##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417577
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00168112707357	31.0	31.0	33.0	28.0	34.0
2	31.26771110477828	31.0	31.0	34.0	30.0	34.0
3	31.44814249826978	31.0	31.0	34.0	30.0	34.0
4	35.38525828769305	37.0	35.0	37.0	33.0	37.0
5	34.60958577699442	35.0	35.0	37.0	32.0	37.0
6	34.74704545508972	35.0	35.0	37.0	32.0	37.0
7	34.62726155894602	35.0	35.0	37.0	32.0	37.0
8	35.054284599008085	35.0	35.0	37.0	32.0	37.0
9	36.59793523110708	38.0	35.0	39.0	32.0	39.0
10	36.023155010932115	37.0	35.0	39.0	31.0	39.0
11	36.20001101593239	37.0	35.0	39.0	32.0	39.0
12	35.88268750434052	37.0	35.0	39.0	31.0	39.0
13	35.67712302162236	37.0	35.0	39.0	30.0	39.0
14	36.59802144275188	38.0	35.0	40.0	31.0	41.0
15	36.68926688969938	38.0	35.0	40.0	31.0	41.0
16	36.478857791497134	38.0	35.0	40.0	31.0	41.0
17	36.42725054301362	38.0	35.0	40.0	31.0	41.0
18	36.22622175071903	38.0	35.0	40.0	30.0	41.0
19	36.2581511912773	38.0	34.0	40.0	30.0	41.0
20	36.256379063023104	38.0	34.0	40.0	30.0	41.0
21	36.41821029414934	38.0	35.0	40.0	31.0	41.0
22	36.340492891131454	38.0	35.0	40.0	30.0	41.0
23	36.25547384075272	38.0	35.0	40.0	30.0	41.0
24	35.838868041103794	38.0	34.0	40.0	29.0	41.0
25	36.01169365170974	38.0	34.0	40.0	30.0	41.0
26	35.893938123986715	38.0	34.0	40.0	30.0	41.0
27	35.69005237357422	38.0	34.0	40.0	29.0	41.0
28	35.5873383831006	38.0	34.0	40.0	28.0	41.0
29	35.19745340380337	37.0	34.0	40.0	27.0	41.0
30	35.16152470083362	37.0	34.0	40.0	27.0	41.0
31	35.03711890262155	37.0	33.0	40.0	26.0	41.0
32	35.05450970719173	37.0	34.0	40.0	27.0	41.0
33	34.84528601910546	37.0	33.0	40.0	25.0	41.0
34	35.02276945329843	37.0	34.0	40.0	26.0	41.0
35	34.80573403228626	37.0	33.0	40.0	25.0	41.0
36	34.82704267715894	37.0	33.0	40.0	25.0	41.0
37	34.59315048482076	37.0	33.0	40.0	25.0	41.0
38	34.505803720032475	37.0	33.0	40.0	25.0	41.0
39	34.632280992487615	37.0	33.0	40.0	25.0	41.0
40	34.26010771666064	37.0	33.0	40.0	24.0	41.0
41	34.54115049439984	37.0	33.0	40.0	25.0	41.0
42	34.564422848959595	37.0	33.0	40.0	25.0	41.0
43	34.57582433898419	37.0	33.0	40.0	25.0	41.0
44	34.352038067230716	37.0	33.0	40.0	24.0	41.0
45	34.083924641443375	36.0	33.0	40.0	23.0	41.0
46	33.989106200772554	36.0	33.0	39.0	23.0	40.0
47	33.877371119577944	36.0	33.0	39.0	23.0	40.0
48	33.995117068229334	36.0	33.0	39.0	23.0	40.0
49	33.856862327187564	36.0	33.0	39.0	24.0	40.0
50	33.78478939213606	36.0	32.0	39.0	23.0	40.0
51	32.304973693474494	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	4.0
12	8.0
13	15.0
14	15.0
15	60.0
16	98.0
17	212.0
18	409.0
19	745.0
20	1283.0
21	1939.0
22	2546.0
23	3079.0
24	3570.0
25	3894.0
26	4716.0
27	5675.0
28	7128.0
29	9139.0
30	12177.0
31	16120.0
32	21657.0
33	28643.0
34	41893.0
35	46182.0
36	50721.0
37	62097.0
38	62903.0
39	30641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.144253634658998	40.37985808605359	16.495400848226794	23.980487431060617
2	20.756171915598802	39.54264722434425	18.150424951565817	21.55075590849113
3	19.392112113454523	29.251850556903275	28.211084422753167	23.14495290688903
4	25.322036414840852	30.939443503832827	18.43659971693843	25.301920364387886
5	15.903653697401914	40.164328974057476	18.93207719773838	24.999940130802223
