FastQCFastQC Report
Sat 18 Jun 2016
SRR3548681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417577
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC84422.02166307052352No Hit
AATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT82501.9756835266310164TruSeq Adapter, Index 11 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGT74011.772367730981352No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT54381.3022747900387235TruSeq Adapter, Index 11 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42931.0280738642214489No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT21250.5088881811019285TruSeq Adapter, Index 11 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT20830.4988301558754433TruSeq Adapter, Index 11 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG17840.4272265953345132RNA PCR Primer, Index 11 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT15630.3743022244999126No Hit
AAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT13660.32712529665187495Illumina PCR Primer Index 11 (100% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT11380.2725245882795269TruSeq Adapter, Index 11 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCTC11290.27036929715956576No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10300.2466610948399936No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC8880.21265539050282942TruSeq Adapter, Index 11 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.19206038646764548No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC7100.17002852168582083RNA PCR Primer, Index 11 (96% over 26bp)
AAAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT5950.14248869070853998No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.12380950100221036No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.1137514757757252No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC4280.10249606659370608Illumina PCR Primer Index 11 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.0281216E-445.012
CTTCGTG207.0281216E-445.013
CTTCGCA207.0281216E-445.035
TGTCACG207.0281216E-445.01
TGCCCTT207.0281216E-445.030
TACCCAA207.0281216E-445.030
CGAACAG207.0281216E-445.01
ACGCCTC207.0281216E-445.045
AATTTGG253.8865244E-545.02
TTTCGCG207.0281216E-445.013
CGTGACA207.0281216E-445.036
CGGTTAG207.0281216E-445.01
TAAACGG551.8189894E-1245.02
CACGCCG551.8189894E-1245.026
TAAGTTA253.8865244E-545.018
TAACGCG207.0281216E-445.01
CGTTCCG207.0281216E-445.043
TAGCCGT351.209446E-745.044
TGTGATC207.0281216E-445.037
CGTTAAG207.0281216E-445.01
Produced by FastQC (version 0.11.5)