.993621083586726	40.04769556767723	18.808293566706737	25.150389782029304
6	19.55664580188783	33.95480625504449	26.152094240199364	20.33645370286832
7	68.31804848074012	14.333054540223017	10.022409113852442	7.326487865184431
8	69.85230467616515	7.539849939405172	7.966330245476115	14.641515138953576
9	68.2044179360694	9.781980536501985	14.088968327314491	7.924633200114119
10	34.721690510386466	29.407146044510984	16.380111240889438	19.49105220421311
11	27.36789229970178	26.176722319623703	25.656606543266168	20.798778837408346
12	25.879966154678968	22.75951533890432	33.86287768135167	17.497640825065044
13	23.88606709078983	31.49638747528048	24.028958719925093	20.5885867140046
14	15.624931419333285	31.76144413205527	33.226936778988595	19.386687669622848
15	13.4522958978398	29.55369530920432	31.654641173759636	25.339367619196246
16	14.681017602980727	28.933848003296745	31.95578650137405	24.42934789234847
17	14.823177705004376	27.544678018341823	28.642212528133314	28.989931748520487
18	19.023606379891785	27.162333181338255	26.493961243693626	27.320099195076335
19	19.895099012194557	31.086000765665045	29.212559832821672	19.80634038931873
20	21.77804979748891	31.00845889183396	24.792429182079537	22.421062128597587
21	18.57152262491435	29.42762880363618	27.030170616506666	24.970677954942808
22	16.73173193920522	32.410308680056865	25.484209987295813	25.373749393442104
23	20.431552227378134	29.047478547967454	21.707091667662358	28.813877556992058
24	15.413276241706312	33.47126683426765	25.560532649274204	25.554924274751826
25	18.842187656211518	27.646116444485624	26.212810990463325	27.298884908839533
26	23.32327889958815	30.357155920126992	22.123086751800166	24.196478428484692
27	18.049943794333593	28.016513005332833	27.281815942901872	26.651727257431705
28	24.832419330847767	27.43446126685865	25.5656533390555	22.16746606323808
29	22.498847844799208	30.21962882314357	24.873872533839226	22.407650798218
30	23.322791214847076	27.132584412132623	26.07772231718528	23.466902055835025
31	25.74755974747684	33.030887513076046	19.468131021382536	21.753421718064576
32	22.92069514582993	25.465190282393852	22.057493154125446	29.556621417650774
33	24.803645931124287	29.922872658198834	23.358148358575082	21.915333052101797
34	21.426185256802594	25.479333139885053	26.49981346058654	26.594668142725812
35	23.0501754445856	25.607594226788034	25.715616396936365	25.626613931690002
36	24.331567101762737	27.15331101362835	29.3276534317156	19.18746845289331
37	19.131384707669575	27.833143542688266	25.056510469372178	27.97896128026998
38	21.41691924672215	31.09746135708033	25.12551786023443	22.360101535963093
39	19.75025664409499	23.86509664692356	28.353990846157412	28.030655862824037
40	22.180145866506056	27.36789229970178	24.341808481325334	26.11015335246683
41	19.162108846357363	21.242571951787486	33.06258702124599	26.532732180609166
42	26.12892921499826	22.757564599940014	23.81218285251682	27.301323332544907
43	22.276951287609638	23.163805989256307	28.540286417248435	26.01895630588562
44	21.777562112747837	21.58565816713444	27.710734672678193	28.926045047439537
45	25.91629866788913	26.456653361001315	25.280357765526052	22.346690205583503
46	18.560305875869602	22.9121606628611	33.67341215944365	24.85412130182565
47	21.31596850531942	26.799739576348262	24.6805055340026	27.203786384329714