6	19.58034087126446	33.9151821101258	26.282577823970186	20.22189919463955
7	67.92998656535202	14.546538722199736	10.148547453523541	7.374927258924702
8	69.55052601077166	7.526516067695299	8.083778560600798	14.839179360932237
9	67.98602413447101	9.8472856503112	14.226837206072174	7.939853009145619
10	34.79765408535432	29.33542795699955	16.423797287685865	19.44312066996027
11	27.241921849143992	26.165713149910076	25.75620783711747	20.836157163828467
12	25.71190463076271	22.907152453319988	34.03060992343927	17.350332992478034
13	23.76687413339336	31.606625843856346	23.8212353649746	20.80526465777569
14	15.403626157570937	31.783359715693155	33.59236739571384	19.22064673102206
15	13.429379491686563	29.680274536193323	31.570225371608114	25.320120600512002
16	14.704353927539113	28.861742864190315	32.08510047248771	24.34880273578286
17	14.726625269112045	27.748415262334852	28.74224394542803	28.782715523125074
18	18.91339800803205	27.337233612004493	26.461227510135853	27.288140869827597
19	19.72594276025739	31.207418033081325	29.355065053870305	19.71157415279098
20	21.57398515722849	31.29578496899973	24.755194850291083	22.3750350234807
21	18.340569523704612	29.41661058918475	27.160978693749893	25.081841193360745
22	16.732482871422516	32.46706595430304	25.452072312411843	25.348378861862603
23	20.40030940401411	29.035842491324953	21.612301443805574	28.951546660855364
24	15.35884399763397	33.762635394190774	25.433872076287727	25.44464853188753
25	18.831017991891315	27.573118251244683	26.10344918422231	27.492414572641692
26	23.444777849354733	30.451389803557188	21.891770859027197	24.212061488060886
27	18.054634235123103	28.04752177442723	27.334838844093422	26.56300514635624
28	24.889301853310887	27.26155894601475	25.48512010958458	22.364019091089787
29	22.537400287851103	30.12450398369642	24.97934512676704	22.358750601685436
30	23.375808533515972	27.189236955100498	25.960960493513774	23.47399401786976
31	25.725315331064692	33.21519144972065	19.36553018964167	21.693963029572988
32	22.821898715685972	25.358915840671305	22.055333507353133	29.763851936289594
33	25.115846897697907	29.963336103281552	23.196679893768096	21.724137105252446
34	21.209501481163954	25.55432890221444	26.579768521733715	26.656401094887887
35	23.161955759057612	25.34047612775608	25.67670154247001	25.8208665707163
36	24.28103080389964	27.181094744202866	29.345725459017142	19.192148992880355
37	19.016373028207973	27.603052850133032	25.241332736237865	28.139241385421133
38	21.395095994271713	31.25339757697383	25.06435938760995	22.287147041144507
39	19.72594276025739	23.77429791391767	28.513783086712152	27.985976239112787
40	22.108018401396627	27.533365103920953	24.362931866458162	25.995684628224257
41	19.07815804031352	21.18818804675545	33.22381261420049	26.509841298730535
42	26.068485572720718	22.684917991172885	23.743405407864895	27.503191028241496
43	22.390122061320426	23.124118426062736	28.47450889297064	26.011250619646198
44	21.61469621171664	21.557461258642117	27.76350230017458	29.064340229466662
45	26.0890805767559	26.507446530819468	25.15104998599061	22.25242290643402
46	18.572143580704875	22.82477243717925	33.9539773502851	24.649106631830776
