##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483053
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.960927682883657	31.0	31.0	33.0	28.0	34.0
2	31.24290295267807	31.0	31.0	34.0	30.0	34.0
3	31.44901698157345	31.0	31.0	34.0	30.0	34.0
4	35.410333855705275	37.0	35.0	37.0	33.0	37.0
5	34.57894268330804	35.0	35.0	37.0	32.0	37.0
6	34.69485128961004	35.0	35.0	37.0	32.0	37.0
7	34.58934112819918	35.0	35.0	37.0	32.0	37.0
8	34.982964602227916	35.0	35.0	37.0	32.0	37.0
9	36.52900199357006	38.0	35.0	39.0	32.0	39.0
10	35.965840187308636	37.0	35.0	39.0	31.0	39.0
11	36.224778647477606	37.0	35.0	39.0	32.0	39.0
12	35.89502187130605	37.0	35.0	39.0	31.0	39.0
13	35.64509691483129	37.0	35.0	39.0	30.0	39.0
14	36.54892320304397	38.0	35.0	40.0	31.0	41.0
15	36.651947094832245	38.0	35.0	40.0	31.0	41.0
16	36.427017325221044	38.0	35.0	40.0	31.0	41.0
17	36.382517032292526	38.0	35.0	40.0	31.0	41.0
18	36.18608103044593	38.0	35.0	40.0	30.0	41.0
19	36.18631288906186	38.0	34.0	40.0	30.0	41.0
20	36.1955127077153	38.0	34.0	40.0	30.0	41.0
21	36.34966970498061	38.0	34.0	40.0	31.0	41.0
22	36.2843249084469	38.0	34.0	40.0	30.0	41.0
23	36.18055989715414	38.0	34.0	40.0	30.0	41.0
24	35.71795641472054	38.0	34.0	40.0	29.0	41.0
25	35.875088240834856	38.0	34.0	40.0	30.0	41.0
26	35.621621229968554	38.0	34.0	40.0	29.0	41.0
27	35.32897839367523	38.0	34.0	40.0	27.0	41.0
28	35.16931268411541	37.0	34.0	40.0	27.0	41.0
29	34.800918325732376	37.0	33.0	40.0	25.0	41.0
30	34.61781419430166	37.0	33.0	40.0	25.0	41.0
31	34.59272999029092	37.0	33.0	40.0	25.0	41.0
32	34.37146441487787	37.0	33.0	40.0	25.0	41.0
33	34.18932912123515	37.0	33.0	39.0	24.0	41.0
34	34.13916899387852	37.0	33.0	39.0	24.0	41.0
35	33.95154568960342	37.0	33.0	39.0	23.0	41.0
36	34.00628295445841	37.0	33.0	39.0	24.0	41.0
37	34.062686703115396	37.0	33.0	40.0	23.0	41.0
38	34.201706645026526	37.0	33.0	39.0	24.0	41.0
39	34.398664328758954	37.0	33.0	40.0	24.0	41.0
40	34.1647148449549	37.0	33.0	40.0	23.0	41.0
41	34.38578789491008	37.0	33.0	40.0	24.0	41.0
42	34.3403829393462	37.0	33.0	40.0	24.0	41.0
43	34.31159520797925	37.0	33.0	40.0	24.0	41.0
44	34.21463276286453	37.0	33.0	40.0	23.0	41.0
45	33.941056157398876	37.0	33.0	40.0	23.0	40.0
46	33.83763065336516	37.0	33.0	39.0	23.0	40.0
47	33.80113983351723	37.0	33.0	39.0	23.0	40.0
48	33.9770149445299	37.0	33.0	39.0	23.0	40.0
49	33.93841048497784	37.0	33.0	39.0	23.0	40.0
50	33.896197725715396	37.0	33.0	39.0	23.0	40.0
51	32.54241046013584	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	3.0
12	3.0
13	10.0
14	28.0
15	44.0
16	80.0
17	184.0
18	429.0
19	877.0
20	1564.0
21	2210.0
22	3153.0
23	3764.0
24	4219.0
25	4707.0
26	5432.0
27	6655.0
28	8697.0
29	11325.0
30	14796.0
31	19798.0
32	26380.0
33	35599.0
34	51617.0
35	58289.0
36	60805.0
37	69134.0
38	63712.0
39	29530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.10610015878175	42.51210529693429	17.386497961921364	23.9952965823626
2	16.88489668835511	42.923240306964246	18.67807466261466	21.513788342065983
3	17.11302900509882	30.361885755807332	29.465917818541648	23.059167420552196
4	24.91445038122111	29.98884180410845	17.790180373582196	27.306527441088246
5	14.295946821570304	40.96527710209853	19.659333447882528	25.07944262844864
6	19.90568322730632	32.904877932649214	26.72936510072394	20.46007373932053
7	64.53722469377067	15.865132811513435	11.676565511444913	7.9210769832709875