48	20.707825633197675	20.682953711402803	35.26936047461479	23.339860180784733
49	22.639057207858553	20.015800985610863	29.812168221974588	27.532973584555997
50	19.954840392976365	21.11138475643805	27.53589969300246	31.397875157583133
51	19.18527387155847	20.608094103647637	33.35129638796296	26.85533563683093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4965.0
1	3886.5
2	2808.0
3	1882.5
4	957.0
5	1148.0
6	1339.0
7	1166.5
8	994.0
9	1015.5
10	1037.0
11	1038.0
12	1039.0
13	1054.0
14	1069.0
15	1140.5
16	1212.0
17	1169.0
18	1126.0
19	1137.0
20	1148.0
21	1207.5
22	1267.0
23	1487.0
24	1707.0
25	1787.5
26	2169.5
27	2471.0
28	2877.5
29	3284.0
30	3812.5
31	4341.0
32	5069.5
33	5798.0
34	6476.5
35	7155.0
36	7157.0
37	7159.0
38	8531.5
39	9904.0
40	10880.5
41	11857.0
42	16103.0
43	20349.0
44	34589.5
45	48830.0
46	49470.5
47	50111.0
48	49512.0
49	48913.0
50	43959.5
51	39006.0
52	34269.5
53	29533.0
54	25684.0
55	21835.0
56	19153.0
57	16471.0
58	14767.5
59	13064.0
60	12275.0
61	11486.0
62	10568.5
63	9651.0
64	8363.5
65	7076.0
66	6271.0
67	5466.0
68	4746.0
69	4026.0
70	3598.0
71	3170.0
72	2692.0
73	2214.0
74	2126.0
75	1491.5
76	945.0
77	816.0
78	687.0
79	528.5
80	370.0
81	321.0
82	272.0
83	159.0
84	46.0
85	34.5
86	23.0
87	15.0
88	7.0
89	6.0
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.59823039064054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34042391220751	29.03719113630056
2	8.83723052631594	6.468539976346246
3	3.1205368631747383	3.4261838118286745
4	1.5739100170012312	2.3040928566539214
5	0.969332995159358	1.7737936141045918
6	0.6576673054535119	1.4441675739227637
7	0.5051892917582332	1.2942323863456642
8	0.4108861177850035	1.2030163842409138
9	0.3437327909272731	1.1322010687656794
>10	3.973135946623064	31.73179706711487
>50	0.20297533194754241	4.668210935276456
>100	0.052920961134837796	3.85229759077774
>500	0.004019313503911731	1.0907447324743949
>1k	0.005359084671882308	3.693429848219096
>5k	0.002679542335941154	6.8801010176284425
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC	7893	1.9246478306563504	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	7686	1.8741724599549865	TruSeq Adapter, Index 11 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGT	7030	1.7142118648820655	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	5454	1.3299162889141944	TruSeq Adapter, Index 11 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4328	1.0553497796884181	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	1998	0.48719705633490284	TruSeq Adapter, Index 11 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	1938	0.4725665141026235	TruSeq Adapter, Index 11 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	1642	0.4003891724233787	RNA PCR Primer, Index 11 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT	1459	0.35576601861492657	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	1429	0.3484507474987869	Illumina PCR Primer Index 11 (100% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	1139	0.2777364600427699	TruSeq Adapter, Index 11 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCTC	1132	0.27602956344900403	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	981	0.23920936549776764	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	906	0.22092118770741842	TruSeq Adapter, Index 11 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	761	0.18556404397940995	No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	684	0.16678818144798477	RNA PCR Primer, Index 11 (96% over 26bp)
AAAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	595	0.14508621047010373	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.12728571742083047	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	448	0.10924138200101925	No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	439	0.10704680066617736	Illumina PCR Primer Index 11 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001219211852689947	0.0	0.0	1.571076393376266	0.0
2	0.001219211852689947	0.0	0.0	2.053152759929871	0.0
3	0.001219211852689947	0.0	0.0	2.970975442634863	0.0
4	0.001219211852689947	0.0	0.0	7.127024806084354	0.0
5	0.001219211852689947	0.0	0.0	7.80076127588082	0.0
6	0.001219211852689947	0.0	0.0	10.277468233435178	0.0
7	0.001219211852689947	0.0	0.0	12.322818037507833	0.0
8	0.001219211852689947	0.0	0.0	13.673948612658833	0.0
9	0.001219211852689947	0.0	0.0	16.967771353885993	0.0
10	0.001219211852689947	0.0	0.0	19.338894564997403	0.0
11	0.001219211852689947	0.0	0.0	23.45787988812512	0.0
12	0.001219211852689947	0.0	0.0	24.720495682770828	0.0
13	0.001219211852689947	0.0	0.0	25.401303581312895	0.0
14	0.0014630542232279364	0.0	0.0	26.301325770968614	0.0
15	0.0014630542232279364	0.0	0.0	26.821929232067223	0.0
16	0.0014630542232279364	0.0	0.0	27.642458808927557	0.0
17	0.0014630542232279364	0.0	0.0	28.56491449667277	0.0
18	0.0014630542232279364	0.0	0.0	30.503461342449786	0.0
19	0.0014630542232279364	0.0	0.0	31.068687957356847	0.0
20	0.0014630542232279364	0.0	0.0	31.710724918983374	0.0
21	0.0014630542232279364	0.0	0.0	32.3091140962836	0.0
22	0.0014630542232279364	0.0	0.0	32.848005735172556	0.0
23	0.0014630542232279364	0.0	0.0	33.41225698059746	0.0
24	0.0014630542232279364	0.0	0.0	33.78509196515005	0.0
25	0.0014630542232279364	0.0	0.0	34.107939263742345	0.0
26	0.0014630542232279364	0.0	0.0	34.431761931816794	0.0
27	0.0014630542232279364	0.0	0.0	34.81459445356144	0.0
28	0.0014630542232279364	0.0	0.0	35.14573239275203	0.0
29	0.0014630542232279364	0.0	0.0	35.46736047949164	0.0
30	0.001706896593765926	0.0	0.0	36.06501812968025	0.0
31	0.001706896593765926	0.0	0.0	36.39152306383062	0.0
32	0.001706896593765926	0.0	0.0	36.734853121548106	0.0
33	0.001706896593765926	0.0	0.0	37.055018154064484	0.0
34	0.001706896593765926	0.0	0.0	37.37908466450948	0.0
35	0.001706896593765926	0.0	0.0	37.74240979661108	0.0
36	0.001706896593765926	0.0	0.0	38.031850690439676	0.0
37	0.001706896593765926	0.0	0.0	38.32446153508526	0.0
38	0.001706896593765926	0.0	0.0	38.601222625645875	0.0
39	0.001706896593765926	0.0	0.0	38.91309701756396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCACG	30	2.1620745E-6	45.000004	1
CGTTCAT	30	2.1620745E-6	45.000004	17
TATCTAG	30	2.1620745E-6	45.000004	1
TCTCGAG	30	2.1620745E-6	45.000004	1
GCCTACG	30	2.1620745E-6	45.000004	1
CGGGTTC	35	1.2093733E-7	45.0	6
CTTCGCG	25	3.8864033E-5	45.0	1
CACGGTC	20	7.0279744E-4	45.0	12
TATGGGT	40	6.7957444E-9	45.0	4
GACAACG	35	1.2093733E-7	45.0	1
TTCACGG	35	1.2093733E-7	45.0	2
TCGGGAT	20	7.0279744E-4	45.0	5
TATTGCC	20	7.0279744E-4	45.0	27
TCTACGG	45	3.8380676E-10	45.0	2
TATCTGG	25	3.8864033E-5	45.0	2
ATGAGTA	20	7.0279744E-4	45.0	27
CTAGTCA	20	7.0279744E-4	45.0	37
ATATACG	25	3.8864033E-5	45.0	1
CTACGCG	20	7.0279744E-4	45.0	1
TGCGAAA	20	7.0279744E-4	45.0	37
>>END_MODULE