FastQCFastQC Report
Sat 18 Jun 2016
SRR3548679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483053
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC114942.3794490459639004No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT106882.2125936491440896No Hit
AATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT97532.0190331081682547No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT72811.5072880201551382TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43140.8930697045665797No Hit
AATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT25340.5245801185377175No Hit
AAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT24610.5094679051781068No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT21990.4552295503806001No Hit
AACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG19890.41175605989404895TruSeq Adapter, Index 16 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT19480.4032683784181032No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTAT16970.3513072064556063No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCCCCTC13580.28112857181303086No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT11490.23786209794784424No Hit
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC10100.20908678757817464TruSeq Adapter, Index 16 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.20349733880133236No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.16726943006253972No Hit
AATCTGTCTCTTATACACATCTGACGCAACTCCCCTCGTATGCCGTCTTCT7370.15257124994565813No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAACTCCCCTCGTATGCC6990.14470461833380602No Hit
AATGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC6640.1374590365860475No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT5500.11385914175049115No Hit
ACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG5250.10868372621637792No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.10661356000273262No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC4950.10247322757544204TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATG4870.1008170946045258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG302.1628784E-645.0000041
CGAACAG351.2099736E-745.0000041
TCGGGAC750.045.0000045
GGTTTAC302.1628784E-645.0000049
CACGATT302.1628784E-645.00000436
CGATTAC302.1628784E-645.00000438
CCGTAGT600.045.00000427
CGTAGTG600.045.00000428
GCTTACG351.2099736E-745.0000041
GCTTAAG302.1628784E-645.0000041
TCCACGA302.1628784E-645.00000434
TAGGGCG351.2099736E-745.0000045
CGGCTAG351.2099736E-745.0000041
CAACTCG302.1628784E-645.0000041
CGATAGG302.1628784E-645.0000042
ATCGCGG302.1628784E-645.0000042
GATAGTC351.2099736E-745.0000049
GTAGTGC600.045.00000429
ACGCGAG207.029221E-445.01
AACGTCA207.029221E-445.040
Produced by FastQC (version 0.11.5)