8	66.73760436225425	7.035666893694895	8.444000968837788	17.782727775213072
9	65.26198988516788	9.980892365848055	16.264674890746978	8.492442858237089
10	32.29645608245886	31.80417055685401	16.57519982279377	19.32417353789336
11	23.87750412480618	22.99312911833691	32.76762591268453	20.36174084417238
12	24.550515160862265	20.520522592758972	37.9264801170886	17.00248212929016
13	21.98164590634982	31.29635878464682	25.86300054031338	20.85899476868998
14	11.747779229194313	31.36674443591076	38.02150074629492	18.86397558860001
15	10.5414933765032	30.679863286223252	33.32036029172782	25.458283045545727
16	12.056233995027462	28.34202458115362	36.490405814682866	23.11133560913606
17	11.114308367818852	28.80532777976744	31.380614549542184	28.69974930287153
18	14.718467745775307	27.257671518446212	29.14317890583435	28.880681829944127
19	15.973195487865722	34.25793856988778	31.72757440694913	18.04129153529737
20	18.401293439850285	33.16302765949078	27.213369961474204	21.222308939184728
21	13.814840193519137	31.579557522673497	28.019906718310413	26.585695565496952
22	13.476989067452225	33.79960377018671	27.6785363096803	25.044870852680763
23	18.230918760467276	30.368510287691	21.441746557831127	29.9588243940106
24	12.12931086236914	35.91158734134764	26.13791861348548	25.821183182797746
25	14.942873763334457	29.006547935733764	28.137699175866832	27.912879125064947
26	19.15110764243261	34.73842414807485	23.022939511813405	23.087528697679137
27	12.73628359620994	32.81938006802566	27.452060125907508	26.99227620985689
28	18.159498026096514	32.19853722055343	27.828830376790954	21.81313437655909
29	16.26529594061107	35.93373811983364	24.694391712710615	23.10657422684467
30	17.939646374207385	34.43452374791172	21.170347767222232	26.45548211065866
31	19.678378977048066	34.860563954679925	21.615847536398697	23.845209531873316
32	18.849070391861762	30.19089002656023	17.250488041684868	33.70955153989314
33	18.86873697089139	31.841847581942357	21.798643213063578	27.49077223410268
34	12.766715039550524	31.19636975652775	21.987856404990758	34.049058798930965
35	13.595402574872736	33.01604585832196	21.280687626409524	32.107863940395774
36	15.609881317370972	29.680387038275303	29.3255605492565	25.384171095097223
37	14.679548620958776	29.730278044024157	21.779597683898043	33.81057565111903
38	17.401196142038245	25.175291324140414	28.497908097041112	28.925604436780226
39	15.55667804568029	21.955768828679254	26.824592746551623	35.66296037908884
40	18.622387191467602	25.38624126131087	19.840472991576494	36.150898555645036
41	15.295009036275523	19.661403614096177	31.420154724222808	33.62343262540549
42	21.868614831084788	23.895928604107624	22.899143572237417	31.336312992570175
43	17.864706357273423	23.51004962188414	29.317486901023283	29.30775711981915
44	19.695975389864053	22.447433304420013	26.545534340952237	31.3110569647637
45	24.959165971435844	25.304469695871884	26.32299147298537	23.413372859706904
46	15.930343047243264	22.89210500711102	35.00816680571283	26.16938513993288
47	19.46287467420759	24.837854231316232	24.03877007284915	31.660501021627024
48	18.748667330499966	18.810772316909325	36.35460291106773	26.08595744152298
49	20.94966804884764	17.209291734033325	30.724992909680722	31.116047307438315
50	18.4385564316959	18.645366036439064	26.999314774983286	35.916762756881745