47	21.404914542707093	26.77039204745472	24.61965098652464	27.205042423313547
48	20.69055527483554	20.661818059902725	35.38173797886378	23.265888686397957
49	22.6889890966217	19.840412666406433	29.905622196624815	27.56497604034705
50	20.056181255193653	21.028457027087217	27.351841696262007	31.56352002145712
51	19.06283152568269	20.511905588669872	33.429762654552334	26.995500231095104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4954.0
1	3903.0
2	2852.0
3	1909.0
4	966.0
5	1123.5
6	1281.0
7	1200.0
8	1119.0
9	1084.0
10	1049.0
11	1062.5
12	1076.0
13	1079.5
14	1083.0
15	1182.5
16	1282.0
17	1210.0
18	1138.0
19	1165.0
20	1192.0
21	1249.0
22	1306.0
23	1532.0
24	1758.0
25	1850.5
26	2236.0
27	2529.0
28	2952.5
29	3376.0
30	3915.5
31	4455.0
32	5105.0
33	5755.0
34	6503.0
35	7251.0
36	7305.0
37	7359.0
38	8715.5
39	10072.0
40	11149.5
41	12227.0
42	16599.0
43	20971.0
44	35828.0
45	50685.0
46	51080.0
47	51475.0
48	50573.0
49	49671.0
50	44496.0
51	39321.0
52	34818.5
53	30316.0
54	26048.5
55	21781.0
56	18984.5
57	16188.0
58	14698.5
59	13209.0
60	12444.5
61	11680.0
62	10619.0
63	9558.0
64	8358.5
65	7159.0
66	6380.5
67	5602.0
68	4881.5
69	4161.0
70	3704.0
71	3247.0
72	2778.0
73	2309.0
74	2119.5
75	1439.0
76	948.0
77	792.0
78	636.0
79	500.5
80	365.0
81	312.5
82	260.0
83	155.0
84	50.0
85	33.5
86	17.0
87	12.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.369902463133094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.315520318254	28.846977377875486
2	8.847838961559413	6.435900800828493
3	3.2138177082388517	3.5065870974881084
4	1.5049472265888466	2.18939135372796
5	0.9529948033841926	1.7330164023477892
6	0.6828181439118529	1.4900417576479
7	0.5077581607771734	1.2926980347627952
8	0.38106189594303747	1.1087347190291874
9	0.3815522731790565	1.2489317064098095
>10	3.9219841337218413	31.309508802069548
>50	0.22503388083054562	5.139649055533409
>100	0.05279387233561188	3.8049339907243453
>500	0.0032996170209757423	0.8429274065113166
>1k	0.005939310637756336	3.9628628726959994
>5k	0.002639693616780594	7.087838622347861
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC	8442	2.02166307052352	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	8250	1.9756835266310164	TruSeq Adapter, Index 11 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGT	7401	1.772367730981352	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	5438	1.3022747900387235	TruSeq Adapter, Index 11 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4293	1.0280738642214489	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	2125	0.5088881811019285	TruSeq Adapter, Index 11 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	2083	0.4988301558754433	TruSeq Adapter, Index 11 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	1784	0.4272265953345132	RNA PCR Primer, Index 11 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT	1563	0.3743022244999126	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	1366	0.32712529665187495	Illumina PCR Primer Index 11 (100% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	1138	0.2725245882795269	TruSeq Adapter, Index 11 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCTC	1129	0.27036929715956576	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1030	0.2466610948399936	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	888	0.21265539050282942	TruSeq Adapter, Index 11 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.19206038646764548	No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	710	0.17002852168582083	RNA PCR Primer, Index 11 (96% over 26bp)
AAAAAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	595	0.14248869070853998	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.12380950100221036	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	475	0.1137514757757252	No Hit
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	428	0.10249606659370608	Illumina PCR Primer Index 11 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.594436475189007	0.0
2	0.0	0.0	0.0	2.1023667491265083	0.0
3	0.0	0.0	0.0	3.0581186224337067	0.0
4	0.0	0.0	0.0	7.313621200401363	0.0
5	0.0	0.0	0.0	7.97481662064721	0.0
6	0.0	0.0	0.0	10.442864429793786	0.0
7	0.0	0.0	0.0	12.52319931413847	0.0
8	0.0	0.0	0.0	13.859958762096571	0.0
9	0.0	0.0	0.0	17.229157736178	0.0
10	0.0	0.0	0.0	19.582496162384423	0.0
11	0.0	0.0	0.0	23.842069845800893	0.0
12	0.0	0.0	0.0	25.089983404258376	0.0
13	0.0	0.0	0.0	25.772731735703836	0.0
14	0.0	0.0	0.0	26.72201773565115	0.0
15	0.0	0.0	0.0	27.209592482344572	0.0
16	0.0	0.0	0.0	28.015192407627815	0.0
17	0.0	0.0	0.0	28.92855688890911	0.0
18	0.0	0.0	0.0	30.829763133505917	0.0
19	0.0	0.0	0.0	31.365472715211805	0.0
20	2.3947679110678988E-4	0.0	0.0	32.00822842254243	0.0
21	4.7895358221357976E-4	0.0	0.0	32.61075202896711	0.0
22	4.7895358221357976E-4	0.0	0.0	33.13784044619316	0.0
23	4.7895358221357976E-4	0.0	0.0	33.691510787232055	0.0
24	4.7895358221357976E-4	0.0	0.0	34.05958661516319	0.0
25	4.7895358221357976E-4	0.0	0.0	34.39198040121941	0.0
26	4.7895358221357976E-4	0.0	0.0	34.693242204431755	0.0
27	4.7895358221357976E-4	0.0	0.0	35.070897104007166	0.0
28	4.7895358221357976E-4	0.0	0.0	35.40592513476557	0.0
29	7.184303733203697E-4	0.0	0.0	35.74789799246606	0.0
30	7.184303733203697E-4	0.0	0.0	36.31378164985141	0.0
31	7.184303733203697E-4	0.0	0.0	36.64593595911652	0.0
32	7.184303733203697E-4	0.0	0.0	36.98264032741267	0.0
33	7.184303733203697E-4	0.0	0.0	37.310963008020074	0.0
34	7.184303733203697E-4	0.0	0.0	37.63162243131207	0.0
35	7.184303733203697E-4	0.0	0.0	37.987245466105655	0.0
36	7.184303733203697E-4	0.0	0.0	38.2674333117006	0.0
37	7.184303733203697E-4	0.0	0.0	38.56869511491294	0.0
38	7.184303733203697E-4	0.0	0.0	38.8634910447654	0.0
39	9.579071644271595E-4	0.0	0.0	39.193490062910556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.0281216E-4	45.0	12
CTTCGTG	20	7.0281216E-4	45.0	13
CTTCGCA	20	7.0281216E-4	45.0	35
TGTCACG	20	7.0281216E-4	45.0	1
TGCCCTT	20	7.0281216E-4	45.0	30
TACCCAA	20	7.0281216E-4	45.0	30
CGAACAG	20	7.0281216E-4	45.0	1
ACGCCTC	20	7.0281216E-4	45.0	45
AATTTGG	25	3.8865244E-5	45.0	2
TTTCGCG	20	7.0281216E-4	45.0	13
CGTGACA	20	7.0281216E-4	45.0	36
CGGTTAG	20	7.0281216E-4	45.0	1
TAAACGG	55	1.8189894E-12	45.0	2
CACGCCG	55	1.8189894E-12	45.0	26
TAAGTTA	25	3.8865244E-5	45.0	18
TAACGCG	20	7.0281216E-4	45.0	1
CGTTCCG	20	7.0281216E-4	45.0	43
TAGCCGT	35	1.209446E-7	45.0	44
TGTGATC	20	7.0281216E-4	45.0	37
CGTTAAG	20	7.0281216E-4	45.0	1
>>END_MODULE