51	15.871757343397103	19.7429681629138	36.06954102344877	28.315733470240325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5039.0
1	3984.5
2	2930.0
3	2015.5
4	1101.0
5	1285.0
6	1469.0
7	1494.5
8	1520.0
9	1547.5
10	1575.0
11	1723.5
12	1872.0
13	1868.5
14	1865.0
15	1917.0
16	1969.0
17	1844.0
18	1719.0
19	1764.5
20	1810.0
21	1722.5
22	1635.0
23	1675.5
24	1716.0
25	1965.0
26	2394.0
27	2574.0
28	3099.5
29	3625.0
30	4145.0
31	4665.0
32	5238.5
33	5812.0
34	7343.5
35	8875.0
36	9314.5
37	9754.0
38	10783.5
39	11813.0
40	13590.0
41	15367.0
42	22001.0
43	28635.0
44	47733.0
45	66831.0
46	68325.5
47	69820.0
48	67421.0
49	65022.0
50	58477.0
51	51932.0
52	44296.5
53	36661.0
54	30573.5
55	24486.0
56	20025.0
57	15564.0
58	12641.0
59	9718.0
60	8598.5
61	7479.0
62	6563.5
63	5648.0
64	4682.5
65	3717.0
66	3004.0
67	2291.0
68	1702.0
69	1113.0
70	993.0
71	873.0
72	773.0
73	673.0
74	772.0
75	611.0
76	351.0
77	277.5
78	204.0
79	159.5
80	115.0
81	98.0
82	81.0
83	46.5
84	12.0
85	9.0
86	6.0
87	4.5
88	3.0
89	8.0
90	13.0
91	11.5
92	10.0
93	7.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.819631304207334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19429555313927	29.15904763663014
2	9.098305525144951	6.699925098577392
3	3.458597906067	3.8203289919267127
4	1.723390108008111	2.538183534807068
5	1.0427809371597208	1.9197404818638357
6	0.7382727439680956	1.6309758140910444
7	0.5442538071148	1.4027457159714678
8	0.418126182678388	1.2316201507883113
9	0.3616949157826731	1.198572609935191
>10	2.993952791739678	23.31551458191987
>50	0.3529217956931652	8.55159582822794
>100	0.06098488629577893	4.943504753294977
>500	0.004517398984872513	1.13956132198384
>1k	0.005646748731090642	4.2952376119073445
>5k	0.0011293497462181283	3.5415594889021595
>10k+	0.0011293497462181283	4.611886379172696
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC	11494	2.3794490459639004	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT	10688	2.2125936491440896	No Hit
AATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	9753	2.0190331081682547	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	7281	1.5072880201551382	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4314	0.8930697045665797	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT	2534	0.5245801185377175	No Hit
AAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	2461	0.5094679051781068	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	2199	0.4552295503806001	No Hit
AACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	1989	0.41175605989404895	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT	1948	0.4032683784181032	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTAT	1697	0.3513072064556063	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCCCCTC	1358	0.28112857181303086	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	1149	0.23786209794784424	No Hit
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1010	0.20908678757817464	TruSeq Adapter, Index 16 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	983	0.20349733880133236	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	808	0.16726943006253972	No Hit
AATCTGTCTCTTATACACATCTGACGCAACTCCCCTCGTATGCCGTCTTCT	737	0.15257124994565813	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAACTCCCCTCGTATGCC	699	0.14470461833380602	No Hit
AATGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	664	0.1374590365860475	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	550	0.11385914175049115	No Hit
ACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	525	0.10868372621637792	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	515	0.10661356000273262	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	495	0.10247322757544204	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATG	487	0.1008170946045258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0701662136452935E-4	0.0	0.0	1.92877386125332	0.0
2	2.0701662136452935E-4	0.0	0.0	2.5558272073664794	0.0
3	4.140332427290587E-4	0.0	0.0	3.696281774463672	0.0
4	4.140332427290587E-4	0.0	0.0	9.20458003573107	0.0
5	4.140332427290587E-4	0.0	0.0	9.988344964217177	0.0
6	4.140332427290587E-4	0.0	0.0	13.511560843220103	0.0
7	4.140332427290587E-4	0.0	0.0	16.45720034861599	0.0
8	4.140332427290587E-4	0.0	0.0	18.571668119233294	0.0
9	4.140332427290587E-4	0.0	0.0	23.38335544960905	0.0
10	4.140332427290587E-4	0.0	0.0	26.96018863354539	0.0
11	4.140332427290587E-4	0.0	0.0	32.54011464580491	0.0
12	4.140332427290587E-4	0.0	0.0	34.301412060374325	0.0
13	4.140332427290587E-4	0.0	0.0	35.18081866793085	0.0
14	4.140332427290587E-4	0.0	0.0	36.2380525532395	0.0
15	4.140332427290587E-4	0.0	0.0	36.81335174401153	0.0
16	4.140332427290587E-4	0.0	0.0	37.72774416057865	0.0
17	4.140332427290587E-4	0.0	0.0	38.82244805435428	0.0
18	6.210498640935881E-4	0.0	0.0	40.99860677813822	0.0
19	6.210498640935881E-4	0.0	0.0	41.65650560083469	0.0
20	6.210498640935881E-4	0.0	0.0	42.35373758159042	0.0
21	6.210498640935881E-4	0.0	0.0	42.93938760343068	0.0
22	6.210498640935881E-4	0.0	0.0	43.56416376670883	0.0
23	6.210498640935881E-4	0.0	0.0	44.12372969425715	0.0
24	6.210498640935881E-4	0.0	0.0	44.510022709723366	0.0
25	6.210498640935881E-4	0.0	0.0	44.83938615431433	0.0
26	6.210498640935881E-4	0.0	0.0	45.19421264333313	0.0
27	6.210498640935881E-4	0.0	0.0	45.58319687487708	0.0
28	6.210498640935881E-4	0.0	0.0	45.92208308405082	0.0
29	6.210498640935881E-4	0.0	0.0	46.27649553982689	0.0
30	6.210498640935881E-4	0.0	0.0	46.900443636619585	0.0
31	6.210498640935881E-4	0.0	0.0	47.288392785056715	0.0
32	6.210498640935881E-4	0.0	0.0	47.62748601085181	0.0
33	6.210498640935881E-4	0.0	0.0	47.94836177396683	0.0
34	6.210498640935881E-4	0.0	0.0	48.240255210090815	0.0
35	6.210498640935881E-4	0.0	0.0	48.55264329172989	0.0
36	6.210498640935881E-4	0.0	0.0	48.86730855620398	0.0
37	6.210498640935881E-4	0.0	0.0	49.17348613920212	0.0
38	6.210498640935881E-4	0.0	0.0	49.4753163731516	0.0
39	6.210498640935881E-4	0.0	0.0	49.75913616104237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	30	2.1628784E-6	45.000004	1
CGAACAG	35	1.2099736E-7	45.000004	1
TCGGGAC	75	0.0	45.000004	5
GGTTTAC	30	2.1628784E-6	45.000004	9
CACGATT	30	2.1628784E-6	45.000004	36
CGATTAC	30	2.1628784E-6	45.000004	38
CCGTAGT	60	0.0	45.000004	27
CGTAGTG	60	0.0	45.000004	28
GCTTACG	35	1.2099736E-7	45.000004	1
GCTTAAG	30	2.1628784E-6	45.000004	1
TCCACGA	30	2.1628784E-6	45.000004	34
TAGGGCG	35	1.2099736E-7	45.000004	5
CGGCTAG	35	1.2099736E-7	45.000004	1
CAACTCG	30	2.1628784E-6	45.000004	1
CGATAGG	30	2.1628784E-6	45.000004	2
ATCGCGG	30	2.1628784E-6	45.000004	2
GATAGTC	35	1.2099736E-7	45.000004	9
GTAGTGC	60	0.0	45.000004	29
ACGCGAG	20	7.029221E-4	45.0	1
AACGTCA	20	7.029221E-4	45.0	40
>>END_MODULE